Digital image analysis of pan-cytokeratin stained tumor slides for evaluation of tumor budding in pT1/pT2 colorectal cancer: Results of a feasibility study.
is LB Abstract Tumor budding is an independent prognostic factor in colorectal cancer. However, varying degrees of interobserver agreement and reproducibility challenges the use of tumor budding in diagnostics. Immunohistochemical staining of tumor slides with pan-cytokeratin visualizes the budding tumor cells and has been suggested to improve reproducibility. Here we demonstrate the methodology of tumor budding assessment using digital image analysis based on tumor slides stained for pan-cytokeratin, and investigate interobserver agreement, agreement between manual and digital assessment methods and digital repro...
Source: Pathology, Research and Practice - July 10, 2018 Category: Pathology Authors: Jepsen RK, Klarskov LL, Lippert MF, Novotny GW, Hansen TP, Christensen IJ, Høgdall E, Riis LB Tags: Pathol Res Pract Source Type: research

Loss of LINC01939 expression predicts progression and poor survival in gastric cancer.
In this study, we identified a novel lncRNA LINC01939 which showed the largest fold change in GC than other human cancers from lnCAR database by bioinformatic analysis. Reverse transcription quantitative polymerase chain reaction (qPCR) assay confirmed that LINC01939 was significantly downregulated in GC tissues compared with matched normal tissues. Low expression of LINC01939 was positively associated with advanced TNM stage and lymphatic metastasis. Patients with low LINC01939 expression have remarkably shorter overall survival (OS) and progression-free survival (PFS) than those with high LINC01939 expression. Univariate...
Source: Pathology, Research and Practice - July 10, 2018 Category: Pathology Authors: Chen CL, Ke Q, Luo M, Gao ZY, Li ZJ, Luo ZG, Liu DB Tags: Pathol Res Pract Source Type: research

The function of long non-coding RNA MT1JP in the development and progression of gastric cancer.
Abstract Gastric cancer is one of the most common malignant tumors with the third mortality rate of cancer in world. A larger number of researches showed that genetic factors played an important role in the development of gastric cancer. More and more evidences have indicated that lncRNAs can regulate the gene expression through the transcription, post transcription and epigenetic levels including cancer invasion and metastasis, cell differentiation and apoptosis. However, the mechanisms of lncRNAs in the pathogenesis of gastric cancer remaining unclear. LncRNA MT1JP overexpression vector was constructed and then ...
Source: Pathology, Research and Practice - July 7, 2018 Category: Pathology Authors: Lv Z, Zhang Y, Yu X, Lin Y, Ge Y Tags: Pathol Res Pract Source Type: research

An extremely rare case of primary malignancy in giant cell tumor of bone, arising in the right femur and harboring H3F3A mutation.
Abstract We experienced a case of primary malignancy in giant cell tumor of bone (GCTB), arising in the right femur and harboring H3F3A mutation. A 27-year-old Japanese male without any prior disease history complained of pain in his right hip joint and right lower limb. Radiological images revealed an osteolytic and multicystic lesion existing mainly at the proximal epiphysis of the right femur. Preoperative clinical diagnosis was GCTB, although irregular marginal sclerosis was an atypical radiographic finding for conventional GCTBs. Biopsy sample from the lesion revealed the coexistence of typical GCTB and undif...
Source: Pathology, Research and Practice - June 28, 2018 Category: Pathology Authors: Tsukamoto Y, Futani H, Kihara T, Watanabe T, Kumanishi S, Matsuo S, Hirota S, Ueda T, Yamamoto H, Yoshiya S Tags: Pathol Res Pract Source Type: research

Increased number of arginase 1-positive cells in the stroma of carcinomas compared to precursor lesions and nonneoplastic tissues.
We examined the Arg1-positive cells in tumor microenvironment of gastric carcinomas (GCs), colorectal carcinomas (CRCs) and prostate carcinomas (PCs), and analyzed their clinicopathological significance. Immunohistochemical staining for Arg1 was done in 60 GCs, 38 gastric adenomas, 40 CRCs, 10 colonic adenomas, 36 PCs, and 15 benign prostatic hyperplasia (BPH). Arg1 expression was predominantly localized in tumor microenvironment and the stroma of nonneoplastic tissues. Cells with Arg1 expression were mostly leukocytes, morphologically resembling polymorphonuclear neutrophils, and showed CD15 expression. Arg1 expression wa...
Source: Pathology, Research and Practice - June 28, 2018 Category: Pathology Authors: Jang TJ, Kim SA, Kim MK Tags: Pathol Res Pract Source Type: research

The regulatory role of Nrf2 in antioxidants phase2 enzymes and IL-17A expression in patients with ulcerative colitis.
CONCLUSION: Oxidative stress in inflamed colonic tissue can induce Nrf2 gene expression. The performance of Nrf2 transcription factor may lead to the induction of GST-A4 and PRDX1. IL-17A is less detected in intestinal inflammation, presenting Nrf2 factor. The present findings suggest that Nrf2 function in the gut plays a role in arresting both inflammatory response and oxidative damages of UC. PMID: 29941222 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - June 22, 2018 Category: Pathology Authors: Sabzevary-Ghahfarokhi M, Shohan M, Shirzad H, Rahimian G, Soltani A, Ghatreh-Samani M, Deris F, Bagheri N, Shafigh M, Tahmasbi K Tags: Pathol Res Pract Source Type: research

Microvessel density and angiogenesis in primary hepatic malignancies: Differential expression of CD31 and VEGFR-2 in hepatocellular carcinoma and intrahepatic cholangiocarcinoma.
Fend F Abstract BACKGROUND: Microvessel density is an indicator of tumor-driven neoangiogenesis. Hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC) have distinct vascular patterns, which are also reflected in their imaging characteristics. Since a significant proportion of HCC are treated without biopsy confirmation, it is essential to discriminate HCC and ICC radiologically. The aim of our study was therefore to compare microvessel density and expression of VEGFR-2 in HCC and ICC, since these data may ultimately help us to better understand their imaging characteristics. Whereas CD31 docume...
Source: Pathology, Research and Practice - June 19, 2018 Category: Pathology Authors: Bösmüller H, Pfefferle V, Bittar Z, Scheble V, Horger M, Sipos B, Fend F Tags: Pathol Res Pract Source Type: research

Disparate genomic characteristics of concurrent endometrial adenocarcinoma and ovarian granulosa cell tumor, revealed by targeted next-generation sequencing.
Abstract Concurrence of both endometrial adenocarcinoma and ovarian adult granulosa cell tumor (aGCT) is believed to be related to high estrogen milieu, but genomic alterations of the concurrent endometrial adenocarcinoma and aGCT are not known. For this, we analyzed an uterine endometrial adenocarcinoma and an ovarian aGCT in a same patient by a targeted next generation sequencing (NGS). We found a germline mutation in STK11 (p.L113fs). The endometrial adenocarcinoma harbored FGFR2 and TP53 mutations and the aGCT harbored a FOXL2 (p.C134 W) mutation. These germline and somatic mutations have been reported in no...
Source: Pathology, Research and Practice - June 19, 2018 Category: Pathology Authors: Choi YJ, Ho J, Lee A, Hur SY, Park HC, Park JS, Chung YJ, Lee SH Tags: Pathol Res Pract Source Type: research

Knockdown of TRIM66 inhibits malignant behavior and epithelial-mesenchymal transition in non-small cell lung cancer.
CONCLUSION: Silence TRIM66 expression suppressed NSCLC cell proliferation, invasion, and migration. The siRNA-mediated TRIM66 silencing could block the occurrence of EMT. TRIM66 could be a promising novel target for future NSCLC treatments. PMID: 29929749 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - June 18, 2018 Category: Pathology Authors: Dai HY, Ma Y, Da Z, Hou XM Tags: Pathol Res Pract Source Type: research

Low levels of ADAM23 expression in epithelial ovarian cancer are associated with poor survival.
CONCLUSIONS: Our results demonstrate that ADAM23 expression is likely involved in the progression of EOC and may provide potential diagnostic and prognostic information regarding EOC. PMID: 29921495 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - June 12, 2018 Category: Pathology Authors: Ma R, Tang Z, Sun K, Ye X, Cheng H, Chang X, Cui H Tags: Pathol Res Pract Source Type: research

Potential impact of PD-L1 (SP-142) immunohistochemical heterogeneity in clear cell renal cell carcinoma immunotherapy.
, Lawrie CH Abstract Intratumor heterogeneity (ITH) detection remains a challenge in modern oncology because it can have a direct impact on the success of new therapies. Anti-PD-1/PD-L1 immunotherapy is an emerging treatment modality that is showing great promise for clear cell renal cell carcinoma (CCRCC) patients with advanced disease. Patient selection for such therapy relies upon the immunohistochemical detection of PD-1/PD-L1, however the degree of ITH for these markers among tumor cells and/or inflammatory mononuclear infiltrates remains unknown. Therefore, we analyzed PD-L1 (SP-142) expression in the tumor ...
Source: Pathology, Research and Practice - June 12, 2018 Category: Pathology Authors: López JI, Pulido R, Cortés JM, Angulo JC, Lawrie CH Tags: Pathol Res Pract Source Type: research

Clinicopathological and prognostic significance of C/EBP homologous protein (CHOP) in advanced gastric cancer.
Abstract BACKGROUND: Few studies have reported the clinical and prognostic significance of C/EBP homologous protein (CHOP) in advanced gastric cancer (GC). Therefore, the present study investigated the expression of CHOP in advanced GC patients to determine its potential prognostic role. METHODS: The levels of CHOP in 95 patients with advanced GC and adjacent non-cancerous tissues were evaluated by qRT-PCR, western blot and immunohistochemistry. Furthermore, the association of CHOP expression with clinicopathological parameters and prognosis of advanced GC patients was analyzed. RESULTS: The levels of CH...
Source: Pathology, Research and Practice - June 12, 2018 Category: Pathology Authors: Zhang X, Zhou T, Li W, Zhang T, Che N, Zu G Tags: Pathol Res Pract Source Type: research

The expression of IMP3 in 366 cases with ovarian carcinoma of high grade serous, endometrioid and clear cell subtypes.
Abstract The clear cell (CCC), high grade serous (HGSC) and endometrioid (EC) ovarian carcinomas share overlapping histological features. The oncogene IMP3 is implicated in CCC with an elusive utility in differential diagnosis. We collected 366 cases with ovarian primary carcinomas to detect IMP3, Napsin-A and HNF-1β by immunochemistry. In 351 cases, the positive expression rate of IMP3 in CCC was significantly higher than that either in EC or HGSC (p 
Source: Pathology, Research and Practice - May 29, 2018 Category: Pathology Authors: Lu L, Wang S, Zhu Q, Qu Y, Gu W, Ning Y, Chen X, Wang Y Tags: Pathol Res Pract Source Type: research

Clinical characteristics of primary intestinal NK/T cell lymphoma, nasal type: Case series and review of the literature.
CONCLUSION: Intestinal NK/T cell lymphoma shows nonspecific clinical features and poor prognosis, which is mainly expressed as ulcers on endoscopy. Emergency surgery may be an adverse prognostic factor of lymphoma, since it is prone to progress toward gastrointestinal perforation. PMID: 29853314 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 25, 2018 Category: Pathology Authors: Yang L, Tang X, Peng X, Qian D, Guo Q, Guo H Tags: Pathol Res Pract Source Type: research

Down-regulation of microRNA-27b promotes retinal pigment epithelial cell proliferation and migration by targeting Nox2.
In this study, we found that the expression level of miR-27b was significantly reduced in ARPE-19 cells under PDGF-BB stimulation. Ectopic expression of miR-27b remarkably inhibited PDGF-BB-induced proliferation and migration in ARPE-19 cells. Furthermore, bioinformatic analysis and luciferase reporter assay showed that NADPH oxidase 2 (Nox2) was a direct target for miR-27b, and that knockdown of Nox2 expression mimicked the inhibitory effect of miR-27b on PDGF-BB -induced proliferation and migration in ARPE-19 cells, whereas, restoration of Nox2 expression showed an opposite effect. In addition, the ROS production and the...
Source: Pathology, Research and Practice - May 24, 2018 Category: Pathology Authors: Li J, Hui L, Kang Q, Li R Tags: Pathol Res Pract Source Type: research

Lanthanum deposition corresponds to white lesions in the stomach.
Abstract OBJECTIVE: Although lanthanum deposition in the stomach has been most frequently reported to occur as white lesions, no study has investigated whether the white lesions observed during esophagogastroduodenoscopy are truly lanthanum-related. Here, we retrospectively investigated the amount of lanthanum in endoscopic biopsy specimens. METHODS: We reviewed four patients showing gastric white spots or annular whitish mucosa in the gastric white lesions (Bw) and peripheral mucosa where the white substance was not endoscopically observed (Bp) during biopsy. We also reviewed three patients with diffuse whit...
Source: Pathology, Research and Practice - May 23, 2018 Category: Pathology Authors: Iwamuro M, Urata H, Tanaka T, Kawano S, Kawahara Y, Kimoto K, Okada H Tags: Pathol Res Pract Source Type: research

Extranodal NK/T-cell lymphoma of the nasal cavity developed in a patient with intestinal Epstein-Barr virus-positive T/NK-cell lymphoproliferative disorder.
Abstract Extranodal NK/T cell lymphoma, nasal type (ENKL) developed in a patient with intestinal Epstein-Barr virus (EBV)-positive T/NK-cell lymphoproliferative disorder (LPD). The patient was a 46-year-old Chinese man who complained of diarrhea and abdominal pain without immune-deficiency. Endoscopy demonstrated ileum ulcers like Crohn's disease, without histological granulomas. His symptoms continued waxing and waning for 3 years until he developed overt lymphoma (ENKL) in the nasal cavity. The ileum lesions exacerbated into a large deep ulcer, and the biopsy specimens from the ileum, including the one 3 years a...
Source: Pathology, Research and Practice - May 22, 2018 Category: Pathology Authors: Furuya G, Abe H, Shinozaki-Ushiku A, Yamashita A, Ihara S, Hirata Y, Chiba A, Fujioka Y, Kurokawa M, Koike K, Fukayama M Tags: Pathol Res Pract Source Type: research

High NUSAP1 expression predicts poor prognosis in colon cancer.
In this study, we investigated the clinical significance of NUSAP1 expression in colon cancer. METHODS AND MATERIALS: Immunohistochemical staining was performed to determine NUSAP1 protein levels in paraffin colon tumor specimens. Real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) was conducted to detect NUSAP1 mRNA levels in colon tumor samples. The association between NUSAP1 protein expression and clinicopathological characteristics of patients with colon cancer was assessed. A Kaplan-Meier analysis was performed to determine the prognostic significance of NUSAP1 in colon cancer. A Cox proportion...
Source: Pathology, Research and Practice - May 22, 2018 Category: Pathology Authors: Liu Z, Guan C, Lu C, Liu Y, Ni R, Xiao M, Bian Z Tags: Pathol Res Pract Source Type: research

High expression of QSOX1 is associated with tumor invasiveness and high grades groups in prostate cancer.
Abstract Prostate cancer is the most common malignancy in men, and biologically shows highly heterogeneous clinical outcomes, despite early detection. Therefore, the identification of novel molecular markers that are associated with biological aggressiveness is very important for prostatic cancer clinical outcome predictions and treatment choices. Here, we investigate quiescin sulfhydryl oxidase 1 (QSOX1) expression and evaluate its clinicopathological significance and prognostic impact in prostate cancers, with immunohistochemistry on tissue microarrays. QSOX1 over-expression was observed in 12 (11.2%) of prostat...
Source: Pathology, Research and Practice - May 22, 2018 Category: Pathology Authors: Baek JA, Song PH, Ko Y, Gu MJ Tags: Pathol Res Pract Source Type: research

Endobronchial ultrasound-guided transbronchial needle aspiration in the diagnosis of mediastinal metastases of clear cell renal cell carcinoma.
Abstract Evaluation of mediastinal lymphadenopathy in patients with a previous diagnosis of renal cell carcinoma (RCC) is critical for the determination of further treatment. A minimally invasive method of cytology sampling of mediastinal lymph nodes using endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) has emerged as a useful tool in diagnosis. Between January 2010 and April 2018, we performed 1744 EBUS-TBNA studies of mediastinal and hilar lymph nodes for a variety of clinical indications including mediastinal malignancy. Sixteen patients (93.7% males, mean age 59.1 years, range 44-8...
Source: Pathology, Research and Practice - May 22, 2018 Category: Pathology Authors: Val-Bernal JF, Martino M, Romay F, Yllera E Tags: Pathol Res Pract Source Type: research

The prognostic value of DAPK1 hypermethylation in gliomas: A site-specific analysis.
Abstract BACKGROUND AND AIMS: The gene of death associated protein kinase 1 (DAPK1) has been reported to be methylated in various cancers including gliomas. However, its prognostic value for gliomas is still controversy, and the methylation at specific CpG sites of DAPK1 has not been investigated. The aim of this study was to prognostically evaluate the methylation level of different CpG sites within DAPK1 promoter region in gliomas. METHODS: Based on sodium bisulfite treated DNA products, we made use of DNA pyrosequencing method to evaluate overall and site-specific methylation of DAPK1 in 143 gliomas and 26...
Source: Pathology, Research and Practice - May 22, 2018 Category: Pathology Authors: Li X, Pu J, Liu J, Chen Y, Li Y, Hou P, Shi B, Yang Q Tags: Pathol Res Pract Source Type: research

Multi-laboratory proficiency testing of clinical cancer genomic profiling by next-generation sequencing.
In conclusion, this study strongly highlights the importance of establishing standards for NGS-based testing, particularly when the test results impact on clinical decisions, and systematically provides data sets from multiple different labs to infer such standards. PMID: 29807778 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 22, 2018 Category: Pathology Authors: Zhong Q, Wagner U, Kurt H, Molinari F, Cathomas G, Komminoth P, Barman-Aksözen J, Schneider-Yin X, Rey JP, Vassella E, Rogel U, Diebold J, McKee T, Jochum W, Kashofer K, Hofman P, Zischka M, Moch H, Rechsteiner M, Wild PJ Tags: Pathol Res Pract Source Type: research

IL-1 β/IL-6 network in the tumor microenvironment of human colorectal cancer.
CONCLUSION: The IL-1β/IL-6 network is highly expressed in the CRC microenvironment, indicating that this network is important in the progression of CRC. PMID: 29803656 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 20, 2018 Category: Pathology Authors: Cui G, Yuan A, Sun Z, Zheng W, Pang Z Tags: Pathol Res Pract Source Type: research

The prognostic values of tumor-infiltrating neutrophils, lymphocytes and neutrophil/lymphocyte rates in bladder urothelial cancer.
Abstract Tumor-infiltrating neutrophils (TINs) and lymphocytes (TILs) are found to play essential roles in many tumors and associate with the prognosis of patients. But, the prognostic values of TINs, TILs and NLR (neutrophils-lymphocytes ratio) in bladder cancer (BC) are still undefined. The object of our study was to systematically interrogate the associations of these immune cells with clinical outcomes of BC patients. In our study, a total of 102 patients pathologically diagnosed with BC were included. CD66b+ and CD8+ antibodies were used to mark neutrophils and CD8+ lymphocytes by immunohistochemistry. The re...
Source: Pathology, Research and Practice - May 20, 2018 Category: Pathology Authors: Liu K, Zhao K, Wang L, Sun E Tags: Pathol Res Pract Source Type: research

H3K27me3 and VEGF is associated with poor prognosis in patients with synovial sarcoma.
This study aimed to investigate the association of H3K27me3 and VEGF expression with clinical outcomes of synovial sarcoma patients. METHODS: This study included 48 patients with synovial sarcoma. H3K27me3 and VEGF levels were evaluated by immunohistochemical staining, and their correlation with clinical parameters was analyzed by Spearman's and Pearson's test. Univariate and multivariate Cox regression analyses were used to identify potential prognostic factors. Kaplan-Meier method was used to analyze overall survival. RESULTS: Protein levels of both H3K27me3 and VEGF were significantly associated with histologi...
Source: Pathology, Research and Practice - May 20, 2018 Category: Pathology Authors: Diao Y, Zhang P, Dai R, Xu J, Feng H Tags: Pathol Res Pract Source Type: research

Cell adhesion molecule profiles, proliferation activity and p53 expression in advanced epithelial ovarian cancer induced malignant ascites-Correlation of tissue microarray and cytology microarray.
CONCLUSION: Epithelial-mesenchymal transition- mesenchymal-epithelial transition is suggested as the best descriptive term for our IHC observations which accompany increased proliferative activity of ascitic cells. The CMA method is an adequate and reliable method for the analysis of ascitic tumor cells disseminating from ovarian malignancies. PMID: 29801775 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 17, 2018 Category: Pathology Authors: Toth L, Nagy B, Mehes G, Laszlo E, Molnar PP, Poka R, Hernadi Z Tags: Pathol Res Pract Source Type: research

Histological assessment & use of immunohistochemical markers for detection of dysplasia in Barrett's esophageal mucosa.
CONCLUSIONS: A detail histological scoring system may bring uniformity in histological interpretation of dysplasia in BE. Using a combined panel of IHC stains seems helpful in detection of dysplasia in BE, especially to differentiate the IFD and LGD changes in BE. PMID: 29764708 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 13, 2018 Category: Pathology Authors: Kinra P, Gahlot GPS, Yadav R, Baloda V, Makharia GK, Gupta SD, Das P Tags: Pathol Res Pract Source Type: research

Long-term prognostic impact of osteopontin and Dickkopf-related protein 1 in patients with hepatocellular carcinoma after hepatectomy.
CONCLUSIONS: Serum OPN and DKK1 levels of HCC patients could be considered as novel biomarkers showing prognostic significance after hepatectomy based on long-term survival data. PMID: 29753515 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 6, 2018 Category: Pathology Authors: Byeon H, Lee SD, Hong EK, Lee DE, Kim BH, Seo Y, Joo J, Han SS, Kim SH, Park SJ Tags: Pathol Res Pract Source Type: research

Comprehensive expression analysis of TNF-related apoptosis-inducing ligand and its receptors in colorectal cancer: Correlation with MAPK alterations and clinicopathological associations.
Abstract TNF-related, apoptosis-inducing ligand (TRAIL) apoptotic pathway constitutes a promising therapeutic target due to high selectivity and low toxicity of TRAIL targeting agents when administered in combination therapies. 106 colorectal cancers were examined for: relative mRNA expression of TRAIL pathway genes, decoy receptors TRAIL-R3 and TRAIL-R4 promoter methylation and the presence of KRAS, NRAS, BRAF mutations. Elevated mRNA levels were observed in 26%, 15%, 13%, 12% and 10% of the cases for TRAIL-R4, TRAIL-R3, TRAIL-R2, TRAIL-R1 and TRAIL genes respectively. Reduced mRNA levels were detected in 77%, 65...
Source: Pathology, Research and Practice - May 1, 2018 Category: Pathology Authors: Tsikalakis S, Chatziandreou I, Michalopoulos NV, Theodoropoulos GE, Sakellariou S, Korkolopoulou P, Patsouris E, Saetta AA Tags: Pathol Res Pract Source Type: research

MiR-21 up-regulation in ampullary adenocarcinoma and its pre-invasive lesions.
Abstract Poor information is available on the molecular landscape characterizing the carcinogenetic process leading to ampullary carcinoma. MiR-21 is one of the most frequently up-regulated miRNAs in pancreatic adenocarcinoma, a tumor sharing similar molecular features with ampullary adenocarcinomas (AVCs), above all with the pancreatic-biliary type. We profiled, by in situ hybridization (ISH), miR-21 expression in a series of 26 AVCs, 50 ampullary dysplastic lesions (35 low-grade [LG-IEN] and 15 high-grade [HG-IEN]) and 10 normal duodenal mucosa samples. The same series was investigated by immunohistochemistry fo...
Source: Pathology, Research and Practice - May 1, 2018 Category: Pathology Authors: Saraggi D, Galuppini F, Fanelli GN, Remo A, Urso EDL, Bao RQ, Bacchin D, Guzzardo V, Luchini C, Braconi C, Farinati F, Rugge M, Fassan M Tags: Pathol Res Pract Source Type: research

Proteins of the retinoblastoma pathway, FEN1 and MGMT are novel potential prognostic biomarkers in pancreatic adenocarcinoma.
CONCLUSIONS: Major cell-cycle regulator also have predictive significance, but further studies are required to evaluate this. PMID: 29735403 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 1, 2018 Category: Pathology Authors: Isohookana J, Haapasaari KM, Soini Y, Leppänen J, Karihtala P Tags: Pathol Res Pract Source Type: research

Prognostic value of EGFR family expression in lymph node-negative esophageal squamous cell carcinoma patients.
This study was designed to determine the prognostic value of EGFR family expression in a population of lymph node-negative ESCC patients treated with curative resection. EGFR family protein expression was examined by immunohistochemical analysis of tissue microarrays of 94 patients with lymph node-negative ESCC after radical esophagectomy with three-field lymphadenectomy. Survival differences were compared using Kaplan-Meier analysis. Cox regression analyses were performed to determine the prognostic factors for overall survival and disease-free survival (DFS). ErbB4 expression was found to be an independent prognostic fac...
Source: Pathology, Research and Practice - April 30, 2018 Category: Pathology Authors: Yu W, Yang X, Chu L, Zhao K, Chen H, Xiang J, Zhang Y, Li H, Zhao W, Sun M, Wei Q, Fu X, Xie C, Zhu Z Tags: Pathol Res Pract Source Type: research

Expression of neuroendocrine differentiation markers in lethal metastatic castration-resistant prostate cancer.
In this study, we studied expression of three commonly used markers of NED (chromogranin A, neuron specific enolase and synaptophysin) in 89 metastases from 31 men that died of castration-resistant prostate cancer and underwent rapid autopsy, and in 89 hormone-naïve primary tumors removed by radical prostatectomy. In addition, we examined NED association with androgen receptor, ERG and Ki-67 expression in metastatic tumor sites. Morphologically, 1 of 31 cases was classified as small cell carcinoma, and the remaining 30 were classified as usual prostate adenocarcinoma using a recently proposed classification of prostat...
Source: Pathology, Research and Practice - April 26, 2018 Category: Pathology Authors: Sainio M, Visakorpi T, Tolonen T, Ilvesaro J, Bova GS Tags: Pathol Res Pract Source Type: research

LncRNA-DANCR: A valuable cancer related long non-coding RNA for human cancers.
CONCLUSIONS: Long non-coding RNAs-DANCR likely serves as a useful disease biomarker or therapeutic cancer target. PMID: 29728310 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 24, 2018 Category: Pathology Authors: Thin KZ, Liu X, Feng X, Raveendran S, Tu JC Tags: Pathol Res Pract Source Type: research

Upregulation of LncRNA FEZF-AS1 is associated with advanced clinical stages and family history of cancer in patients with NSCLC.
Abstract Antisense RNA (AS) is a type of long non-coding RNAs that functions as a post-transcriptional regulatory element on regulating parental coding gene expression via directly binding to complementary mRNA sequences. We aimed to investigate the effect of the AS to FEZF1 gene on non-small cell lung cancer (NSCLC) development. The expression level of lncRNA FEZF-AS1 and FEZF1 was determined by the quantitative Real-time PCR in 160 cases of NSCLC tissues and their adjacent non-tumour tissues. We found that lncRNA FEZF-AS1 was significantly up-regulated in tumour tissues when compared to the adjacent non-cancerou...
Source: Pathology, Research and Practice - April 24, 2018 Category: Pathology Authors: Gong W, Cao Y, Wang Y, Yang L, Su W, Qiu F, Datta S, Rao B, Xian J, Lin M, Feng Y, Zhang X, Zhou Y, Gao X, Lu J Tags: Pathol Res Pract Source Type: research

Intravenous leiomyomatosis of the uterus: A clinicopathologic analysis of 13 cases with an emphasis on histogenesis.
Abstract The object of this study is to investigate the prognosis and potential histologic origin of uterine intravenous leiomyomatosis (IVL). We retrospectively analyzed the clinicopathologic and immunohistochemical features of 13 cases of IVL from a single institute. The patients underwent hysterectomy (3 with bilateral salpingo-oophorectomy, and 8 with excision of the broad ligaments and/or parametrium). They survived with no evidence of disease for 6-90 months. Intravascular worm-like plugs were macroscopically identified in the myometrium in 10 of 13 cases (76.9%). Six patients (46.1%) had extra-uterine invol...
Source: Pathology, Research and Practice - April 22, 2018 Category: Pathology Authors: Tang L, Lu B Tags: Pathol Res Pract Source Type: research

Evaluation of specific modified histones in lip carcinogenesis.
CONCLUSIONS: Histone modifications are related to initial actinic damage, but not to malignant transformation in the lip. PMID: 29699903 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 22, 2018 Category: Pathology Authors: de Freitas Filho SAJ, Servato JPS, de Sá RT, Siqueira CS, de Faria PR, Loyola AM, Cardoso SV Tags: Pathol Res Pract Source Type: research

Tumorigenic role of YAP in hepatocellular carcinogenesis is involved in SHP2 whose function is different in vitro and in vivo.
This study aimed to investigate the tumorigenic roles of YAP and SHP2 in hepatocellular carcinogenesis. Cell density associated subcellular distributions of YAP and SHP2 in normal human hepatocytes (THLE-2) and hepatocellular carcinoma (HCC) cells (SK-Hep1, SNU-182) were investigated by Western blotting and cell block immunohistochemistry. The effects of YAP knockdown on proliferation, migration and invasion were studied using YAP-specific siRNAs. The prognostic significance of YAP and SHP2 expressions was investigated immunohistochemically using a tissue microarray (TMA) from 50 HCC cases. High-cell density decreased the ...
Source: Pathology, Research and Practice - April 21, 2018 Category: Pathology Authors: Kim MK, Park JY, Kang YN Tags: Pathol Res Pract Source Type: research

ALDH1 and tumor infiltrating lymphocytes as predictors for neoadjuvant chemotherapy response in breast cancer.
Abstract Many studies have reported that Aldehyde dehydrogenase 1 (ALDH1) and tumor-infiltrating lymphocytes (TIL) are related to breast cancer prognosis. However, the clinical significance of ALDH1 and tumor-infiltrating immune cells in breast cancer has not been fully investigated in patients who received neoadjuvant chemotherapy (NAC). We studied the significance of the expression of ALDH1 and the population of TIL for predicting the prognosis and chemotherapeutic response of patients with breast cancer who had received NAC. Forty patients who underwent NAC were enrolled in this study. ALDH1 and TIL (T cells an...
Source: Pathology, Research and Practice - April 18, 2018 Category: Pathology Authors: Lee A, Won KY, Lim SJ, Cho SY, Han SA, Park S, Song JY Tags: Pathol Res Pract Source Type: research

The SMYD3 VNTR 3/3 polymorphism confers an increased risk and poor prognosis of hepatocellular carcinoma in a Chinese population.
CONCLUSION: This study indicates that drinking history, family history of HCC and SMYD3 VNTR 3/3, TNM staging, maximum tumor diameter, LNM might be risk factors for HCC, and SMYD3 VNTR 3/3 might contribute to a lower 2- and 3-year survival rate of patients with HCC. PMID: 29691085 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 17, 2018 Category: Pathology Authors: Li RD, Tang YH, Wang HL, Yang D, Sun LJ, Li W Tags: Pathol Res Pract Source Type: research

In silico analysis of the potential mechanism of telocinobufagin on breast cancer MCF-7 cells.
CONCLUSIONS: Telocinobufagin might regulate neuroactive ligand-receptor interaction pathway to exert its influences in breast cancer MCF-7 cells, and its molecular mechanism might share some similarities with fenspiride. This study only presented a comprehensive picture of the role of telocinobufagin in breast cancer MCF-7 cells using big data. However, more thorough and deeper researches are required to add to the validity of this study. PMID: 29656985 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 5, 2018 Category: Pathology Authors: Dang YW, Lin P, Liu LM, He RQ, Zhang LJ, Peng ZG, Li XJ, Chen G Tags: Pathol Res Pract Source Type: research

Clinical implications of TERT promoter mutation on IDH mutation and MGMT promoter methylation in diffuse gliomas.
In conclusion, the combination of TERTp with IDH or MGMT status may be a prognostic indicator depending on grades. PMID: 29650441 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 5, 2018 Category: Pathology Authors: Kim HS, Kwon MJ, Song JH, Kim ES, Kim HY, Min KW Tags: Pathol Res Pract Source Type: research

Investigation of miR-136-5p key target genes and pathways in lung squamous cell cancer based on TCGA database and bioinformatics analysis.
CONCLUSIONS: Downregulated miR-136-5p may target UGT1A7 and ADH7 and participate in ascorbate and aldarate metabolism, pentose and glucuronate interconversions, and retinol metabolism. High expression of UGT1A7 and ADH7 may indicate better prognosis of LUSC patients. PMID: 29650443 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 5, 2018 Category: Pathology Authors: Xie ZC, Li TT, Gan BL, Gao X, Gao L, Chen G, Hu XH Tags: Pathol Res Pract Source Type: research

STR1720, a potential sensitizer for radiotherapy and cytotoxicity effects of NVB-BEZ235 in metastatic breast cancer cells.
Abstract BACKGROUND: Chemo-radio therapy (CRT) resistance is a main barrier in treating the triple negative breast cancer (TNBC). The success of conventional treatment may be ameliorated by elevating the responsiveness of the cancer cells to CRT. NVP-BEZ235 as a PI3K/AKT/mTOR dual inhibitor has been shown promising results in treating breast cancer cells. However, potential radiation-sensitizing effect of NVP-BEZ235 in TNBC remained unclear. In addition, SIRT-1 activation state and environmental cytokine were identified as being responsible for cancer cells responses to CRT. Herein, we investigate the role of inte...
Source: Pathology, Research and Practice - April 5, 2018 Category: Pathology Authors: Fatehi D, Soltani A, Ghatrehsamani M Tags: Pathol Res Pract Source Type: research

Histopathology of locally advanced colorectal carcinoma, with emphasis on tumor invasion of adherent peritoneal membranes.
Abstract Locally advanced colorectal carcinomas are characterized by neoplastic cells that invade beyond the colon and directly into surrounding organs and structures that include the retroperitoneum and abdominopelvic sidewall. These aggressive tumors are prognostically adverse and are categorized with highest possible tumor stage in current cancer staging systems. Recognizing colorectal carcinoma with extensive locoregional invasion is typically straightforward, but some cases can be diagnostically challenging. These include tumors with limited invasion of extracolonic tissue such as the subserosa in which there...
Source: Pathology, Research and Practice - April 3, 2018 Category: Pathology Authors: Chen JH Tags: Pathol Res Pract Source Type: research

Lower expression of bone marrow miR-122 is an independent risk factor for overall survival in cytogenetically normal acute myeloid leukemia.
CONCLUSIONS: Our findings indicated that lower expression of BM miR-122 acted as an independent risk factor for OS in CN-AML. PMID: 29627222 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - April 3, 2018 Category: Pathology Authors: Zhang TJ, Qian Z, Wen XM, Zhou JD, Li XX, Xu ZJ, Ma JC, Zhang ZH, Lin J, Qian J Tags: Pathol Res Pract Source Type: research

Use of the Ion AmpliSeq Cancer Hotspot Panel in clinical molecular pathology laboratories for analysis of solid tumours: With emphasis on validation with relevant single molecular pathology tests and the Oncomine Focus Assay.
Abstract Targeted application of next-generation sequencing (NGS) technology allows detection of specific mutations that can provide treatment opportunities for cancer patients. We evaluated the applicability of the Ion AmpliSeq Cancer Hotspot Panel V2 (CHV2) using formalin-fixed, paraffin-embedded (FFPE) tissue of clinical specimens. Thirty-five FFPE tumour samples with known mutational status were collected from four different hospitals and sequenced with CHV2 using an Ion Chef System and Ion S5 XL system. Out of 35 cases, seven were sequenced with Oncomine focus Assay Panel for comparison. For the limit of dete...
Source: Pathology, Research and Practice - March 29, 2018 Category: Pathology Authors: Lee A, Lee SH, Jung CK, Park G, Lee KY, Choi HJ, Min KO, Kim TJ, Lee EJ, Lee YS Tags: Pathol Res Pract Source Type: research

Immunohistochemical and molecular genetic study on epithelioid glioblastoma: Series of seven cases with review of literature.
Abstract Epithelioid glioblastoma (e-gbm) is a recently described variant of glioblastoma (GBM) which is associated with short survival and now added as a provisional entity to WHO 2016 classification of CNStumors. About half of these tumors show characteristic BRAF-V600E mutation. However, unlike conventional GBMs, e-gbm lack specific diagnostic and prognostic markers. Hence, we aimed to molecularly characterize these tumors. An extensive review of literature was performed.In a multi-institutional effort, all the cases of glioblastoma of year 2017 were reviewed. Cases with predominant epithelioid morphology were ...
Source: Pathology, Research and Practice - March 22, 2018 Category: Pathology Authors: Khanna G, Pathak P, Suri V, Sharma MC, Chaturvedi S, Ahuja A, Bhardwaj M, Garg A, Sarkar C, Sharma R Tags: Pathol Res Pract Source Type: research

Intrahepatic cholangiocarcinoma after Fontan procedure in an adult with visceral heterotaxy.
We report a unique case of intrahepatic cholangiocarcinoma 28 years after a Fontan procedure in a 31year old female with heterotaxy syndrome. The subcapsular mass-forming tumor was composed of poorly differentiated tumor cells arranged in small vague glandular or slit-lumen nests, and focally fused or anastomosing large trabecular patterns within the prominent fibrotic stroma. The tumor cells with immunoreactivity to CK7, CK19, Cam5.2, COX2, EMA, BCL-2, MOC-31 and AE1/AE3, supported a diagnosis of intrahepatic cholangiocarcinoma. Focal atypical ductular proliferation within the background liver may represent a precursor le...
Source: Pathology, Research and Practice - March 15, 2018 Category: Pathology Authors: Wang D, Marshall D, Veldtman G, Gupta A, Trout AT, Villafane J, Bove K Tags: Pathol Res Pract Source Type: research

COX-2 as a determinant of lower disease-free survival for patients affected by ameloblastoma.
Abstract Ameloblastoma is a locally aggressive neoplasm with a poorly understood pathogenesis. Therefore, the aim of this study is to investigate whether COX-2 expression is associated with ameloblastoma microvascular density (MVD) and with tumor aggressiveness. Sixty-three cases of primary ameloblastomas arranged in tissue microarray were submitted to immunohistochemistry against cyclooxigenase-2 (COX-2) and CD34. Clinicopathological parameters regarding sex, age, tumour size, tumour duration, tumour location, treatment, recurrences, radiographic features, vestibular/lingual and basal cortical disruption and foll...
Source: Pathology, Research and Practice - March 15, 2018 Category: Pathology Authors: Montezuma MAP, Fonseca FP, Benites BM, Soares CD, do Amaral-Silva GK, de Almeida OP, Soares FA, Pagano RL, Fregnani ER Tags: Pathol Res Pract Source Type: research