Differential protein-protein interactions underlie signaling mediated by the TCR and a 4-1BB domain-containing CAR
Sci Signal. 2024 Mar 5;17(826):eadd4671. doi: 10.1126/scisignal.add4671. Epub 2024 Mar 5.ABSTRACTCells rely on activity-dependent protein-protein interactions to convey biological signals. For chimeric antigen receptor (CAR) T cells containing a 4-1BB costimulatory domain, receptor engagement is thought to stimulate the formation of protein complexes similar to those stimulated by T cell receptor (TCR)-mediated signaling, but the number and type of protein interaction-mediating binding domains differ between CARs and TCRs. Here, we performed coimmunoprecipitation mass spectrometry analysis of a second-generation, CD19-dire...
Source: Science Signaling - March 5, 2024 Category: Biomedical Science Authors: Samuel A Ritmeester-Loy Isabella H Draper Eric C Bueter Jonathan D Lautz Yue Zhang-Wong Joshua A Gustafson Ashley L Wilson Chenwei Lin Philip R Gafken Michael C Jensen Rimas Orentas Stephen E P Smith Source Type: research
eEF1A2 promotes PTEN-GSK3 β-SCF complex-dependent degradation of Aurora kinase A and is inactivated in breast cancer
Sci Signal. 2024 Mar 5;17(826):eadh4475. doi: 10.1126/scisignal.adh4475. Epub 2024 Mar 5.ABSTRACTThe translation elongation factor eEF1A promotes protein synthesis. Its methylation by METTL13 increases its activity, supporting tumor growth. However, in some cancers, a high abundance of eEF1A isoforms is associated with a good prognosis. Here, we found that eEF1A2 exhibited oncogenic or tumor-suppressor functions depending on its interaction with METTL13 or the phosphatase PTEN, respectively. METTL13 and PTEN competed for interaction with eEF1A2 in the same structural domain. PTEN-bound eEF1A2 promoted the ubiquitination an...
Source: Science Signaling - March 5, 2024 Category: Biomedical Science Authors: Warapen Treekitkarnmongkol Luisa M Solis Deivendran Sankaran Mihai Gagea Pankaj K Singh Ragini Mistry Tristian Nguyen Kazuharu Kai Jiajun Liu Kaori Sasai Yoshimi Jitsumori Jianwen Liu Norio Nagao Fabio Stossi Michael A Mancini Ignacio I Wistuba Alastair M Source Type: research
Bring in the B team
Sci Signal. 2024 Mar 5;17(826):eado9536. doi: 10.1126/scisignal.ado9536. Epub 2024 Mar 5.ABSTRACTATP released by osteoblasts signals through the purinergic receptor P2RX4 to optimize plasma cell survival.PMID:38442202 | DOI:10.1126/scisignal.ado9536 (Source: Science Signaling)
Source: Science Signaling - March 5, 2024 Category: Biomedical Science Authors: Amy E Baek Source Type: research
Differential protein-protein interactions underlie signaling mediated by the TCR and a 4-1BB domain-containing CAR
Sci Signal. 2024 Mar 5;17(826):eadd4671. doi: 10.1126/scisignal.add4671. Epub 2024 Mar 5.ABSTRACTCells rely on activity-dependent protein-protein interactions to convey biological signals. For chimeric antigen receptor (CAR) T cells containing a 4-1BB costimulatory domain, receptor engagement is thought to stimulate the formation of protein complexes similar to those stimulated by T cell receptor (TCR)-mediated signaling, but the number and type of protein interaction-mediating binding domains differ between CARs and TCRs. Here, we performed coimmunoprecipitation mass spectrometry analysis of a second-generation, CD19-dire...
Source: Science Signaling - March 5, 2024 Category: Biomedical Science Authors: Samuel A Ritmeester-Loy Isabella H Draper Eric C Bueter Jonathan D Lautz Yue Zhang-Wong Joshua A Gustafson Ashley L Wilson Chenwei Lin Philip R Gafken Michael C Jensen Rimas Orentas Stephen E P Smith Source Type: research
eEF1A2 promotes PTEN-GSK3 β-SCF complex-dependent degradation of Aurora kinase A and is inactivated in breast cancer
Sci Signal. 2024 Mar 5;17(826):eadh4475. doi: 10.1126/scisignal.adh4475. Epub 2024 Mar 5.ABSTRACTThe translation elongation factor eEF1A promotes protein synthesis. Its methylation by METTL13 increases its activity, supporting tumor growth. However, in some cancers, a high abundance of eEF1A isoforms is associated with a good prognosis. Here, we found that eEF1A2 exhibited oncogenic or tumor-suppressor functions depending on its interaction with METTL13 or the phosphatase PTEN, respectively. METTL13 and PTEN competed for interaction with eEF1A2 in the same structural domain. PTEN-bound eEF1A2 promoted the ubiquitination an...
Source: Science Signaling - March 5, 2024 Category: Biomedical Science Authors: Warapen Treekitkarnmongkol Luisa M Solis Deivendran Sankaran Mihai Gagea Pankaj K Singh Ragini Mistry Tristian Nguyen Kazuharu Kai Jiajun Liu Kaori Sasai Yoshimi Jitsumori Jianwen Liu Norio Nagao Fabio Stossi Michael A Mancini Ignacio I Wistuba Alastair M Source Type: research
Bring in the B team
Sci Signal. 2024 Mar 5;17(826):eado9536. doi: 10.1126/scisignal.ado9536. Epub 2024 Mar 5.ABSTRACTATP released by osteoblasts signals through the purinergic receptor P2RX4 to optimize plasma cell survival.PMID:38442202 | DOI:10.1126/scisignal.ado9536 (Source: Science Signaling)
Source: Science Signaling - March 5, 2024 Category: Biomedical Science Authors: Amy E Baek Source Type: research
Differential protein-protein interactions underlie signaling mediated by the TCR and a 4-1BB domain-containing CAR
Sci Signal. 2024 Mar 5;17(826):eadd4671. doi: 10.1126/scisignal.add4671. Epub 2024 Mar 5.ABSTRACTCells rely on activity-dependent protein-protein interactions to convey biological signals. For chimeric antigen receptor (CAR) T cells containing a 4-1BB costimulatory domain, receptor engagement is thought to stimulate the formation of protein complexes similar to those stimulated by T cell receptor (TCR)-mediated signaling, but the number and type of protein interaction-mediating binding domains differ between CARs and TCRs. Here, we performed coimmunoprecipitation mass spectrometry analysis of a second-generation, CD19-dire...
Source: Science Signaling - March 5, 2024 Category: Biomedical Science Authors: Samuel A Ritmeester-Loy Isabella H Draper Eric C Bueter Jonathan D Lautz Yue Zhang-Wong Joshua A Gustafson Ashley L Wilson Chenwei Lin Philip R Gafken Michael C Jensen Rimas Orentas Stephen E P Smith Source Type: research
eEF1A2 promotes PTEN-GSK3 β-SCF complex-dependent degradation of Aurora kinase A and is inactivated in breast cancer
Sci Signal. 2024 Mar 5;17(826):eadh4475. doi: 10.1126/scisignal.adh4475. Epub 2024 Mar 5.ABSTRACTThe translation elongation factor eEF1A promotes protein synthesis. Its methylation by METTL13 increases its activity, supporting tumor growth. However, in some cancers, a high abundance of eEF1A isoforms is associated with a good prognosis. Here, we found that eEF1A2 exhibited oncogenic or tumor-suppressor functions depending on its interaction with METTL13 or the phosphatase PTEN, respectively. METTL13 and PTEN competed for interaction with eEF1A2 in the same structural domain. PTEN-bound eEF1A2 promoted the ubiquitination an...
Source: Science Signaling - March 5, 2024 Category: Biomedical Science Authors: Warapen Treekitkarnmongkol Luisa M Solis Deivendran Sankaran Mihai Gagea Pankaj K Singh Ragini Mistry Tristian Nguyen Kazuharu Kai Jiajun Liu Kaori Sasai Yoshimi Jitsumori Jianwen Liu Norio Nagao Fabio Stossi Michael A Mancini Ignacio I Wistuba Alastair M Source Type: research
Bring in the B team
Sci Signal. 2024 Mar 5;17(826):eado9536. doi: 10.1126/scisignal.ado9536. Epub 2024 Mar 5.ABSTRACTATP released by osteoblasts signals through the purinergic receptor P2RX4 to optimize plasma cell survival.PMID:38442202 | DOI:10.1126/scisignal.ado9536 (Source: Science Signaling)
Source: Science Signaling - March 5, 2024 Category: Biomedical Science Authors: Amy E Baek Source Type: research
Differential protein-protein interactions underlie signaling mediated by the TCR and a 4-1BB domain-containing CAR
Sci Signal. 2024 Mar 5;17(826):eadd4671. doi: 10.1126/scisignal.add4671. Epub 2024 Mar 5.ABSTRACTCells rely on activity-dependent protein-protein interactions to convey biological signals. For chimeric antigen receptor (CAR) T cells containing a 4-1BB costimulatory domain, receptor engagement is thought to stimulate the formation of protein complexes similar to those stimulated by T cell receptor (TCR)-mediated signaling, but the number and type of protein interaction-mediating binding domains differ between CARs and TCRs. Here, we performed coimmunoprecipitation mass spectrometry analysis of a second-generation, CD19-dire...
Source: Science Signaling - March 5, 2024 Category: Biomedical Science Authors: Samuel A Ritmeester-Loy Isabella H Draper Eric C Bueter Jonathan D Lautz Yue Zhang-Wong Joshua A Gustafson Ashley L Wilson Chenwei Lin Philip R Gafken Michael C Jensen Rimas Orentas Stephen E P Smith Source Type: research
eEF1A2 promotes PTEN-GSK3 β-SCF complex-dependent degradation of Aurora kinase A and is inactivated in breast cancer
Sci Signal. 2024 Mar 5;17(826):eadh4475. doi: 10.1126/scisignal.adh4475. Epub 2024 Mar 5.ABSTRACTThe translation elongation factor eEF1A promotes protein synthesis. Its methylation by METTL13 increases its activity, supporting tumor growth. However, in some cancers, a high abundance of eEF1A isoforms is associated with a good prognosis. Here, we found that eEF1A2 exhibited oncogenic or tumor-suppressor functions depending on its interaction with METTL13 or the phosphatase PTEN, respectively. METTL13 and PTEN competed for interaction with eEF1A2 in the same structural domain. PTEN-bound eEF1A2 promoted the ubiquitination an...
Source: Science Signaling - March 5, 2024 Category: Biomedical Science Authors: Warapen Treekitkarnmongkol Luisa M Solis Deivendran Sankaran Mihai Gagea Pankaj K Singh Ragini Mistry Tristian Nguyen Kazuharu Kai Jiajun Liu Kaori Sasai Yoshimi Jitsumori Jianwen Liu Norio Nagao Fabio Stossi Michael A Mancini Ignacio I Wistuba Alastair M Source Type: research
Bring in the B team
Sci Signal. 2024 Mar 5;17(826):eado9536. doi: 10.1126/scisignal.ado9536. Epub 2024 Mar 5.ABSTRACTATP released by osteoblasts signals through the purinergic receptor P2RX4 to optimize plasma cell survival.PMID:38442202 | DOI:10.1126/scisignal.ado9536 (Source: Science Signaling)
Source: Science Signaling - March 5, 2024 Category: Biomedical Science Authors: Amy E Baek Source Type: research
Differential protein-protein interactions underlie signaling mediated by the TCR and a 4-1BB domain-containing CAR
Sci Signal. 2024 Mar 5;17(826):eadd4671. doi: 10.1126/scisignal.add4671. Epub 2024 Mar 5.ABSTRACTCells rely on activity-dependent protein-protein interactions to convey biological signals. For chimeric antigen receptor (CAR) T cells containing a 4-1BB costimulatory domain, receptor engagement is thought to stimulate the formation of protein complexes similar to those stimulated by T cell receptor (TCR)-mediated signaling, but the number and type of protein interaction-mediating binding domains differ between CARs and TCRs. Here, we performed coimmunoprecipitation mass spectrometry analysis of a second-generation, CD19-dire...
Source: Science Signaling - March 5, 2024 Category: Biomedical Science Authors: Samuel A Ritmeester-Loy Isabella H Draper Eric C Bueter Jonathan D Lautz Yue Zhang-Wong Joshua A Gustafson Ashley L Wilson Chenwei Lin Philip R Gafken Michael C Jensen Rimas Orentas Stephen E P Smith Source Type: research
eEF1A2 promotes PTEN-GSK3 β-SCF complex-dependent degradation of Aurora kinase A and is inactivated in breast cancer
Sci Signal. 2024 Mar 5;17(826):eadh4475. doi: 10.1126/scisignal.adh4475. Epub 2024 Mar 5.ABSTRACTThe translation elongation factor eEF1A promotes protein synthesis. Its methylation by METTL13 increases its activity, supporting tumor growth. However, in some cancers, a high abundance of eEF1A isoforms is associated with a good prognosis. Here, we found that eEF1A2 exhibited oncogenic or tumor-suppressor functions depending on its interaction with METTL13 or the phosphatase PTEN, respectively. METTL13 and PTEN competed for interaction with eEF1A2 in the same structural domain. PTEN-bound eEF1A2 promoted the ubiquitination an...
Source: Science Signaling - March 5, 2024 Category: Biomedical Science Authors: Warapen Treekitkarnmongkol Luisa M Solis Deivendran Sankaran Mihai Gagea Pankaj K Singh Ragini Mistry Tristian Nguyen Kazuharu Kai Jiajun Liu Kaori Sasai Yoshimi Jitsumori Jianwen Liu Norio Nagao Fabio Stossi Michael A Mancini Ignacio I Wistuba Alastair M Source Type: research
Bring in the B team
Sci Signal. 2024 Mar 5;17(826):eado9536. doi: 10.1126/scisignal.ado9536. Epub 2024 Mar 5.ABSTRACTATP released by osteoblasts signals through the purinergic receptor P2RX4 to optimize plasma cell survival.PMID:38442202 | DOI:10.1126/scisignal.ado9536 (Source: Science Signaling)
Source: Science Signaling - March 5, 2024 Category: Biomedical Science Authors: Amy E Baek Source Type: research
