Fragrances as a trigger of immune responses in different environments
Toxicol In Vitro. 2023 Dec 22:105769. doi: 10.1016/j.tiv.2023.105769. Online ahead of print.ABSTRACTFragrances can cause allergic skin reactions, expressed as allergic contact dermatitis and reactions in the respiratory tract that range from acute temporary upper airway irritation to obstructive lung disease. These adverse health effects may result from the stimulation of a specific (adaptive) immune response. Th1 cells, which essentially produce interleukin-2 (IL-2) and interferon-γ (IFN-γ), play a key role in allergic contact dermatitis and also on allergic sensitization to common allergens (e.g., nickel and fragrance)...
Source: Toxicology in Vitro - December 24, 2023 Category: Toxicology Authors: M Macchione K Yoshizaki D P Frias K Maier J Smelan C M Prado T Mauad Source Type: research
Comparison of in vitro thyroxine (T4) metabolism between Wistar rat and human hepatocyte cultures
Toxicol In Vitro. 2023 Dec 22:105763. doi: 10.1016/j.tiv.2023.105763. Online ahead of print.ABSTRACTIn vitro assays remain relatively new in exploring human relevance of liver-, in particular nuclear receptor-mediated perturbations of the hypothalamus-pituitary-thyroid axis seen in rodents, mainly in the rat. Consistent with in vivo data, we confirm that thyroid hormone thyroxine metabolism was 9 times higher in primary rat hepatocytes (PRH) than in primary human hepatocytes (PHH) cultured in a 2D sandwich configuration. In addition, thyroxine glucuronide (T4-G) was by far the major metabolite formed in both species (99.1%...
Source: Toxicology in Vitro - December 24, 2023 Category: Toxicology Authors: Audrey Baze Lucille Wiss Liliia Horbal Klaus Biemel Laure Asselin Lysiane Richert Source Type: research
Fragrances as a trigger of immune responses in different environments
Toxicol In Vitro. 2023 Dec 22:105769. doi: 10.1016/j.tiv.2023.105769. Online ahead of print.ABSTRACTFragrances can cause allergic skin reactions, expressed as allergic contact dermatitis and reactions in the respiratory tract that range from acute temporary upper airway irritation to obstructive lung disease. These adverse health effects may result from the stimulation of a specific (adaptive) immune response. Th1 cells, which essentially produce interleukin-2 (IL-2) and interferon-γ (IFN-γ), play a key role in allergic contact dermatitis and also on allergic sensitization to common allergens (e.g., nickel and fragrance)...
Source: Toxicology in Vitro - December 24, 2023 Category: Toxicology Authors: M Macchione K Yoshizaki D P Frias K Maier J Smelan C M Prado T Mauad Source Type: research
Comparison of in vitro thyroxine (T4) metabolism between Wistar rat and human hepatocyte cultures
Toxicol In Vitro. 2023 Dec 22:105763. doi: 10.1016/j.tiv.2023.105763. Online ahead of print.ABSTRACTIn vitro assays remain relatively new in exploring human relevance of liver-, in particular nuclear receptor-mediated perturbations of the hypothalamus-pituitary-thyroid axis seen in rodents, mainly in the rat. Consistent with in vivo data, we confirm that thyroid hormone thyroxine metabolism was 9 times higher in primary rat hepatocytes (PRH) than in primary human hepatocytes (PHH) cultured in a 2D sandwich configuration. In addition, thyroxine glucuronide (T4-G) was by far the major metabolite formed in both species (99.1%...
Source: Toxicology in Vitro - December 24, 2023 Category: Toxicology Authors: Audrey Baze Lucille Wiss Liliia Horbal Klaus Biemel Laure Asselin Lysiane Richert Source Type: research
Fragrances as a trigger of immune responses in different environments
Toxicol In Vitro. 2023 Dec 22:105769. doi: 10.1016/j.tiv.2023.105769. Online ahead of print.ABSTRACTFragrances can cause allergic skin reactions, expressed as allergic contact dermatitis and reactions in the respiratory tract that range from acute temporary upper airway irritation to obstructive lung disease. These adverse health effects may result from the stimulation of a specific (adaptive) immune response. Th1 cells, which essentially produce interleukin-2 (IL-2) and interferon-γ (IFN-γ), play a key role in allergic contact dermatitis and also on allergic sensitization to common allergens (e.g., nickel and fragrance)...
Source: Toxicology in Vitro - December 24, 2023 Category: Toxicology Authors: M Macchione K Yoshizaki D P Frias K Maier J Smelan C M Prado T Mauad Source Type: research
Dinaciclib inhibits the growth of acute myeloid leukemia cells through either cell cycle-related or ERK1/STAT3/MYC pathways
Toxicol In Vitro. 2023 Dec 20:105768. doi: 10.1016/j.tiv.2023.105768. Online ahead of print.ABSTRACTAlthough immature differentiation and uncontrolled proliferation of hematopoietic stem cells are thought to be the primary mechanisms of acute myeloid leukemia (AML), the pathophysiology in most cases remains unclear. Dinaciclib, a selective small molecule targeting multiple cyclin-dependent kinases (CDKs), is currently being evaluated in oncological clinical trials. Despite the proven anticancer potential of dinaciclib, the differential molecular mechanisms by which it inhibits the growth of different AML cell lines remain ...
Source: Toxicology in Vitro - December 22, 2023 Category: Toxicology Authors: Chieh-Lin Jerry Teng Pang-Ting Cheng Yu-Chiao Cheng Jia-Rung Tsai Mei-Chih Chen Ho Lin Source Type: research
Dinaciclib inhibits the growth of acute myeloid leukemia cells through either cell cycle-related or ERK1/STAT3/MYC pathways
Toxicol In Vitro. 2023 Dec 20:105768. doi: 10.1016/j.tiv.2023.105768. Online ahead of print.ABSTRACTAlthough immature differentiation and uncontrolled proliferation of hematopoietic stem cells are thought to be the primary mechanisms of acute myeloid leukemia (AML), the pathophysiology in most cases remains unclear. Dinaciclib, a selective small molecule targeting multiple cyclin-dependent kinases (CDKs), is currently being evaluated in oncological clinical trials. Despite the proven anticancer potential of dinaciclib, the differential molecular mechanisms by which it inhibits the growth of different AML cell lines remain ...
Source: Toxicology in Vitro - December 22, 2023 Category: Toxicology Authors: Chieh-Lin Jerry Teng Pang-Ting Cheng Yu-Chiao Cheng Jia-Rung Tsai Mei-Chih Chen Ho Lin Source Type: research
Delayed clearance of the pro-carcinogen benzo[a]pyrene in PLHC-1 cells when co-exposed to the antifungal drug clotrimazole and effects on the CYP1A biomarker
Toxicol In Vitro. 2023 Dec 18:105767. doi: 10.1016/j.tiv.2023.105767. Online ahead of print.ABSTRACTCytochrome P450 1 A (CYP1A) is a key enzyme in the metabolism of the polycyclic aromatic hydrocarbon (PAH) benzo[a]pyrene (BaP) in animals, and a biomarker for environmental PAH exposure. The common antimycotic imidazole drug clotrimazole (CLO) has been detected in the aquatic environment and likely co-exists with BaP. Like BaP, CLO can bind to CYP1A enzymes and can act as a CYP1A inhibitor. Co-exposure of BaP with CLO significantly delayed BaP elimination in a fish liver cell line (PLHC-1). Intracellular BaP concentration w...
Source: Toxicology in Vitro - December 20, 2023 Category: Toxicology Authors: Maja Edenius Anne Farbrot Anders Blom Malin C Celander Source Type: research
Delayed clearance of the pro-carcinogen benzo[a]pyrene in PLHC-1 cells when co-exposed to the antifungal drug clotrimazole and effects on the CYP1A biomarker
Toxicol In Vitro. 2023 Dec 18:105767. doi: 10.1016/j.tiv.2023.105767. Online ahead of print.ABSTRACTCytochrome P450 1 A (CYP1A) is a key enzyme in the metabolism of the polycyclic aromatic hydrocarbon (PAH) benzo[a]pyrene (BaP) in animals, and a biomarker for environmental PAH exposure. The common antimycotic imidazole drug clotrimazole (CLO) has been detected in the aquatic environment and likely co-exists with BaP. Like BaP, CLO can bind to CYP1A enzymes and can act as a CYP1A inhibitor. Co-exposure of BaP with CLO significantly delayed BaP elimination in a fish liver cell line (PLHC-1). Intracellular BaP concentration w...
Source: Toxicology in Vitro - December 20, 2023 Category: Toxicology Authors: Maja Edenius Anne Farbrot Anders Blom Malin C Celander Source Type: research
Delayed clearance of the pro-carcinogen benzo[a]pyrene in PLHC-1 cells when co-exposed to the antifungal drug clotrimazole and effects on the CYP1A biomarker
Toxicol In Vitro. 2023 Dec 18:105767. doi: 10.1016/j.tiv.2023.105767. Online ahead of print.ABSTRACTCytochrome P450 1 A (CYP1A) is a key enzyme in the metabolism of the polycyclic aromatic hydrocarbon (PAH) benzo[a]pyrene (BaP) in animals, and a biomarker for environmental PAH exposure. The common antimycotic imidazole drug clotrimazole (CLO) has been detected in the aquatic environment and likely co-exists with BaP. Like BaP, CLO can bind to CYP1A enzymes and can act as a CYP1A inhibitor. Co-exposure of BaP with CLO significantly delayed BaP elimination in a fish liver cell line (PLHC-1). Intracellular BaP concentration w...
Source: Toxicology in Vitro - December 20, 2023 Category: Toxicology Authors: Maja Edenius Anne Farbrot Anders Blom Malin C Celander Source Type: research
Reduced toxic effects of nano ‑copper sulfate in comparison of bulk CuSO < sub > 4 < /sub > on biochemical parameters in the Rohu (Labeo rohita)
Toxicol In Vitro. 2023 Dec 15:105766. doi: 10.1016/j.tiv.2023.105766. Online ahead of print.ABSTRACTConsidering the wide application of nanoparticles in various fields of life and growing concern regarding their toxic effects, the present study was designed with the aim to evaluate the potential risks of using copper sulfate nanoparticles (CuSO4-NPs) in comparison to bulk form. Nanoparticles of CuSO4, having mean size of 73 nm were prepared by ball milling method, and fingerlings of Labeo rohita were exposed to two levels, 20 and 100 μg L-1 of CuSO4 in both bulk and nano forms for 28 days and their comparative effects on ...
Source: Toxicology in Vitro - December 17, 2023 Category: Toxicology Authors: Azka Anwar Fahim Ullah Khan Waqar Younas Muhib Zaman Muhammad Noorullah Li'ang Li Amina Zuberi Youji Wang Source Type: research
Reduced toxic effects of nano ‑copper sulfate in comparison of bulk CuSO < sub > 4 < /sub > on biochemical parameters in the Rohu (Labeo rohita)
Toxicol In Vitro. 2023 Dec 15:105766. doi: 10.1016/j.tiv.2023.105766. Online ahead of print.ABSTRACTConsidering the wide application of nanoparticles in various fields of life and growing concern regarding their toxic effects, the present study was designed with the aim to evaluate the potential risks of using copper sulfate nanoparticles (CuSO4-NPs) in comparison to bulk form. Nanoparticles of CuSO4, having mean size of 73 nm were prepared by ball milling method, and fingerlings of Labeo rohita were exposed to two levels, 20 and 100 μg L-1 of CuSO4 in both bulk and nano forms for 28 days and their comparative effects on ...
Source: Toxicology in Vitro - December 17, 2023 Category: Toxicology Authors: Azka Anwar Fahim Ullah Khan Waqar Younas Muhib Zaman Muhammad Noorullah Li'ang Li Amina Zuberi Youji Wang Source Type: research
Reduced toxic effects of nano ‑copper sulfate in comparison of bulk CuSO < sub > 4 < /sub > on biochemical parameters in the Rohu (Labeo rohita)
Toxicol In Vitro. 2023 Dec 15:105766. doi: 10.1016/j.tiv.2023.105766. Online ahead of print.ABSTRACTConsidering the wide application of nanoparticles in various fields of life and growing concern regarding their toxic effects, the present study was designed with the aim to evaluate the potential risks of using copper sulfate nanoparticles (CuSO4-NPs) in comparison to bulk form. Nanoparticles of CuSO4, having mean size of 73 nm were prepared by ball milling method, and fingerlings of Labeo rohita were exposed to two levels, 20 and 100 μg L-1 of CuSO4 in both bulk and nano forms for 28 days and their comparative effects on ...
Source: Toxicology in Vitro - December 17, 2023 Category: Toxicology Authors: Azka Anwar Fahim Ullah Khan Waqar Younas Muhib Zaman Muhammad Noorullah Li'ang Li Amina Zuberi Youji Wang Source Type: research
Synthesis of homogeneous spherical selenium nanoparticles through a chemical method for cancer therapy applications
Toxicol In Vitro. 2023 Dec 14:105765. doi: 10.1016/j.tiv.2023.105765. Online ahead of print.ABSTRACTOxidative stress is associated with pathologies affecting various organs or metabolic pathways. Thus, targeting oxidative stress might represent a valid therapeutic option. Selenium nanoparticles (SeNPs) are reported to exert antioxidant effects by many mechanisms. Our purpose was to assess in vitro on normal (MRC-5) and cancer (PANC-1) cell lines the potential of SeNPs for inducing cytotoxicity and redox modulation. They were synthesized through a chemogenic method and characterized through advanced microscopy techniques. S...
Source: Toxicology in Vitro - December 16, 2023 Category: Toxicology Authors: E I Adam-Dima M Bala ș M Anastasescu C Purdel D Margin ă Source Type: research
Organochlorine pesticides disrupt T helper cell regulation and reduce IL-2 and IFN- γ favoring infection and production of autoantibodies among pemphigus patients
This study investigated the effect of environmental factors like organochlorine pesticides (OCPs) on proportions of different T lymphocyte subsets and their cytokine secretion in-vitro among pemphigus patients, before and after specific immunosuppressive therapy. Higher levels of OCPs like β-HCH (isoform of hexachlorohexane), α-endosulfan (a form of endosulfan) and p,p΄-DDE (a metabolite of o,p'-dichlorodiphenyltrichloroethane) were observed in the blood of pemphigus patients as compared to healthy controls. HCH and DDT exposure caused specific reduction in CD8+CD45RA+ and CD4+CD25+ T lymphocyte subpopulations in these ...
Source: Toxicology in Vitro - December 15, 2023 Category: Toxicology Authors: Sajad Ahmad Dar Shukla Das Mohammed Ageeli Hakami Naseem Akhter Mohammad Mustafa Arshad Jawed Sambit Nath Bhattacharya Basu Dev Banerjee Abrar Ahmad Source Type: research
