Histological assessment & use of immunohistochemical markers for detection of dysplasia in Barrett's esophageal mucosa.
CONCLUSIONS: A detail histological scoring system may bring uniformity in histological interpretation of dysplasia in BE. Using a combined panel of IHC stains seems helpful in detection of dysplasia in BE, especially to differentiate the IFD and LGD changes in BE. PMID: 29764708 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 13, 2018 Category: Pathology Authors: Kinra P, Gahlot GPS, Yadav R, Baloda V, Makharia GK, Gupta SD, Das P Tags: Pathol Res Pract Source Type: research

Long-term prognostic impact of osteopontin and Dickkopf-related protein 1 in patients with hepatocellular carcinoma after hepatectomy.
CONCLUSIONS: Serum OPN and DKK1 levels of HCC patients could be considered as novel biomarkers showing prognostic significance after hepatectomy based on long-term survival data. PMID: 29753515 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 6, 2018 Category: Pathology Authors: Byeon H, Lee SD, Hong EK, Lee DE, Kim BH, Seo Y, Joo J, Han SS, Kim SH, Park SJ Tags: Pathol Res Pract Source Type: research

Comprehensive expression analysis of TNF-related apoptosis-inducing ligand and its receptors in colorectal cancer: Correlation with MAPK alterations and clinicopathological associations.
Abstract TNF-related, apoptosis-inducing ligand (TRAIL) apoptotic pathway constitutes a promising therapeutic target due to high selectivity and low toxicity of TRAIL targeting agents when administered in combination therapies. 106 colorectal cancers were examined for: relative mRNA expression of TRAIL pathway genes, decoy receptors TRAIL-R3 and TRAIL-R4 promoter methylation and the presence of KRAS, NRAS, BRAF mutations. Elevated mRNA levels were observed in 26%, 15%, 13%, 12% and 10% of the cases for TRAIL-R4, TRAIL-R3, TRAIL-R2, TRAIL-R1 and TRAIL genes respectively. Reduced mRNA levels were detected in 77%, 65...
Source: Pathology, Research and Practice - May 1, 2018 Category: Pathology Authors: Tsikalakis S, Chatziandreou I, Michalopoulos NV, Theodoropoulos GE, Sakellariou S, Korkolopoulou P, Patsouris E, Saetta AA Tags: Pathol Res Pract Source Type: research

MiR-21 up-regulation in ampullary adenocarcinoma and its pre-invasive lesions.
Abstract Poor information is available on the molecular landscape characterizing the carcinogenetic process leading to ampullary carcinoma. MiR-21 is one of the most frequently up-regulated miRNAs in pancreatic adenocarcinoma, a tumor sharing similar molecular features with ampullary adenocarcinomas (AVCs), above all with the pancreatic-biliary type. We profiled, by in situ hybridization (ISH), miR-21 expression in a series of 26 AVCs, 50 ampullary dysplastic lesions (35 low-grade [LG-IEN] and 15 high-grade [HG-IEN]) and 10 normal duodenal mucosa samples. The same series was investigated by immunohistochemistry fo...
Source: Pathology, Research and Practice - May 1, 2018 Category: Pathology Authors: Saraggi D, Galuppini F, Fanelli GN, Remo A, Urso EDL, Bao RQ, Bacchin D, Guzzardo V, Luchini C, Braconi C, Farinati F, Rugge M, Fassan M Tags: Pathol Res Pract Source Type: research

Proteins of the retinoblastoma pathway, FEN1 and MGMT are novel potential prognostic biomarkers in pancreatic adenocarcinoma.
CONCLUSIONS: Major cell-cycle regulator also have predictive significance, but further studies are required to evaluate this. PMID: 29735403 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 1, 2018 Category: Pathology Authors: Isohookana J, Haapasaari KM, Soini Y, Leppänen J, Karihtala P Tags: Pathol Res Pract Source Type: research

Prognostic value of EGFR family expression in lymph node-negative esophageal squamous cell carcinoma patients.
This study was designed to determine the prognostic value of EGFR family expression in a population of lymph node-negative ESCC patients treated with curative resection. EGFR family protein expression was examined by immunohistochemical analysis of tissue microarrays of 94 patients with lymph node-negative ESCC after radical esophagectomy with three-field lymphadenectomy. Survival differences were compared using Kaplan-Meier analysis. Cox regression analyses were performed to determine the prognostic factors for overall survival and disease-free survival (DFS). ErbB4 expression was found to be an independent prognostic fac...
Source: Pathology, Research and Practice - April 30, 2018 Category: Pathology Authors: Yu W, Yang X, Chu L, Zhao K, Chen H, Xiang J, Zhang Y, Li H, Zhao W, Sun M, Wei Q, Fu X, Xie C, Zhu Z Tags: Pathol Res Pract Source Type: research

Expression of neuroendocrine differentiation markers in lethal metastatic castration-resistant prostate cancer.
In this study, we studied expression of three commonly used markers of NED (chromogranin A, neuron specific enolase and synaptophysin) in 89 metastases from 31 men that died of castration-resistant prostate cancer and underwent rapid autopsy, and in 89 hormone-naïve primary tumors removed by radical prostatectomy. In addition, we examined NED association with androgen receptor, ERG and Ki-67 expression in metastatic tumor sites. Morphologically, 1 of 31 cases was classified as small cell carcinoma, and the remaining 30 were classified as usual prostate adenocarcinoma using a recently proposed classification of prostat...
Source: Pathology, Research and Practice - April 26, 2018 Category: Pathology Authors: Sainio M, Visakorpi T, Tolonen T, Ilvesaro J, Bova GS Tags: Pathol Res Pract Source Type: research

LncRNA-DANCR: A valuable cancer related long non-coding RNA for human cancers.
CONCLUSIONS: Long non-coding RNAs-DANCR likely serves as a useful disease biomarker or therapeutic cancer target. PMID: 29728310 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 24, 2018 Category: Pathology Authors: Thin KZ, Liu X, Feng X, Raveendran S, Tu JC Tags: Pathol Res Pract Source Type: research

Upregulation of LncRNA FEZF-AS1 is associated with advanced clinical stages and family history of cancer in patients with NSCLC.
Abstract Antisense RNA (AS) is a type of long non-coding RNAs that functions as a post-transcriptional regulatory element on regulating parental coding gene expression via directly binding to complementary mRNA sequences. We aimed to investigate the effect of the AS to FEZF1 gene on non-small cell lung cancer (NSCLC) development. The expression level of lncRNA FEZF-AS1 and FEZF1 was determined by the quantitative Real-time PCR in 160 cases of NSCLC tissues and their adjacent non-tumour tissues. We found that lncRNA FEZF-AS1 was significantly up-regulated in tumour tissues when compared to the adjacent non-cancerou...
Source: Pathology, Research and Practice - April 24, 2018 Category: Pathology Authors: Gong W, Cao Y, Wang Y, Yang L, Su W, Qiu F, Datta S, Rao B, Xian J, Lin M, Feng Y, Zhang X, Zhou Y, Gao X, Lu J Tags: Pathol Res Pract Source Type: research

Intravenous leiomyomatosis of the uterus: A clinicopathologic analysis of 13 cases with an emphasis on histogenesis.
Abstract The object of this study is to investigate the prognosis and potential histologic origin of uterine intravenous leiomyomatosis (IVL). We retrospectively analyzed the clinicopathologic and immunohistochemical features of 13 cases of IVL from a single institute. The patients underwent hysterectomy (3 with bilateral salpingo-oophorectomy, and 8 with excision of the broad ligaments and/or parametrium). They survived with no evidence of disease for 6-90 months. Intravascular worm-like plugs were macroscopically identified in the myometrium in 10 of 13 cases (76.9%). Six patients (46.1%) had extra-uterine invol...
Source: Pathology, Research and Practice - April 22, 2018 Category: Pathology Authors: Tang L, Lu B Tags: Pathol Res Pract Source Type: research

Evaluation of specific modified histones in lip carcinogenesis.
CONCLUSIONS: Histone modifications are related to initial actinic damage, but not to malignant transformation in the lip. PMID: 29699903 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 22, 2018 Category: Pathology Authors: de Freitas Filho SAJ, Servato JPS, de Sá RT, Siqueira CS, de Faria PR, Loyola AM, Cardoso SV Tags: Pathol Res Pract Source Type: research

Tumorigenic role of YAP in hepatocellular carcinogenesis is involved in SHP2 whose function is different in vitro and in vivo.
This study aimed to investigate the tumorigenic roles of YAP and SHP2 in hepatocellular carcinogenesis. Cell density associated subcellular distributions of YAP and SHP2 in normal human hepatocytes (THLE-2) and hepatocellular carcinoma (HCC) cells (SK-Hep1, SNU-182) were investigated by Western blotting and cell block immunohistochemistry. The effects of YAP knockdown on proliferation, migration and invasion were studied using YAP-specific siRNAs. The prognostic significance of YAP and SHP2 expressions was investigated immunohistochemically using a tissue microarray (TMA) from 50 HCC cases. High-cell density decreased the ...
Source: Pathology, Research and Practice - April 21, 2018 Category: Pathology Authors: Kim MK, Park JY, Kang YN Tags: Pathol Res Pract Source Type: research

ALDH1 and tumor infiltrating lymphocytes as predictors for neoadjuvant chemotherapy response in breast cancer.
Abstract Many studies have reported that Aldehyde dehydrogenase 1 (ALDH1) and tumor-infiltrating lymphocytes (TIL) are related to breast cancer prognosis. However, the clinical significance of ALDH1 and tumor-infiltrating immune cells in breast cancer has not been fully investigated in patients who received neoadjuvant chemotherapy (NAC). We studied the significance of the expression of ALDH1 and the population of TIL for predicting the prognosis and chemotherapeutic response of patients with breast cancer who had received NAC. Forty patients who underwent NAC were enrolled in this study. ALDH1 and TIL (T cells an...
Source: Pathology, Research and Practice - April 18, 2018 Category: Pathology Authors: Lee A, Won KY, Lim SJ, Cho SY, Han SA, Park S, Song JY Tags: Pathol Res Pract Source Type: research

The SMYD3 VNTR 3/3 polymorphism confers an increased risk and poor prognosis of hepatocellular carcinoma in a Chinese population.
CONCLUSION: This study indicates that drinking history, family history of HCC and SMYD3 VNTR 3/3, TNM staging, maximum tumor diameter, LNM might be risk factors for HCC, and SMYD3 VNTR 3/3 might contribute to a lower 2- and 3-year survival rate of patients with HCC. PMID: 29691085 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 17, 2018 Category: Pathology Authors: Li RD, Tang YH, Wang HL, Yang D, Sun LJ, Li W Tags: Pathol Res Pract Source Type: research

In silico analysis of the potential mechanism of telocinobufagin on breast cancer MCF-7 cells.
CONCLUSIONS: Telocinobufagin might regulate neuroactive ligand-receptor interaction pathway to exert its influences in breast cancer MCF-7 cells, and its molecular mechanism might share some similarities with fenspiride. This study only presented a comprehensive picture of the role of telocinobufagin in breast cancer MCF-7 cells using big data. However, more thorough and deeper researches are required to add to the validity of this study. PMID: 29656985 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 5, 2018 Category: Pathology Authors: Dang YW, Lin P, Liu LM, He RQ, Zhang LJ, Peng ZG, Li XJ, Chen G Tags: Pathol Res Pract Source Type: research

Clinical implications of TERT promoter mutation on IDH mutation and MGMT promoter methylation in diffuse gliomas.
In conclusion, the combination of TERTp with IDH or MGMT status may be a prognostic indicator depending on grades. PMID: 29650441 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 5, 2018 Category: Pathology Authors: Kim HS, Kwon MJ, Song JH, Kim ES, Kim HY, Min KW Tags: Pathol Res Pract Source Type: research

Investigation of miR-136-5p key target genes and pathways in lung squamous cell cancer based on TCGA database and bioinformatics analysis.
CONCLUSIONS: Downregulated miR-136-5p may target UGT1A7 and ADH7 and participate in ascorbate and aldarate metabolism, pentose and glucuronate interconversions, and retinol metabolism. High expression of UGT1A7 and ADH7 may indicate better prognosis of LUSC patients. PMID: 29650443 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 5, 2018 Category: Pathology Authors: Xie ZC, Li TT, Gan BL, Gao X, Gao L, Chen G, Hu XH Tags: Pathol Res Pract Source Type: research

STR1720, a potential sensitizer for radiotherapy and cytotoxicity effects of NVB-BEZ235 in metastatic breast cancer cells.
Abstract BACKGROUND: Chemo-radio therapy (CRT) resistance is a main barrier in treating the triple negative breast cancer (TNBC). The success of conventional treatment may be ameliorated by elevating the responsiveness of the cancer cells to CRT. NVP-BEZ235 as a PI3K/AKT/mTOR dual inhibitor has been shown promising results in treating breast cancer cells. However, potential radiation-sensitizing effect of NVP-BEZ235 in TNBC remained unclear. In addition, SIRT-1 activation state and environmental cytokine were identified as being responsible for cancer cells responses to CRT. Herein, we investigate the role of inte...
Source: Pathology, Research and Practice - April 5, 2018 Category: Pathology Authors: Fatehi D, Soltani A, Ghatrehsamani M Tags: Pathol Res Pract Source Type: research

Histopathology of locally advanced colorectal carcinoma, with emphasis on tumor invasion of adherent peritoneal membranes.
Abstract Locally advanced colorectal carcinomas are characterized by neoplastic cells that invade beyond the colon and directly into surrounding organs and structures that include the retroperitoneum and abdominopelvic sidewall. These aggressive tumors are prognostically adverse and are categorized with highest possible tumor stage in current cancer staging systems. Recognizing colorectal carcinoma with extensive locoregional invasion is typically straightforward, but some cases can be diagnostically challenging. These include tumors with limited invasion of extracolonic tissue such as the subserosa in which there...
Source: Pathology, Research and Practice - April 3, 2018 Category: Pathology Authors: Chen JH Tags: Pathol Res Pract Source Type: research

Lower expression of bone marrow miR-122 is an independent risk factor for overall survival in cytogenetically normal acute myeloid leukemia.
CONCLUSIONS: Our findings indicated that lower expression of BM miR-122 acted as an independent risk factor for OS in CN-AML. PMID: 29627222 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 3, 2018 Category: Pathology Authors: Zhang TJ, Qian Z, Wen XM, Zhou JD, Li XX, Xu ZJ, Ma JC, Zhang ZH, Lin J, Qian J Tags: Pathol Res Pract Source Type: research

Use of the Ion AmpliSeq Cancer Hotspot Panel in clinical molecular pathology laboratories for analysis of solid tumours: With emphasis on validation with relevant single molecular pathology tests and the Oncomine Focus Assay.
Abstract Targeted application of next-generation sequencing (NGS) technology allows detection of specific mutations that can provide treatment opportunities for cancer patients. We evaluated the applicability of the Ion AmpliSeq Cancer Hotspot Panel V2 (CHV2) using formalin-fixed, paraffin-embedded (FFPE) tissue of clinical specimens. Thirty-five FFPE tumour samples with known mutational status were collected from four different hospitals and sequenced with CHV2 using an Ion Chef System and Ion S5 XL system. Out of 35 cases, seven were sequenced with Oncomine focus Assay Panel for comparison. For the limit of dete...
Source: Pathology, Research and Practice - March 29, 2018 Category: Pathology Authors: Lee A, Lee SH, Jung CK, Park G, Lee KY, Choi HJ, Min KO, Kim TJ, Lee EJ, Lee YS Tags: Pathol Res Pract Source Type: research

Immunohistochemical and molecular genetic study on epithelioid glioblastoma: Series of seven cases with review of literature.
Abstract Epithelioid glioblastoma (e-gbm) is a recently described variant of glioblastoma (GBM) which is associated with short survival and now added as a provisional entity to WHO 2016 classification of CNStumors. About half of these tumors show characteristic BRAF-V600E mutation. However, unlike conventional GBMs, e-gbm lack specific diagnostic and prognostic markers. Hence, we aimed to molecularly characterize these tumors. An extensive review of literature was performed.In a multi-institutional effort, all the cases of glioblastoma of year 2017 were reviewed. Cases with predominant epithelioid morphology were ...
Source: Pathology, Research and Practice - March 22, 2018 Category: Pathology Authors: Khanna G, Pathak P, Suri V, Sharma MC, Chaturvedi S, Ahuja A, Bhardwaj M, Garg A, Sarkar C, Sharma R Tags: Pathol Res Pract Source Type: research

Intrahepatic cholangiocarcinoma after Fontan procedure in an adult with visceral heterotaxy.
We report a unique case of intrahepatic cholangiocarcinoma 28 years after a Fontan procedure in a 31year old female with heterotaxy syndrome. The subcapsular mass-forming tumor was composed of poorly differentiated tumor cells arranged in small vague glandular or slit-lumen nests, and focally fused or anastomosing large trabecular patterns within the prominent fibrotic stroma. The tumor cells with immunoreactivity to CK7, CK19, Cam5.2, COX2, EMA, BCL-2, MOC-31 and AE1/AE3, supported a diagnosis of intrahepatic cholangiocarcinoma. Focal atypical ductular proliferation within the background liver may represent a precursor le...
Source: Pathology, Research and Practice - March 15, 2018 Category: Pathology Authors: Wang D, Marshall D, Veldtman G, Gupta A, Trout AT, Villafane J, Bove K Tags: Pathol Res Pract Source Type: research

COX-2 as a determinant of lower disease-free survival for patients affected by ameloblastoma.
Abstract Ameloblastoma is a locally aggressive neoplasm with a poorly understood pathogenesis. Therefore, the aim of this study is to investigate whether COX-2 expression is associated with ameloblastoma microvascular density (MVD) and with tumor aggressiveness. Sixty-three cases of primary ameloblastomas arranged in tissue microarray were submitted to immunohistochemistry against cyclooxigenase-2 (COX-2) and CD34. Clinicopathological parameters regarding sex, age, tumour size, tumour duration, tumour location, treatment, recurrences, radiographic features, vestibular/lingual and basal cortical disruption and foll...
Source: Pathology, Research and Practice - March 15, 2018 Category: Pathology Authors: Montezuma MAP, Fonseca FP, Benites BM, Soares CD, do Amaral-Silva GK, de Almeida OP, Soares FA, Pagano RL, Fregnani ER Tags: Pathol Res Pract Source Type: research

miR-486-5p functions as an oncogene by targeting PTEN in non-small cell lung cancer.
CONCLUSIONS: miR-486-5p acts as an oncogene in the progression of NSCLC by influencing PTEN-PI3 K/AKT signaling. miR-486-5p may provide potential therapeutic targets for NSCLC. PMID: 29567332 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - March 15, 2018 Category: Pathology Authors: Gao ZJ, Yuan WD, Yuan JQ, Yuan K, Wang Y Tags: Pathol Res Pract Source Type: research

Chinese herb medicine matrine induce apoptosis in human esophageal squamous cancer KYSE-150 cells through increasing reactive oxygen species and inhibiting mitochondrial function.
Abstract Matrine, as a natural alkaloid isolated from the traditional herb medicine sophora flavescens, has been proved to possess excellent biological activities, including anticancer effects. Now, this research aims to assess the anticancer activities and the mechanism of matrine against esophageal cancer cells, we investigated the proliferative inhibition, apoptosis induction, as well as the underlying mechanism of matrine on esophageal cancer KYSE-150 cells. It was found that matrine could suppress KYSE-150 cell proliferation and significantly mediate cell apoptosis in a dose-dependent relation by increasing i...
Source: Pathology, Research and Practice - March 15, 2018 Category: Pathology Authors: Jiang JH, Pi J, Jin H, Yang F, Cai JY Tags: Pathol Res Pract Source Type: research

Smad4/Fascin index is highly prognostic in patients with diffuse type EBV-associated gastric cancer.
Abstract Gastric cancer is a heterogeneous disorder for which predicting clinical outcomes is challenging, although various biomarkers have been suggested. The Smad4 and Fascin proteins are known prognostic indicators of different types of malignancy. Smad4 primarily functions as a key regulator of tumor suppression, whereas Fascin exhibits oncogenic function by enhancing tumor infiltration. A combined marker based on these opposing roles may improve prognostic accuracy in gastric cancer. Smad4 and Fascin expression was assessed in tissue microarrays obtained from 285 primary gastric adenocarcinoma, 201 normal tis...
Source: Pathology, Research and Practice - March 15, 2018 Category: Pathology Authors: Son BK, Kim DH, Min KW, Kim EK, Kwon MJ Tags: Pathol Res Pract Source Type: research

Corrigendum to "Cytological and histological changes in the urothelium produced by electromotive drug administration (EMDA) and by the combination of intravescical hyperthermia and chemotherapy (thermochemotherapy)" [Pathol. Res. Pract. 213 (2017) 1078-1081].
Corrigendum to "Cytological and histological changes in the urothelium produced by electromotive drug administration (EMDA) and by the combination of intravescical hyperthermia and chemotherapy (thermochemotherapy)" [Pathol. Res. Pract. 213 (2017) 1078-1081]. Pathol Res Pract. 2018 Mar 14;: Authors: Pierconti F, Straccia P, Emilio S, Bassi PF, De Pascalis I, Marques RC, Volavsek M, Larocca LM, Lopez-Beltran A PMID: 29549984 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - March 14, 2018 Category: Pathology Authors: Pierconti F, Straccia P, Emilio S, Bassi PF, De Pascalis I, Marques RC, Volavsek M, Larocca LM, Lopez-Beltran A Tags: Pathol Res Pract Source Type: research

Intracystic papillary carcinoma of the breast: Experience of a major Chinese cancer center.
CONCLUSION: Overall, we report some unique features of IPC in the Chinese population. The patients of IPC of the breast in China have more excellent prognosis than in Caucasian and other races. The diagnostic accuracy of imaging was low, and was easily misinterpreted as a cyst or benign disease. Preoperative core needle biopsies are very difficult for accurate pathological diagnosis of IPC. IPC is a localized disease with a low frequency of axillary lymph node involvement, rare distant metastases and excellent survival. The low incidence of axillary lymph node metastasis suggests that quadrectomy + sentinel lymph node ...
Source: Pathology, Research and Practice - March 8, 2018 Category: Pathology Authors: Zhang J, Zhang T, Wu N, Zhao X, Wang Q, Jiang Y, Gao M, Gu L Tags: Pathol Res Pract Source Type: research

High TRIM44 expression in endometrial carcinoma is associated with a poorer patient outcome.
Abstract BACKGROUND: Tripartite motif-containing protein 44 (TRIM44) has been recently identified as a novel oncogene that is overexpressed in several types of human cancers; however, its role in endometrial cancer (EC) remains unknown. The purpose of the current study was to investigate the TRIM44 protein expression and clinicopathological significance of TRIM44 in EC. METHODS: Paraffin-embedded surgical specimens were collected from 143 patients with EC for the immunohistochemical analysis of TRIM44 expression. Western blotting was performed to evaluate differences in TRIM44 protein expression in EC and nor...
Source: Pathology, Research and Practice - March 6, 2018 Category: Pathology Authors: Li P, Yin H, Meng F, Liu S, Liu H, Ma R Tags: Pathol Res Pract Source Type: research

Association between NME1 polymorphisms and cancer susceptibility: A meta-analysis based on 1644 cases and 2038 controls.
Abstract The association between polymorphisms in the nucleoside diphosphate kinase 1 (NME1) gene and overall risk of cancer remains to be elucidated. Here, we performed a meta-analysis of the association between rs16949649, rs2302254, and rs34214448 polymorphisms in the NME1 gene and cancer risk. PubMed, Web of Science, and CNKI databases (as of June 6, 2017) were searched. Eight studies, encompassing 1644 cases and 2038 controls, were selected. The results revealed no significant relationship between NME1 polymorphisms and overall cancer susceptibility. Interestingly, the rs16949649 polymorphism was associated w...
Source: Pathology, Research and Practice - March 6, 2018 Category: Pathology Authors: Shi X, Jin H, Peng M, Li B, She M, Zhu T, Wen S, Qin D Tags: Pathol Res Pract Source Type: research

Expression of miR-200c and its clinicopathological significance in patients with colorectal cancer.
We examined the association of miR-200c expression with various clinicopathological factors, including KRAS mutation status and survival, in patients with colorectal cancer (CRC). The expression level of miR-200c was evaluated in 109 paired CRC and normal tissue samples using quantitative reverse transcription polymerase chain reaction. The KRAS mutation status of the CRC samples was determined using the PNAClamp™ KRAS Mutation Detection kit. Compared with the normal tissue group, miR-200c expression was significantly upregulated in the CRCs (P 
Source: Pathology, Research and Practice - February 26, 2018 Category: Pathology Authors: Roh MS, Lee HW, Jung SB, Kim K, Lee EH, Park MI, Lee JS, Kim MS Tags: Pathol Res Pract Source Type: research

An immunohistochemical panel consisting of EZH2, C-KIT, and CD205 is useful for distinguishing thymic squamous cell carcinoma from type B3 thymoma.
Abstract Type B3 thymoma and thymic squamous cell carcinoma (SqCC) often cause a diagnostic problem due to their histological similarities. The aim of this study is to identify EZH2 as a novel and powerful biomarker that can effectively distinguish thymic SqCC from type B3 thymoma, and find optimal combinations among 11 markers. A total of 53 patients, comprising 26 with type B3 thymoma and 27 with thymic SqCC, were allocated to the discovery or validation cohorts, and immunohistochemical staining was performed and analyzed. The expression level of each marker was scored, and receiver-operator characteristic curve...
Source: Pathology, Research and Practice - February 24, 2018 Category: Pathology Authors: Kim BS, Kim JK, Kang CH, Kim YT, Jung KC, Won JK Tags: Pathol Res Pract Source Type: research

Loss of p27kip1 expression is associated with poor prognosis in patients with taxane-treated breast cancer.
CONCLUSIONS: Low p27kip1 expression may be useful to predict overall survival in patients with breast cancer who are treated with taxane. Evaluation of p27kip1 expression may provide further prognostic information beyond traditional prognostic biomarkers and an understanding of the mechanisms that impart resistance against chemotherapy. PMID: 29482985 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - February 14, 2018 Category: Pathology Authors: Kim GJ, Kim DH, Min KW, Kim YH, Oh YH Tags: Pathol Res Pract Source Type: research

Immunohistochemical evaluation of H3K27 trimethylation in malignant peripheral nerve sheath tumors.
Abstract The histological definitive diagnosis of malignant peripheral nerve sheath tumor (MPNST) is quite difficult because the morphological features are not specific and no useful immunohistochemical marker has been identified. Loss-of-function mutations in EED or SUZ12, which encode the core subunit of polycomb repressive complex 2 (PRC2), were reported in MPNSTs, and the mutations were shown to cause inactivation of PRC2, leading to loss of trimethylation of histone H3 at lysine 27 (H3K27me3). Immunohistochemistry of H3K27me3 is expected to be a specific marker for MPNSTs. We evaluated immunohistochemical exp...
Source: Pathology, Research and Practice - January 31, 2018 Category: Pathology Authors: Otsuka H, Kohashi K, Yoshimoto M, Ishihara S, Toda Y, Yamada Y, Yamamoto H, Nakashima Y, Oda Y Tags: Pathol Res Pract Source Type: research

Hyalinizing clear cell carcinoma of salivary glands: A retrospective study focused on uncommon morphology, immunohistochemistry, and detection of gene fusion using fluorescence in situ hybridization.
CONCLUSIONS: Our study has expanded the morphological spectrum of HCCC, and proposed the diagnosis of HCCC should be confirmed by fully analyzing histological, immunohistochemical, and molecular features practically. PMID: 29482986 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - January 3, 2018 Category: Pathology Authors: Zhao YN, Wang X, Liang FH, Zhang WJ, Song XT Tags: Pathol Res Pract Source Type: research

Whole-exome sequencing of chondroid hamartoma of lung identified no driver mutations.
PMID: 29482990 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - January 2, 2018 Category: Pathology Authors: Choi SH, Park HC, Kim MS, Chung YJ, Lee SH Tags: Pathol Res Pract Source Type: research

Clinicopathologic features of adult EBV-associated B-cell lymphoproliferative disease.
In conclusion, the present study shows that polymorphous morphology and detection of necrosis in AELPD are frequently linked to a fatal clinical course, whereas Hodgkin-like morphology seems to be associated with a more favourable prognosis. Clonal rearrangement of IgH or TCR is frequent in AELPD, but prognosis is unpredictable from this feature. PMID: 29258765 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - December 16, 2017 Category: Pathology Authors: Wörner S, Mueller-Hermelink HK, Voelker HU Tags: Pathol Res Pract Source Type: research

Immunohistochemical comparative analysis of GFAP, MAP - 2, NOGO - A, OLIG - 2 and WT - 1 expression in WHO 2016 classified neuroepithelial tumours and their prognostic value.
Abstract Immunohistochemistry is routinely used in differential diagnosis of tumours of the central nervous system (CNS). The latest 2016 WHO 2016 revision now includes molecular data such as IDH mutation and 1p/19q codeletion thus restructuring glioma classification. Direct comparative information between commonly used immunohistochemical markers for glial tumours GFAP, MAP - 2, NOGO - A, OLIG - 2 and WT - 1 concerning quality and quantity of expression and their relation to the new molecular markers are lacking. We therefore compared the immunohistochemical staining results of all five antibodies in 34 oligodend...
Source: Pathology, Research and Practice - December 14, 2017 Category: Pathology Authors: Schwab DE, Lepski G, Borchers C, Trautmann K, Paulsen F, Schittenhelm J Tags: Pathol Res Pract Source Type: research

Frequent methylation of DAB2, a Wnt pathway antagonist, in oral and oropharyngeal squamous cell carcinomas.
CONCLUSIONS: Our findings corroborate that DAB2 is a frequent target of epigenetic silencing in oral carcinomas and may be potentially used for tumor detection. PMID: 29254794 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - December 14, 2017 Category: Pathology Authors: Paluszczak J, Kiwerska K, Mielcarek-Kuchta D Tags: Pathol Res Pract Source Type: research

Gallic acid modulates phenotypic behavior and gene expression in oral squamous cell carcinoma cells by interfering with leptin pathway.
, Farias LC Abstract Gallic acid is a polyphenolic compost appointed to interfere with neoplastic cells behavior. Evidence suggests an important role of leptin in carcinogenesis pathways, inducing a proliferative phenotype. We investigated the potential of gallic acid to modulate leptin-induced cell proliferation and migration of oral squamous cell carcinoma cell lines. The gallic acid effect on leptin secretion by oral squamous cell carcinoma cells, as well as the underlying molecular mechanisms, was also assessed. For this, we performed proliferation, migration, immunocytochemical and qPCR assays. The expression...
Source: Pathology, Research and Practice - December 10, 2017 Category: Pathology Authors: Santos EMS, da Rocha RG, Santos HO, Guimarães TA, de Carvalho Fraga CA, da Silveira LH, Batista PR, de Oliveira PSL, Melo GA, Santos SH, de Paula AMB, Guimarães ALS, Farias LC Tags: Pathol Res Pract Source Type: research

Expression profiles of Annexin A1, formylated peptide receptors and cyclooxigenase-2 in gastroesophageal inflammations and neoplasias.
ol AP Abstract The anti-inflammatory protein Annexin-A1 (ANXA1) is associated to tumor invasion process and its actions can be mediated by formylated peptides receptors (FPRs). Therefore, we evaluated the expression and correlation of ANXA1, FPR and cyclooxygenase-2 (COX-2) enzyme in esophageal and stomach inflammations and neoplasias. The study of proteins was performed by immunohistochemistry in biopsies of esophagitis, Barrett's esophagus, squamous cell carcinoma and adenocarcinoma of the esophagus, as well as gastritis, stomach polypus and gastric adenocarcinoma. The intensity of the expressions was evaluated ...
Source: Pathology, Research and Practice - December 7, 2017 Category: Pathology Authors: Takaoka RTC, Sertório ND, Magalini LPJ, Dos Santos LM, Souza HR, Iyomasa-Pilon MM, Possebon L, Costa SS, Girol AP Tags: Pathol Res Pract Source Type: research

Prognostic value of GRIM-19, NF- κB and IKK2 in patients with high-grade serous ovarian cancer.
CONCLUSIONS: GRIM-19 is a potential predictor of prognosis and disease recurrence in HGSC. IKK2 and NF-κB are related to poor prognosis and are potential predictors of response to platinum-based chemotherapy in HGSC. IHC analyses of GRIM19, IKK2 and NF-κB may be important in the attempt to provide prognostic values for relapse and response to treatment in patients with HGSC. PMID: 29254797 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - December 6, 2017 Category: Pathology Authors: Ilelis F, do Amaral NS, Alves MR, da Costa AABA, Calsavara VF, Lordello L, De Brot L, Soares FA, Rodrigues IS, Rocha RM Tags: Pathol Res Pract Source Type: research

Bone marrow miR-10a overexpression is associated with genetic events but not affects clinical outcome in acute myeloid leukemia.
CONCLUSIONS: BM miR-10 overexpression is associated with genetic events but not affects clinical outcome in AML. PMID: 29254789 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - December 5, 2017 Category: Pathology Authors: Zhang TJ, Guo H, Zhou JD, Li XX, Zhang W, Ma JC, Wen XM, Yao XY, Lin J, Qian J Tags: Pathol Res Pract Source Type: research

Immunophenotypic profile of tumor buds in breast cancer.
CONCLUSION: Most tumor buds (96.5%) share the same HR and Her2 expression profile of the main tumor mass, implying that tumor buds relay on the same pathways as the main tumor mass and might be equally responsive to targeted therapies. PMID: 29254793 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - December 5, 2017 Category: Pathology Authors: Laedrach C, Salhia B, Cihoric N, Zlobec I, Tapia C Tags: Pathol Res Pract Source Type: research

Downregulation of miR-10B* is correlated with altered expression of mitotic kinases in osteosarcoma.
Abstract Dysregulated mitotic kinases have frequently been associated with cancer. Changes in their expression might result from diverse mechanisms including avoidance of the tight regulation exerted by miRNAs. Herein we show that miR-10b* is downregulated in osteosarcoma samples and demonstrate its correlation with PLK1, PLK4, BUB1, and BUBR1, which are strongly intercorrelated. The selection of miRNAs that coordinately target and regulate multiple members of cancer-related pathways are particularly advantageous to tumors. Thus, even though no associations with clinical parameters were found, our data place miR-1...
Source: Pathology, Research and Practice - December 2, 2017 Category: Pathology Authors: Roberto GM, Engel EE, Scrideli CA, Tone LG, Brassesco MS Tags: Pathol Res Pract Source Type: research

Corrigendum to "Characterization of common and rare mutations in EGFR and associated clinicopathological features in a large population of Chinese patients with lung cancer" [Pathology - Research and Practice 213 (2017) 749-758].
Corrigendum to "Characterization of common and rare mutations in EGFR and associated clinicopathological features in a large population of Chinese patients with lung cancer" [Pathology - Research and Practice 213 (2017) 749-758]. Pathol Res Pract. 2017 Dec;213(12):1575 Authors: Wei B, Ren P, Zhang C, Wang Z, Dong B, Yang K, Zhao J, Tu S, Ma J, Guo Y PMID: 29167027 [PubMed - in process] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - November 25, 2017 Category: Pathology Authors: Wei B, Ren P, Zhang C, Wang Z, Dong B, Yang K, Zhao J, Tu S, Ma J, Guo Y Tags: Pathol Res Pract Source Type: research

Corrigendum to "Validation of a next generation sequencing panel for detection of hotspot cancer mutations in a clinical laboratory" [Journal information: Pathology-Research and Practice, 2017, Volume 213, Issue 2, Pages: 98-105].
Corrigendum to "Validation of a next generation sequencing panel for detection of hotspot cancer mutations in a clinical laboratory" [Journal information: Pathology-Research and Practice, 2017, Volume 213, Issue 2, Pages: 98-105]. Pathol Res Pract. 2017 Dec;213(12):1576 Authors: Shahsiah R, DeKoning J, Samie S, Latifzadeh SZ, Kashi ZM PMID: 29167028 [PubMed - in process] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - November 25, 2017 Category: Pathology Authors: Shahsiah R, DeKoning J, Samie S, Latifzadeh SZ, Kashi ZM Tags: Pathol Res Pract Source Type: research

Osteoclast-like giant cells in invasive breast cancer predominantly possess M2-macrophage phenotype.
We described the clinicopathological characteristics of 8 patients with breast carcinoma with OGCs. Immuno-phenotypes of the OGCs were comparatively examined with that of foreign body giant cells (FBGCs) in 4 cases of granulomatous reaction (GR) without cancerous elements. In most cancers, tumors displayed cribriform and tubular growth patterns. Three cases showed moderate to high nuclear grade, while all the other tumors had low nuclear grade. Six patients were estrogen receptor (ER) positive, while triple negative phenotype was identified in 2 patients. During the follow-up period, 1 patient had local recurrence of the t...
Source: Pathology, Research and Practice - November 9, 2017 Category: Pathology Authors: Ohashi R, Yanagihara K, Namimatsu S, Sakatani T, Takei H, Naito Z, Shimizu A Tags: Pathol Res Pract Source Type: research

Prognostic roles of lymph node micrometastasis in non-small cell lung cancer.
CONCLUSION: Taken together, our results showed that LNMM was detected in 25.3% of NSCLCs without nodal disease through ancillary test. In addition, the presence of LNMM was significantly correlated with a higher recurrence rate and worse survival rates in early-stage NSCLC. PMID: 29129492 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - November 8, 2017 Category: Pathology Authors: Jeong JH, Kim NY, Pyo JS Tags: Pathol Res Pract Source Type: research