DAAM1-mediated migration and invasion of ovarian cancer cells are suppressed by miR-208a-5p.
Abstract Ovarian cancer (OvCa) has the highest morbidity among all gynecologic cancers worldwide, and its distant metastasis is one of main causes for the poor prognosis of OvCa patients. Our previous studies have reported that DAAM1-involved signaling pathways play vital roles in metastasis of breast cancer. However, whether DAAM1 participates in OvCa migration and/or invasion is still unknown. The impact of DAAM1 on cell migration and invasion in OvCa was evaluated by wound healing assay and Boyden chamber assay. The specific miRNA targeting DAAM1 was predicted by bioinformatics methods and verified by dual-luci...
Source: Pathology, Research and Practice - May 14, 2019 Category: Pathology Authors: Mei J, Huang Y, Hao L, Liu Y, Yan T, Qiu T, Xu R, Xu B, Xiao Z, Jiang X, Hu K, Zhu Y Tags: Pathol Res Pract Source Type: research

Our approach to bone marrow biopsies in cytopenia.
Abstract Unexplained cytopenia is one of the most common indications for performing trephine bone marrow (BM) biopsy (BMB). The histopathological examination in this regard must be seen in the broader context of a multimodal approach in order to reach an as entity-specific as possible diagnosis, considering medical history, physical examination, laboratory data, peripheral blood morphology, BM aspiration smear, flow cytometry results and, if indicated, cytogenetics and molecular genetics. The particular irreplaceability of the histopathological work-up and the expectations to the BMB lie especially in the detectio...
Source: Pathology, Research and Practice - May 9, 2019 Category: Pathology Authors: Le Clef Q, Menter T, Tzankov A Tags: Pathol Res Pract Source Type: research

Significance of SATB2 expression in colon cancer and its differential diagnosis in digestive tract adenocarcinoma and ovarian primary and metastatic carcinoma.
Abstract OBJECTIVE: To study the relationship between the expression of SATB2 in colonic adenocarcinoma and clinicopathological factors, and its role in the identification of ovarian digestive system metastatic adenocarcinoma. METHODS: Immunohistochemistry was used to analyze the expression of SATB2 in 130 cases of colon adenocarcinoma, 46 cases of gastric adenocarcinoma, 47 cases of pancreatic ductal adenocarcinoma, 22 cases of cholangiocarcinoma, 53 cases of ovarian mucinous adenocarcinoma, and 38 cases of ovarian metastasis of colorectal carcinoma. Data was analyzed using SPSS 16.0 statistical software. ...
Source: Pathology, Research and Practice - May 7, 2019 Category: Pathology Authors: Liu F, Gao Z, Shen D, Zhao H, Wang C, Ye Y, Ma Y, Kong F Tags: Pathol Res Pract Source Type: research

Histopathologic characteristics of advanced-stage ROS1-rearranged non-small cell lung cancers.
CONCLUSIONS: Our findings highlight distinct histologic features of ROS1-rearranged tumors, including their nuclear features. A thorough understanding of ROS1 rearrangement-related histologic features would be helpful to identify ROS1-rearranged tumors in advanced-stage NSCLC. PMID: 31085007 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 6, 2019 Category: Pathology Authors: Park E, Choi YL, Ahn MJ, Han J Tags: Pathol Res Pract Source Type: research

Association between GPX3 promoter methylation and malignant tumors: A meta-analysis.
Abstract Glutathione peroxidase 3 (GPX3) has an important function of scavenging hydrogen peroxide and preventing cancer. The purpose of this meta-analysis was to analyze the relationship between GPX3 gene methylation and cancer and to further evaluate its diagnostic value for cancer. We screened eligible literatures from the PubMed, Embase, CNKI and Wanfang databases. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were used to measure the association of GPX3 methylation with cancer. Summary receiver operating characteristics (SROC) analysis was used to assess the diagnostic value of GPX3 methylation for...
Source: Pathology, Research and Practice - May 6, 2019 Category: Pathology Authors: Zhou C, Hu H, Zheng Z, Chen C, Li Y, Li B, Duan S Tags: Pathol Res Pract Source Type: research

Prognostic significance of IFITM1 expression and correlation with microvessel density and epithelial-mesenchymal transition signature in lung adenocarcinoma.
Abstract We evaluated the relationship between interferon-induced transmembrane protein 1 (IFITM1) expression, epithelial-mesenchymal transition (EMT) signature and angiogenesis in lung adenocarcinoma. Additionally, we examined prognostic significance of IFITM1 according to pTNM stage to confirm that IFITM1 can serve as a complement to the pTNM stage. A total of 141 lung adenocarcinoma specimens were evaluated retrospectively by immunohistochemical staining for IFITM1, EMT markers (e-cadherin, β-catenin, and vimentin), and CD31 to measure microvessel density. IFITM1was expressed in 46.8% of the specimens. IFI...
Source: Pathology, Research and Practice - May 6, 2019 Category: Pathology Authors: Koh YW, Han JH, Jeong D, Kim CJ Tags: Pathol Res Pract Source Type: research

SOX11 promotes osteoarthritis through induction of TNF- α.
CONCLUSION: Inhibition of SOX11 could improve IL-1β-induced OA like phenomenon in CHON-001 cells, which suggesting SOX11 played an important role during the pathogenesis of OA. Thus, we hypothesized that SOX11 could be a potential target for the treatment of patients with OA. PMID: 31078342 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 6, 2019 Category: Pathology Authors: Xu S, Yu J, Wang Z, Ni C, Xia L, Tang T Tags: Pathol Res Pract Source Type: research

Upregulation of MIIP regulates human breast cancer proliferation, invasion and migration by mediated by IGFBP2.
CONCLUSION: The abnormal expression of MIIP in breast cancer affects the cell biological effects. IGFBP2 was regulated via MIIP which may be associated with these biological effects. These results reveal that MIIP can be a potential target for breast cancer treatment. PMID: 31078343 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 6, 2019 Category: Pathology Authors: Du Y, Wang P Tags: Pathol Res Pract Source Type: research

Immunohistochemical profiling of mucins in sinonasal adenocarcinomas.
In this study we investigated the expression of mucins (MUC1, MUC2, MUC4, MUC5AC and MUC6) in a series of 66 sinonasal adenocarcinomas, in order to establish their distribution and the possible correlation with clinicopathological and prognostic parameters. The series included 51 intestinal type adenocarcinomas, 4 non-intestinal type adenocarcinomas, and 11 salivary gland type carcinomas. The immunohistochemical analysis was conducted on a tissue microarray obtained from formalin fixed-paraffin embedded tumor tissue samples. Thirty-nine adenocarcinomas (59.1%) resulted positive for MUC1, 21 (41.2%) for MUC2, 47 (71.2%) for...
Source: Pathology, Research and Practice - May 4, 2019 Category: Pathology Authors: Taverna C, Maggiore G, Cannavicci A, Bonomo P, Santucci M, Franchi A Tags: Pathol Res Pract Source Type: research

Identification of core genes and potential molecular mechanisms in breast cancer using bioinformatics analysis.
CONCLUSION: These results identified key genes, which could be used as a new biomarker for breast cancer diagnosis and treatment. PMID: 31076281 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 4, 2019 Category: Pathology Authors: Liu F, Wu Y, Mi Y, Gu L, Sang M, Geng C Tags: Pathol Res Pract Source Type: research

Identification of cancer-related gene network in hepatocellular carcinoma by combined bioinformatic approach and experimental validation.
Abstract HCC (hepatocellular carcinoma) is a highly aggressive malignancy that cause a mass of deaths world widely. We chose gene expression datasets of GSE27635 and GSE28248 from GEO database to find out key genes and their interaction network during the progression and metastasis of HCC. GEO2R online tool was used to screen differentially expressed genes (DEGs) between tumor and peri-tumor tissues based on these two datasets. The identified differentially expressed genes were prepared for further analysis such as GO function, KEGG pathway, PPI network analysis using Database for Annotation, Visualization and Int...
Source: Pathology, Research and Practice - May 4, 2019 Category: Pathology Authors: Ni W, Zhang S, Jiang B, Ni R, Xiao M, Lu C, Liu J, Qu L, Ni H, Zhang W, Zhou P Tags: Pathol Res Pract Source Type: research

Long noncoding RNAs in bladder cancer prognosis: A meta-analysis.
Abstract BACKGROUND: Numerous studies have demonstrated the involvement of long non-coding RNAs (lncRNAs) in the tumorigenesis of bladder cancer (BC). The aim of this study was to investigate the possible correlations between the specific lncRNAs and the clinical outcomes in bladder cancer patients. METHODS: We systematically searched the PubMed, EMBASE and the Cochrane Library databases for studies published up to October 15, 2018, and retrieved the suitable articles. Pooled odds ratios (ORs), hazard ratios (HRs) and 95% confidence intervals (95% CIs) were obtained by using fixed-effect or random-effect mode...
Source: Pathology, Research and Practice - April 29, 2019 Category: Pathology Authors: Zhu W, Liu H, Wang X, Lu J, Yang W Tags: Pathol Res Pract Source Type: research

Decreased expression of lncRNA loc285194 as an independent prognostic marker in cancer: A systematic review and meta-analysis.
Abstract BACKGROUND: Several studies have indicated that lncRNA loc285194 is aberrantly expressed in many types of cancer. This meta-analysis was performed to elucidate the potential role of lncRNA loc285194 as a prognostic marker in malignant tumors. METHODS: An electronic search of PubMed, Medline, Embase, and Web of Science was performed to identify all eligible papers related to the prognostic impact of lncRNA loc285194 expression in cancer. Hazard ratios (HR) and 95% confidence intervals (CI) were extracted from the included studies to explore the association between lncRNA loc285194 expression and patie...
Source: Pathology, Research and Practice - April 28, 2019 Category: Pathology Authors: Mehrad-Majd H, Ravanshad S, Moradi A, Khansalar N, Sheikhi M, Akhtari J Tags: Pathol Res Pract Source Type: research

HER2 status in sinonasal intestinal-type adenocarcinoma.
Abstract Given that the prognosis of patients with sinonasal intestinal-type adenocarcinoma (ITAC) has not significantly changed recently, there is a desire for new therapeutic approaches to improve clinical management. HER2-targeted therapy has remarkably improved the overall survival of patients with HER2 amplified tumors. To date, HER2 assessment has produced contradictory results in ITAC. The aim of this study was to assess HER2 status at both protein and DNA levels in a large series of ITAC. HER2 status was assessed by immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH) in forty-three pati...
Source: Pathology, Research and Practice - April 28, 2019 Category: Pathology Authors: Maffeis V, Cappellesso R, Zanon A, Cazzador D, Emanuelli E, Martini A, Fassina A Tags: Pathol Res Pract Source Type: research

Prognostic role of Amphiregulin and the correlation with androgen receptor in invasive breast cancer.
Abstract BACKGROUND: In androgen-sensitive prostate cancer, androgenic stimulation induces the synthesis of amphiregulin (AREG). Research is lacking on the role of AREG in invasive breast cancer and the co-expression with androgen receptor (AR) status. MATERIALS AND METHODS: The present study investigated the prognostic role of AREG in invasive breast cancer cases (N = 298) and the co-expression with the AR status as analysed by immunohistochemistry (IHC). RESULTS: The samples were divided into groups according to AREG expression levels: low/no expression (AREGlow/no) and high expression (A...
Source: Pathology, Research and Practice - April 22, 2019 Category: Pathology Authors: Xiang G, Liu F, Liu J, Meng Q, Li N, Niu Y Tags: Pathol Res Pract Source Type: research

Expression of programmed cell death-ligand 1 in oral squamous cell carcinoma and oral leukoplakia is associated with disease progress and CD8+ tumor-infiltrating lymphocytes.
Abstract OBJECTIVE: In recent years, monoclonal antibodies targeting programmed cell death-ligand 1 (PD-L1) have become a promising cancer immunotherapy. However, the role of PD-L1 in oral squamous cell carcinoma (OSCC) and oral potentially malignant disorders (OPMDs), including oral leukoplakia (OLK), remains controversial. The aim of the present study was to investigate the expression level of PD-L1 in OSCC and OPMDs, and examine its relationship with CD8 expression and different clinicopathological features. METHOD: Expression of PD-L1 and CD8 were conducted in 41 OSCC, 21 OLK, and 25 normal mucosa samples...
Source: Pathology, Research and Practice - April 18, 2019 Category: Pathology Authors: Chen XJ, Tan YQ, Zhang N, He MJ, Zhou G Tags: Pathol Res Pract Source Type: research

Pyruvate kinase M2 contributes to cell growth in gastric cancer via aerobic glycolysis.
In conclusion, the results of this study indicated that inhibition of PKM2 could represent a novel strategy for gastric cancer treatment. PMID: 31000383 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 7, 2019 Category: Pathology Authors: Li H, Xu H, Xing R, Pan Y, Li W, Cui J, Lu Y Tags: Pathol Res Pract Source Type: research

Overexpression of FER1L4 promotes the apoptosis and suppresses epithelial-mesenchymal transition and stemness markers via activating PI3K/AKT signaling pathway in osteosarcoma cells.
In conclusion, the study indicated that FER1L4 acted as a tumor suppressor in osteosarcoma via activating PI3K/AKT pathway may be a new prognostic biomarker and potential therapeutic target for osteosarcoma intervention. PMID: 31000382 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 6, 2019 Category: Pathology Authors: Ma L, Zhang L, Guo A, Liu LC, Yu F, Diao N, Xu C, Wang D Tags: Pathol Res Pract Source Type: research

Identification of circulating miR-663a as a potential biomarker for diagnosing osteosarcoma.
CONCLUSION: Plasma miR-663a was identified as a novel potential biomarker for the diagnosis of OS. PMID: 30987831 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 6, 2019 Category: Pathology Authors: Huang C, Sun Y, Ma S, Vadamootoo AS, Wang L, Jin C Tags: Pathol Res Pract Source Type: research

Diagnostic and prognostic roles of CK20 in the pathology of urothelial lesions. A systematic review.
mio L Abstract Cytokeratin 20 (CK20) is one of the most common immunohistochemical markers in the routine practice of a pathology lab, as biopsies from the urinary tract encompass a wide spectrum of lesions which may pose issues in their detection and classification. In this review, we aim to outline the diagnostic accuracy and prognostic value of CK20 in flat urothelial lesions, papillary non-invasive and invasive urothelial carcinoma, molecular subgroups and variant histology, and we briefly discuss its limitations and potential pitfalls. PMID: 30987832 [PubMed - as supplied by publisher] (Source: Pathology...
Source: Pathology, Research and Practice - April 6, 2019 Category: Pathology Authors: Sanguedolce F, Russo D, Calò B, Cindolo L, Carrieri G, Cormio L Tags: Pathol Res Pract Source Type: research

The metalloprotease ADAM17 in inflammation and cancer.
rbers C Abstract Proteolytic cleavage of transmembrane proteins is an important post-translational modification that regulates the biological function of numerous transmembrane proteins. Among the 560 proteases encoded in the human genome, the metalloprotease A Disintegrin and Metalloprotease 17 (ADAM17) has gained much attention in recent years and has emerged as a central regulatory hub in inflammation, immunity and cancer development. In order to do so, ADAM17 cleaves a variety of substrates, among them the interleukin-6 receptor (IL-6R), the pro-inflammatory cytokine tumor necrosis factor α (TNFα) ...
Source: Pathology, Research and Practice - April 6, 2019 Category: Pathology Authors: Düsterhöft S, Lokau J, Garbers C Tags: Pathol Res Pract Source Type: research

Integrative immunologic and genomic characterization of brain metastasis from ovarian/peritoneal cancer.
We report immunologic and genomic profiles of primary ovarian/peritoneal cancer with brain metastasis that may not only provide useful information for understanding its pathogenesis, but also clues for further innovative therapeutic treatments for ovarian cancer. PMID: 30962002 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 3, 2019 Category: Pathology Authors: Choi YJ, Kim SY, Park HC, Chung YJ, Hur SY, Lee SH Tags: Pathol Res Pract Source Type: research

Decreased expression levels of DAL-1 and TOB1 are associated with clinicopathological features and poor prognosis in gastric cancer.
Abstract PURPOSE: We previously demonstrated that the functional inactivation of DAL-1 and TOB1 promotes an aggressive phenotype in gastric cancer cells, but the links between both genes and the survival of patients with gastric cancer are unknown. Here, we investigated the correlations of the expression levels of DAL-1 and TOB1 with the progression of gastric cancer. METHODS: A total of 270 patients who underwent resectable gastrectomy were included. The expression of DAL-1 and TOB1 was detected by immunohistochemistry. RESULTS: Low expression of DAL-1 in cancer tissue was significantly associated with ...
Source: Pathology, Research and Practice - April 2, 2019 Category: Pathology Authors: Guo H, Zhang R, Afrifa J, Wang Y, Yu J Tags: Pathol Res Pract Source Type: research

Overexpression of Alteration/Deficiency in Activation 3 correlates with poor prognosis in non-small cell lung cancer.
Abstract Alteration/Deficiency in Activation 3 (ADA3), the human homologue of yeast ADA3, is involved in a variety of cell biological processes and plays an important role in tumorigenesis. Here, western blotting and reverse-transcription quantitative PCR (RT-qPCR) were conducted to explore the expression pattern of ADA3 in non-small cell lung cancer (NSCLC) patients. It was found that ADA3 protein expression in cancerous tissues was significantly higher than that in adjacent normal lung tissues, but there were no differences in mRNA levels. Tissue microarray immunohistochemical assay (TMA-IHC) was performed and w...
Source: Pathology, Research and Practice - April 2, 2019 Category: Pathology Authors: Zhang S, Xu L, Tang Z, Wang H, Gu J, Sun B, Zhang W, Niu D, Feng J Tags: Pathol Res Pract Source Type: research

Tissue expansion of lung bronchi due to tissue processing for histology - A comparative analysis of paraffin versus frozen sections in a pig model.
Abstract AIM: Tissue shrinking due to fixation and processing is well known. However, the degree of shrinking varies significantly with the tissue type as well as the processing method and is not well studied in various tissues. In daily pathological routine workflow, histological specimens from frozen and paraffin sections are performed from the same tissue. In the present study we compared the thickness of bronchus walls obtained from paraffin and frozen sections. METHODS: Pig lungs were frozen in ventilated condition in liquid nitrogen and 36 bronchi were isolated after dissection. Frozen sections of 5&thi...
Source: Pathology, Research and Practice - March 30, 2019 Category: Pathology Authors: Schmitt VH, Schmitt C, Hollemann D, Weinheimer O, Mamilos A, Kirkpatrick CJ, Brochhausen C Tags: Pathol Res Pract Source Type: research

Spindle cell rhabdomyosarcoma in a lumbar vertebra with FUS-TFCP2 fusion.
Abstract A 70-year-old woman developed severe buttock pain that progressed to a walking disturbance. Radiographs and computed tomography scans revealed an osteolytic lesion with osteosclerosis extending from the body to the arch of the fifth lumbar vertebra. Magnetic resonance imaging showed multinodular masses in the fifth lumbar vertebral body extending into the spinous processes and right transverse process. The masses were hypointense to isointense on T1-weighted images and hypointense to hyperintense on T2-weighted images. Histologic examination of biopsy specimens showed destruction of the trabecula of the v...
Source: Pathology, Research and Practice - March 27, 2019 Category: Pathology Authors: Tagami Y, Sugita S, Kubo T, Iesato N, Emori M, Takada K, Tsujiwaki M, Segawa K, Sugawara T, Kikuchi T, Hasegawa T Tags: Pathol Res Pract Source Type: research

Subcutaneous panniculitis-like T-cell lymphoma in a 25-year-old male patient with sickle cell disease.
Abstract Sickle cell disease (SCD) is a hereditary blood disorder that often has multiple comorbidities. Patients occasionally develop malignant neoplasms, but the risk of lymphoma in SCD is currently unknown. Here, we report a unique case of subcutaneous panniculitis-like T-cell lymphoma (SPTCL) in a 25-year-old male patient with SCD. The patient suffered from episodes of sickling crisis since his initial SCD diagnosis and had been treated with supportive care. Hydroxyurea was added at the age of 23 years old. Two years later, he presented with right cheek swelling, and the biopsy showed a lymphohistiocytic infil...
Source: Pathology, Research and Practice - March 27, 2019 Category: Pathology Authors: Ma S, Zhao Y, Leudke C, Li Y, Sebastian S, Yang LH, Wang E Tags: Pathol Res Pract Source Type: research

MicroRNA-133b regulates the growth and migration of vascular smooth muscle cells by targeting matrix metallopeptidase 9.
Abstract Atherosclerosis is a systemic disease affecting the whole arterial tree of the human body, and it is the leading cause of cardiovascular diseases.Vascular smooth muscle cells (VSMCs) have been identified to play a key role in the development of atherosclerosis. MicroRNAs (miRNAs) are a group of endogenous small non-coding RNAs, and they play a critical role in many biological processes including regulating cell proliferation, migration and apoptosis. However, till now, the expression and role of miR-133b in atherosclerosis remain largely unknown. Therefore, our purpose was to investigate the expression an...
Source: Pathology, Research and Practice - March 20, 2019 Category: Pathology Authors: Liu H, Xiong W, Liu F, Lin F, He J, Liu C, Lin Y, Dong S Tags: Pathol Res Pract Source Type: research

IL-6 promotes endometrial cancer cells invasion and migration through signal transducers and activators of transcription 3 signaling pathway.
In this study, we aimed to investigate the role of IL-6/Stat3 signaling pathway in human endometrial cancer cells invasion and migration. We demonstrated that Stat3 is activated in endometrial cancer cell lines. To investigate the role of Stat3 in endometrial cancer invasive capacity, we used Stat3 inhibitor Stattic and found that Stattic significantly inhibited the migration and invasion of endometrial cancer cells elevated by IL-6. Furthermore, we showed that Stat3 inhibitor significantly decreased the expression of MMP2 enhanced by IL-6, indicating that IL-6 promoted endometrial cancer invasion and migration by Stat3-in...
Source: Pathology, Research and Practice - March 19, 2019 Category: Pathology Authors: Che Q, Xiao X, Liu M, Lu Y, Dong X, Liu S Tags: Pathol Res Pract Source Type: research

RNA N6-methyladenosine modification participates in miR-660/E2F3 axis-mediated inhibition of cell proliferation in gastric cancer.
Abstract Increasing evidence has shown that dysregulation of mircoRNA (miRNA) is linked to the development and progression of human cancer, including gastric cancer (GC). In the current study, by analysing the GEO database (GSE78091), we found that miR-660 was significantly downregulated in GC. Consistently, quantitative real-time PCR (qRT-PCR) results showed that miR-660 was dramatically decreased in GC tissues and cell lines. Importantly, low miR-660 expression was closely related to larger tumor size (P =  0.008), lymph node metastasis (P =  0.006), advanced TNM stage (P =&thi...
Source: Pathology, Research and Practice - March 19, 2019 Category: Pathology Authors: He X, Shu Y Tags: Pathol Res Pract Source Type: research

Inhibitory effects of bortezomib in a subcutaneous tumor model of H22 mouse hepatocarcinoma cells.
Abstract OBJECTIVE: To evaluate the inhibition effects and mechanism of bortezomib in a subcutaneous H22 mouse hepatocarcinoma model. METHODS: A subcutaneous xenograft model was constructed by subcutaneous injection of H22 cells in mice. The xenograft mice was randomly divided into bortezomib and control groups (n = 8 each). The bortezomib group was injected with 0.5 mg/kg bortezomib in saline via tail vein once every four days for a total of 4 times. The control group was intravenously given an equal volume of saline. The tumor size was measured every four days. At day 19, subcutaneous x...
Source: Pathology, Research and Practice - March 18, 2019 Category: Pathology Authors: Zhu M Tags: Pathol Res Pract Source Type: research

Clinical usefulness of gastric adenocarcinoma predictive long intergenic noncoding RNA in human malignancies: A meta-analysis.
This study was performed to systematically estimate the prognostic role of GAPLINC expression in cancer patients. METHODS: Several electronic databases, including PubMed, Embase, Web of Science, China National Knowledge Infrastructure (CNKI), and Wan Fang databases were searched for potential literature (updated to September 3, 2018). The pooled hazard ratios (HRs), odds ratios (ORs) and 95% confidence intervals (CIs) were calculated with a fixed effects model using Stata12.0 software. RESULTS: The pooled results indicated that elevated GAPLINC was significantly related to shorter overall survival (OS) (HR&thinsp...
Source: Pathology, Research and Practice - March 16, 2019 Category: Pathology Authors: Liu W, Xu J, Zhang C Tags: Pathol Res Pract Source Type: research

Aspirin inhibits hypoxia-mediated lung cancer cell stemness and exosome function.
CONCLUSIONS: Aspirin inhibits the hypoxia-induced stemness, hypoxic-mediated exosome release, and malignant paracrine effects of A549 cells. PMID: 30878308 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - March 11, 2019 Category: Pathology Authors: Chen J, Xu R, Xia J, Huang J, Su B, Wang S Tags: Pathol Res Pract Source Type: research

Cystic lymphangiomatous lesions of the adrenal gland: A clinicopathological study of 37 cases including previously unreported cysts with papillary endothelial proliferation.
In conclusion, adrenal lymphangiomatous cysts are usually asymptomatic, incidentally diagnosed lesions with a female predominance. They may imitate other adrenal tumors, both radiologically and clinically. Despite being non-functioning lesions, they should be considered as a possible cause of pseudopheochromocytoma. Although most adrenal lymphangiomatous cysts seem to be non-neoplastic, vascular abnormalities (malformations or lymphangiectasias), those with papillary endothelial proliferations may represent true neoplastic lesions. PMID: 30862368 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - March 6, 2019 Category: Pathology Authors: Koperski Ł, Pihowicz P, Anysz-Grodzicka A, Górnicka B Tags: Pathol Res Pract Source Type: research

"J üdisch versippt" and "materialistic": The marginalization of Walther E. Berblinger (1882-1966) in the Third Reich.
"Jüdisch versippt" and "materialistic": The marginalization of Walther E. Berblinger (1882-1966) in the Third Reich. Pathol Res Pract. 2019 Mar 01;: Authors: Sziranyi J, Kaiser S, Schmidt M, Gross D Abstract The pathologist Walther Berblinger (1882-1966) became famous for his scientific studies on internal secretion, namely on the pathology of the pituitary and the pineal gland. The results of his research on the hormonal control of the reproductive system contributed significantly to the consolidation of the young discipline of endocrinology. His later pioneering work on the ...
Source: Pathology, Research and Practice - March 1, 2019 Category: Pathology Authors: Sziranyi J, Kaiser S, Schmidt M, Gross D Tags: Pathol Res Pract Source Type: research

Suppression of puerarin on polymethylmethacrylate-induced lesion of peri-implant by inhibiting NF- κB activation in vitro and in vivo.
In this study, we evaluated the effects of PR on polymethylmethacrylate (PMMA)-induced lesion of peri-implant in vitro and in vivo, and explored its possible mechanism of action. Our results indicated that PR could inhibit PMMA-induced osteoclastogenesis in RAW264.7 cells with a dose-dependent manner in vitro and effectively down-regulate mRNA and protein expressions of matrix metalloprotein 9 (MMP-9), tumor necrosis factor (TNF)-α, interleukin (IL)-6, and receptor activator of nuclear factor (NF)-κB (RANK), primarily via the suppression of NF-κB signaling. Furthermore, we found that PMMA induction could ...
Source: Pathology, Research and Practice - March 1, 2019 Category: Pathology Authors: Yan B, Zhou H, Chu J, Cao X Tags: Pathol Res Pract Source Type: research

Intravascular (post-hysterectomy) leiomyoma (IVL) as late tumor thrombus within the inferior vena cava (IVC)-A rare case primarily imposing as IVC thrombus originating from left renal vein after former left nephrectomy status.
CONCLUSION: Intravascular leiomyoma is a benign tumor with invasive tendency, which can be considered a diagnostic and therapeutic challenge. It should be thoroughly investigated to be planned for a radical surgical removal. By possible adherence to the intraabdominal or -thoracic organs, an interdisciplinary and eventually step-wise surgical approach (combining vascular, abdominal, thoracic and heart surgery as well as gynecology and urology), which can be demanding, is recommended to be seriously considered to i) reliably achieve R0 resection status and, thus, ii) provide best outcome, quality of life and prognosis. ...
Source: Pathology, Research and Practice - February 28, 2019 Category: Pathology Authors: Ghanem M, Meyer F, Jechorek D, Schoeder V, Ignatov A, Fadel M, Halloul Z Tags: Pathol Res Pract Source Type: research

A practical strategy for immunofluorescent detecting multiple targets in mouse tissues without restrictions on the host specious resources of the primary antibodies.
Abstract Commercial deficiency of practical system to label multiple targets in experimental mouse tissues significantly hinders the feasibility to study the potential association between/among multiple targets using tissue-based immunofluorescence (IF) staining. We have developed a new protocol to do dual - labeling immunofluorescences on mouse tissues by combining direct and indirect immunofluorescence, making it possible to use commercial antibodies from the same specious (rabbit) to detect multiple targets in formalin-fixed paraffin-embedded (FFPE) archival mouse tissues simultaneously. This method applies ind...
Source: Pathology, Research and Practice - February 28, 2019 Category: Pathology Authors: Yu S, He X, Drelich A, Judy B, Chang Q, Cao S, Ksiazek T, Xu Z, Gong B Tags: Pathol Res Pract Source Type: research

The POLR2E rs3787016 polymorphism is strongly associated with the risk of female breast and cervical cancer.
Abstract The rs3787016 polymorphism, in polymerase II polypeptide E (POLR2E), was previously identified as being associated with the risk for prostate cancer, esophageal cancer, breast cancer, papillary thyroid carcinoma and liver cancer, suggesting that rs3787016 may server as a common genetic factor to affect individual susceptibility to cancer. To prove the hypothesis, we here performed a case-control study to explore the association between rs3787016 and cervical cancer risk, and to confirm the association between rs3787016 and breast cancer in a central Chinese population, which was followed by a meta-analysi...
Source: Pathology, Research and Practice - February 27, 2019 Category: Pathology Authors: Chen B, Jiao Y, Yaolong F, Li T, Liu Y, Wang M, Xiuli G, Feng X Tags: Pathol Res Pract Source Type: research

Factors affecting KRAS mutation detection in colorectal cancer tissue.
CONCLUSIONS: The biopsy specimens showed better sample DNA quality for KRAS mutation test compared to resection specimens. In a routine diagnostic pathology setting, the cold ischemia time was an important factor affecting DNA quality and the formalin fixation had a wide time range for optimal DNA quality. PMID: 30846412 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 27, 2019 Category: Pathology Authors: Yun HG, Lee HJ, Kim BR, Lee JH, Lee JH, Lee MY, Kim DH, Sohn JH, Chae SW, Do IG, Do SI, Kim K Tags: Pathol Res Pract Source Type: research

Increased expression of GEF-H1 promotes colon cancer progression by RhoA signaling.
This study attempted to elucidate how GEF-H1 drives tumor formation, motility, invasion and metastasis in colon cancer (CC). The expression of GEF-H1 in CC tissue microarrays (TMAs) was analyzed by immunohistochemistry (IHC). GEF-H1 was upregulated in CC tissues compared with adjacent non-tumoral tissues. In addition, we found that high GEF-H1 expression correlated with shorter overall survival and distant metastasis. Migration and invasion assays showed that GEF-H1 upregulation increased CC cell motility, invasion and metastasis. In contrast, functional knockdown of GEF-H1 by RNAi rescued the effects caused by GEF-H1 over...
Source: Pathology, Research and Practice - February 27, 2019 Category: Pathology Authors: Cao J, Yang T, Tang D, Zhou F, Qian Y, Zou X Tags: Pathol Res Pract Source Type: research

Knockdown of KDM1B inhibits cell proliferation and induces apoptosis of pancreatic cancer cells.
In this study, we attempted to evaluate the functional role of KDM1B in PC cells. The expression of KDM1B was detected by immunohistochemistry and immunoblotting in PC tissues and cells. Lentivirus-mediated shRNA was applied to silence KDM1B in PANC-1 and SW1990 cells. Cell proliferation was measured by MTT and Celigo assay. Cell apoptosis was determined by both Caspase-Glo®3/7 assay and Flow cytometry. Intracellular signaling molecules were detected using a PathScan intracellular signaling array kit. In this study, we found KDM1B was highly expressed in PC tissues compared to paracancerous tissues. Moreover, elevated ...
Source: Pathology, Research and Practice - February 27, 2019 Category: Pathology Authors: Wang Y, Sun L, Luo Y, He S Tags: Pathol Res Pract Source Type: research

Expression of vimentin in nasopharyngeal carcinoma and its possible molecular mechanism: A study based on immunohistochemistry and bioinformatics analysis.
CONCLUSION: The high expression of vimentin plays a pivotal role in the development and poor progression of NPC, which indicates that vimentin may be an effective predictive indicator for NPC. PMID: 30833029 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 27, 2019 Category: Pathology Authors: Lu W, Luo JY, Wu MH, Hou JY, Yang X, Chen G, Feng ZB Tags: Pathol Res Pract Source Type: research

CHPF promotes lung adenocarcinoma proliferation and anti-apoptosis via the MAPK pathway.
CONCLUSION: CHPF can promote the proliferation and antiapoptosis of LUAD cells, which is promising to become a potential target for LUAD treatment. PMID: 30826152 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 16, 2019 Category: Pathology Authors: Hou XM, Zhang T, Da Z, Wu XA Tags: Pathol Res Pract Source Type: research

Sinonasal glomangiopericytoma: A clinicopathologic study.
Abstract Sinonasal glomangiopericytoma (SNGP) is a neoplasm arising in the nasal cavity and paranasal sinuses that shows perivascular myoid differentiation. The diagnosis of SNGP may be diagnostically challenging due to a large number of potential mimics. In the present study, we sought to characterize the histological and molecular features of six cases of SNGP found in prior surgical pathology records over a 15-year period. The average age at diagnosis was 48.5 years (range: 31-78 years), and the male-to-female ratio was 1:1. Imaging studies in all six cases demonstrated avidly enhancing, lobulated soft tissue m...
Source: Pathology, Research and Practice - February 3, 2019 Category: Pathology Authors: Obeidin F, Jennings LJ, Alexiev BA Tags: Pathol Res Pract Source Type: research

Molecular characterization of "sessile serrated" adenoma to carcinoma transition in six early colorectal cancers.
This study provides the molecular evidence that SSA/P, even without cytological dysplasia, is a precursor lesion of CRC and that conventional CRC might arise from mixed polyp. PMID: 30738693 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 3, 2019 Category: Pathology Authors: Cappellesso R, Lo Mele M, Munari G, Rosa-Rizzotto E, Guido E, De Lazzari F, Pilati P, Tonello M, Farinati F, Realdon S, Fassan M, Rugge M Tags: Pathol Res Pract Source Type: research

Syndecan-1 could be added to hormonal receptors and HER2/neu in routine assessment of invasive breast carcinoma, relation of its expression to prognosis and clinicopathological parameters.
CONCLUSION: syndecan-1 is a good predictor of poor overall survival and recurrence/ metastasis free survival. It is associated with aggressive phenotype as HER2 enriched and Triple negative rather than luminal subtypes of breast carcinoma. So it can be added to the hormonal receptors and HER 2 assay in the routine management of invasive breast cancer after confirmation on a more larger study. PMID: 30738694 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 2, 2019 Category: Pathology Authors: Soliman NA, Yussif SM, Shebl AM Tags: Pathol Res Pract Source Type: research

Overexpression of TONSL might be an independent unfavorable prognostic indicator in hepatocellular carcinoma.
This study was aimed to identify the expression of TONSL and its role in hepatocellular carcinoma (HCC). METHODS: By data mining in the Cancer Genome Atlas (TCGA) and Human Protein Atlas (HPA) databases, the expression profile of TONSL, its clinical significance, the potential mechanisms of its dysregulation and its underlying biological function in HCC were investigated. RESULTS: TONSL was significantly upregulated in HCC tissues relative to normal liver tissues (P 
Source: Pathology, Research and Practice - January 30, 2019 Category: Pathology Authors: Yu B, Ding Y, Liao X, Wang C, Wang B, Chen X Tags: Pathol Res Pract Source Type: research

Impact of isolated tumor cells in regional lymph nodes in adeno-and squamous cell carcinoma of the esophagus and the esophagogastric junction-A systematic review.
CONCLUSION: Although, the results were conflicting, ITC appeared to be a negative prognostic factor in esophageal and EGJ cancer. However, heterogeneity between the studies did not allow for a definitive conclusion. PMID: 30723054 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - January 30, 2019 Category: Pathology Authors: Lütken CD, Fiehn AK, Federspiel B, Achiam MP Tags: Pathol Res Pract Source Type: research

Oncogenicity of lncRNA FOXD2-AS1 and its molecular mechanisms in human cancers.
CONCLUSIONS: LncRNA FOXD2-AS1 is an oncogene and probably represents a feasible biomarker or therapeutic target in human cancers. PMID: 30723052 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - January 29, 2019 Category: Pathology Authors: Hu Q, Tai S, Wang J Tags: Pathol Res Pract Source Type: research