Product Development within the National Institutes of Health Radiation and Nuclear Countermeasures Program
Radiat Res. 2024 Feb 26. doi: 10.1667/RADE-23-00144.1. Online ahead of print.ABSTRACTThe Radiation and Nuclear Countermeasures Program (RNCP) at the National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH) was established to facilitate the development of medical countermeasures (MCMs) and diagnostic approaches for use in a radiation public health emergency. Approvals for MCMs can be very challenging but are made possible under the United States Food and Drug Administration (FDA) Animal Rule, which is designed to enable licensure of drugs or biologics when clinical efficacy studies ...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: David R Cassatt Andrea L DiCarlo Olivia Molinar-Inglis Source Type: research
Induction of Chromosomal Aberrations after Exposure to the Auger Electron Emitter Iodine-125, the β--emitter Tritium and Cesium-137 γ rays
In this study, we investigated whether DNA lesions induced at different cell cycle stages and by different radiation types [Auger-electrons (125I), β- particles (3H), or γ radiation (137Cs)] have an impact on the number of CA induced after induction of the same number of DSB as determined by the γ-H2AX foci assay. Cells were synchronized and pulse-labeled in S phase with low activities of 125I-UdR or tritiated thymidine. For decay accumulation, cells were cryopreserved either after pulse-labeling in S phase or after progression to G2/M or G1 phase. Experiments with γ irradiation (137Cs) were performed with synchronized...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: M Unverricht-Yeboah M Von Ameln R Kriehuber Source Type: research
Dose Rate Effects on Hydrated Electrons, Hydrogen Peroxide, and a OH Radical Molecular Probe Under Clinical Energy Protons
We report the dose rate dependence of radiation chemical yields (G value) of water radiolysis products under clinical energy protons (230 MeV) to understand mechanisms of the FLASH radiotherapy performed at ultra-high dose rate (>40 Gy/s). The G value of 7-hydoroxy-coumarin-3-carboxylic acid (7OH-C3CA) produced by reactions of coumarin-3-carboxylic acid (C3CA) with OH radicals and oxygen is evaluated by fluorescence method. Also, those of hydrated electrons and hydrogen peroxide are derived by absorption method using Saltzman and Ghomley techniques, respectively. Both G values of 7OH-C3CA and hydrated electrons decrease...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: Tamon Kusumoto Antoine Danvin Taisei Mamiya Aurelia Arnone Severine Chefson Catherine Galindo Philippe Peaupardin Quentin Raffy Nagaaki Kamiguchi Daizo Amano Kenzo Sasai Teruaki Konishi Satoshi Kodaira Source Type: research
Connexin 43 Prevents Radiation-Induced Intestinal Damage via the Ca2+-Dependent PI3K/Akt Signaling Pathway
This study aimed to investigate the expression and role of connexins in RIID and underlying mechanism. In this study, a calcein-AM fluorescence probe was used to detect changes in gap junctional intercellular communication in intestinal epithelial IEC-6 cells. Our results show that gap junctional intercellular communication of IEC-6 cells was reduced at 6, 12, 24, and 48 h after irradiation, with the most pronounced effect at 24 h. Western blotting and immunofluorescence results showed that the expression of Cx43, but not other connexins, was reduced in irradiated intestinal epithelial cells. Silencing of Cx43 reduced gap ...
Source: Radiation Research - February 20, 2024 Category: Physics Authors: Yue Zhu Jun Dai Bin Song Yuehua Zhang Tingyi Yang Hongwei Xu Xiaopeng Xu Yi Gao Tao Yan Weidong Shen Wenhao Zhang Shuyu Zhang Pengfei Liu Source Type: research
Development of a Radiation-induced Pulmonary Fibrosis Partial Body Irradiation Model in C57BL/6 Mice
In conclusion, a long-term model for radiation-induced pulmonary fibrosis was established, and countermeasures could be screened in this model for survival and protection/mitigation or recovery from radiation-induced pulmonary damage.PMID:38376474 | DOI:10.1667/RADE-23-00143.1 (Source: Radiation Research)
Source: Radiation Research - February 20, 2024 Category: Physics Authors: Vidya P Kumar Shalini Jaiswal Kefale Wuddie Jerrold M Ward Mark Lawrence Sanchita P Ghosh Source Type: research
Connexin 43 Prevents Radiation-Induced Intestinal Damage via the Ca2+-Dependent PI3K/Akt Signaling Pathway
This study aimed to investigate the expression and role of connexins in RIID and underlying mechanism. In this study, a calcein-AM fluorescence probe was used to detect changes in gap junctional intercellular communication in intestinal epithelial IEC-6 cells. Our results show that gap junctional intercellular communication of IEC-6 cells was reduced at 6, 12, 24, and 48 h after irradiation, with the most pronounced effect at 24 h. Western blotting and immunofluorescence results showed that the expression of Cx43, but not other connexins, was reduced in irradiated intestinal epithelial cells. Silencing of Cx43 reduced gap ...
Source: Radiation Research - February 20, 2024 Category: Physics Authors: Yue Zhu Jun Dai Bin Song Yuehua Zhang Tingyi Yang Hongwei Xu Xiaopeng Xu Yi Gao Tao Yan Weidong Shen Wenhao Zhang Shuyu Zhang Pengfei Liu Source Type: research
Development of a Radiation-induced Pulmonary Fibrosis Partial Body Irradiation Model in C57BL/6 Mice
In conclusion, a long-term model for radiation-induced pulmonary fibrosis was established, and countermeasures could be screened in this model for survival and protection/mitigation or recovery from radiation-induced pulmonary damage.PMID:38376474 | DOI:10.1667/RADE-23-00143.1 (Source: Radiation Research)
Source: Radiation Research - February 20, 2024 Category: Physics Authors: Vidya P Kumar Shalini Jaiswal Kefale Wuddie Jerrold M Ward Mark Lawrence Sanchita P Ghosh Source Type: research
Connexin 43 Prevents Radiation-Induced Intestinal Damage via the Ca2+-Dependent PI3K/Akt Signaling Pathway
This study aimed to investigate the expression and role of connexins in RIID and underlying mechanism. In this study, a calcein-AM fluorescence probe was used to detect changes in gap junctional intercellular communication in intestinal epithelial IEC-6 cells. Our results show that gap junctional intercellular communication of IEC-6 cells was reduced at 6, 12, 24, and 48 h after irradiation, with the most pronounced effect at 24 h. Western blotting and immunofluorescence results showed that the expression of Cx43, but not other connexins, was reduced in irradiated intestinal epithelial cells. Silencing of Cx43 reduced gap ...
Source: Radiation Research - February 20, 2024 Category: Physics Authors: Yue Zhu Jun Dai Bin Song Yuehua Zhang Tingyi Yang Hongwei Xu Xiaopeng Xu Yi Gao Tao Yan Weidong Shen Wenhao Zhang Shuyu Zhang Pengfei Liu Source Type: research
Development of a Radiation-induced Pulmonary Fibrosis Partial Body Irradiation Model in C57BL/6 Mice
In conclusion, a long-term model for radiation-induced pulmonary fibrosis was established, and countermeasures could be screened in this model for survival and protection/mitigation or recovery from radiation-induced pulmonary damage.PMID:38376474 | DOI:10.1667/RADE-23-00143.1 (Source: Radiation Research)
Source: Radiation Research - February 20, 2024 Category: Physics Authors: Vidya P Kumar Shalini Jaiswal Kefale Wuddie Jerrold M Ward Mark Lawrence Sanchita P Ghosh Source Type: research
Connexin 43 Prevents Radiation-Induced Intestinal Damage via the Ca2+-Dependent PI3K/Akt Signaling Pathway
This study aimed to investigate the expression and role of connexins in RIID and underlying mechanism. In this study, a calcein-AM fluorescence probe was used to detect changes in gap junctional intercellular communication in intestinal epithelial IEC-6 cells. Our results show that gap junctional intercellular communication of IEC-6 cells was reduced at 6, 12, 24, and 48 h after irradiation, with the most pronounced effect at 24 h. Western blotting and immunofluorescence results showed that the expression of Cx43, but not other connexins, was reduced in irradiated intestinal epithelial cells. Silencing of Cx43 reduced gap ...
Source: Radiation Research - February 20, 2024 Category: Physics Authors: Yue Zhu Jun Dai Bin Song Yuehua Zhang Tingyi Yang Hongwei Xu Xiaopeng Xu Yi Gao Tao Yan Weidong Shen Wenhao Zhang Shuyu Zhang Pengfei Liu Source Type: research
Development of a Radiation-induced Pulmonary Fibrosis Partial Body Irradiation Model in C57BL/6 Mice
In conclusion, a long-term model for radiation-induced pulmonary fibrosis was established, and countermeasures could be screened in this model for survival and protection/mitigation or recovery from radiation-induced pulmonary damage.PMID:38376474 | DOI:10.1667/RADE-23-00143.1 (Source: Radiation Research)
Source: Radiation Research - February 20, 2024 Category: Physics Authors: Vidya P Kumar Shalini Jaiswal Kefale Wuddie Jerrold M Ward Mark Lawrence Sanchita P Ghosh Source Type: research
Connexin 43 Prevents Radiation-Induced Intestinal Damage via the Ca2+-Dependent PI3K/Akt Signaling Pathway
This study aimed to investigate the expression and role of connexins in RIID and underlying mechanism. In this study, a calcein-AM fluorescence probe was used to detect changes in gap junctional intercellular communication in intestinal epithelial IEC-6 cells. Our results show that gap junctional intercellular communication of IEC-6 cells was reduced at 6, 12, 24, and 48 h after irradiation, with the most pronounced effect at 24 h. Western blotting and immunofluorescence results showed that the expression of Cx43, but not other connexins, was reduced in irradiated intestinal epithelial cells. Silencing of Cx43 reduced gap ...
Source: Radiation Research - February 20, 2024 Category: Physics Authors: Yue Zhu Jun Dai Bin Song Yuehua Zhang Tingyi Yang Hongwei Xu Xiaopeng Xu Yi Gao Tao Yan Weidong Shen Wenhao Zhang Shuyu Zhang Pengfei Liu Source Type: research
Development of a Radiation-induced Pulmonary Fibrosis Partial Body Irradiation Model in C57BL/6 Mice
In conclusion, a long-term model for radiation-induced pulmonary fibrosis was established, and countermeasures could be screened in this model for survival and protection/mitigation or recovery from radiation-induced pulmonary damage.PMID:38376474 | DOI:10.1667/RADE-23-00143.1 (Source: Radiation Research)
Source: Radiation Research - February 20, 2024 Category: Physics Authors: Vidya P Kumar Shalini Jaiswal Kefale Wuddie Jerrold M Ward Mark Lawrence Sanchita P Ghosh Source Type: research
Connexin 43 Prevents Radiation-Induced Intestinal Damage via the Ca2+-Dependent PI3K/Akt Signaling Pathway
This study aimed to investigate the expression and role of connexins in RIID and underlying mechanism. In this study, a calcein-AM fluorescence probe was used to detect changes in gap junctional intercellular communication in intestinal epithelial IEC-6 cells. Our results show that gap junctional intercellular communication of IEC-6 cells was reduced at 6, 12, 24, and 48 h after irradiation, with the most pronounced effect at 24 h. Western blotting and immunofluorescence results showed that the expression of Cx43, but not other connexins, was reduced in irradiated intestinal epithelial cells. Silencing of Cx43 reduced gap ...
Source: Radiation Research - February 20, 2024 Category: Physics Authors: Yue Zhu Jun Dai Bin Song Yuehua Zhang Tingyi Yang Hongwei Xu Xiaopeng Xu Yi Gao Tao Yan Weidong Shen Wenhao Zhang Shuyu Zhang Pengfei Liu Source Type: research
Development of a Radiation-induced Pulmonary Fibrosis Partial Body Irradiation Model in C57BL/6 Mice
In conclusion, a long-term model for radiation-induced pulmonary fibrosis was established, and countermeasures could be screened in this model for survival and protection/mitigation or recovery from radiation-induced pulmonary damage.PMID:38376474 | DOI:10.1667/RADE-23-00143.1 (Source: Radiation Research)
Source: Radiation Research - February 20, 2024 Category: Physics Authors: Vidya P Kumar Shalini Jaiswal Kefale Wuddie Jerrold M Ward Mark Lawrence Sanchita P Ghosh Source Type: research
