Predictive toxicological effects of Artemisia absinthium essential oil on hepatic stellate cells
In conclusion, the hepatic stellate cells incubated with the essential oil present an antifibrotic potential, supporting its popular use; however, the combined results suggest that the essential oil of Artemisia absinthium should be used with caution.PMID:38000518 | DOI:10.1016/j.tiv.2023.105738 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - November 24, 2023 Category: Toxicology Authors: I I Barreto L R Gon çalves A F Corr êa M A Marin-Morales K C M Moraes Source Type: research
Predictive toxicological effects of Artemisia absinthium essential oil on hepatic stellate cells
In conclusion, the hepatic stellate cells incubated with the essential oil present an antifibrotic potential, supporting its popular use; however, the combined results suggest that the essential oil of Artemisia absinthium should be used with caution.PMID:38000518 | DOI:10.1016/j.tiv.2023.105738 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - November 24, 2023 Category: Toxicology Authors: I I Barreto L R Gon çalves A F Corr êa M A Marin-Morales K C M Moraes Source Type: research
Predictive toxicological effects of Artemisia absinthium essential oil on hepatic stellate cells
In conclusion, the hepatic stellate cells incubated with the essential oil present an antifibrotic potential, supporting its popular use; however, the combined results suggest that the essential oil of Artemisia absinthium should be used with caution.PMID:38000518 | DOI:10.1016/j.tiv.2023.105738 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - November 24, 2023 Category: Toxicology Authors: I I Barreto L R Gon çalves A F Corr êa M A Marin-Morales K C M Moraes Source Type: research
Predictive toxicological effects of Artemisia absinthium essential oil on hepatic stellate cells
In conclusion, the hepatic stellate cells incubated with the essential oil present an antifibrotic potential, supporting its popular use; however, the combined results suggest that the essential oil of Artemisia absinthium should be used with caution.PMID:38000518 | DOI:10.1016/j.tiv.2023.105738 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - November 24, 2023 Category: Toxicology Authors: I I Barreto L R Gon çalves A F Corr êa M A Marin-Morales K C M Moraes Source Type: research
Evaluation of the acute toxicity of ellagic acid and gallic acid incorporated in Poloxamer407 ® gel, in Zophobas morio larvae
In this study, the combination of these substances incorporated into a poloxamer gel was tested to verify the individual effect of the substances, in addition to taking advantage of a probable complementary effect, aiming to provide additional therapeutic benefits. As a result of the incorporation, formulations containing GA, EA and GA + EA were obtained, which were evaluated for the effects of the Freeze-thaw cycle on pH, which revealed a significant decrease (p < 0.05) in most samples, including the vehicle (without drug) and the gel containing both drugs. No sample showed variation outside the normal pH range for the...
Source: Toxicology in Vitro - November 22, 2023 Category: Toxicology Authors: Jamilly Erica Sousa Campelo Matheus Oliveira do Nascimento Andr é Luis Menezes Carvalho Hayana Sara Pereira Santos Jos é Otavio Carvalho Sena de Almeida Michel Mualem de Moraes Alves Daniel Dias Rufino Arcanjo Jos é Moreira Tavares Neto Maria Christina Source Type: research
Evaluation of the acute toxicity of ellagic acid and gallic acid incorporated in Poloxamer407 ® gel, in Zophobas morio larvae
In this study, the combination of these substances incorporated into a poloxamer gel was tested to verify the individual effect of the substances, in addition to taking advantage of a probable complementary effect, aiming to provide additional therapeutic benefits. As a result of the incorporation, formulations containing GA, EA and GA + EA were obtained, which were evaluated for the effects of the Freeze-thaw cycle on pH, which revealed a significant decrease (p < 0.05) in most samples, including the vehicle (without drug) and the gel containing both drugs. No sample showed variation outside the normal pH range for the...
Source: Toxicology in Vitro - November 22, 2023 Category: Toxicology Authors: Jamilly Erica Sousa Campelo Matheus Oliveira do Nascimento Andr é Luis Menezes Carvalho Hayana Sara Pereira Santos Jos é Otavio Carvalho Sena de Almeida Michel Mualem de Moraes Alves Daniel Dias Rufino Arcanjo Jos é Moreira Tavares Neto Maria Christina Source Type: research
Life cell imaging of amiodarone sequestration into lamellar bodies of alveolar type II cells
In this study, we used live cell imaging of primary rat alveolar type II cells to show that amiodarone specifically accumulates in large amounts in lamellar bodies, the surfactant storage organelles. Fluorescence imaging and correlation, and colocalization studies combined with confocal Raman microscopy identified these organelles as a major target for sequestration. Accumulation was rapid, on the order of a few hours, while storage was much more persistent. Partial uptake was observed in chemically fixed, dead cells, or cells treated with bafilomycin A1. Not only was uptake pH dependent, but intraluminal pH, measured with...
Source: Toxicology in Vitro - November 20, 2023 Category: Toxicology Authors: Thomas Haller Alexander Jesacher Alberto Hidalgo Christina Schmidt Source Type: research
In vitro anticancer effects of recombinant anisoplin through activation of SAPK/JNK and downregulation of NF κB
Toxicol In Vitro. 2023 Nov 18:105737. doi: 10.1016/j.tiv.2023.105737. Online ahead of print.ABSTRACTEmerging chemotherapeutic resistance is considered as one of the major obstacles in breast cancer therapy. Fungal ribotoxins possess promising therapeutic potential against cancer owing to their ribosome-targeted protein synthesis inhibitory action. Though the entomopathogenic ribotoxin anisoplin was characterized in the earlier study, its therapeutic efficacy against cancer cells remained unexplored. In the current study, recombinant anisoplin has been successfully produced in Escherichia coli BL21(DE3) expression system an...
Source: Toxicology in Vitro - November 20, 2023 Category: Toxicology Authors: Arupam Patra Thirukumaran Kandasamy Siddhartha Sankar Ghosh Gurvinder Kaur Saini Source Type: research
Applications of Premature Chromosome Condensation technique for genetic analysis
We present here the various approaches to obtain PCC, its applications and the endpoints which are used while using PCC as a cytogenetic technique.PMID:37984482 | DOI:10.1016/j.tiv.2023.105736 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - November 20, 2023 Category: Toxicology Authors: C H Anjali Ravi Maddaly Source Type: research
Life cell imaging of amiodarone sequestration into lamellar bodies of alveolar type II cells
In this study, we used live cell imaging of primary rat alveolar type II cells to show that amiodarone specifically accumulates in large amounts in lamellar bodies, the surfactant storage organelles. Fluorescence imaging and correlation, and colocalization studies combined with confocal Raman microscopy identified these organelles as a major target for sequestration. Accumulation was rapid, on the order of a few hours, while storage was much more persistent. Partial uptake was observed in chemically fixed, dead cells, or cells treated with bafilomycin A1. Not only was uptake pH dependent, but intraluminal pH, measured with...
Source: Toxicology in Vitro - November 20, 2023 Category: Toxicology Authors: Thomas Haller Alexander Jesacher Alberto Hidalgo Christina Schmidt Source Type: research
In vitro anticancer effects of recombinant anisoplin through activation of SAPK/JNK and downregulation of NF κB
Toxicol In Vitro. 2023 Nov 18:105737. doi: 10.1016/j.tiv.2023.105737. Online ahead of print.ABSTRACTEmerging chemotherapeutic resistance is considered as one of the major obstacles in breast cancer therapy. Fungal ribotoxins possess promising therapeutic potential against cancer owing to their ribosome-targeted protein synthesis inhibitory action. Though the entomopathogenic ribotoxin anisoplin was characterized in the earlier study, its therapeutic efficacy against cancer cells remained unexplored. In the current study, recombinant anisoplin has been successfully produced in Escherichia coli BL21(DE3) expression system an...
Source: Toxicology in Vitro - November 20, 2023 Category: Toxicology Authors: Arupam Patra Thirukumaran Kandasamy Siddhartha Sankar Ghosh Gurvinder Kaur Saini Source Type: research
Applications of Premature Chromosome Condensation technique for genetic analysis
We present here the various approaches to obtain PCC, its applications and the endpoints which are used while using PCC as a cytogenetic technique.PMID:37984482 | DOI:10.1016/j.tiv.2023.105736 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - November 20, 2023 Category: Toxicology Authors: C H Anjali Ravi Maddaly Source Type: research
Cytotoxic and genotoxic effects of triphenyl phosphate on root tip cells of Allium cepa L
In this study, we evaluated genotoxicity and cytotoxicity of TPhP on Allium cepa on the cells of root tips. A 10 mg/mL stock solution of TPhP was dissolved in DMSO and dilutions of 2, 4, 6, 8 and 10 mg/mL was made in distilled water. Onion bulbs, rooted in test tubes, were exposed to these concentrations of TPhP for 24 h. Distilled water was used as a negative control, 0.7% hydrogen peroxide was used as positive control, and 0.5% DMSO was used as solvent control. Significant inhibition of onion root growth was observed following treatment with the 6, 8 and 10 mg/mL TPhP ranges in comparison with the negative and solvent co...
Source: Toxicology in Vitro - November 19, 2023 Category: Toxicology Authors: Özlem Sultan Aslantürk Source Type: research
Skin barrier function for regulatory skin absorption tests and effects on testosterone and sucrose absorption
Toxicol In Vitro. 2023 Nov 15:105735. doi: 10.1016/j.tiv.2023.105735. Online ahead of print.ABSTRACTIn vitro absorption through human skin is a critical component in the safety assessment of chemicals, crop protection products, consumer healthcare products and cosmetics. A barrier integrity assay is used to identify skin samples which are potentially damaged. A retrospective analysis of 9978 electrical resistance (ER) measurements generated in a single laboratory (DTL) over a 15-year period was performed. Skin absorption experiments were performed using two model penetrants, testosterone and sucrose, utilising no ER accept...
Source: Toxicology in Vitro - November 17, 2023 Category: Toxicology Authors: Thomas Brackin Heather Gayes Ian Johnson David Fox Clive Roper Source Type: research
Skin barrier function for regulatory skin absorption tests and effects on testosterone and sucrose absorption
Toxicol In Vitro. 2023 Nov 15:105735. doi: 10.1016/j.tiv.2023.105735. Online ahead of print.ABSTRACTIn vitro absorption through human skin is a critical component in the safety assessment of chemicals, crop protection products, consumer healthcare products and cosmetics. A barrier integrity assay is used to identify skin samples which are potentially damaged. A retrospective analysis of 9978 electrical resistance (ER) measurements generated in a single laboratory (DTL) over a 15-year period was performed. Skin absorption experiments were performed using two model penetrants, testosterone and sucrose, utilising no ER accept...
Source: Toxicology in Vitro - November 17, 2023 Category: Toxicology Authors: Thomas Brackin Heather Gayes Ian Johnson David Fox Clive Roper Source Type: research
