Making the switch: The role of Gq in driving GRK selectivity at GPCRs
Sci Signal. 2022 Mar 22;15(726):eabo4949. doi: 10.1126/scisignal.abo4949. Epub 2022 Mar 22.ABSTRACTSelective engagement of signal transducers such as G proteins and β-arrestins with GPCRs upon stimulation with biased agonists is thought to be due to distinct receptor conformations. Kawakami et al. propose an additional mechanism whereby activation of Gq determines GPCR kinase subtype selectivity to the activated angiotensin receptor, leading to distinct binding modalities of β-arrestins and functional outcomes.PMID:35316098 | DOI:10.1126/scisignal.abo4949 (Source: Science Signaling)
Source: Science Signaling - March 22, 2022 Category: Biomedical Science Authors: Parishmita Sarma Shirsha Saha Arun K Shukla Source Type: research

Biased agonists of the chemokine receptor CXCR3 differentially signal through G α < sub > i < /sub > : β-arrestin complexes
Sci Signal. 2022 Mar 22;15(726):eabg5203. doi: 10.1126/scisignal.abg5203. Epub 2022 Mar 22.ABSTRACTG protein-coupled receptors (GPCRs) are the largest family of cell surface receptors and signal through the proximal effectors, G proteins and β-arrestins, to influence nearly every biological process. The G protein and β-arrestin signaling pathways have largely been considered separable; however, direct interactions between Gα proteins and β-arrestins have been described that appear to be part of a distinct GPCR signaling pathway. Within these complexes, Gαi/o, but not other Gα protein subtypes, directly interacts with...
Source: Science Signaling - March 22, 2022 Category: Biomedical Science Authors: Kevin Zheng Jeffrey S Smith Dylan S Eiger Anmol Warman Issac Choi Christopher C Honeycutt Noelia Boldizsar Jaimee N Gundry Thomas F Pack Asuka Inoue Marc G Caron Sudarshan Rajagopal Source Type: research

Compartmentalized prions
Sci Signal. 2022 Mar 22;15(726):eabq0860. doi: 10.1126/scisignal.abq0860. Epub 2022 Mar 22.ABSTRACTThe restriction of the yeast prion-like protein Whi3 to particular regions of the ER prevents its transmission to daughter cells.PMID:35316096 | DOI:10.1126/scisignal.abq0860 (Source: Science Signaling)
Source: Science Signaling - March 22, 2022 Category: Biomedical Science Authors: Annalisa M VanHook Source Type: research

The glucocorticoid receptor associates with RAS complexes to inhibit cell proliferation and tumor growth
Sci Signal. 2022 Mar 22;15(726):eabm4452. doi: 10.1126/scisignal.abm4452. Epub 2022 Mar 22.ABSTRACTMutations that activate members of the RAS family of GTPases are associated with various cancers and drive tumor growth. The glucocorticoid receptor (GR), a member of the nuclear receptor family, has been proposed to interact with and inhibit the activation of components of the PI3K-AKT and MAPK pathways downstream of RAS. In the absence of activating ligands, we found that GR was present in cytoplasmic KRAS-containing complexes and inhibited the activation of wild-type and oncogenic KRAS in mouse embryonic fibroblasts and hu...
Source: Science Signaling - March 22, 2022 Category: Biomedical Science Authors: Bozhena Caratti Miray Fidan Giorgio Caratti Kristina Breitenecker Melanie Engler Naser Kazemitash Rebecca Traut Rainer Wittig Emilio Casanova Mohammad Reza Ahmadian Jan P Tuckermann Herwig P Moll Ion Cristian Cirstea Source Type: research

Making the switch: The role of Gq in driving GRK selectivity at GPCRs
Sci Signal. 2022 Mar 22;15(726):eabo4949. doi: 10.1126/scisignal.abo4949. Epub 2022 Mar 22.ABSTRACTSelective engagement of signal transducers such as G proteins and β-arrestins with GPCRs upon stimulation with biased agonists is thought to be due to distinct receptor conformations. Kawakami et al. propose an additional mechanism whereby activation of Gq determines GPCR kinase subtype selectivity to the activated angiotensin receptor, leading to distinct binding modalities of β-arrestins and functional outcomes.PMID:35316098 | DOI:10.1126/scisignal.abo4949 (Source: Science Signaling)
Source: Science Signaling - March 22, 2022 Category: Biomedical Science Authors: Parishmita Sarma Shirsha Saha Arun K Shukla Source Type: research

Biased agonists of the chemokine receptor CXCR3 differentially signal through G α < sub > i < /sub > : β-arrestin complexes
Sci Signal. 2022 Mar 22;15(726):eabg5203. doi: 10.1126/scisignal.abg5203. Epub 2022 Mar 22.ABSTRACTG protein-coupled receptors (GPCRs) are the largest family of cell surface receptors and signal through the proximal effectors, G proteins and β-arrestins, to influence nearly every biological process. The G protein and β-arrestin signaling pathways have largely been considered separable; however, direct interactions between Gα proteins and β-arrestins have been described that appear to be part of a distinct GPCR signaling pathway. Within these complexes, Gαi/o, but not other Gα protein subtypes, directly interacts with...
Source: Science Signaling - March 22, 2022 Category: Biomedical Science Authors: Kevin Zheng Jeffrey S Smith Dylan S Eiger Anmol Warman Issac Choi Christopher C Honeycutt Noelia Boldizsar Jaimee N Gundry Thomas F Pack Asuka Inoue Marc G Caron Sudarshan Rajagopal Source Type: research

Compartmentalized prions
Sci Signal. 2022 Mar 22;15(726):eabq0860. doi: 10.1126/scisignal.abq0860. Epub 2022 Mar 22.ABSTRACTThe restriction of the yeast prion-like protein Whi3 to particular regions of the ER prevents its transmission to daughter cells.PMID:35316096 | DOI:10.1126/scisignal.abq0860 (Source: Science Signaling)
Source: Science Signaling - March 22, 2022 Category: Biomedical Science Authors: Annalisa M VanHook Source Type: research

The glucocorticoid receptor associates with RAS complexes to inhibit cell proliferation and tumor growth
Sci Signal. 2022 Mar 22;15(726):eabm4452. doi: 10.1126/scisignal.abm4452. Epub 2022 Mar 22.ABSTRACTMutations that activate members of the RAS family of GTPases are associated with various cancers and drive tumor growth. The glucocorticoid receptor (GR), a member of the nuclear receptor family, has been proposed to interact with and inhibit the activation of components of the PI3K-AKT and MAPK pathways downstream of RAS. In the absence of activating ligands, we found that GR was present in cytoplasmic KRAS-containing complexes and inhibited the activation of wild-type and oncogenic KRAS in mouse embryonic fibroblasts and hu...
Source: Science Signaling - March 22, 2022 Category: Biomedical Science Authors: Bozhena Caratti Miray Fidan Giorgio Caratti Kristina Breitenecker Melanie Engler Naser Kazemitash Rebecca Traut Rainer Wittig Emilio Casanova Mohammad Reza Ahmadian Jan P Tuckermann Herwig P Moll Ion Cristian Cirstea Source Type: research

Making the switch: The role of Gq in driving GRK selectivity at GPCRs
Sci Signal. 2022 Mar 22;15(726):eabo4949. doi: 10.1126/scisignal.abo4949. Epub 2022 Mar 22.ABSTRACTSelective engagement of signal transducers such as G proteins and β-arrestins with GPCRs upon stimulation with biased agonists is thought to be due to distinct receptor conformations. Kawakami et al. propose an additional mechanism whereby activation of Gq determines GPCR kinase subtype selectivity to the activated angiotensin receptor, leading to distinct binding modalities of β-arrestins and functional outcomes.PMID:35316098 | DOI:10.1126/scisignal.abo4949 (Source: Science Signaling)
Source: Science Signaling - March 22, 2022 Category: Biomedical Science Authors: Parishmita Sarma Shirsha Saha Arun K Shukla Source Type: research

Biased agonists of the chemokine receptor CXCR3 differentially signal through G α < sub > i < /sub > : β-arrestin complexes
Sci Signal. 2022 Mar 22;15(726):eabg5203. doi: 10.1126/scisignal.abg5203. Epub 2022 Mar 22.ABSTRACTG protein-coupled receptors (GPCRs) are the largest family of cell surface receptors and signal through the proximal effectors, G proteins and β-arrestins, to influence nearly every biological process. The G protein and β-arrestin signaling pathways have largely been considered separable; however, direct interactions between Gα proteins and β-arrestins have been described that appear to be part of a distinct GPCR signaling pathway. Within these complexes, Gαi/o, but not other Gα protein subtypes, directly interacts with...
Source: Science Signaling - March 22, 2022 Category: Biomedical Science Authors: Kevin Zheng Jeffrey S Smith Dylan S Eiger Anmol Warman Issac Choi Christopher C Honeycutt Noelia Boldizsar Jaimee N Gundry Thomas F Pack Asuka Inoue Marc G Caron Sudarshan Rajagopal Source Type: research

Compartmentalized prions
Sci Signal. 2022 Mar 22;15(726):eabq0860. doi: 10.1126/scisignal.abq0860. Epub 2022 Mar 22.ABSTRACTThe restriction of the yeast prion-like protein Whi3 to particular regions of the ER prevents its transmission to daughter cells.PMID:35316096 | DOI:10.1126/scisignal.abq0860 (Source: Science Signaling)
Source: Science Signaling - March 22, 2022 Category: Biomedical Science Authors: Annalisa M VanHook Source Type: research

The glucocorticoid receptor associates with RAS complexes to inhibit cell proliferation and tumor growth
Sci Signal. 2022 Mar 22;15(726):eabm4452. doi: 10.1126/scisignal.abm4452. Epub 2022 Mar 22.ABSTRACTMutations that activate members of the RAS family of GTPases are associated with various cancers and drive tumor growth. The glucocorticoid receptor (GR), a member of the nuclear receptor family, has been proposed to interact with and inhibit the activation of components of the PI3K-AKT and MAPK pathways downstream of RAS. In the absence of activating ligands, we found that GR was present in cytoplasmic KRAS-containing complexes and inhibited the activation of wild-type and oncogenic KRAS in mouse embryonic fibroblasts and hu...
Source: Science Signaling - March 22, 2022 Category: Biomedical Science Authors: Bozhena Caratti Miray Fidan Giorgio Caratti Kristina Breitenecker Melanie Engler Naser Kazemitash Rebecca Traut Rainer Wittig Emilio Casanova Mohammad Reza Ahmadian Jan P Tuckermann Herwig P Moll Ion Cristian Cirstea Source Type: research

Making the switch: The role of Gq in driving GRK selectivity at GPCRs
Sci Signal. 2022 Mar 22;15(726):eabo4949. doi: 10.1126/scisignal.abo4949. Epub 2022 Mar 22.ABSTRACTSelective engagement of signal transducers such as G proteins and β-arrestins with GPCRs upon stimulation with biased agonists is thought to be due to distinct receptor conformations. Kawakami et al. propose an additional mechanism whereby activation of Gq determines GPCR kinase subtype selectivity to the activated angiotensin receptor, leading to distinct binding modalities of β-arrestins and functional outcomes.PMID:35316098 | DOI:10.1126/scisignal.abo4949 (Source: Science Signaling)
Source: Science Signaling - March 22, 2022 Category: Biomedical Science Authors: Parishmita Sarma Shirsha Saha Arun K Shukla Source Type: research

Biased agonists of the chemokine receptor CXCR3 differentially signal through G α < sub > i < /sub > : β-arrestin complexes
Sci Signal. 2022 Mar 22;15(726):eabg5203. doi: 10.1126/scisignal.abg5203. Epub 2022 Mar 22.ABSTRACTG protein-coupled receptors (GPCRs) are the largest family of cell surface receptors and signal through the proximal effectors, G proteins and β-arrestins, to influence nearly every biological process. The G protein and β-arrestin signaling pathways have largely been considered separable; however, direct interactions between Gα proteins and β-arrestins have been described that appear to be part of a distinct GPCR signaling pathway. Within these complexes, Gαi/o, but not other Gα protein subtypes, directly interacts with...
Source: Science Signaling - March 22, 2022 Category: Biomedical Science Authors: Kevin Zheng Jeffrey S Smith Dylan S Eiger Anmol Warman Issac Choi Christopher C Honeycutt Noelia Boldizsar Jaimee N Gundry Thomas F Pack Asuka Inoue Marc G Caron Sudarshan Rajagopal Source Type: research

Compartmentalized prions
Sci Signal. 2022 Mar 22;15(726):eabq0860. doi: 10.1126/scisignal.abq0860. Epub 2022 Mar 22.ABSTRACTThe restriction of the yeast prion-like protein Whi3 to particular regions of the ER prevents its transmission to daughter cells.PMID:35316096 | DOI:10.1126/scisignal.abq0860 (Source: Science Signaling)
Source: Science Signaling - March 22, 2022 Category: Biomedical Science Authors: Annalisa M VanHook Source Type: research