Upregulation of XRN2 acts as an oncogene in oral squamous cell carcinoma and correlates with poor prognosis
CONCLUSION: XRN2 is significantly overexpressed in OSCC tissues and its upregulation was closely associated with poor prognosis of OSCC patients. XRN2 could be a useful prognostic biomarker and a potential therapeutic target for OSCC.PMID:33626405 | DOI:10.1016/j.prp.2021.153355 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 24, 2021 Category: Pathology Authors: Jia-Cheng Liu Ling Gao Shao-Ming Li Jing-Jing Zheng Da-Gang Li Ke-Qian Zhi Wen-Hao Ren Source Type: research

MicroRNA signature in liver cancer
Pathol Res Pract. 2021 Feb 9;219:153369. doi: 10.1016/j.prp.2021.153369. Online ahead of print.ABSTRACTLiver cancer is the 7th utmost frequent neoplasm and the 4th principal source of cancer deaths. This malignancy is linked with several environmental and lifestyle-related factors emphasizing the role of epigenetics in its pathogenesis. MicroRNAs (miRNAs) have been regarded as potent epigenetic mechanisms partaking in the pathogenesis of liver cancer. Dysregulation of miRNAs has been related with poor outcome of patients with liver cancer. In the current manuscript, we provide a concise review of the results of recent stud...
Source: Pathology, Research and Practice - February 24, 2021 Category: Pathology Authors: Soudeh Ghafouri-Fard Kasra Honarmand Tamizkar Bashdar Mahmud Hussen Mohammad Taheri Source Type: research

Prominent roles of ribosomal S6 kinase 4 (RSK4) in cancer
Pathol Res Pract. 2021 Feb 12;219:153374. doi: 10.1016/j.prp.2021.153374. Online ahead of print.ABSTRACTRSK4 refers to one Ser/Thr protein kinase functioning downstream pertaining to the signaling channel of protein kinase (MAPK) stimulated by Ras/mitogen. RSK4 can regulate numerous substrates impacting cells' surviving state, growing processes and proliferating process. Thus, dysregulated RSK4 active state display a relationship to several carcinoma categories, covering breast carcinoma, esophageal squamous cell carcinoma, glioma, colorectal carcinoma, lung carcinoma, ovarian carcinoma, leukemia, endometrial carcinoma, an...
Source: Pathology, Research and Practice - February 23, 2021 Category: Pathology Authors: Junpeng Xu Qingge Jia Yan Zhang Yuan Yuan Tianqi Xu Kangjie Yu Jia Chai Kaijing Wang Ligang Chen Tian Xiao Mingyang Li Source Type: research

Mutation and expression alterations of histone methylation-related NSD2, KDM2B and SETMAR genes in colon cancers
In this study, we analyzed mononucleotide repeats in coding sequences of NSD2, KDM2B and SETMAR genes, and found frameshift mutations in 10 %, 2 % and 1 % of CCs with MSI-H, respectively. Of note, there was no frameshift mutation of these genes in microsatellite stable (MSS) CCs. In addition, we discovered that 2 and 2 of 16 CRCs (12.5 % and 12.5 %) harbored intratumoral heterogeneity (ITH) of the NSD2 and KDM2B frameshift mutations, respectively. In the immunohistochemistry for NSD2, intensity of NSD2 immunostaining in MSI-H CC is decreased compared to that in MSS. These results suggest that NSD2 might be altered at multi...
Source: Pathology, Research and Practice - February 23, 2021 Category: Pathology Authors: Seong Won Moon Hyun Ji Son Ha Yoon Mo Eun Ji Choi Nam Jin Yoo Sug Hyung Lee Source Type: research

The role of altered long noncoding RNAs in overall survival of ovarian cancer: A systematic review and meta-analysis
Pathol Res Pract. 2021 Feb 9;219:153363. doi: 10.1016/j.prp.2021.153363. Online ahead of print.ABSTRACTIn recent years, tremendous research efforts have been focused on investigating the effect of dysregulation of lncRNAs on cancer progression, most of which confirm a positive link. This inspired us to conduct the present meta-analysis to explore whether aberrant expression of multiple lncRNAs has a role in patients' outcome in ovarian cancer. This comprehensive meta-analysis pertains to the evaluation of association between dysregulated lncRNAs expression level with eventual outcome and clinicopathological characteristics...
Source: Pathology, Research and Practice - February 23, 2021 Category: Pathology Authors: Elahe Seyed Hosseini Marziyeh Alizadeh Zarei Hamed Haddad Kashani Alireza Milajerdi Zahra Zare Dehghanani Hassan Hassani Bafrani Hossein Nikzad Source Type: research

Prominent roles of ribosomal S6 kinase 4 (RSK4) in cancer
Pathol Res Pract. 2021 Feb 12;219:153374. doi: 10.1016/j.prp.2021.153374. Online ahead of print.ABSTRACTRSK4 refers to one Ser/Thr protein kinase functioning downstream pertaining to the signaling channel of protein kinase (MAPK) stimulated by Ras/mitogen. RSK4 can regulate numerous substrates impacting cells' surviving state, growing processes and proliferating process. Thus, dysregulated RSK4 active state display a relationship to several carcinoma categories, covering breast carcinoma, esophageal squamous cell carcinoma, glioma, colorectal carcinoma, lung carcinoma, ovarian carcinoma, leukemia, endometrial carcinoma, an...
Source: Pathology, Research and Practice - February 23, 2021 Category: Pathology Authors: Junpeng Xu Qingge Jia Yan Zhang Yuan Yuan Tianqi Xu Kangjie Yu Jia Chai Kaijing Wang Ligang Chen Tian Xiao Mingyang Li Source Type: research

Mutation and expression alterations of histone methylation-related NSD2, KDM2B and SETMAR genes in colon cancers
In this study, we analyzed mononucleotide repeats in coding sequences of NSD2, KDM2B and SETMAR genes, and found frameshift mutations in 10 %, 2 % and 1 % of CCs with MSI-H, respectively. Of note, there was no frameshift mutation of these genes in microsatellite stable (MSS) CCs. In addition, we discovered that 2 and 2 of 16 CRCs (12.5 % and 12.5 %) harbored intratumoral heterogeneity (ITH) of the NSD2 and KDM2B frameshift mutations, respectively. In the immunohistochemistry for NSD2, intensity of NSD2 immunostaining in MSI-H CC is decreased compared to that in MSS. These results suggest that NSD2 might be altered at multi...
Source: Pathology, Research and Practice - February 23, 2021 Category: Pathology Authors: Seong Won Moon Hyun Ji Son Ha Yoon Mo Eun Ji Choi Nam Jin Yoo Sug Hyung Lee Source Type: research

The role of altered long noncoding RNAs in overall survival of ovarian cancer: A systematic review and meta-analysis
Pathol Res Pract. 2021 Feb 9;219:153363. doi: 10.1016/j.prp.2021.153363. Online ahead of print.ABSTRACTIn recent years, tremendous research efforts have been focused on investigating the effect of dysregulation of lncRNAs on cancer progression, most of which confirm a positive link. This inspired us to conduct the present meta-analysis to explore whether aberrant expression of multiple lncRNAs has a role in patients' outcome in ovarian cancer. This comprehensive meta-analysis pertains to the evaluation of association between dysregulated lncRNAs expression level with eventual outcome and clinicopathological characteristics...
Source: Pathology, Research and Practice - February 23, 2021 Category: Pathology Authors: Elahe Seyed Hosseini Marziyeh Alizadeh Zarei Hamed Haddad Kashani Alireza Milajerdi Zahra Zare Dehghanani Hassan Hassani Bafrani Hossein Nikzad Source Type: research

Prognostic value of Bcl2 and p53 in Hodgkin lymphoma: A systematic review and meta-analysis
Pathol Res Pract. 2021 Feb 8;219:153370. doi: 10.1016/j.prp.2021.153370. Online ahead of print.ABSTRACTAIMS: Several studies suggested that high expression of Bcl2 and/or p53 in Hodgkin/Reed-Sternberg cells is an unfavorable prognostic factor in Hodgkin lymphoma (HL). However, results in this field appear contrasting. We aimed to assess the prognostic value of p53 and Bcl2 in HL through a systematic review and meta-analysis.METHODS: Electronic databases were searched from January 2000 to December 2020 for all studies assessing the prognostic value of p53 and Bcl2 in HL. The association of high p53 or Bcl2 expression with o...
Source: Pathology, Research and Practice - February 22, 2021 Category: Pathology Authors: Antonio Travaglino Daniela Russo Silvia Varricchio Marco Picardi Massimo Mascolo Source Type: research

MicroRNA-92b-3p promotes the progression of liver fibrosis by targeting CREB3L2 through the JAK/STAT signaling pathway
Pathol Res Pract. 2021 Feb 8;219:153367. doi: 10.1016/j.prp.2021.153367. Online ahead of print.ABSTRACTLiver fibrosis is a common feature of almost all chronic liver diseases, which eventually leads to cirrhosis and even hepatocellular carcinoma (HCC). The current study showed that miR-92b plays an important role in the progression of HCC but its role in liver fibrosis is still unclear. Here we aimed to explore the role and underlying molecular mechanism of miR-92b-3p in the activated hepatic stellate cells (HSCs) and the pathological process of hepatic fibrosis. We found that miR-92b-3p was highly expressed both in fibrot...
Source: Pathology, Research and Practice - February 22, 2021 Category: Pathology Authors: Weirong Huang Ran Ji Sijia Ge Danhua Zhou Zhaoxiu Liu Ying Sun Wei Huang Cuihua Lu Source Type: research

Erratum to "BRAF and NRAS mutations and antitumor immunity in Korean malignant melanomas and their prognostic relevance: Gene set enrichment analysis and CIBERSORT analysis" [Pathol. Res. Pract. 215 (12) (2019) 152671]
Pathol Res Pract. 2021 Feb 19;219:153316. doi: 10.1016/j.prp.2020.153316. Online ahead of print.NO ABSTRACTPMID:33618249 | DOI:10.1016/j.prp.2020.153316 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 22, 2021 Category: Pathology Authors: Kyueng-Whan Min Ji-Young Choe Mi Jung Kwon Hye Kyung Lee Ho Suk Kang Eun Sook Nam Seong Jin Cho Hye-Rim Park Soo Kee Min Jinwon Seo Yun Joong Kim Nan Young Kim Ho Young Kim Source Type: research

Neuroendocrine differentiation in a large series of genetically-confirmed Ewing's sarcoma family tumor: Does it provide any diagnostic or prognostic information?
Pathol Res Pract. 2021 Feb 6;219:153362. doi: 10.1016/j.prp.2021.153362. Online ahead of print.ABSTRACTGiven the potential for neuroendocrine differentiation in Ewing's sarcoma family of tumors (ESFT), we aimed to determine neuroendocrine expression in a large series of genetically-confirmed ESFT and its prognostic significance in clinically-localised neoplasms (n = 176). Slides prepared from tissue microarrays were stained for Insulinoma-associated protein 1 (INSM1), CD56, chromogranin-A and synaptophysin. INSM1 expression was present in 59% of ESFT, while synaptophysin, chromogranin-A and CD56 were expressed in only 13%,...
Source: Pathology, Research and Practice - February 21, 2021 Category: Pathology Authors: Isidro Machado Samuel Navarro Jos é A López-Guerrero Laura Verdini Piero Picci Francisco Giner Antonio Llombart-Bosch Source Type: research

Squamous differentiation is a potential biomarker predicting tumor progression in patients treated with pembrolizumab for urothelial carcinoma
This study aims to clarify the predictors of disease progression for ICI treatment in patients with urothelial carcinoma. We analyzed the response pattern of 23 urothelial carcinomas treated with pembrolizumab and its association with pathological features and potential immunohistochemical markers including EGFR, MDM2, p53, p16, and programmed cell death ligand-1 (PD-L1) expression and CD8- and CD204-positive cell infiltration. During ICI therapy, 13 (57 %) patients showed progressive disease including 6 (26 %) with hyperprogressive disease. Notably, squamous differentiation combined with MAC387 expression was observed exc...
Source: Pathology, Research and Practice - February 21, 2021 Category: Pathology Authors: Yu Miyama Teppei Morikawa Jimpei Miyakawa Yuichi Koyama Taketo Kawai Haruki Kume Tetsuo Ushiku Source Type: research

Angiogenesis regulation by microRNAs and long non-coding RNAs in human breast cancer
Pathol Res Pract. 2021 Jan 13;219:153326. doi: 10.1016/j.prp.2020.153326. Online ahead of print.ABSTRACTMicroRNAs (miRNAs) and long non-coding RNAs (lncRNAs) are capable of regulating gene expression post-transcriptionally. Since the past decade, a number of in vitro, in vivo, and clinical studies reported the roles of these non-coding RNAs (ncRNAs) in regulating angiogenesis, an important cancer hallmark that is associated with metastases and poor prognosis. The specific roles of various miRNAs and lncRNAs in regulating angiogenesis in breast cancer, with particular focus on the downstream targets and signalling pathways ...
Source: Pathology, Research and Practice - February 18, 2021 Category: Pathology Authors: Zhi Xiong Chong Swee Keong Yeap Wan Yong Ho Source Type: research

Three cases of synovial sarcoma of gastric wall: A case report and review of the literature
We report here three cases of primary gastric synovial sarcoma. Malignant gastric mesenchymal tumor has many differential diagnoses other than synovial sarcoma, such as gastrointestinal stromal tumor (GIST), leiomyosarcoma, schwannoma, malignant peripheral nerve sheath tumor (MPNST) and so on. In our three cases, using reverse transcription polymerase chain reaction (RT-PCR) and direct sequencing, we detected an SS18-SSX1 fusion gene, which is specific to synovial sarcoma. In addition, we found the reduced expression of SMARCB1/INI1 in the tumor cells in two of the three cases. Through histopathological, immunohistochemica...
Source: Pathology, Research and Practice - February 14, 2021 Category: Pathology Authors: Yusuke Kuboyama Yuichi Yamada Kenichi Kohashi Yu Toda Koji Kawakami Daichi Kitahara Kenichi Nishiyama Yuka Hiraki Masafumi Oya Yoshinao Oda Source Type: research

SMARCA4 (BRG1) and SMARCB1 (INI1) expression in TTF-1 negative neuroendocrine carcinomas including merkel cell carcinoma
The objective of this study was to investigate the loss of BRG1 and INI1 expression in TTF-1 negative neuroendocrine carcinomas to see if they are analogous to small-cell carcinoma of the ovary, hypercalcemic type. The potential role of these tumor suppressor genes in high-grade neuroendocrine carcinoma largely remains unknown. Cases of previously diagnosed Small cell carcinoma (SmCC), Large cell neuroendocrine carcinoma (LCNEC) and Merkel cell carcinoma (MCC) were selected. Immunohistochemical expression patterns for BRG1 and INI1 were interpreted as: intact, hybrid and complete loss of nuclear staining. SmCC and LCNEC ca...
Source: Pathology, Research and Practice - February 13, 2021 Category: Pathology Authors: Jatin S Gandhi Fnu Alnoor Qandeel Sadiq Jorge Solares Joel F Gradowski Source Type: research

Fibroblast function in COVID-19
Pathol Res Pract. 2021 Feb 1;219:153353. doi: 10.1016/j.prp.2021.153353. Online ahead of print.NO ABSTRACTPMID:33561763 | PMC:PMC7849528 | DOI:10.1016/j.prp.2021.153353 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 9, 2021 Category: Pathology Authors: Mustafa Sahin Erman Akkus Source Type: research

Broad spectrum mutational analysis of chromophobe renal cell carcinoma using next-generation sequencing
CONCLUSION: ChRCC is a rare disease still not fully molecularly characterized. Next-generation sequencing analysis could be useful to identify potential mutation with prognostic value or that could be potential therapeutic targets.PMID:33556910 | DOI:10.1016/j.prp.2021.153350 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 8, 2021 Category: Pathology Authors: Veronica Mollica Tania Franceschini Elisa Gruppioni Alessandro Rizzo Costantino Ricci Riccardo Schiavina Eugenio Brunocilla Andrea Ardizzoni Michelangelo Fiorentino Francesca Giunchi Francesco Massari Source Type: research

WITHDRAWN: Diagnostic performance of MASP-2 in the diagnosis of colorectal carcinoma
This article has been withdrawn: please see Elsevier Policy on Article Withdrawal (http://www.elsevier.com/locate/withdrawalpolicy). The publisher regrets that an error occurred which led to the premature publication of this paper. The publisher apologizes to the authors and the readers for this unfortunate error.PMID:33549364 | DOI:10.1016/j.prp.2020.153278 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 7, 2021 Category: Pathology Authors: Keqian Zhang Tianqi Mao Zhicheng He Xiaojiao Wu Yu Peng Yanrong Chen Yan Dong Zhihua Ruan Zhe Wang Source Type: research

Wnt (canonical and non canonical) pathways in breast carcinoma with extensive vascular invasion and inflammatory breast carcinoma
CONCLUSIONS: Canonical Wnt pathway is involved in malignant progression with dominant role for vascular invasion whereas non canonical Wnt pathway plays an important role on survival time including the capacity to identify carcinomas with IBC-like prognosis. Furthermore ePVI may represent a "prodromal form of IBC" as demonstrated by its clinicopathological and biological similarity with IBC.PMID:33550148 | DOI:10.1016/j.prp.2021.153347 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 7, 2021 Category: Pathology Authors: A Remo S Sina S Barbi I Simeone J Insolda P Parcesepe G Giordano L Cerulo M Ceccarelli F Fiorica A Bonetti M Pancione E Manfrin Source Type: research

The miR-187 induced bone reconstruction and healing in a mouse model of osteoporosis, and accelerated osteoblastic differentiation of human multipotent stromal cells by targeting BARX2
CONCLUSIONS: The miR-187 might have a significant therapeutic effect in osteoporotic fractures. miR-187 accelerated osteogenic differentiation of hMSCs by directly regulating BARX2.PMID:33550149 | DOI:10.1016/j.prp.2021.153340 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 7, 2021 Category: Pathology Authors: Jun Zhang Tao Zhang Bensen Tang Jing Li Zhengang Zha Source Type: research

Esophageal squamous cell carcinoma metachronous to head and neck cancers
CONCLUSION: Our study suggests that HPV infection is not an etiological factor associated to ESCC after HNSCC. On the other hand, p53 overexpression is a common finding. Moreover, our data suggest that an altered immune microenvironment, conditioned by a dysregulation in lymphomonocytic infiltration, may be a crucial factor allowing the occurrence of a metachronous ESCC.PMID:33545655 | DOI:10.1016/j.prp.2021.153346 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 5, 2021 Category: Pathology Authors: Gianluca Businello Matteo Fassan Silvia Degasperi Giulia Traverso Melania Scarpa Imerio Angriman Andromachi Kotsafti Ignazio Castagliuolo Marta Sbaraglia Romeo Bardini Marco Scarpa Source Type: research

Wnt5a: A promising therapeutic target in ovarian cancer
Pathol Res Pract. 2021 Jan 27;219:153348. doi: 10.1016/j.prp.2021.153348. Online ahead of print.ABSTRACTDespite the rapid development of novel adjuvant and neoadjuvant chemotherapeutic drugs in recent years, advanced ovarian cancer still lacks effective treatment strategies and remains one of the main causes of death among women. Wnt protein family is widely expressed in a variety of human tissues, and Wnt5a is an important member of the noncanonical Wnt pathway. Wnt5a has been found to induce tumor suppression as well as to function as an oncogene depending upon the specific cancer type. In ovarian cancer, Wnt5a protein e...
Source: Pathology, Research and Practice - February 4, 2021 Category: Pathology Authors: Weijian Zhou Jie Mei Dingyi Gu Junying Xu Runjie Wang Huiyu Wang Chaoying Liu Source Type: research

Gardnerella vaginalis and Trichomonas vaginalis infections as risk factors for persistence and progression of low-grade precancerous cervical lesions in HIV-1 positive women
In conclusion, GV infection is a risk factor for persistence of CIN1/L-SIL in HIV-positive women, while TV infection is a risk factor for progression. Women with these infections may require a closer and more careful follow-up of CIN1/L-SIL.PMID:33530017 | DOI:10.1016/j.prp.2021.153349 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 2, 2021 Category: Pathology Authors: Antonio Raffone Antonio Travaglino Antonio Angelino Rosanna Esposito Giuliana Orlandi Paolo Toscano Antonio Mollo Luigi Insabato Matilde Sansone Fulvio Zullo Source Type: research

Fibroblast function in COVID-19.
PMID: 33561763 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 1, 2021 Category: Pathology Authors: Sahin M, Akkus E Tags: Pathol Res Pract Source Type: research

Broad spectrum mutational analysis of chromophobe renal cell carcinoma using next-generation sequencing.
CONCLUSION: ChRCC is a rare disease still not fully molecularly characterized. Next-generation sequencing analysis could be useful to identify potential mutation with prognostic value or that could be potential therapeutic targets. PMID: 33556910 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 1, 2021 Category: Pathology Authors: Mollica V, Franceschini T, Gruppioni E, Rizzo A, Ricci C, Schiavina R, Brunocilla E, Ardizzoni A, Fiorentino M, Giunchi F, Massari F Tags: Pathol Res Pract Source Type: research

Wnt (canonical and non canonical) pathways in breast carcinoma with extensive vascular invasion and inflammatory breast carcinoma.
CONCLUSIONS: Canonical Wnt pathway is involved in malignant progression with dominant role for vascular invasion whereas non canonical Wnt pathway plays an important role on survival time including the capacity to identify carcinomas with IBC-like prognosis. Furthermore ePVI may represent a "prodromal form of IBC" as demonstrated by its clinicopathological and biological similarity with IBC. PMID: 33550148 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - January 27, 2021 Category: Pathology Authors: Remo A, Sina S, Barbi S, Simeone I, Insolda J, Parcesepe P, Giordano G, Cerulo L, Ceccarelli M, Fiorica F, Bonetti A, Pancione M, Manfrin E Tags: Pathol Res Pract Source Type: research

Esophageal squamous cell carcinoma metachronous to head and neck cancers.
CONCLUSION: Our study suggests that HPV infection is not an etiological factor associated to ESCC after HNSCC. On the other hand, p53 overexpression is a common finding. Moreover, our data suggest that an altered immune microenvironment, conditioned by a dysregulation in lymphomonocytic infiltration, may be a crucial factor allowing the occurrence of a metachronous ESCC. PMID: 33545655 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - January 27, 2021 Category: Pathology Authors: Businello G, Fassan M, Degasperi S, Traverso G, Scarpa M, Angriman I, Kotsafti A, Castagliuolo I, Sbaraglia M, Bardini R, Scarpa M Tags: Pathol Res Pract Source Type: research

Wnt5a: A promising therapeutic target in ovarian cancer.
Abstract Despite the rapid development of novel adjuvant and neoadjuvant chemotherapeutic drugs in recent years, advanced ovarian cancer still lacks effective treatment strategies and remains one of the main causes of death among women. Wnt protein family is widely expressed in a variety of human tissues, and Wnt5a is an important member of the noncanonical Wnt pathway. Wnt5a has been found to induce tumor suppression as well as to function as an oncogene depending upon the specific cancer type. In ovarian cancer, Wnt5a protein expression has been observed to be significantly upregulated and associated with poor p...
Source: Pathology, Research and Practice - January 27, 2021 Category: Pathology Authors: Zhou W, Mei J, Gu D, Xu J, Wang R, Wang H, Liu C Tags: Pathol Res Pract Source Type: research

Gardnerella vaginalis and Trichomonas vaginalis infections as risk factors for persistence and progression of low-grade precancerous cervical lesions in HIV-1 positive women.
In conclusion, GV infection is a risk factor for persistence of CIN1/L-SIL in HIV-positive women, while TV infection is a risk factor for progression. Women with these infections may require a closer and more careful follow-up of CIN1/L-SIL. PMID: 33530017 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - January 21, 2021 Category: Pathology Authors: Raffone A, Travaglino A, Angelino A, Esposito R, Orlandi G, Toscano P, Mollo A, Insabato L, Sansone M, Zullo F Tags: Pathol Res Pract Source Type: research

ZMYND8 promotes the growth and metastasis of hepatocellular carcinoma by promoting HK2-mediated glycolysis.
In this study, ZMYND8 expression was found to be elevated in HCC based on the cancer genome atlas (TCGA) and gene expression omnibus (GEO) databases. Next, we confirmed that ZMYND8 was frequently overexpressed in HCC tissues compared with adjacent non-tumor tissues. The up-regulated level of ZMYND8 was also observed in HCC cell lines. Elevated ZMYND8 expression was correlated with unfavorable clinicopathological features and poor prognosis of HCC patients. Functionally, ectopic expression of ZMYND8 potentiated the proliferation, migration, and invasion of Hep3B cells. Conversely, ZMYND8 knockdown led to the reduced prolife...
Source: Pathology, Research and Practice - January 19, 2021 Category: Pathology Authors: Dou C, Mo H, Chen T, Liu J, Zeng Y, Li S, Guo C, Zhang C Tags: Pathol Res Pract Source Type: research

An initiative to assess the quality of Tanzanian cervical cancer specimens for HPV and telomerase detection.
Abstract The aim of our study was to assess the quality of Tanzanian cervical cancer specimens, evaluating telomerase alterations and human papilloma virus (HPV) infection in relation to histopathological characteristics since these biomarkers are not routinely analyzed. Thirty-two Tanzanian women with invasive cervical cancer were included in the study. Histopathological classification and all the analyses on tissue, including TERT immunohistochemistry, were performed at IRST IRCCS (Meldola, Italy). HPV typization was performed by pyrosequencing. FHACT™ was used to identify chromosomal aberrations. Nonparam...
Source: Pathology, Research and Practice - January 18, 2021 Category: Pathology Authors: Ravaioli S, Pirini F, Tumedei MM, Puccetti M, Chiadini E, Serra P, Kahima J, Masalu N, Amadori D, Bravaccini S Tags: Pathol Res Pract Source Type: research

Colorectal polyposis as a clue to the diagnosis of Cowden syndrome: Report of two cases and literature review.
We report on two unrelated patients: a 46-year-old male and a 38-year-old woman, who were referred to the Genetic Clinic because of the endoscopic finding of multiple colorectal polyps. Despite both displayed striking clinical (and, in the first case, familial) manifestations of Cowden Syndrome (PTEN Hamartoma Tumor Syndrome-PHTS), they had not been recognized before. Diagnosis of PHTS was confirmed by the detection of causative PTEN variants. Pathological examination of the polyps showed multiple histology types: hyperplastic, juvenile, serrated and lymphoid. Hyperplastic polyps analyzed from both patients failed to show ...
Source: Pathology, Research and Practice - January 8, 2021 Category: Pathology Authors: Innella G, Miccoli S, Colussi D, Pradella LM, Amato LB, Zuntini R, Salfi NCM, Collina G, Ferrara F, Ricciardiello L, Turchetti D Tags: Pathol Res Pract Source Type: research

Expression profile of intestinal stem cell and cancer stem cell markers in gastric cancers with submucosal invasion.
Abstract Cancer stem cells (CSCs) are believed to be responsible for tumor growth, invasion, and metastasis. Submucosal invasion, which greatly enhances metastasis risk, is a critical step in gastric cancer (GC) progression. To identify stem cell-related markers associated with submucosal invasion and lymph node (LN) metastasis in GCs, we investigated the expression of candidate CSC markers (CD133, CD44, and ALDH1A) and intestinal stem cell (ISC) markers (EPHB2, OLFM4, and LGR5) in early GCs that manifested submucosal invasion. We discovered that EPHB2 and LGR5 expression was frequently confined to the basal area ...
Source: Pathology, Research and Practice - January 2, 2021 Category: Pathology Authors: Kim HS, Song HJ, Kim HU, Jeong IH, Koh HM, Shin JH, Jang BG Tags: Pathol Res Pract Source Type: research

PD-L1 expression in paired biopsies and surgical specimens in gastric adenocarcinoma: A digital image analysis study.
Abstract Programmed death-ligand 1 (PD-L1) expression in biopsies of gastric carcinoma may predict the results in corresponding surgical specimens. We compared PD-L1 immunohistochemistry (IHC) 22C3 pharmDx expression in paired biopsy and resection specimens. We also characterized the validity of a new PD-L1 assay using digital image analysis. PD-L1 IHC with 22C3 pharmDx and clone 73-10 was performed in 224 gastric cancer tissues (112 biopsies and paired surgical tissues) and the specimens were analyzed with the Leica Aperio Imagescope. For statistical analyses, the area under the receiver operating characteristic ...
Source: Pathology, Research and Practice - January 2, 2021 Category: Pathology Authors: Heo YJ, Kim B, Kim H, Kim S, Jang MS, Kim KM Tags: Pathol Res Pract Source Type: research

B7-H4 induces epithelial-mesenchymal transition and promotes colorectal cancer stemness.
Abstract B7-H4 is a unique negative regulator of T cells that is typically significantly overexpressed in various carcinomas and is associated with poor prognosis. However, the effects of B7-H4 expression on epithelial-mesenchymal transition (EMT) and cancer stemness of colorectal cancer (CRC) are not entirely clear. In the present study, we used tissue samples from 98 patients with CRC and CRC cell lines to determine the clinicopathological significance of B7-H4 in CRC and its effects on CRC stemness. We performed immunohistochemical staining; immunofluorescence imaging; western blotting; and tumor sphere formati...
Source: Pathology, Research and Practice - January 2, 2021 Category: Pathology Authors: Feng Y, Yang Z, Zhang C, Che N, Liu X, Xuan Y Tags: Pathol Res Pract Source Type: research

A methylation signature at the CpG island promoter of estrogen receptor beta (ER- β) in breasts of women may be an early footmark of lack of breastfeeding and nulliparity.
A methylation signature at the CpG island promoter of estrogen receptor beta (ER-β) in breasts of women may be an early footmark of lack of breastfeeding and nulliparity. Pathol Res Pract. 2020 Dec 28;218:153328 Authors: Daraei A, Izadi P, Khorasani G, Nafissi N, Naghizadeh MM, Meysamie A, Mansoori Y, Nariman-Saleh-Fam Z, Bastami M, Saadatian Z, Roshan SJ, Bayani N, Tavakkoly-Bazzaz J Abstract Although little is known regarding the mechanisms behind the onset of breast cancer (BC) through reproductive risk factors, new researches have highlighted some early tumor-related methylation footmarks in ...
Source: Pathology, Research and Practice - December 28, 2020 Category: Pathology Authors: Daraei A, Izadi P, Khorasani G, Nafissi N, Naghizadeh MM, Meysamie A, Mansoori Y, Nariman-Saleh-Fam Z, Bastami M, Saadatian Z, Roshan SJ, Bayani N, Tavakkoly-Bazzaz J Tags: Pathol Res Pract Source Type: research

MiR-216b regulates the tumorigenesis of gastric cancer by targeting PXN.
CONCLUSIONS: The above results showed that miR-216b could offer a novel therapeutic avenue by targeting PXN in GC. PMID: 33422779 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - December 28, 2020 Category: Pathology Authors: Liu X, Xu D, Xu X, Xue Q, Gao X, Tang C Tags: Pathol Res Pract Source Type: research

Emerging role of non-coding RNAs in response of cancer cells to radiotherapy.
Abstract Radiotherapy is an effective method for treatment of a large proportion of human cancers. Yet, the efficacy of this method is precluded by the induction of radioresistance in tumor cells and the radiation-associated injury of normal cells surrounding the field of radiation. These restrictions necessitate the introduction of modalities for either radiosensitization of cancer cells or protection of normal cells against adverse effects of radiation. Non-coding RNAs (ncRNAs) have essential roles in the determination of radiosensitivity. Moreover, ncRNAs can modulate radiation-induced side effects in normal ce...
Source: Pathology, Research and Practice - December 28, 2020 Category: Pathology Authors: Ebahimzadeh K, Shoorei H, Mousavinejad SA, Anamag FT, Dinger ME, Taheri M, Ghafouri-Fard S Tags: Pathol Res Pract Source Type: research

Immunotherapy of gastric cancer: Past, future perspective and challenges.
Abstract Gastric cancer is considered as the third leading cause of deaths and the fifth most common cancers worldwide. Common treatment approaches include chemotherapy, radiation, gastric resection and targeted therapies. The emergence of gastric cancer immunotherapy has already shown some promising results and have altered the therapeutic procedures. Now, different combination therapies as well as novel immunotherapies targeting new molecules have been proposed. Despite ongoing investigations on the therapeutic options and significant advancements in this regard, the disease is poorly prognosed. In fact, limited...
Source: Pathology, Research and Practice - December 24, 2020 Category: Pathology Authors: Xie J, Fu L, Jin L Tags: Pathol Res Pract Source Type: research

LncRNA polymorphisms and upper gastrointestinal cancer risk.
Abstract Upper gastrointestinal (GI) cancers such as oral (OC), esophageal (EC), and gastric (GC) cancers affect the digestive system, with a high mortality rate. Clinical symptoms are, however, not recognizable at early stages, and most patients are diagnosed in advanced stages. Therefore, the mechanism underlying the origin and development of upper GI cancer must be evaluated and also new therapeutic targets and effective methods should be identified and established to control GI cancers. Genome-wide approaches have introduced many long non-coding RNAs (lncRNAs) transcribed in various manners in malignant and no...
Source: Pathology, Research and Practice - December 24, 2020 Category: Pathology Authors: Abdi E, Latifi-Navid S, Latifi-Navid H, Safaralizadeh R Tags: Pathol Res Pract Source Type: research

Success or failure? Pathologists persecuted under National Socialism and their careers after emigrating to the United States.
This study examines the biographies of pathologists persecuted by the National Socialists after their emigration from the German Reich to the USA. The work is based on primary sources from various archives and a systematic evaluation of secondary literature on the persons concerned. The study yields five central results: (1) Out of 118 identified persecuted pathologists, a total of 91 persons left the German Reich, 60 of them demonstrably to the USA. (2) The majority of the pathologists immigrated to the USA between 1938 and 1941. (3) A good two thirds of the pathologists were (again) employed in the USA as university teac...
Source: Pathology, Research and Practice - December 14, 2020 Category: Pathology Authors: Uhlendahl H, Biermanns N, Sziranyi J, Groß D Tags: Pathol Res Pract Source Type: research

Quantitative polymerase Chain reaction profiling of microRNAs in peripheral lymph-monocytes from MGUS subjects.
This study aimed to evaluate microRNA expression on peripheral lymph-monocytes from MGUS subjects compared with healthy controls using qPCR arrays. Blood samples were collected by venipuncture from fifteen, newly diagnosed MGUS patients and fifteen healthy subjects. A further group (validation group) of six newly diagnosed MGUS patients and five healthy control were enrolled for the validation of miRNAs and their mRNAs target. The study was conducted performing miProfile miRNA qPCR arrays, followed by validation of miRNAs and related mRNA targets through RT-qPCR. The functional interaction between microRNAs and target gene...
Source: Pathology, Research and Practice - December 13, 2020 Category: Pathology Authors: Avenoso A, Campo S, Scuruchi M, Mania M, Innao V, D'Ascola A, Mandraffino G, Allegra AG, Musolino C, Allegra A Tags: Pathol Res Pract Source Type: research

Dihydroartemisinin inhibits the tumorigenesis and invasion of gastric cancer by regulating STAT1/KDR/MMP9 and P53/BCL2L1/CASP3/7 pathways.
In conclusion, DHA could suppress the tumorigenesis and invasion of GC by regulating STAT1/KDR/MMP9 and p53/BCL2L1/CASP3/7 pathways. Our findings might provide a novel approach for the treatment of GC. PMID: 33370709 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - December 13, 2020 Category: Pathology Authors: Liang R, Chen W, Chen XY, Fan HN, Zhang J, Zhu JS Tags: Pathol Res Pract Source Type: research

Diagnostic and prognostic role of NR3C4 in breast cancer through a genomic network understanding.
This study aimed to explore the expression, associations with clinicopathologic features and underlying molecular mechanisms of AR in breast cancer. The present study investigated invasive breast carcinoma through comprehensive bioinformatics. The expression and mutation rate of AR in breast cancer was obtained from the TCGA database. Training survival prediction analysis was applied to the data extracted from the KM plotter database. The prediction of the survival cohort was validated using the bcGenExMiner database in breast cancer molecular subgroups. Represented immunohistochemical images of AR and its related expressi...
Source: Pathology, Research and Practice - December 4, 2020 Category: Pathology Authors: Song Y, Huang R, Wu S, Zheng H, Guo M, Fu L, Yu S Tags: Pathol Res Pract Source Type: research

A new prognosis prediction model combining TNM stage with MAP2K4 and JNK in postoperative pancreatic cancer patients.
Abstract Mitogen-activated protein kinase kinase 4 (MAP2K4) is a tumor suppressor in many cancers. However, its roles and action mechanisms in pancreatic ductal adenocarcinoma (PDAC) remain unclear. Here, we analyzed MAP2K4 and its downstream kinases (c-Jun N-terminal kinase (JNK) and p38) using immunohistochemical staining and their prognostic significances using univariate and multivariate Cox proportional hazards regression analysis in our PDAC cohort. Then, we validated MAP2K4/JNK/p38 mRNA levels and prognostic significances using The Cancer Genome Atlas (TCGA) database. Finally, we evaluated the effects of MA...
Source: Pathology, Research and Practice - December 4, 2020 Category: Pathology Authors: Liu XD, Zhang ZW, Wu HW, Liang ZY Tags: Pathol Res Pract Source Type: research

Prevalence and spectrum of pathogenic germline variants in intestinal and pancreatobiliary type of ampullary cancer.
Abstract BACKGROUND: Ampullary cancer may occur as a component of hereditary cancer syndromes. Mutations in inherited cancer susceptibility genes play a therapeutic role and its knowledge in ampullary cancer is lacking. METHODS: Thirty-seven cases of ampullary carcinoma were subjected to tumor-normal whole exome sequencing with mean coverage of 100X (blood) and 200X (tumor). Data were analyzed and correlated with intestinal and pancreatobiliary differentiation. RESULTS: There were 22 intestinal, 13 pancreatobiliary and 2 cases of mixed differentiation. One hundred and forty-three germline variations with...
Source: Pathology, Research and Practice - December 4, 2020 Category: Pathology Authors: Kumari N, Singh RK, Mishra SK, L R, Mohindra S, Krishnani N Tags: Pathol Res Pract Source Type: research

The pivotal role of MicroRNAs in glucose metabolism in cancer.
Abstract Cancer cells are able to undergo aerobic glycolysis and metabolize glucose to lactate instead of oxidative phosphorylation, which is known as Warburg effect. Accumulating evidence has revealed that microRNAs regulate cancer cell metabolism, which manifest a higher rate of glucose metabolism. Various signaling pathways along with glycolytic enzymes are responsible for the emergence of glycolytic dependence. MicroRNAs are a class of non-coding RNAs that are not translated into proteins but regulate target gene expression or in other words function pre-translationally and post-transcriptionally. MicroRNAs ha...
Source: Pathology, Research and Practice - December 4, 2020 Category: Pathology Authors: Taefehshokr S, Taefehshokr N, Hemmat N, Hajazimian S, Isazadeh A, Dadebighlu P, Baradaran B Tags: Pathol Res Pract Source Type: research

Expression of CD117, CK17, CK20, MUC4, villin and mismatch repair deficiency in pancreatic intraductal papillary mucinous neoplasm.
MB Abstract Among pancreatic intraductal papillary neoplasms, gastric, intestinal, and pancreatobiliary intraductal papillary mucinous neoplasm (IPMN), intraductal oncocytic papillary neoplasm (IOPN), and intraductal tubulopapillary neoplasm (ITPN) have been defined, differing regarding association with invasive carcinoma and prognosis. Immunohistochemistry (IHC) can help in the distinction of these neoplasms, but a proportion is unclassifiable using recommended markers. Hence, additional markers useful for the typing of pancreatic intraductal papillary neoplasms are needed. The reported frequencies of the differ...
Source: Pathology, Research and Practice - December 4, 2020 Category: Pathology Authors: Detlefsen S, Jakobsen M, Nielsen MFB, Klöppel G, Mortensen MB Tags: Pathol Res Pract Source Type: research

Molecular and immunohistochemical evaluation of BAP-1 antibody in bladder cancer and comparison with luminal-basal subtyping.
CONCLUSIONS: Molecular subtyping has become increasingly important in bladder cancer and it is mainly divided into "luminal" and "basal" type. Despite the large amount of studies about molecular pathway of the bladder cancer, there are a few studies about BAP-1. The aim of this study is to evaluate the BAP-1 expression molecularly and immunohistochemically and compare it with GATA-3 and CK5/6 immunohistochemical stains. PMID: 33341088 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - December 4, 2020 Category: Pathology Authors: Gürbüz BÇ, Topal CS, Sobay R, Alkurt G, Zemheri IE Tags: Pathol Res Pract Source Type: research