Everolimus prevents doxorubicin-induced apoptosis in H9c2 cardiomyocytes but not in MCF-7 cancer cells: Cardioprotective roles of autophagy, mitophagy, and AKT
Toxicol In Vitro. 2023 Sep 20:105698. doi: 10.1016/j.tiv.2023.105698. Online ahead of print.ABSTRACTCardiotoxicity is a severe side effect of the chemotherapeutic agent doxorubicin (DOX). We recently showed that DOX-induced cardiomyocyte apoptosis and death were attenuated through autophagy pre-induction. Herein, we assessed how the autophagy/mitophagy-inducing antitumor drug everolimus (EVL) affected DOX-induced cytotoxicity in the rat cardiomyocyte cell line H9c2 and human breast cancer cell line MCF-7. Apoptosis was assessed using annexin V assay. Autophagy and mitophagy were assessed using fluorescence assays. Cellular...
Source: Toxicology in Vitro - September 22, 2023 Category: Toxicology Authors: Syu-Ichi Kanno Akiyoshi Hara Source Type: research
Everolimus prevents doxorubicin-induced apoptosis in H9c2 cardiomyocytes but not in MCF-7 cancer cells: Cardioprotective roles of autophagy, mitophagy, and AKT
Toxicol In Vitro. 2023 Sep 20:105698. doi: 10.1016/j.tiv.2023.105698. Online ahead of print.ABSTRACTCardiotoxicity is a severe side effect of the chemotherapeutic agent doxorubicin (DOX). We recently showed that DOX-induced cardiomyocyte apoptosis and death were attenuated through autophagy pre-induction. Herein, we assessed how the autophagy/mitophagy-inducing antitumor drug everolimus (EVL) affected DOX-induced cytotoxicity in the rat cardiomyocyte cell line H9c2 and human breast cancer cell line MCF-7. Apoptosis was assessed using annexin V assay. Autophagy and mitophagy were assessed using fluorescence assays. Cellular...
Source: Toxicology in Vitro - September 22, 2023 Category: Toxicology Authors: Syu-Ichi Kanno Akiyoshi Hara Source Type: research
Everolimus prevents doxorubicin-induced apoptosis in H9c2 cardiomyocytes but not in MCF-7 cancer cells: Cardioprotective roles of autophagy, mitophagy, and AKT
Toxicol In Vitro. 2023 Sep 20:105698. doi: 10.1016/j.tiv.2023.105698. Online ahead of print.ABSTRACTCardiotoxicity is a severe side effect of the chemotherapeutic agent doxorubicin (DOX). We recently showed that DOX-induced cardiomyocyte apoptosis and death were attenuated through autophagy pre-induction. Herein, we assessed how the autophagy/mitophagy-inducing antitumor drug everolimus (EVL) affected DOX-induced cytotoxicity in the rat cardiomyocyte cell line H9c2 and human breast cancer cell line MCF-7. Apoptosis was assessed using annexin V assay. Autophagy and mitophagy were assessed using fluorescence assays. Cellular...
Source: Toxicology in Vitro - September 22, 2023 Category: Toxicology Authors: Syu-Ichi Kanno Akiyoshi Hara Source Type: research
Everolimus prevents doxorubicin-induced apoptosis in H9c2 cardiomyocytes but not in MCF-7 cancer cells: Cardioprotective roles of autophagy, mitophagy, and AKT
Toxicol In Vitro. 2023 Sep 20:105698. doi: 10.1016/j.tiv.2023.105698. Online ahead of print.ABSTRACTCardiotoxicity is a severe side effect of the chemotherapeutic agent doxorubicin (DOX). We recently showed that DOX-induced cardiomyocyte apoptosis and death were attenuated through autophagy pre-induction. Herein, we assessed how the autophagy/mitophagy-inducing antitumor drug everolimus (EVL) affected DOX-induced cytotoxicity in the rat cardiomyocyte cell line H9c2 and human breast cancer cell line MCF-7. Apoptosis was assessed using annexin V assay. Autophagy and mitophagy were assessed using fluorescence assays. Cellular...
Source: Toxicology in Vitro - September 22, 2023 Category: Toxicology Authors: Syu-Ichi Kanno Akiyoshi Hara Source Type: research
In silico and in vitro evaluation of a safety profile of a cosmetic ingredient: 4-methoxychalcone (4-MC)
Toxicol In Vitro. 2023 Sep 16;93:105696. doi: 10.1016/j.tiv.2023.105696. Online ahead of print.ABSTRACTChalcone is an important scaffold within medicinal and cosmetic chemistry. The structure enables multiple modifications which may result in obtaining compounds with desirable bioactivity. One of the chalcone derivatives, 4-methoxychalcone is a known cosmetic ingredient indexed in Cosing database as an antioxidant, bleaching, and skin conditioning substance. We investigated its in silico and in vitro safety profile. In silico study using Derek Nexus showed its potential of skin sensitisation, equivocal nature of chromosome...
Source: Toxicology in Vitro - September 18, 2023 Category: Toxicology Authors: Agnieszka Gunia-Krzy żak Justyna Popi ół Karolina S łoczyńska Dorota Żelaszczyk Katarzyna Orze ł Paulina Koczurkiewicz-Adamczyk Katarzyna W ójcik-Pszczoła Patryk Kasza Magda Borczuch-Kosta ńska El żbieta Pękala Source Type: research
In silico and in vitro evaluation of a safety profile of a cosmetic ingredient: 4-methoxychalcone (4-MC)
Toxicol In Vitro. 2023 Sep 16:105696. doi: 10.1016/j.tiv.2023.105696. Online ahead of print.ABSTRACTChalcone is an important scaffold within medicinal and cosmetic chemistry. The structure enables multiple modifications which may result in obtaining compounds with desirable bioactivity. One of the chalcone derivatives, 4-methoxychalcone is a known cosmetic ingredient indexed in Cosing database as an antioxidant, bleaching, and skin conditioning substance. We investigated its in silico and in vitro safety profile. In silico study using Derek Nexus showed its potential of skin sensitisation, equivocal nature of chromosome da...
Source: Toxicology in Vitro - September 18, 2023 Category: Toxicology Authors: Agnieszka Gunia-Krzy żak Justyna Popi ół Karolina S łoczyńska Dorota Żelaszczyk Katarzyna Orze ł Paulina Koczurkiewicz-Adamczyk Katarzyna W ójcik-Pszczoła Patryk Kasza Magda Borczuch-Kosta ńska El żbieta Pękala Source Type: research
In silico and in vitro evaluation of a safety profile of a cosmetic ingredient: 4-methoxychalcone (4-MC)
Toxicol In Vitro. 2023 Sep 16:105696. doi: 10.1016/j.tiv.2023.105696. Online ahead of print.ABSTRACTChalcone is an important scaffold within medicinal and cosmetic chemistry. The structure enables multiple modifications which may result in obtaining compounds with desirable bioactivity. One of the chalcone derivatives, 4-methoxychalcone is a known cosmetic ingredient indexed in Cosing database as an antioxidant, bleaching, and skin conditioning substance. We investigated its in silico and in vitro safety profile. In silico study using Derek Nexus showed its potential of skin sensitisation, equivocal nature of chromosome da...
Source: Toxicology in Vitro - September 18, 2023 Category: Toxicology Authors: Agnieszka Gunia-Krzy żak Justyna Popi ół Karolina S łoczyńska Dorota Żelaszczyk Katarzyna Orze ł Paulina Koczurkiewicz-Adamczyk Katarzyna W ójcik-Pszczoła Patryk Kasza Magda Borczuch-Kosta ńska El żbieta Pękala Source Type: research
In silico and in vitro evaluation of a safety profile of a cosmetic ingredient: 4-methoxychalcone (4-MC)
Toxicol In Vitro. 2023 Sep 16:105696. doi: 10.1016/j.tiv.2023.105696. Online ahead of print.ABSTRACTChalcone is an important scaffold within medicinal and cosmetic chemistry. The structure enables multiple modifications which may result in obtaining compounds with desirable bioactivity. One of the chalcone derivatives, 4-methoxychalcone is a known cosmetic ingredient indexed in Cosing database as an antioxidant, bleaching, and skin conditioning substance. We investigated its in silico and in vitro safety profile. In silico study using Derek Nexus showed its potential of skin sensitisation, equivocal nature of chromosome da...
Source: Toxicology in Vitro - September 18, 2023 Category: Toxicology Authors: Agnieszka Gunia-Krzy żak Justyna Popi ół Karolina S łoczyńska Dorota Żelaszczyk Katarzyna Orze ł Paulina Koczurkiewicz-Adamczyk Katarzyna W ójcik-Pszczoła Patryk Kasza Magda Borczuch-Kosta ńska El żbieta Pękala Source Type: research
Bisphenol S and F affect cell cycle distribution and steroidogenic activity of human ovarian granulosa cells, but not primary granulosa tumour cells
Toxicol In Vitro. 2023 Sep 15:105697. doi: 10.1016/j.tiv.2023.105697. Online ahead of print.ABSTRACTBisphenol S (BPS) and F (BPF), a new generation of bisphenols (BPs), are the main substitutes for bisphenol A (BPA). Both have been detected in human body fluids. Importantly, bisphenols are structurally similar to oestrogen, the main sex hormone in females. Because bisphenols bind to nuclear oestrogen receptors (ESR1 and ESR2) and to membrane G-coupled receptor 30 (GPR30), they can disrupt ovarian function. Here, we reveal the molecular mechanism underlying the effects of BPS and BPF on the cell cycle and steroidogenesis in...
Source: Toxicology in Vitro - September 17, 2023 Category: Toxicology Authors: Paulina G łód Norbert Borski Justyna Gogola-Mruk Ma łgorzata Opydo Anna Ptak Source Type: research
The widely disregarded health risks posed by phthalates - A global call for action
Toxicol In Vitro. 2023 Sep 13:105695. doi: 10.1016/j.tiv.2023.105695. Online ahead of print.ABSTRACTThe 21st century has seen an incredible surge in the usage of phthalate-containing microplastics. These chemicals are now seen to be infiltrating the daily lives of billions of people through various routes such as food, packaging, and personal care items and are hence easily absorbed by the human body. Recent literature has highlighted significant findings regarding their oncogenicity and toxicity. Perhaps the most alarming discovery has been the impact of phthalates on the developing fetus, affecting human lives well befor...
Source: Toxicology in Vitro - September 15, 2023 Category: Toxicology Authors: Wania Sultan Fatima Hizbullah Arain Sakina Mansoor Ayesha Amir Source Type: research
The widely disregarded health risks posed by phthalates - A global call for action
Toxicol In Vitro. 2023 Sep 13:105695. doi: 10.1016/j.tiv.2023.105695. Online ahead of print.ABSTRACTThe 21st century has seen an incredible surge in the usage of phthalate-containing microplastics. These chemicals are now seen to be infiltrating the daily lives of billions of people through various routes such as food, packaging, and personal care items and are hence easily absorbed by the human body. Recent literature has highlighted significant findings regarding their oncogenicity and toxicity. Perhaps the most alarming discovery has been the impact of phthalates on the developing fetus, affecting human lives well befor...
Source: Toxicology in Vitro - September 15, 2023 Category: Toxicology Authors: Wania Sultan Fatima Hizbullah Arain Sakina Mansoor Ayesha Amir Source Type: research
The widely disregarded health risks posed by phthalates - A global call for action
Toxicol In Vitro. 2023 Sep 13:105695. doi: 10.1016/j.tiv.2023.105695. Online ahead of print.ABSTRACTThe 21st century has seen an incredible surge in the usage of phthalate-containing microplastics. These chemicals are now seen to be infiltrating the daily lives of billions of people through various routes such as food, packaging, and personal care items and are hence easily absorbed by the human body. Recent literature has highlighted significant findings regarding their oncogenicity and toxicity. Perhaps the most alarming discovery has been the impact of phthalates on the developing fetus, affecting human lives well befor...
Source: Toxicology in Vitro - September 15, 2023 Category: Toxicology Authors: Wania Sultan Fatima Hizbullah Arain Sakina Mansoor Ayesha Amir Source Type: research
Anticancer effect of umbelliferone on MKN-45 and MIA PaCa-2 cell lines
In this study, the anticancer activity of umbelliferone (7-hydroxycoumarin-UMB) was investigated in MKN-45 human gastric cancer and MIA PaCa-2 human pancreatic cancer cells. The cytotoxic effect of UMB on MKN-45 and MIA PaCa-2 cells was determined by WST-8 cell viability assay; the effect on colony formation and migration potential by colony forming assay and wound healing/cell migration assay. Apoptotic effect of UMB was determined by measuring the change in mitochondrial membrane potentials, reactive oxygen species levels, and Caspase-3 activities in cells. Anticancer drugs cisplatin and gemcitabine were used as positive...
Source: Toxicology in Vitro - September 13, 2023 Category: Toxicology Authors: Özlem Sultan Aslantürk T ülay Aşkin Çelik Source Type: research
Anticancer effect of umbelliferone on MKN-45 and MIA PaCa-2 cell lines
In this study, the anticancer activity of umbelliferone (7-hydroxycoumarin-UMB) was investigated in MKN-45 human gastric cancer and MIA PaCa-2 human pancreatic cancer cells. The cytotoxic effect of UMB on MKN-45 and MIA PaCa-2 cells was determined by WST-8 cell viability assay; the effect on colony formation and migration potential by colony forming assay and wound healing/cell migration assay. Apoptotic effect of UMB was determined by measuring the change in mitochondrial membrane potentials, reactive oxygen species levels, and Caspase-3 activities in cells. Anticancer drugs cisplatin and gemcitabine were used as positive...
Source: Toxicology in Vitro - September 13, 2023 Category: Toxicology Authors: Özlem Sultan Aslantürk T ülay Aşkin Çelik Source Type: research
Effect of WISP1 on paraquat-induced EMT
CONCLUSION: Knockdown of WISP1 inhibited PQ-induced EMT in A549 cells. This conclusion might provide a new therapeutic target for PQ poisoning treatment.PMID:37689312 | DOI:10.1016/j.tiv.2023.105693 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - September 9, 2023 Category: Toxicology Authors: Jingyan Liu Shengnan Lv Wanling Ma Dong Yang Xuchang Zhang Source Type: research
