Poorly differentiated neuroendocrine carcinomas of the gastrointestinal tract: A single-institute study of 43 cases
Pathol Res Pract. 2021 Sep 11;226:153614. doi: 10.1016/j.prp.2021.153614. Online ahead of print.ABSTRACTOBJECTIVES: To characterize the clinicopathologic and immunohistochemical features of poorly differentiated neuroendocrine carcinomas (NEC) in the gastrointestinal tract.DESIGN: A total of 43 cases were identified and reassessed based on modern classification.RESULTS: The cohort (27M, 16F; median age: 66 years) included 16 (37%) large cell NEC, 12 (28%) small cell NEC, 5 (12%) NEC not otherwise specified, and 10 (23%) mixed adenoneuroendocrine carcinomas. Tumor predominantly involved the colon (n = 14, 33%), rectum (n = ...
Source: Pathology, Research and Practice - September 23, 2021 Category: Pathology Authors: Irene Chen Dongwei Zhang Moises Velez Sierra Kovar Xiaoyan Liao Source Type: research

Role of GATA3 in tumor diagnosis: A review
Pathol Res Pract. 2021 Sep 13;226:153611. doi: 10.1016/j.prp.2021.153611. Online ahead of print.ABSTRACTGATA binding protein 3 (GATA3) belongs to a family of transcription factors comprising six members. These proteins identify G-A-T-A containing sequences in the target gene and bind to DNA target via two zinc-finger domains. The aim of this study was to evaluate the role of GATA3 in the diagnosis of tumors and its value as a prognostic marker. To perform this review, a comprehensive search was conducted through PubMed, Embase, Scopus, Cochrane and Google Scholar databases from 1985 to 2020. Articles were considered thorou...
Source: Pathology, Research and Practice - September 21, 2021 Category: Pathology Authors: Mahdis Khazaeli Najafabadi Elham Mirzaeian Sahar Memar Montazerin Amir Reza Tavangar Mohammadreza Tabary Seyed Mohammad Tavangar Source Type: research

Role of GATA3 in tumor diagnosis: A review
Pathol Res Pract. 2021 Sep 13;226:153611. doi: 10.1016/j.prp.2021.153611. Online ahead of print.ABSTRACTGATA binding protein 3 (GATA3) belongs to a family of transcription factors comprising six members. These proteins identify G-A-T-A containing sequences in the target gene and bind to DNA target via two zinc-finger domains. The aim of this study was to evaluate the role of GATA3 in the diagnosis of tumors and its value as a prognostic marker. To perform this review, a comprehensive search was conducted through PubMed, Embase, Scopus, Cochrane and Google Scholar databases from 1985 to 2020. Articles were considered thorou...
Source: Pathology, Research and Practice - September 21, 2021 Category: Pathology Authors: Mahdis Khazaeli Najafabadi Elham Mirzaeian Sahar Memar Montazerin Amir Reza Tavangar Mohammadreza Tabary Seyed Mohammad Tavangar Source Type: research

Role of GATA3 in tumor diagnosis: A review
Pathol Res Pract. 2021 Sep 13;226:153611. doi: 10.1016/j.prp.2021.153611. Online ahead of print.ABSTRACTGATA binding protein 3 (GATA3) belongs to a family of transcription factors comprising six members. These proteins identify G-A-T-A containing sequences in the target gene and bind to DNA target via two zinc-finger domains. The aim of this study was to evaluate the role of GATA3 in the diagnosis of tumors and its value as a prognostic marker. To perform this review, a comprehensive search was conducted through PubMed, Embase, Scopus, Cochrane and Google Scholar databases from 1985 to 2020. Articles were considered thorou...
Source: Pathology, Research and Practice - September 21, 2021 Category: Pathology Authors: Mahdis Khazaeli Najafabadi Elham Mirzaeian Sahar Memar Montazerin Amir Reza Tavangar Mohammadreza Tabary Seyed Mohammad Tavangar Source Type: research

Autophagy in cardiac myxoma: An important puzzle piece in understanding its inflammatory environment
CONCLUSION: Our data not only confirm the presence of autophagic markers within cardiac myxomas but also suggest a possible association with their immune microenvironment.PMID:34537518 | DOI:10.1016/j.prp.2021.153609 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - September 19, 2021 Category: Pathology Authors: Viviana Sramek Maroa Dridi Alexandra Papoudou-Bai Jean Marc Dumollard Michel P éoc'h Georgia Karpathiou Source Type: research

Autophagy in cardiac myxoma: An important puzzle piece in understanding its inflammatory environment
CONCLUSION: Our data not only confirm the presence of autophagic markers within cardiac myxomas but also suggest a possible association with their immune microenvironment.PMID:34537518 | DOI:10.1016/j.prp.2021.153609 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - September 19, 2021 Category: Pathology Authors: Viviana Sramek Maroa Dridi Alexandra Papoudou-Bai Jean Marc Dumollard Michel P éoc'h Georgia Karpathiou Source Type: research

Can evaluation of mismatch repair defect and TILs increase the number of triple-negative breast cancer patients eligible for immunotherapy?
CONCLUSIONS: A substantial proportion of PD-L1 negative, TNBC patients showed complete/partial loss of MMR and/or high TILs indicating that supplementing PD-L1 examination with these biomarkers may identify TNBC-patients that may be selected for immunotherapy.PMID:34530255 | DOI:10.1016/j.prp.2021.153606 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - September 16, 2021 Category: Pathology Authors: Demet Özcan Johanne Lade-Keller Trine Tramm Source Type: research

Can histologic features predict neoadjuvant therapy response in rectal adenocarcinoma?
This study aimed to identify histologic features that predict tumor response. This retrospective study included 105 patients with LARC, all of whom underwent biopsy followed by neoadjuvant therapy and subsequent surgical resection. Each patient's initial biopsy was evaluated for tumor grade, tumor budding, intraepithelial lymphocytes, intraepithelial neutrophils, desmoplasia, apoptosis, adjacent stromal lymphocytes, signet ring cells, mucinous features, tumoral Paneth cells, dirty necrosis, microscopic ulceration, and prominent lymphoid aggregates. These histologic features, along with patient age at diagnosis and tumor mi...
Source: Pathology, Research and Practice - September 16, 2021 Category: Pathology Authors: Yuho Ono Justin M M Cates Raul S Gonzalez Source Type: research

PD-L1 evaluation in head and neck squamous cell carcinoma: Insights regarding specimens, heterogeneity and therapy
Pathol Res Pract. 2021 Sep 1;226:153605. doi: 10.1016/j.prp.2021.153605. Online ahead of print.ABSTRACTImmunohistochemical assessment with combined positive score (CPS) of programmed death-ligand 1 (PD-L1) is the prerequisite for administration of checkpoint inhibitor therapy in head and neck squamous cell carcinoma (HNSCC). Practicing pathologists are required to assess PD-L1 in routinary work and can be faced up with practical issues not always addressed in clinical trials or guidelines, such as choice of specimen to test, the intrinsic heterogeneity in PD-L1 expression in tumors and the potential impact of already admin...
Source: Pathology, Research and Practice - September 16, 2021 Category: Pathology Authors: Gaetano Paolino Liron Pantanowitz Valeria Barresi Fabio Pagni Enrico Munari Lorenzo Moretta Matteo Brunelli Elena Bariani Elena Vigliar Pasquale Pisapia Umberto Malapelle Giancarlo Troncone Ilaria Girolami Albino Eccher Source Type: research

Development of a multiplex immuno-oncology biomarker and digital pathology workflow for assessment of urothelial carcinoma
CONCLUSIONS: m-IHC offers significant advantages in characterizing the host immune microenvironment particularly in limited biopsy tissue material. Utilizing a digital image workflow resulted in significant concordance between m-IHC and individual single stains (p
Source: Pathology, Research and Practice - September 11, 2021 Category: Pathology Authors: Youheng Xie Ekaterina Olkhov-Mitsel Samira Alminawi Elzbieta Slodkowska Michelle R Downes Source Type: research

Development of a multiplex immuno-oncology biomarker and digital pathology workflow for assessment of urothelial carcinoma
CONCLUSIONS: m-IHC offers significant advantages in characterizing the host immune microenvironment particularly in limited biopsy tissue material. Utilizing a digital image workflow resulted in significant concordance between m-IHC and individual single stains (p
Source: Pathology, Research and Practice - September 11, 2021 Category: Pathology Authors: Youheng Xie Ekaterina Olkhov-Mitsel Samira Alminawi Elzbieta Slodkowska Michelle R Downes Source Type: research

Development of a multiplex immuno-oncology biomarker and digital pathology workflow for assessment of urothelial carcinoma
CONCLUSIONS: m-IHC offers significant advantages in characterizing the host immune microenvironment particularly in limited biopsy tissue material. Utilizing a digital image workflow resulted in significant concordance between m-IHC and individual single stains (p
Source: Pathology, Research and Practice - September 11, 2021 Category: Pathology Authors: Youheng Xie Ekaterina Olkhov-Mitsel Samira Alminawi Elzbieta Slodkowska Michelle R Downes Source Type: research

Development of a multiplex immuno-oncology biomarker and digital pathology workflow for assessment of urothelial carcinoma
CONCLUSIONS: m-IHC offers significant advantages in characterizing the host immune microenvironment particularly in limited biopsy tissue material. Utilizing a digital image workflow resulted in significant concordance between m-IHC and individual single stains (p
Source: Pathology, Research and Practice - September 11, 2021 Category: Pathology Authors: Youheng Xie Ekaterina Olkhov-Mitsel Samira Alminawi Elzbieta Slodkowska Michelle R Downes Source Type: research

Development of a multiplex immuno-oncology biomarker and digital pathology workflow for assessment of urothelial carcinoma
CONCLUSIONS: m-IHC offers significant advantages in characterizing the host immune microenvironment particularly in limited biopsy tissue material. Utilizing a digital image workflow resulted in significant concordance between m-IHC and individual single stains (p
Source: Pathology, Research and Practice - September 11, 2021 Category: Pathology Authors: Youheng Xie Ekaterina Olkhov-Mitsel Samira Alminawi Elzbieta Slodkowska Michelle R Downes Source Type: research

Development of a multiplex immuno-oncology biomarker and digital pathology workflow for assessment of urothelial carcinoma
CONCLUSIONS: m-IHC offers significant advantages in characterizing the host immune microenvironment particularly in limited biopsy tissue material. Utilizing a digital image workflow resulted in significant concordance between m-IHC and individual single stains (p
Source: Pathology, Research and Practice - September 11, 2021 Category: Pathology Authors: Youheng Xie Ekaterina Olkhov-Mitsel Samira Alminawi Elzbieta Slodkowska Michelle R Downes Source Type: research

Development of a multiplex immuno-oncology biomarker and digital pathology workflow for assessment of urothelial carcinoma
CONCLUSIONS: m-IHC offers significant advantages in characterizing the host immune microenvironment particularly in limited biopsy tissue material. Utilizing a digital image workflow resulted in significant concordance between m-IHC and individual single stains (p
Source: Pathology, Research and Practice - September 11, 2021 Category: Pathology Authors: Youheng Xie Ekaterina Olkhov-Mitsel Samira Alminawi Elzbieta Slodkowska Michelle R Downes Source Type: research

Tissue-derived extracellular vesicles: Research progress from isolation to application
Pathol Res Pract. 2021 Aug 30;226:153604. doi: 10.1016/j.prp.2021.153604. Online ahead of print.ABSTRACTExtracellular vesicles (EVs) are the structures that all cells release into the environment. They are separated by a lipid bilayer and contain the cellular components that release them. To date, most studies have been performed on EVs derived from cell supernatants or different body fluids, while the number of studies on EV isolation directly from tissues is still limited. Studies of EV isolation directly from tissues may provide us with better information. This review summarizes the role of EV in the extracellular matri...
Source: Pathology, Research and Practice - September 9, 2021 Category: Pathology Authors: Bo Qin Xi-Min Hu Zhen-Hong Su Xiao-Bo Zeng Hong-Ying Ma Kun Xiong Source Type: research

LncRNA RP11-116G8.5 promotes the progression of lung squamous cell carcinoma through sponging miR-3150b-3p/miR-6870-5p to upregulate PHF12/FOXP4
CONCLUSIONS: LncRNA RP11-116G8.5 promotes malignant behaviors of LUSC through sponging miR-3150b-3p/miR-6870-5p to upregulate PHF12/FOXP4 expression.AVAILABILITY OF DATA: The research data is confidential.PMID:34500373 | DOI:10.1016/j.prp.2021.153566 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - September 9, 2021 Category: Pathology Authors: Hongqing Li Qing Zhao Zhonghao Tang Source Type: research

TOPK: A new predictor of the therapeutic response to neoadjuvant chemotherapy and prognosis in triple-negative breast cancer
CONCLUSION: TOPK may be a novel predictor for the therapeutic response to NACT and prognosis for patients with TNBC.PMID:34500374 | DOI:10.1016/j.prp.2021.153603 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - September 9, 2021 Category: Pathology Authors: Kaijing Wang Jia Chai Junpeng Xu Jie Wei Peifeng Li Yixiong Liu Jing Ma Tianqi Xu Danhui Zhao Kangjie Yu Linni Fan Qingguo Yan Shuangping Guo Mingyang Li Zhe Wang Source Type: research

Tissue-derived extracellular vesicles: Research progress from isolation to application
Pathol Res Pract. 2021 Aug 30;226:153604. doi: 10.1016/j.prp.2021.153604. Online ahead of print.ABSTRACTExtracellular vesicles (EVs) are the structures that all cells release into the environment. They are separated by a lipid bilayer and contain the cellular components that release them. To date, most studies have been performed on EVs derived from cell supernatants or different body fluids, while the number of studies on EV isolation directly from tissues is still limited. Studies of EV isolation directly from tissues may provide us with better information. This review summarizes the role of EV in the extracellular matri...
Source: Pathology, Research and Practice - September 9, 2021 Category: Pathology Authors: Bo Qin Xi-Min Hu Zhen-Hong Su Xiao-Bo Zeng Hong-Ying Ma Kun Xiong Source Type: research

LncRNA RP11-116G8.5 promotes the progression of lung squamous cell carcinoma through sponging miR-3150b-3p/miR-6870-5p to upregulate PHF12/FOXP4
CONCLUSIONS: LncRNA RP11-116G8.5 promotes malignant behaviors of LUSC through sponging miR-3150b-3p/miR-6870-5p to upregulate PHF12/FOXP4 expression.AVAILABILITY OF DATA: The research data is confidential.PMID:34500373 | DOI:10.1016/j.prp.2021.153566 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - September 9, 2021 Category: Pathology Authors: Hongqing Li Qing Zhao Zhonghao Tang Source Type: research

TOPK: A new predictor of the therapeutic response to neoadjuvant chemotherapy and prognosis in triple-negative breast cancer
CONCLUSION: TOPK may be a novel predictor for the therapeutic response to NACT and prognosis for patients with TNBC.PMID:34500374 | DOI:10.1016/j.prp.2021.153603 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - September 9, 2021 Category: Pathology Authors: Kaijing Wang Jia Chai Junpeng Xu Jie Wei Peifeng Li Yixiong Liu Jing Ma Tianqi Xu Danhui Zhao Kangjie Yu Linni Fan Qingguo Yan Shuangping Guo Mingyang Li Zhe Wang Source Type: research

The role of glycometabolic plasticity in cancer
Pathol Res Pract. 2021 Aug 25;226:153595. doi: 10.1016/j.prp.2021.153595. Online ahead of print.ABSTRACTDysregulated glycometabolism represented by the Warburg effect is well recognized as a hallmark of cancer that can be driven by oncogenes (e.g., c-Myc, K-ras, and BRAF) and contribute to cellular malignant transformation. The Warburg effect reveals the different glycometabolic patterns of cancer cells, but this unique glycometabolic pattern has the characteristic of plasticity rather than changeless which can vary with different internal or external stimuli during evolution. Glycometabolic plasticity enables cancer cells...
Source: Pathology, Research and Practice - September 4, 2021 Category: Pathology Authors: Yuyang Peng Hui Yang Song Li Source Type: research

Bcl-10, trypsin and synaptophysin helps recognize acinar cell and mixed acinar neuroendocrine cell carcinoma of the pancreas on both preoperative cytological samples and needle biopsy specimens
CONCLUSIONS: BCL10 is a determinant marker for the diagnosis of acinar cell carcinoma and mixed acinar neuroendocrine cell carcinoma of the pancreas in a pre-operative citologic/histologic setting.PMID:34481211 | DOI:10.1016/j.prp.2021.153593 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - September 4, 2021 Category: Pathology Authors: Erminia Manfrin Alice Parisi Lavinia Stefanizzi Mirko D'Onofrio Laura Bernardoni Stefano Francesco Crino Giuseppe Pelosi Massimo Pancione Guido Giordano Sokol Sina Andrea Remo Source Type: research

p53, p16 and ki67 as immunohistochemical prognostic markers in uterine smooth muscle tumors of uncertain malignant potential (STUMP)
In conclusion, p53 and p16 might be useful in the risk assessment of STUMP, despite not being suitable as stand-alone prognostic markers.PMID:34481212 | DOI:10.1016/j.prp.2021.153592 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - September 4, 2021 Category: Pathology Authors: Antonio Travaglino Antonio Raffone Annarita Gencarelli Daniele Neola Domenico Alessandro Oliviero Rosa Alfano Maria Raffaela Campanino Federica Cariati Fulvio Zullo Antonio Mollo Luigi Insabato Source Type: research

A review on the role of GAS6 and GAS6-AS1 in the carcinogenesis
Pathol Res Pract. 2021 Aug 25;226:153596. doi: 10.1016/j.prp.2021.153596. Online ahead of print.ABSTRACTGrowth arrest specific 6 (GAS6) encodes a protein that serves as a ligand for AXL receptor tyrosine kinase and stimulates cell proliferation. Notably, an antisense RNA, namely GAS6-AS1 is transcribed from chromosome 13q34, near GAS6 gene. In vitro functional experiments have demonstrated that GAS6-AS1 can promote proliferation, migration and invasive properties of transformed cells through enhancing entry into S-phase. Notably, mechanistic investigations have shown that GAS6-AS1 can regulate expression of GAS6 at the tra...
Source: Pathology, Research and Practice - September 4, 2021 Category: Pathology Authors: Soudeh Ghafouri-Fard Tayyebeh Khoshbakht Mohammad Taheri Majid Mokhtari Source Type: research

Malignant melanoma of the prostate gland: A systematic review
Pathol Res Pract. 2021 Aug 24;226:153594. doi: 10.1016/j.prp.2021.153594. Online ahead of print.ABSTRACTAIMS: Prostatic melanoma is a rare malignancy about which only scattered case reports and no systematic reviews have been published to date. We sought to better inform clinicians and pathologists caring for these patients by gathering all available evidence on the topic.METHODS: We performed a systematic review of English and non-English articles indexed in PubMed, EMBASE, Scopus and Google Scholar about primary and metastatic prostatic melanoma.RESULTS: In total, 25 studies describing 45 cases were identified. Most case...
Source: Pathology, Research and Practice - September 4, 2021 Category: Pathology Authors: Alessandro Caputo Maria Addesso Pio Zeppa Antonio D'Antonio Source Type: research

The role of glycometabolic plasticity in cancer
Pathol Res Pract. 2021 Aug 25;226:153595. doi: 10.1016/j.prp.2021.153595. Online ahead of print.ABSTRACTDysregulated glycometabolism represented by the Warburg effect is well recognized as a hallmark of cancer that can be driven by oncogenes (e.g., c-Myc, K-ras, and BRAF) and contribute to cellular malignant transformation. The Warburg effect reveals the different glycometabolic patterns of cancer cells, but this unique glycometabolic pattern has the characteristic of plasticity rather than changeless which can vary with different internal or external stimuli during evolution. Glycometabolic plasticity enables cancer cells...
Source: Pathology, Research and Practice - September 4, 2021 Category: Pathology Authors: Yuyang Peng Hui Yang Song Li Source Type: research

Bcl-10, trypsin and synaptophysin helps recognize acinar cell and mixed acinar neuroendocrine cell carcinoma of the pancreas on both preoperative cytological samples and needle biopsy specimens
CONCLUSIONS: BCL10 is a determinant marker for the diagnosis of acinar cell carcinoma and mixed acinar neuroendocrine cell carcinoma of the pancreas in a pre-operative citologic/histologic setting.PMID:34481211 | DOI:10.1016/j.prp.2021.153593 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - September 4, 2021 Category: Pathology Authors: Erminia Manfrin Alice Parisi Lavinia Stefanizzi Mirko D'Onofrio Laura Bernardoni Stefano Francesco Crino Giuseppe Pelosi Massimo Pancione Guido Giordano Sokol Sina Andrea Remo Source Type: research

p53, p16 and ki67 as immunohistochemical prognostic markers in uterine smooth muscle tumors of uncertain malignant potential (STUMP)
In conclusion, p53 and p16 might be useful in the risk assessment of STUMP, despite not being suitable as stand-alone prognostic markers.PMID:34481212 | DOI:10.1016/j.prp.2021.153592 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - September 4, 2021 Category: Pathology Authors: Antonio Travaglino Antonio Raffone Annarita Gencarelli Daniele Neola Domenico Alessandro Oliviero Rosa Alfano Maria Raffaela Campanino Federica Cariati Fulvio Zullo Antonio Mollo Luigi Insabato Source Type: research

A review on the role of GAS6 and GAS6-AS1 in the carcinogenesis
Pathol Res Pract. 2021 Aug 25;226:153596. doi: 10.1016/j.prp.2021.153596. Online ahead of print.ABSTRACTGrowth arrest specific 6 (GAS6) encodes a protein that serves as a ligand for AXL receptor tyrosine kinase and stimulates cell proliferation. Notably, an antisense RNA, namely GAS6-AS1 is transcribed from chromosome 13q34, near GAS6 gene. In vitro functional experiments have demonstrated that GAS6-AS1 can promote proliferation, migration and invasive properties of transformed cells through enhancing entry into S-phase. Notably, mechanistic investigations have shown that GAS6-AS1 can regulate expression of GAS6 at the tra...
Source: Pathology, Research and Practice - September 4, 2021 Category: Pathology Authors: Soudeh Ghafouri-Fard Tayyebeh Khoshbakht Mohammad Taheri Majid Mokhtari Source Type: research

Malignant melanoma of the prostate gland: A systematic review
Pathol Res Pract. 2021 Aug 24;226:153594. doi: 10.1016/j.prp.2021.153594. Online ahead of print.ABSTRACTAIMS: Prostatic melanoma is a rare malignancy about which only scattered case reports and no systematic reviews have been published to date. We sought to better inform clinicians and pathologists caring for these patients by gathering all available evidence on the topic.METHODS: We performed a systematic review of English and non-English articles indexed in PubMed, EMBASE, Scopus and Google Scholar about primary and metastatic prostatic melanoma.RESULTS: In total, 25 studies describing 45 cases were identified. Most case...
Source: Pathology, Research and Practice - September 4, 2021 Category: Pathology Authors: Alessandro Caputo Maria Addesso Pio Zeppa Antonio D'Antonio Source Type: research

Primary urothelial carcinoma of the ureter without concurrent renal pelvic or bladder carcinoma: A contemporary clinicopathologic analysis
Pathol Res Pract. 2021 Aug 13;226:153584. doi: 10.1016/j.prp.2021.153584. Online ahead of print.ABSTRACTPrimary urothelial carcinoma (UCa) of the ureter is relatively uncommon, comprising less than 10% of all urinary tract tumors. Typically, ureteral UCa is found in association with other urinary tract tumors, such as renal pelvic or bladder UCa, making it challenging to analyze the clinicopathologic features in isolation. With only a few small case series and case reports available, our understanding of primary ureteral UCa is limited. Herein, we conducted one of the largest studies to date of primary ureteral UCa without...
Source: Pathology, Research and Practice - August 30, 2021 Category: Pathology Authors: Christina M Gutierrez Bradley C Carthon Shreyas Joshi Adeboye O Osunkoya Source Type: research

Benign myofibroblastic/epithelial lesions of the breast with minimally infiltrative margins
This study emphasizes the clinico-pathologic features of 5 cases of mammary benign fibro(stromo)-epithelial lesions sharing as a common morphologic theme the presence of minimally infiltrative margins due to the overgrowth of the stromal component into the surrounding fibro-fatty tissue. Notably the radiological features were suspicious for malignancy in 3 out of 5 cases. The following histologic features were seen in all cases: i) an epithelial component of benign glandular structures exhibiting the morphological spectrum of fibro-cystic disease; ii) a stromal component of bland-looking spindle cell myofibroblasts (viment...
Source: Pathology, Research and Practice - August 29, 2021 Category: Pathology Authors: Giada Maria Vecchio Giuseppe Angelico Giuseppe Broggi Valentina Virz ì Lucia Salvatorelli Gaetano Magro Source Type: research

The histological and molecular spectrum of lipoblastoma: A case series with identification of three novel gene fusions by targeted RNA-sequencing
Pathol Res Pract. 2021 Aug 18;226:153591. doi: 10.1016/j.prp.2021.153591. Online ahead of print.ABSTRACTLipoblastoma is a rare benign mesenchymal neoplasm that typically occurs in infancy but may also occur in older age groups and various locations. Thus, there are often numerous clinical differential diagnoses. Moreover, lipoblastomas can show a broad histologic spectrum, which can hamper the correct diagnosis, particularly in small biopsies. At the genomic level, lipoblastomas are characterized by chromosomal fusions involving the PLAG1 gene. We investigated 11 lipoblastoma samples from 10 pediatric patients (age range f...
Source: Pathology, Research and Practice - August 29, 2021 Category: Pathology Authors: Elena Gerhard-Hartmann Christian Vokuhl Sabine Roth Tabea Steinm üller Mathias Rosenfeldt Alberto Zam ò Andreas Rosenwald Silke Appenzeller Karen Ernestus Katja Maurus Source Type: research

Usefulness of high-risk human papillomavirus mRNA silver in situ hybridization diagnostic assay  in oropharyngeal squamous cell carcinomas
CONCLUSIONS: The recently developed mRNA SISH methodology can detect HPV-driven OPSCCs without any additional test in 79% of cases. Positive SISH signals enable the visualization of viral transcripts required to recognize clinically relevant HPV infection. However, rare and tiny signals require an experienced pathologist to establish a consensus interpretation of results. The currently applied HR-HPV mRNA SISH analysis may serve as a groundwork for additional studies.PMID:34455364 | DOI:10.1016/j.prp.2021.153585 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - August 29, 2021 Category: Pathology Authors: Nina Gale Mario Poljak Metka Volav šek Lea Ho šnjak Dane Velkavrh Luka Bolha Kristina Fujs Komlo š Primo ž Strojan Aleksandar Ani čin Nina Zidar Source Type: research

Breast implant-associated EBV-positive diffuse large B-cell lymphoma: Two case reports and literature review
Pathol Res Pract. 2021 Aug 13;226:153589. doi: 10.1016/j.prp.2021.153589. Online ahead of print.ABSTRACTLymphomas associated with breast implants are mostly of the T-cell type. They are predominantly anaplastic lymphoma kinase-negative anaplastic large cell lymphoma (ALK-negative ALCL) characterized by CD30 positivity universally. Whilst the majority of primary breast lymphomas occurring in the absence of breast implants are of B-cell origin, there are few cases of implant-associated B-cell lymphomas reported to date in the literature, a subset of which are diffuse large B-cell lymphoma (DLBCL). Given the rarity of this en...
Source: Pathology, Research and Practice - August 29, 2021 Category: Pathology Authors: Sarah Morgan Rosemarie Tremblay-LeMay Joan E Lipa Monalisa Sur Jan Delabie Kevin Imrie Michael Crump Laura J Snell Zeina Ghorab Source Type: research

Benign myofibroblastic/epithelial lesions of the breast with minimally infiltrative margins
This study emphasizes the clinico-pathologic features of 5 cases of mammary benign fibro(stromo)-epithelial lesions sharing as a common morphologic theme the presence of minimally infiltrative margins due to the overgrowth of the stromal component into the surrounding fibro-fatty tissue. Notably the radiological features were suspicious for malignancy in 3 out of 5 cases. The following histologic features were seen in all cases: i) an epithelial component of benign glandular structures exhibiting the morphological spectrum of fibro-cystic disease; ii) a stromal component of bland-looking spindle cell myofibroblasts (viment...
Source: Pathology, Research and Practice - August 29, 2021 Category: Pathology Authors: Giada Maria Vecchio Giuseppe Angelico Giuseppe Broggi Valentina Virz ì Lucia Salvatorelli Gaetano Magro Source Type: research

The histological and molecular spectrum of lipoblastoma: A case series with identification of three novel gene fusions by targeted RNA-sequencing
Pathol Res Pract. 2021 Aug 18;226:153591. doi: 10.1016/j.prp.2021.153591. Online ahead of print.ABSTRACTLipoblastoma is a rare benign mesenchymal neoplasm that typically occurs in infancy but may also occur in older age groups and various locations. Thus, there are often numerous clinical differential diagnoses. Moreover, lipoblastomas can show a broad histologic spectrum, which can hamper the correct diagnosis, particularly in small biopsies. At the genomic level, lipoblastomas are characterized by chromosomal fusions involving the PLAG1 gene. We investigated 11 lipoblastoma samples from 10 pediatric patients (age range f...
Source: Pathology, Research and Practice - August 29, 2021 Category: Pathology Authors: Elena Gerhard-Hartmann Christian Vokuhl Sabine Roth Tabea Steinm üller Mathias Rosenfeldt Alberto Zam ò Andreas Rosenwald Silke Appenzeller Karen Ernestus Katja Maurus Source Type: research

Usefulness of high-risk human papillomavirus mRNA silver in situ hybridization diagnostic assay  in oropharyngeal squamous cell carcinomas
CONCLUSIONS: The recently developed mRNA SISH methodology can detect HPV-driven OPSCCs without any additional test in 79% of cases. Positive SISH signals enable the visualization of viral transcripts required to recognize clinically relevant HPV infection. However, rare and tiny signals require an experienced pathologist to establish a consensus interpretation of results. The currently applied HR-HPV mRNA SISH analysis may serve as a groundwork for additional studies.PMID:34455364 | DOI:10.1016/j.prp.2021.153585 (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - August 29, 2021 Category: Pathology Authors: Nina Gale Mario Poljak Metka Volav šek Lea Ho šnjak Dane Velkavrh Luka Bolha Kristina Fujs Komlo š Primo ž Strojan Aleksandar Ani čin Nina Zidar Source Type: research

Breast implant-associated EBV-positive diffuse large B-cell lymphoma: Two case reports and literature review
Pathol Res Pract. 2021 Aug 13;226:153589. doi: 10.1016/j.prp.2021.153589. Online ahead of print.ABSTRACTLymphomas associated with breast implants are mostly of the T-cell type. They are predominantly anaplastic lymphoma kinase-negative anaplastic large cell lymphoma (ALK-negative ALCL) characterized by CD30 positivity universally. Whilst the majority of primary breast lymphomas occurring in the absence of breast implants are of B-cell origin, there are few cases of implant-associated B-cell lymphomas reported to date in the literature, a subset of which are diffuse large B-cell lymphoma (DLBCL). Given the rarity of this en...
Source: Pathology, Research and Practice - August 29, 2021 Category: Pathology Authors: Sarah Morgan Rosemarie Tremblay-LeMay Joan E Lipa Monalisa Sur Jan Delabie Kevin Imrie Michael Crump Laura J Snell Zeina Ghorab Source Type: research

Synchronous detection of pancreatic adenocarcinoma and paraganglioma in a Whipple resection specimen
We report a case of a pancreatic ductal adenocarcinoma (PDAC) presenting synchronously with a paraganglioma (PGL) in a Whipple reaction specimen. The patient was a 72-year-old female with a history of breast and vulvar cancer. The simultaneous occurrence of two synchronous tumours in the pancreas was striking. Due to the presence of PGL and multiple meta- and synchronous tumours, the patient was referred to genetic counselling. Tumour tissue from the vulvar carcinoma, the PDAC and the PGL was analysed by targeted next-generation sequencing (NGS) of 161 cancer-related genes and by whole exome sequencing (WES). Peripheral bl...
Source: Pathology, Research and Practice - August 28, 2021 Category: Pathology Authors: Trine Aaquist Maja Dembic Mads Thomassen Karin de Stricker Mette Bertelsen Lene Gaarsmand Christensen Michael Bau Mortensen S önke Detlefsen Source Type: research

New evidence on tumor suppressor activity of PTEN and KLLN in papillary thyroid carcinoma
This study aimed to address the hypothesis that the expression of PTEN and KLLN tumor suppressor genes could diminish in papillary thyroid cancer (PTC) compared to paired normal tissue (PNT) and multinodular goiter (MNG). PTEN and KLLN expressions were assessed at both mRNA and protein levels in 82 tissue samples, including 30 PTC, 30 PNT, and 26 MNG using SYBR-Green Real-Time PCR and enzyme-linked immunosorbent assay (ELISA), respectively. Bioinformatics studies were performed to evaluate the genomic location and the genes promoter region. The mRNA expression of PTEN and KLLN in PTC was significantly lower than PNT (PTEN,...
Source: Pathology, Research and Practice - August 23, 2021 Category: Pathology Authors: S Adeleh Razavi Pouya Salehipour Hanieh Gholami Sara Sheikholeslami Marjan Zarif-Yeganeh Parichehreh Yaghmaei Mohammad Hossein Modarressi Mehdi Hedayati Source Type: research

New evidence on tumor suppressor activity of PTEN and KLLN in papillary thyroid carcinoma
This study aimed to address the hypothesis that the expression of PTEN and KLLN tumor suppressor genes could diminish in papillary thyroid cancer (PTC) compared to paired normal tissue (PNT) and multinodular goiter (MNG). PTEN and KLLN expressions were assessed at both mRNA and protein levels in 82 tissue samples, including 30 PTC, 30 PNT, and 26 MNG using SYBR-Green Real-Time PCR and enzyme-linked immunosorbent assay (ELISA), respectively. Bioinformatics studies were performed to evaluate the genomic location and the genes promoter region. The mRNA expression of PTEN and KLLN in PTC was significantly lower than PNT (PTEN,...
Source: Pathology, Research and Practice - August 23, 2021 Category: Pathology Authors: S Adeleh Razavi Pouya Salehipour Hanieh Gholami Sara Sheikholeslami Marjan Zarif-Yeganeh Parichehreh Yaghmaei Mohammad Hossein Modarressi Mehdi Hedayati Source Type: research

New evidence on tumor suppressor activity of PTEN and KLLN in papillary thyroid carcinoma
This study aimed to address the hypothesis that the expression of PTEN and KLLN tumor suppressor genes could diminish in papillary thyroid cancer (PTC) compared to paired normal tissue (PNT) and multinodular goiter (MNG). PTEN and KLLN expressions were assessed at both mRNA and protein levels in 82 tissue samples, including 30 PTC, 30 PNT, and 26 MNG using SYBR-Green Real-Time PCR and enzyme-linked immunosorbent assay (ELISA), respectively. Bioinformatics studies were performed to evaluate the genomic location and the genes promoter region. The mRNA expression of PTEN and KLLN in PTC was significantly lower than PNT (PTEN,...
Source: Pathology, Research and Practice - August 23, 2021 Category: Pathology Authors: S Adeleh Razavi Pouya Salehipour Hanieh Gholami Sara Sheikholeslami Marjan Zarif-Yeganeh Parichehreh Yaghmaei Mohammad Hossein Modarressi Mehdi Hedayati Source Type: research

New evidence on tumor suppressor activity of PTEN and KLLN in papillary thyroid carcinoma
This study aimed to address the hypothesis that the expression of PTEN and KLLN tumor suppressor genes could diminish in papillary thyroid cancer (PTC) compared to paired normal tissue (PNT) and multinodular goiter (MNG). PTEN and KLLN expressions were assessed at both mRNA and protein levels in 82 tissue samples, including 30 PTC, 30 PNT, and 26 MNG using SYBR-Green Real-Time PCR and enzyme-linked immunosorbent assay (ELISA), respectively. Bioinformatics studies were performed to evaluate the genomic location and the genes promoter region. The mRNA expression of PTEN and KLLN in PTC was significantly lower than PNT (PTEN,...
Source: Pathology, Research and Practice - August 23, 2021 Category: Pathology Authors: S Adeleh Razavi Pouya Salehipour Hanieh Gholami Sara Sheikholeslami Marjan Zarif-Yeganeh Parichehreh Yaghmaei Mohammad Hossein Modarressi Mehdi Hedayati Source Type: research

New evidence on tumor suppressor activity of PTEN and KLLN in papillary thyroid carcinoma
This study aimed to address the hypothesis that the expression of PTEN and KLLN tumor suppressor genes could diminish in papillary thyroid cancer (PTC) compared to paired normal tissue (PNT) and multinodular goiter (MNG). PTEN and KLLN expressions were assessed at both mRNA and protein levels in 82 tissue samples, including 30 PTC, 30 PNT, and 26 MNG using SYBR-Green Real-Time PCR and enzyme-linked immunosorbent assay (ELISA), respectively. Bioinformatics studies were performed to evaluate the genomic location and the genes promoter region. The mRNA expression of PTEN and KLLN in PTC was significantly lower than PNT (PTEN,...
Source: Pathology, Research and Practice - August 23, 2021 Category: Pathology Authors: S Adeleh Razavi Pouya Salehipour Hanieh Gholami Sara Sheikholeslami Marjan Zarif-Yeganeh Parichehreh Yaghmaei Mohammad Hossein Modarressi Mehdi Hedayati Source Type: research

MicroRNAs in organ fibrosis: From molecular mechanisms to potential therapeutic targets
Pathol Res Pract. 2021 Aug 13;225:153588. doi: 10.1016/j.prp.2021.153588. Online ahead of print.ABSTRACTFibrosis is caused by chronic tissue injury and characterized by the excessive deposition of extracellular matrix (ECM) that ultimately results in organ failure and death. Owing to lacking of effective treatment against tissue fibrosis, it causes a high morbidity and mortality worldwide. Thus, it is of great importance to find an effective therapy strategy for the treatment of fibrosis. MicroRNAs (miRNAs) play vital roles in many biological processes by targeting downstream genes. Numerous studies demonstrated that miRNA...
Source: Pathology, Research and Practice - August 22, 2021 Category: Pathology Authors: Hui Zhao Ya-Long Feng Tian Liu Jing-Jing Wang Jun Yu Source Type: research

A novel 13 RNA binding proteins (RBPs) signature could predict prostate cancer biochemical recurrence
Pathol Res Pract. 2021 Aug 13;225:153587. doi: 10.1016/j.prp.2021.153587. Online ahead of print.ABSTRACTBACKGROUND: Cancer precision medicine requires biomarkers or signatures to predict prognosis and therapeutic benefits. Driven by this, we established a biochemical recurrence (BCR) predictive model for prostate cancer (PCA) patients based on RNA-binding proteins (RBPs).METHODS: RNA-sequencing and corresponding clinicopathological data were downloaded from the Cancer Genome Atlas (TCGA) database and the Gene Expression Omnibus (GEO) database. Univariate COX, LASSO and multivariate COX regression analyses were carried out ...
Source: Pathology, Research and Practice - August 22, 2021 Category: Pathology Authors: Qianwei Xing Shouyong Liu Jiaochen Luan Yi Wang Limin Ma Source Type: research

MicroRNAs in organ fibrosis: From molecular mechanisms to potential therapeutic targets
Pathol Res Pract. 2021 Aug 13;225:153588. doi: 10.1016/j.prp.2021.153588. Online ahead of print.ABSTRACTFibrosis is caused by chronic tissue injury and characterized by the excessive deposition of extracellular matrix (ECM) that ultimately results in organ failure and death. Owing to lacking of effective treatment against tissue fibrosis, it causes a high morbidity and mortality worldwide. Thus, it is of great importance to find an effective therapy strategy for the treatment of fibrosis. MicroRNAs (miRNAs) play vital roles in many biological processes by targeting downstream genes. Numerous studies demonstrated that miRNA...
Source: Pathology, Research and Practice - August 22, 2021 Category: Pathology Authors: Hui Zhao Ya-Long Feng Tian Liu Jing-Jing Wang Jun Yu Source Type: research