Product Development within the National Institutes of Health Radiation and Nuclear Countermeasures Program
Radiat Res. 2024 Feb 26. doi: 10.1667/RADE-23-00144.1. Online ahead of print.ABSTRACTThe Radiation and Nuclear Countermeasures Program (RNCP) at the National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH) was established to facilitate the development of medical countermeasures (MCMs) and diagnostic approaches for use in a radiation public health emergency. Approvals for MCMs can be very challenging but are made possible under the United States Food and Drug Administration (FDA) Animal Rule, which is designed to enable licensure of drugs or biologics when clinical efficacy studies ...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: David R Cassatt Andrea L DiCarlo Olivia Molinar-Inglis Source Type: research
Induction of Chromosomal Aberrations after Exposure to the Auger Electron Emitter Iodine-125, the β--emitter Tritium and Cesium-137 γ rays
In this study, we investigated whether DNA lesions induced at different cell cycle stages and by different radiation types [Auger-electrons (125I), β- particles (3H), or γ radiation (137Cs)] have an impact on the number of CA induced after induction of the same number of DSB as determined by the γ-H2AX foci assay. Cells were synchronized and pulse-labeled in S phase with low activities of 125I-UdR or tritiated thymidine. For decay accumulation, cells were cryopreserved either after pulse-labeling in S phase or after progression to G2/M or G1 phase. Experiments with γ irradiation (137Cs) were performed with synchronized...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: M Unverricht-Yeboah M Von Ameln R Kriehuber Source Type: research
Dose Rate Effects on Hydrated Electrons, Hydrogen Peroxide, and a OH Radical Molecular Probe Under Clinical Energy Protons
We report the dose rate dependence of radiation chemical yields (G value) of water radiolysis products under clinical energy protons (230 MeV) to understand mechanisms of the FLASH radiotherapy performed at ultra-high dose rate (>40 Gy/s). The G value of 7-hydoroxy-coumarin-3-carboxylic acid (7OH-C3CA) produced by reactions of coumarin-3-carboxylic acid (C3CA) with OH radicals and oxygen is evaluated by fluorescence method. Also, those of hydrated electrons and hydrogen peroxide are derived by absorption method using Saltzman and Ghomley techniques, respectively. Both G values of 7OH-C3CA and hydrated electrons decrease...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: Tamon Kusumoto Antoine Danvin Taisei Mamiya Aurelia Arnone Severine Chefson Catherine Galindo Philippe Peaupardin Quentin Raffy Nagaaki Kamiguchi Daizo Amano Kenzo Sasai Teruaki Konishi Satoshi Kodaira Source Type: research
Product Development within the National Institutes of Health Radiation and Nuclear Countermeasures Program
Radiat Res. 2024 Feb 26. doi: 10.1667/RADE-23-00144.1. Online ahead of print.ABSTRACTThe Radiation and Nuclear Countermeasures Program (RNCP) at the National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH) was established to facilitate the development of medical countermeasures (MCMs) and diagnostic approaches for use in a radiation public health emergency. Approvals for MCMs can be very challenging but are made possible under the United States Food and Drug Administration (FDA) Animal Rule, which is designed to enable licensure of drugs or biologics when clinical efficacy studies ...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: David R Cassatt Andrea L DiCarlo Olivia Molinar-Inglis Source Type: research
Induction of Chromosomal Aberrations after Exposure to the Auger Electron Emitter Iodine-125, the β--emitter Tritium and Cesium-137 γ rays
In this study, we investigated whether DNA lesions induced at different cell cycle stages and by different radiation types [Auger-electrons (125I), β- particles (3H), or γ radiation (137Cs)] have an impact on the number of CA induced after induction of the same number of DSB as determined by the γ-H2AX foci assay. Cells were synchronized and pulse-labeled in S phase with low activities of 125I-UdR or tritiated thymidine. For decay accumulation, cells were cryopreserved either after pulse-labeling in S phase or after progression to G2/M or G1 phase. Experiments with γ irradiation (137Cs) were performed with synchronized...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: M Unverricht-Yeboah M Von Ameln R Kriehuber Source Type: research
Dose Rate Effects on Hydrated Electrons, Hydrogen Peroxide, and a OH Radical Molecular Probe Under Clinical Energy Protons
We report the dose rate dependence of radiation chemical yields (G value) of water radiolysis products under clinical energy protons (230 MeV) to understand mechanisms of the FLASH radiotherapy performed at ultra-high dose rate (>40 Gy/s). The G value of 7-hydoroxy-coumarin-3-carboxylic acid (7OH-C3CA) produced by reactions of coumarin-3-carboxylic acid (C3CA) with OH radicals and oxygen is evaluated by fluorescence method. Also, those of hydrated electrons and hydrogen peroxide are derived by absorption method using Saltzman and Ghomley techniques, respectively. Both G values of 7OH-C3CA and hydrated electrons decrease...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: Tamon Kusumoto Antoine Danvin Taisei Mamiya Aurelia Arnone Severine Chefson Catherine Galindo Philippe Peaupardin Quentin Raffy Nagaaki Kamiguchi Daizo Amano Kenzo Sasai Teruaki Konishi Satoshi Kodaira Source Type: research
Product Development within the National Institutes of Health Radiation and Nuclear Countermeasures Program
Radiat Res. 2024 Feb 26. doi: 10.1667/RADE-23-00144.1. Online ahead of print.ABSTRACTThe Radiation and Nuclear Countermeasures Program (RNCP) at the National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH) was established to facilitate the development of medical countermeasures (MCMs) and diagnostic approaches for use in a radiation public health emergency. Approvals for MCMs can be very challenging but are made possible under the United States Food and Drug Administration (FDA) Animal Rule, which is designed to enable licensure of drugs or biologics when clinical efficacy studies ...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: David R Cassatt Andrea L DiCarlo Olivia Molinar-Inglis Source Type: research
Induction of Chromosomal Aberrations after Exposure to the Auger Electron Emitter Iodine-125, the β--emitter Tritium and Cesium-137 γ rays
In this study, we investigated whether DNA lesions induced at different cell cycle stages and by different radiation types [Auger-electrons (125I), β- particles (3H), or γ radiation (137Cs)] have an impact on the number of CA induced after induction of the same number of DSB as determined by the γ-H2AX foci assay. Cells were synchronized and pulse-labeled in S phase with low activities of 125I-UdR or tritiated thymidine. For decay accumulation, cells were cryopreserved either after pulse-labeling in S phase or after progression to G2/M or G1 phase. Experiments with γ irradiation (137Cs) were performed with synchronized...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: M Unverricht-Yeboah M Von Ameln R Kriehuber Source Type: research
Dose Rate Effects on Hydrated Electrons, Hydrogen Peroxide, and a OH Radical Molecular Probe Under Clinical Energy Protons
We report the dose rate dependence of radiation chemical yields (G value) of water radiolysis products under clinical energy protons (230 MeV) to understand mechanisms of the FLASH radiotherapy performed at ultra-high dose rate (>40 Gy/s). The G value of 7-hydoroxy-coumarin-3-carboxylic acid (7OH-C3CA) produced by reactions of coumarin-3-carboxylic acid (C3CA) with OH radicals and oxygen is evaluated by fluorescence method. Also, those of hydrated electrons and hydrogen peroxide are derived by absorption method using Saltzman and Ghomley techniques, respectively. Both G values of 7OH-C3CA and hydrated electrons decrease...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: Tamon Kusumoto Antoine Danvin Taisei Mamiya Aurelia Arnone Severine Chefson Catherine Galindo Philippe Peaupardin Quentin Raffy Nagaaki Kamiguchi Daizo Amano Kenzo Sasai Teruaki Konishi Satoshi Kodaira Source Type: research
Product Development within the National Institutes of Health Radiation and Nuclear Countermeasures Program
Radiat Res. 2024 Feb 26. doi: 10.1667/RADE-23-00144.1. Online ahead of print.ABSTRACTThe Radiation and Nuclear Countermeasures Program (RNCP) at the National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH) was established to facilitate the development of medical countermeasures (MCMs) and diagnostic approaches for use in a radiation public health emergency. Approvals for MCMs can be very challenging but are made possible under the United States Food and Drug Administration (FDA) Animal Rule, which is designed to enable licensure of drugs or biologics when clinical efficacy studies ...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: David R Cassatt Andrea L DiCarlo Olivia Molinar-Inglis Source Type: research
Induction of Chromosomal Aberrations after Exposure to the Auger Electron Emitter Iodine-125, the β--emitter Tritium and Cesium-137 γ rays
In this study, we investigated whether DNA lesions induced at different cell cycle stages and by different radiation types [Auger-electrons (125I), β- particles (3H), or γ radiation (137Cs)] have an impact on the number of CA induced after induction of the same number of DSB as determined by the γ-H2AX foci assay. Cells were synchronized and pulse-labeled in S phase with low activities of 125I-UdR or tritiated thymidine. For decay accumulation, cells were cryopreserved either after pulse-labeling in S phase or after progression to G2/M or G1 phase. Experiments with γ irradiation (137Cs) were performed with synchronized...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: M Unverricht-Yeboah M Von Ameln R Kriehuber Source Type: research
Dose Rate Effects on Hydrated Electrons, Hydrogen Peroxide, and a OH Radical Molecular Probe Under Clinical Energy Protons
We report the dose rate dependence of radiation chemical yields (G value) of water radiolysis products under clinical energy protons (230 MeV) to understand mechanisms of the FLASH radiotherapy performed at ultra-high dose rate (>40 Gy/s). The G value of 7-hydoroxy-coumarin-3-carboxylic acid (7OH-C3CA) produced by reactions of coumarin-3-carboxylic acid (C3CA) with OH radicals and oxygen is evaluated by fluorescence method. Also, those of hydrated electrons and hydrogen peroxide are derived by absorption method using Saltzman and Ghomley techniques, respectively. Both G values of 7OH-C3CA and hydrated electrons decrease...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: Tamon Kusumoto Antoine Danvin Taisei Mamiya Aurelia Arnone Severine Chefson Catherine Galindo Philippe Peaupardin Quentin Raffy Nagaaki Kamiguchi Daizo Amano Kenzo Sasai Teruaki Konishi Satoshi Kodaira Source Type: research
Product Development within the National Institutes of Health Radiation and Nuclear Countermeasures Program
Radiat Res. 2024 Feb 26. doi: 10.1667/RADE-23-00144.1. Online ahead of print.ABSTRACTThe Radiation and Nuclear Countermeasures Program (RNCP) at the National Institute of Allergy and Infectious Diseases (NIAID), National Institutes of Health (NIH) was established to facilitate the development of medical countermeasures (MCMs) and diagnostic approaches for use in a radiation public health emergency. Approvals for MCMs can be very challenging but are made possible under the United States Food and Drug Administration (FDA) Animal Rule, which is designed to enable licensure of drugs or biologics when clinical efficacy studies ...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: David R Cassatt Andrea L DiCarlo Olivia Molinar-Inglis Source Type: research
Induction of Chromosomal Aberrations after Exposure to the Auger Electron Emitter Iodine-125, the β--emitter Tritium and Cesium-137 γ rays
In this study, we investigated whether DNA lesions induced at different cell cycle stages and by different radiation types [Auger-electrons (125I), β- particles (3H), or γ radiation (137Cs)] have an impact on the number of CA induced after induction of the same number of DSB as determined by the γ-H2AX foci assay. Cells were synchronized and pulse-labeled in S phase with low activities of 125I-UdR or tritiated thymidine. For decay accumulation, cells were cryopreserved either after pulse-labeling in S phase or after progression to G2/M or G1 phase. Experiments with γ irradiation (137Cs) were performed with synchronized...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: M Unverricht-Yeboah M Von Ameln R Kriehuber Source Type: research
Dose Rate Effects on Hydrated Electrons, Hydrogen Peroxide, and a OH Radical Molecular Probe Under Clinical Energy Protons
We report the dose rate dependence of radiation chemical yields (G value) of water radiolysis products under clinical energy protons (230 MeV) to understand mechanisms of the FLASH radiotherapy performed at ultra-high dose rate (>40 Gy/s). The G value of 7-hydoroxy-coumarin-3-carboxylic acid (7OH-C3CA) produced by reactions of coumarin-3-carboxylic acid (C3CA) with OH radicals and oxygen is evaluated by fluorescence method. Also, those of hydrated electrons and hydrogen peroxide are derived by absorption method using Saltzman and Ghomley techniques, respectively. Both G values of 7OH-C3CA and hydrated electrons decrease...
Source: Radiation Research - February 26, 2024 Category: Physics Authors: Tamon Kusumoto Antoine Danvin Taisei Mamiya Aurelia Arnone Severine Chefson Catherine Galindo Philippe Peaupardin Quentin Raffy Nagaaki Kamiguchi Daizo Amano Kenzo Sasai Teruaki Konishi Satoshi Kodaira Source Type: research
