In vitro test methods for evaluating high molecular weight polyethylene oxide polymer induced hemolytic and thrombotic potential
Toxicol In Vitro. 2024 Feb 22:105793. doi: 10.1016/j.tiv.2024.105793. Online ahead of print.ABSTRACTTo combat opioid abuse, the U.S. Food and Drug Administration (FDA) released a comprehensive action plan to address opioid addiction, abuse, and overdose that included increasing the prevalence of abuse-deterrent formulations (ADFs) in opioid tablets. Polyethylene oxide (PEO) has been widely used as an excipient to deter abuse via nasal insufflation. However, changes in abuse patterns have led to unexpected shifts in abuse from the nasal route to intravenous injection. Case reports identify adverse effects similar to thrombo...
Source: Toxicology in Vitro - February 24, 2024 Category: Toxicology Authors: Dongjune Kim Rucha Natu Richard Malinauskas Jin Hyen Baek Paul Buehler Xin Feng Haiou Qu Julia Pinto Xiaoming Xu Luke Herbertson Source Type: research
Hsa_circ_0003356 suppresses gastric cancer progression via miR-556-5p/FKBP5 axis
CONCLUSION: Circ_0003356 is identified as a potential biomarker of the prognosis of human gastric cancer, and circ_0003356/miR-556-5p/FKBP5 axis could be a promising target in gastric cancer treatment.PMID:38401744 | DOI:10.1016/j.tiv.2024.105787 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - February 24, 2024 Category: Toxicology Authors: Chuanhui Lu Jing Wu Xiaoguang Li Wei Huang Yongmu Fang Ying Huang Source Type: research
In vitro test methods for evaluating high molecular weight polyethylene oxide polymer induced hemolytic and thrombotic potential
Toxicol In Vitro. 2024 Feb 22:105793. doi: 10.1016/j.tiv.2024.105793. Online ahead of print.ABSTRACTTo combat opioid abuse, the U.S. Food and Drug Administration (FDA) released a comprehensive action plan to address opioid addiction, abuse, and overdose that included increasing the prevalence of abuse-deterrent formulations (ADFs) in opioid tablets. Polyethylene oxide (PEO) has been widely used as an excipient to deter abuse via nasal insufflation. However, changes in abuse patterns have led to unexpected shifts in abuse from the nasal route to intravenous injection. Case reports identify adverse effects similar to thrombo...
Source: Toxicology in Vitro - February 24, 2024 Category: Toxicology Authors: Dongjune Kim Rucha Natu Richard Malinauskas Jin Hyen Baek Paul Buehler Xin Feng Haiou Qu Julia Pinto Xiaoming Xu Luke Herbertson Source Type: research
A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards
In this study, we compare in vitro and ex vivo cell models that represent dermal exposures (including HaCaT cells, primary keratinocytes, and reconstructed human epidermis (RhE)), and when possible consider these in the context of regulatory accepted OECD TG for in vitro dermal irritation. Various benchmark nanomaterials were used to assess markers of cell stress in each cell model. In addition, we evaluated different dosing strategies that have been used when applying the OECD TG for dermal irritation in assessment of nanomaterials, and how inconsistencies in the approach used can have considerable impact of the conclusio...
Source: Toxicology in Vitro - February 16, 2024 Category: Toxicology Authors: Polly McLean Jessica Marshall Amaia Garc ía-Bilbao David Beal Alberto Katsumiti Marie Carri ère Matthew S P Boyles Source Type: research
A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards
In this study, we compare in vitro and ex vivo cell models that represent dermal exposures (including HaCaT cells, primary keratinocytes, and reconstructed human epidermis (RhE)), and when possible consider these in the context of regulatory accepted OECD TG for in vitro dermal irritation. Various benchmark nanomaterials were used to assess markers of cell stress in each cell model. In addition, we evaluated different dosing strategies that have been used when applying the OECD TG for dermal irritation in assessment of nanomaterials, and how inconsistencies in the approach used can have considerable impact of the conclusio...
Source: Toxicology in Vitro - February 16, 2024 Category: Toxicology Authors: Polly McLean Jessica Marshall Amaia Garc ía David Beal Alberto Katsumiti Marie Carri ère Matthew Boyles Source Type: research
A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards
In this study, we compare in vitro and ex vivo cell models that represent dermal exposures (including HaCaT cells, primary keratinocytes, and reconstructed human epidermis (RhE)), and when possible consider these in the context of regulatory accepted OECD TG for in vitro dermal irritation. Various benchmark nanomaterials were used to assess markers of cell stress in each cell model. In addition, we evaluated different dosing strategies that have been used when applying the OECD TG for dermal irritation in assessment of nanomaterials, and how inconsistencies in the approach used can have considerable impact of the conclusio...
Source: Toxicology in Vitro - February 16, 2024 Category: Toxicology Authors: Polly McLean Jessica Marshall Amaia Garc ía David Beal Alberto Katsumiti Marie Carri ère Matthew Boyles Source Type: research
A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards
In this study, we compare in vitro and ex vivo cell models that represent dermal exposures (including HaCaT cells, primary keratinocytes, and reconstructed human epidermis (RhE)), and when possible consider these in the context of regulatory accepted OECD TG for in vitro dermal irritation. Various benchmark nanomaterials were used to assess markers of cell stress in each cell model. In addition, we evaluated different dosing strategies that have been used when applying the OECD TG for dermal irritation in assessment of nanomaterials, and how inconsistencies in the approach used can have considerable impact of the conclusio...
Source: Toxicology in Vitro - February 16, 2024 Category: Toxicology Authors: Polly McLean Jessica Marshall Amaia Garc ía David Beal Alberto Katsumiti Marie Carri ère Matthew Boyles Source Type: research
A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards
In this study, we compare in vitro and ex vivo cell models that represent dermal exposures (including HaCaT cells, primary keratinocytes, and reconstructed human epidermis (RhE)), and when possible consider these in the context of regulatory accepted OECD TG for in vitro dermal irritation. Various benchmark nanomaterials were used to assess markers of cell stress in each cell model. In addition, we evaluated different dosing strategies that have been used when applying the OECD TG for dermal irritation in assessment of nanomaterials, and how inconsistencies in the approach used can have considerable impact of the conclusio...
Source: Toxicology in Vitro - February 16, 2024 Category: Toxicology Authors: Polly McLean Jessica Marshall Amaia Garc ía David Beal Alberto Katsumiti Marie Carri ère Matthew Boyles Source Type: research
A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards
In this study, we compare in vitro and ex vivo cell models that represent dermal exposures (including HaCaT cells, primary keratinocytes, and reconstructed human epidermis (RhE)), and when possible consider these in the context of regulatory accepted OECD TG for in vitro dermal irritation. Various benchmark nanomaterials were used to assess markers of cell stress in each cell model. In addition, we evaluated different dosing strategies that have been used when applying the OECD TG for dermal irritation in assessment of nanomaterials, and how inconsistencies in the approach used can have considerable impact of the conclusio...
Source: Toxicology in Vitro - February 16, 2024 Category: Toxicology Authors: Polly McLean Jessica Marshall Amaia Garc ía David Beal Alberto Katsumiti Marie Carri ère Matthew Boyles Source Type: research
A comparison of dermal toxicity models; assessing suitability for safe(r)-by-design decision-making and for screening nanomaterial hazards
In this study, we compare in vitro and ex vivo cell models that represent dermal exposures (including HaCaT cells, primary keratinocytes, and reconstructed human epidermis (RhE)), and when possible consider these in the context of regulatory accepted OECD TG for in vitro dermal irritation. Various benchmark nanomaterials were used to assess markers of cell stress in each cell model. In addition, we evaluated different dosing strategies that have been used when applying the OECD TG for dermal irritation in assessment of nanomaterials, and how inconsistencies in the approach used can have considerable impact of the conclusio...
Source: Toxicology in Vitro - February 16, 2024 Category: Toxicology Authors: Polly McLean Jessica Marshall Amaia Garc ía David Beal Alberto Katsumiti Marie Carri ère Matthew Boyles Source Type: research
Gill epithelial cell line ASG-10 from Atlantic salmon as a new research tool for solving water quality challenges in aquaculture
In conclusion, the ASG-10 cell line is a promising in vitro model to study water quality challenges and whole water samples.PMID:38355023 | DOI:10.1016/j.tiv.2024.105790 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - February 14, 2024 Category: Toxicology Authors: Anita Solhaug P ål A Olsvik Prabhugouda Siriyappagouder Randi Faller Torstein Kristensen Source Type: research
Development of natural cosmetic emulsion using the by-product of Lecythis pisonis seed
Toxicol In Vitro. 2024 Feb 12:105791. doi: 10.1016/j.tiv.2024.105791. Online ahead of print.ABSTRACTNatural products and their biological activities are currently a subject of great interest to the industrial and scientific sector, due to society's awareness of the proper use of biodiversity and economic and sustainability. To promote the sustainable use of biomass the extract of the by-product of the shell seed of Lecythis pisonis was applied to develop a natural cosmetic emulsion. To ensure safety for its topical use the cytotoxic activity of its crude extract was evaluated by the colorimetric method of 3- bromide (4,5-d...
Source: Toxicology in Vitro - February 14, 2024 Category: Toxicology Authors: Danielle Braga Portes Lucas Wetler Abeldt Cristiane Dos Santos Giubert Denise Coutinho Endringer Elis ângela Flávia Pimentel Source Type: research
Gill epithelial cell line ASG-10 from Atlantic salmon as a new research tool for solving water quality challenges in aquaculture
In conclusion, the ASG-10 cell line is a promising in vitro model to study water quality challenges and whole water samples.PMID:38355023 | DOI:10.1016/j.tiv.2024.105790 (Source: Toxicology in Vitro)
Source: Toxicology in Vitro - February 14, 2024 Category: Toxicology Authors: Anita Solhaug P ål A Olsvik Prabhugouda Siriyappagouder Randi Faller Torstein Kristensen Source Type: research
Development of natural cosmetic emulsion using the by-product of Lecythis pisonis seed
Toxicol In Vitro. 2024 Feb 12:105791. doi: 10.1016/j.tiv.2024.105791. Online ahead of print.ABSTRACTNatural products and their biological activities are currently a subject of great interest to the industrial and scientific sector, due to society's awareness of the proper use of biodiversity and economic and sustainability. To promote the sustainable use of biomass the extract of the by-product of the shell seed of Lecythis pisonis was applied to develop a natural cosmetic emulsion. To ensure safety for its topical use the cytotoxic activity of its crude extract was evaluated by the colorimetric method of 3- bromide (4,5-d...
Source: Toxicology in Vitro - February 14, 2024 Category: Toxicology Authors: Danielle Braga Portes Lucas Wetler Abeldt Cristiane Dos Santos Giubert Denise Coutinho Endringer Elis ângela Flávia Pimentel Source Type: research
Interaction of mycotoxins zearalenone, α-zearalenol, and β-zearalenol with cytochrome P450 (CYP1A2, 2C9, 2C19, 2D6, and 3A4) enzymes and organic anion transporting polypeptides (OATP1A2, OATP1B1, OATP1B3, and OATP2B1)
Toxicol In Vitro. 2024 Feb 8;96:105789. doi: 10.1016/j.tiv.2024.105789. Online ahead of print.ABSTRACTZearalenone (ZEN) is a mycoestrogen produced by Fusarium fungi. ZEN is a frequent contaminant in cereal-based products, representing significant health threat. The major reduced metabolites of ZEN are α-zearalenol (α-ZEL) and β-zearalenol (β-ZEL). Since the toxicokinetic interactions of ZEN/ZELs with cytochrome P450 enzymes (CYPs) and organic anion transporting polypeptides (OATPs) have been barely characterized, we examined these interactions applying in vitro models. ZEN and ZELs were relatively strong inhibitors of ...
Source: Toxicology in Vitro - February 10, 2024 Category: Toxicology Authors: Hana Kaci Ágnes Dombi Patrik G ömbös Andr ás Szabó Éva Bakos Csilla Özvegy-Laczka Mikl ós Poór Source Type: research
