Fecal Microbiota Transplantation Repairs Radiation Enteritis Through Modulating the Gut Microbiota-Mediated Tryptophan Metabolism
In this study, we investigated the efficacy of FMT in alleviating radiation enteritis and explored the mechanisms by multi-omics approaches. Briefly, C57BL/6J mice were subjected to 9 Gy irradiation to the localized abdominal field, and randomized received FMT from healthy donor mice or saline. H&E staining of harvested small intestine showed FMT decreased epithelial injury. Radiation-induced microbiota dysbiosis, characterized by a decrease in beneficial bacteria Lactobacillaceae and Lachnospiraceae, while these bacteria were restored by FMT. Fecal metabolomics analysis revealed that FMT modulated metabolic dysregulat...
Source: Radiation Research - March 31, 2024 Category: Physics Authors: Yeqiang Tu Lumeng Luo Qiong Zhou Juan Ni Qiu Tang Source Type: research
RABiT-III: an Automated Micronucleus Assay at a Non-Specialized Biodosimetry Facility
In this study, we present the application of a similar automated biotech setup at an external high-throughput facility (RABiT-III) to implement the same automated cytokinesis-block micronucleus assay. Specifically, we employed the Agilent BRAVO liquid-handling system and GE IN Cell Analyzer 6000 imaging system in conjunction with the PerkinElmer Columbus image data storage and analysis system. Notably, this analysis system features an embedded PhenoLOGIC machine learning module, simplifying the creation of cell classification algorithms for CBMN assay image analysis and enabling the generation of radiation dose-response cu...
Source: Radiation Research - March 22, 2024 Category: Physics Authors: Mikhail Repin Guy Garty Ralph J Garippa David J Brenner Source Type: research
RABiT-III: an Automated Micronucleus Assay at a Non-Specialized Biodosimetry Facility
In this study, we present the application of a similar automated biotech setup at an external high-throughput facility (RABiT-III) to implement the same automated cytokinesis-block micronucleus assay. Specifically, we employed the Agilent BRAVO liquid-handling system and GE IN Cell Analyzer 6000 imaging system in conjunction with the PerkinElmer Columbus image data storage and analysis system. Notably, this analysis system features an embedded PhenoLOGIC machine learning module, simplifying the creation of cell classification algorithms for CBMN assay image analysis and enabling the generation of radiation dose-response cu...
Source: Radiation Research - March 22, 2024 Category: Physics Authors: Mikhail Repin Guy Garty Ralph J Garippa David J Brenner Source Type: research
RABiT-III: an Automated Micronucleus Assay at a Non-Specialized Biodosimetry Facility
In this study, we present the application of a similar automated biotech setup at an external high-throughput facility (RABiT-III) to implement the same automated cytokinesis-block micronucleus assay. Specifically, we employed the Agilent BRAVO liquid-handling system and GE IN Cell Analyzer 6000 imaging system in conjunction with the PerkinElmer Columbus image data storage and analysis system. Notably, this analysis system features an embedded PhenoLOGIC machine learning module, simplifying the creation of cell classification algorithms for CBMN assay image analysis and enabling the generation of radiation dose-response cu...
Source: Radiation Research - March 22, 2024 Category: Physics Authors: Mikhail Repin Guy Garty Ralph J Garippa David J Brenner Source Type: research
RABiT-III: an Automated Micronucleus Assay at a Non-Specialized Biodosimetry Facility
In this study, we present the application of a similar automated biotech setup at an external high-throughput facility (RABiT-III) to implement the same automated cytokinesis-block micronucleus assay. Specifically, we employed the Agilent BRAVO liquid-handling system and GE IN Cell Analyzer 6000 imaging system in conjunction with the PerkinElmer Columbus image data storage and analysis system. Notably, this analysis system features an embedded PhenoLOGIC machine learning module, simplifying the creation of cell classification algorithms for CBMN assay image analysis and enabling the generation of radiation dose-response cu...
Source: Radiation Research - March 22, 2024 Category: Physics Authors: Mikhail Repin Guy Garty Ralph J Garippa David J Brenner Source Type: research
RABiT-III: an Automated Micronucleus Assay at a Non-Specialized Biodosimetry Facility
In this study, we present the application of a similar automated biotech setup at an external high-throughput facility (RABiT-III) to implement the same automated cytokinesis-block micronucleus assay. Specifically, we employed the Agilent BRAVO liquid-handling system and GE IN Cell Analyzer 6000 imaging system in conjunction with the PerkinElmer Columbus image data storage and analysis system. Notably, this analysis system features an embedded PhenoLOGIC machine learning module, simplifying the creation of cell classification algorithms for CBMN assay image analysis and enabling the generation of radiation dose-response cu...
Source: Radiation Research - March 22, 2024 Category: Physics Authors: Mikhail Repin Guy Garty Ralph J Garippa David J Brenner Source Type: research
RABiT-III: an Automated Micronucleus Assay at a Non-Specialized Biodosimetry Facility
In this study, we present the application of a similar automated biotech setup at an external high-throughput facility (RABiT-III) to implement the same automated cytokinesis-block micronucleus assay. Specifically, we employed the Agilent BRAVO liquid-handling system and GE IN Cell Analyzer 6000 imaging system in conjunction with the PerkinElmer Columbus image data storage and analysis system. Notably, this analysis system features an embedded PhenoLOGIC machine learning module, simplifying the creation of cell classification algorithms for CBMN assay image analysis and enabling the generation of radiation dose-response cu...
Source: Radiation Research - March 22, 2024 Category: Physics Authors: Mikhail Repin Guy Garty Ralph J Garippa David J Brenner Source Type: research
RABiT-III: an Automated Micronucleus Assay at a Non-Specialized Biodosimetry Facility
In this study, we present the application of a similar automated biotech setup at an external high-throughput facility (RABiT-III) to implement the same automated cytokinesis-block micronucleus assay. Specifically, we employed the Agilent BRAVO liquid-handling system and GE IN Cell Analyzer 6000 imaging system in conjunction with the PerkinElmer Columbus image data storage and analysis system. Notably, this analysis system features an embedded PhenoLOGIC machine learning module, simplifying the creation of cell classification algorithms for CBMN assay image analysis and enabling the generation of radiation dose-response cu...
Source: Radiation Research - March 22, 2024 Category: Physics Authors: Mikhail Repin Guy Garty Ralph J Garippa David J Brenner Source Type: research
RABiT-III: an Automated Micronucleus Assay at a Non-Specialized Biodosimetry Facility
In this study, we present the application of a similar automated biotech setup at an external high-throughput facility (RABiT-III) to implement the same automated cytokinesis-block micronucleus assay. Specifically, we employed the Agilent BRAVO liquid-handling system and GE IN Cell Analyzer 6000 imaging system in conjunction with the PerkinElmer Columbus image data storage and analysis system. Notably, this analysis system features an embedded PhenoLOGIC machine learning module, simplifying the creation of cell classification algorithms for CBMN assay image analysis and enabling the generation of radiation dose-response cu...
Source: Radiation Research - March 22, 2024 Category: Physics Authors: Mikhail Repin Guy Garty Ralph J Garippa David J Brenner Source Type: research
Development of a Point-of-Care Microfluidic RNA Extraction Slide for Gene Expression Diagnosis after Irradiation
Radiat Res. 2024 Mar 22. doi: 10.1667/RADE-23-00169.1. Online ahead of print.ABSTRACTIn times of war, radiological/nuclear emergency scenarios have become a reemphasized threat. However, there are challenges in transferring whole-blood samples to laboratories for specialized diagnostics using RNA. This project aims to miniaturize the process of unwieldy conventional RNA extraction with its stationed technical equipment using a microfluidic-based slide (MBS) for point-of-care diagnostics. The MBS is thought to be a preliminary step toward the development of a so-called lab-on-a-chip microfluidic device. A MBS would enable e...
Source: Radiation Research - March 21, 2024 Category: Physics Authors: S Stewart S Motzke C G ärtner W B äumler C Stroszczynski M Port M Abend P Ostheim Source Type: research
Applicability of Gene Expression in Saliva as an Alternative to Blood for Biodosimetry and Prediction of Radiation-induced Health Effects
Radiat Res. 2024 Mar 19. doi: 10.1667/RADE-23-00176.1. Online ahead of print.ABSTRACTAs the great majority of gene expression (GE) biodosimetry studies have been performed using blood as the preferred source of tissue, searching for simple and less-invasive sampling methods is important when considering biodosimetry approaches. Knowing that whole saliva contains an ultrafiltrate of blood and white blood cells, it is expected that the findings in blood can also be found in saliva. This human in vivo study aims to examine radiation-induced GE changes in saliva for biodosimetry purposes and to predict radiation-induced diseas...
Source: Radiation Research - March 18, 2024 Category: Physics Authors: P Ostheim A Tich ý C Badie M Davidkova G Kultova M Markova Stastna I Sirak S Stewart D Schwanke M Kasper S A Ghandhi S A Amundson W B äumler C Stroszczynski M Port M Abend Source Type: research
Applicability of Gene Expression in Saliva as an Alternative to Blood for Biodosimetry and Prediction of Radiation-induced Health Effects
Radiat Res. 2024 Mar 19. doi: 10.1667/RADE-23-00176.1. Online ahead of print.ABSTRACTAs the great majority of gene expression (GE) biodosimetry studies have been performed using blood as the preferred source of tissue, searching for simple and less-invasive sampling methods is important when considering biodosimetry approaches. Knowing that whole saliva contains an ultrafiltrate of blood and white blood cells, it is expected that the findings in blood can also be found in saliva. This human in vivo study aims to examine radiation-induced GE changes in saliva for biodosimetry purposes and to predict radiation-induced diseas...
Source: Radiation Research - March 18, 2024 Category: Physics Authors: P Ostheim A Tich ý C Badie M Davidkova G Kultova M Markova Stastna I Sirak S Stewart D Schwanke M Kasper S A Ghandhi S A Amundson W B äumler C Stroszczynski M Port M Abend Source Type: research
Applicability of Gene Expression in Saliva as an Alternative to Blood for Biodosimetry and Prediction of Radiation-induced Health Effects
Radiat Res. 2024 Mar 19. doi: 10.1667/RADE-23-00176.1. Online ahead of print.ABSTRACTAs the great majority of gene expression (GE) biodosimetry studies have been performed using blood as the preferred source of tissue, searching for simple and less-invasive sampling methods is important when considering biodosimetry approaches. Knowing that whole saliva contains an ultrafiltrate of blood and white blood cells, it is expected that the findings in blood can also be found in saliva. This human in vivo study aims to examine radiation-induced GE changes in saliva for biodosimetry purposes and to predict radiation-induced diseas...
Source: Radiation Research - March 18, 2024 Category: Physics Authors: P Ostheim A Tich ý C Badie M Davidkova G Kultova M Markova Stastna I Sirak S Stewart D Schwanke M Kasper S A Ghandhi S A Amundson W B äumler C Stroszczynski M Port M Abend Source Type: research
Validating a Four-gene Set for H-ARS Severity Prediction in Peripheral Blood Samples of Irradiated Rhesus Macaques
In conclusion, the diagnostic significance for radiation-induced H-ARS severity prediction of FDXR, DDB2, and POU2AF1 was confirmed in this Rhesus macaques model. However, DDB2 showed higher GE values than FDXR. As shown in previous studies, the diagnostic significance of WNT3 could not be reproduced in Rhesus macaques; this could be due to the choice of animal model and methodological challenges.PMID:38471521 | DOI:10.1667/RADE-23-00162.1 (Source: Radiation Research)
Source: Radiation Research - March 12, 2024 Category: Physics Authors: D Schwanke S Sch üle S Stewart O O Fatanmi S Y Wise C Hackenbroch T Wiegel V K Singh M Port M Abend P Ostheim Source Type: research
Emission of Parasitic X Rays of Vacuum-electron Tubes with Glass Housings: Implications for the Evaluation of Occupational Doses
Radiat Res. 2024 Mar 13. doi: 10.1667/RADE-23-00161.1. Online ahead of print.ABSTRACTDespite the large variety of high-voltage semiconductor components for medium and high voltage switching and pulse-forming applications as well as for high-power high-frequency generation, the use of vacuum electron tubes still prevails to a considerable degree. Due to the common design incorporating a high energy electron beam which finally is dumped into an anode or a resonator cavity, these tubes are also considered as sources of X rays produced as bremsstrahlung and characteristic radiation, which are referred to as parasitic X rays. H...
Source: Radiation Research - March 12, 2024 Category: Physics Authors: H P öttgen A Schirmer M Port R Nusshardt Source Type: research
