Knockdown of HOXC6 inhibits glioma cell proliferation and induces cell cycle arrest by targeting WIF-1 in vitro and vivo.
CONCLUSIONS: In conclusion, the results of the current study indicate that HOXC6 promotes glioma U87 cell growth through the WIF-1/Wnt signaling pathway and HOXC6 might be a novel target in clinical treatment for gliomas. PMID: 30228024 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - September 12, 2018 Category: Pathology Authors: Yan TF, Wu MJ, Xiao B, Hu Q, Fan YH, Zhu XG Tags: Pathol Res Pract Source Type: research

Human papillomavirus in tonsillectomy specimen from China and Pakistan - Prevalence and genotype distribution.
CONCLUSION: A low prevalence of HPV was found in China and Pakistan; high-risk and low-risk HPV were detectable in tonsillar tissue from both countries. Age stratification ( 25 years) suggest that sexual and non-sexual transmission of the virus can occur. The difference in the genotype distribution geographically within China and with Pakistan was observed in the case of HPV 58. The most common type in both the countries was HPV 16. PMID: 30217437 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - September 6, 2018 Category: Pathology Authors: Mujtaba H, Wang Y, Duan Y, Cao M, Zhang N, Batool I, Murtaza A, Chen X, Wang Y Tags: Pathol Res Pract Source Type: research

Gender-related differences in the association between concomitant amplification of AIB1 and HER2 and clinical outcomes in glioma patients.
Abstract BACKGROUND: Previous studies demonstrated that AIB1 or HER2 copy number gain (CNG), respectively, were independent predictors for poor prognosis of glioma patients, especially in females. We hypothesize that there are some connections between the two genes and sex-specific characteristics, thus this study aimed to analyze gender-related differences in the prognosis of glioma patients. METHODS: Using Real-Time Quantitative Reverse Transcription PCR (RT-qPCR) method, we examined AIB1 and HER2 CNG in gliomas samples (n = 114), and inspected the correlation of various genotypes with patients outcomes...
Source: Pathology, Research and Practice - August 30, 2018 Category: Pathology Authors: Sui F, Sun W, Su X, Chen P, Hou P, Shi B, Yang Q Tags: Pathol Res Pract Source Type: research

Serum CK 18-M30 reflect liver pathological severity during NAFLD progression in a rat model.
CONCLUSION: Our results suggested the significance of CK 18-M30 in the progression of NAFLD and provided new evidence for the early diagnosis and prognostic estimation of NAFLD. PMID: 30149902 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - August 20, 2018 Category: Pathology Authors: Xue L, Lu X, He J, Zhang T, Wu X, Zhang Y, Wang N, An Z, Xu J, Geng Y Tags: Pathol Res Pract Source Type: research

Poorly differentiated osteoclast-like giant cell variant of cutaneous squamous cell carcinoma: Uncovering its mutational landscape through massive parallel sequencing.
PMID: 30146254 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - August 19, 2018 Category: Pathology Authors: Ahmadi Moghaddam P, Singh R, Mahmoodi M, Mehrotra M, Benaim G, Luthra R, Paniz-Mondolfi A Tags: Pathol Res Pract Source Type: research

Effect of anthracycline and taxane on the expression of programmed cell death ligand-1 and galectin-9 in triple-negative breast cancer.
This study identified chemotherapeutic agents that up-regulate programmed cell death ligand-1 (PD-L1) and galectin-9 (Gal-9) in breast cancer cells. Immunohistochemical (IHC) staining was used to evaluate changes in PD-L1 and Gal-9 expression in the tumor tissue of triple-negative breast cancer (TNBC) patients who received anthracycline- and taxane-based neoadjuvant chemotherapy. To determine whether PD-L1 and Gal-9 expression changes were attributable directly to chemotherapeutics, MDA-MB-231 cells and HS578T cells were treated with different concentrations of anthracycline and taxane. Expression levels of PD-L1 and Gal-9...
Source: Pathology, Research and Practice - August 17, 2018 Category: Pathology Authors: Yoon HK, Kim TH, Park S, Jung H, Quan X, Park SJ, Han J, Lee A Tags: Pathol Res Pract Source Type: research

A new approach for Alzheimer's disease treatment through P-gp regulation via ibuprofen.
In conclusion, the P-gp expression and function were suppressed in AD condition by activating inflammatory reaction, and then causing the Aβ efflux decreased. However, upregulating P-gp could increase the Aβ efflux in further to treat AD via inhibiting the inflammatory factors expression. PMID: 30139557 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - August 17, 2018 Category: Pathology Authors: Zhang C, Qin H, Zheng R, Wang Y, Yan T, Huan F, Han Y, Zhu W, Zhang L Tags: Pathol Res Pract Source Type: research

Association between loss of heterozygosity of chromosome 16q and survival in Wilms' tumor: A meta-analysis.
This study aimed to performed a meta-analysis to clarify this. METHODS: Databases including the Wanfang, PubMed, Chinese National Knowledge Infrastructure, Embase, and Cochrane Library databases were searched July 2018. The meta-analysis was done using Stata (version 14.0). Publication bias was evaluated by funnel plots, Begg's test, and Egger's test. The trim-and-fill method was applied if significant publication bias existed. Sensitivity analysis was performed to evaluate the stability of the results. RESULTS: This meta-analysis identified 9 cohort studies encompassing 3266 cases. The pooled relative risk when ...
Source: Pathology, Research and Practice - August 17, 2018 Category: Pathology Authors: Bu Q, He H, Fan D, Lyu J, Pan Z, You H Tags: Pathol Res Pract Source Type: research

Overexpression of UQCRC2 is correlated with tumor progression and poor prognosis in colorectal cancer.
Abstract Ubiquinol-cytochrome c reductase complex core protein 2 (UQCRC2) is an important subunit of mitochondrial respiratory complex III. However, its role in tumorigenesis and tumor progression remains unknown, especially with regards to colorectal cancer (CRC). In this research, we measured the expression of UQCRC2 protein by immunohistochemistry assay in 89 paired paraffin-embedded tumor tissues and corresponding adjacent normal tissues from patients with colorectal adenocarcinoma and investigated possible correlations of UQCRC2 expression with clinicopathological parameters and prognosis. We found that UQCRC...
Source: Pathology, Research and Practice - August 11, 2018 Category: Pathology Authors: Shang Y, Zhang F, Li D, Li C, Li H, Jiang Y, Zhang D Tags: Pathol Res Pract Source Type: research

Possible mechanisms mediating apoptosis of bronchial epithelial cells in chronic obstructive pulmonary disease - A next-generation sequencing approach.
CONCLUSION: Our findings suggest PI3K-Akt signaling pathway plays an important role in COPD. We observed altered expression of apoptosis and cell proliferation-related genes that might contribute to the pathogenesis of COPD. PMID: 30115538 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - August 7, 2018 Category: Pathology Authors: Tsai MJ, Chang WA, Jian SF, Chang KF, Sheu CC, Kuo PL Tags: Pathol Res Pract Source Type: research

Correlation between adenosine triphosphate (ATP)-binding cassette transporter G2 (ABCG2) and drug resistance of esophageal cancer and reversal of drug resistance by artesunate.
Abstract The present study investigated the correlation between the abnormal expression of adenosine triphosphate (ATP)-binding cassette transporter G2 (ABCG2) in esophageal cancer and the drug resistance of esophageal cancer, the reversal effect in drug resistance of artesunate (Art), and the mechanism underlying esophageal cancer using nude mice with subcutaneous xenograft as an animal model. The gene and protein expression of ABCG2 was detected in 80 cases of esophageal cancer, atypical dysplasia, and normal mucosa. A subcutaneous xenograft tumor mouse model was established by subcutaneous inoculation of esopha...
Source: Pathology, Research and Practice - August 7, 2018 Category: Pathology Authors: Wang L, Liu L, Chen Y, Du Y, Wang J, Liu J Tags: Pathol Res Pract Source Type: research

Long non-coding RNAs expression levels in diffuse large B-cell lymphoma: An in silico analysis.
In this study, the expression levels of 189 approved lncRNAs were considered in DLBCL patients using several different genomic and transcriptome datasets. The analyses showed that the lncRNA GAS5 allocated the maximum score of RNA dysregulation and can be considered as good choice in DLBCLs' researches. PMID: 30104077 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - August 7, 2018 Category: Pathology Authors: Dousti F, Shahrisa A, Ansari H, Hajjari M, Tahmasebi Birgani Y, Mohammadiasl J, Tahmasebi Birgani M Tags: Pathol Res Pract Source Type: research

Chondroblastoma-like tumor of the skull in a patient with cardio-facio-cutaneous syndrome.
Abstract Cardio-facio-cutaneous syndrome (CFCS) is a rare genetic disorder characterized by craniofacial deformities and heterogeneous cardiac and cutaneous manifestations. The condition is caused by de novo activating mutations in one of four genes encoding proteins involved in the RAS-MAPK signaling pathway; specifically BRAF, MEK1, MEK2, or KRAS. Variable malignancies have been reported in patients with CFCS. Herein we report a chondroblastoma-like lesion of the skull in a 20-year-old man with a clinical diagnosis of CFCS and a long-standing history of medically intractable epilepsy. Patients with CFCS have pre...
Source: Pathology, Research and Practice - August 7, 2018 Category: Pathology Authors: Fasciano D, Wei S, Li R, Siegal GP Tags: Pathol Res Pract Source Type: research

Antioxidant effects on the intracerebroventricular galactose damage in rats.
Abstract We investigated the effects of the intracerebroventricular infusion of galactose and the influence of pretreatment with antioxidants on oxidative stress parameters and acethylcholinesterase (AChE) activity in the brain of 60-day-old Wistar rats (6 per group). The animals were divided into naïve group (did not undergo surgery); procedure group (only underwent surgery); sham group (underwent surgery and received 5 μL saline) and galactose group (received 5 μL of galactose solution (5.0 mM) by intracerebroventricular injection), and were killed by decapitation after 1 h...
Source: Pathology, Research and Practice - August 3, 2018 Category: Pathology Authors: Sasso S, Cruz IR, Lorenzini MS, Delwing-Dal Magro D, Brueckheimer MB, Maia TP, Sala GABN, Mews MHR, Delwing-de Lima D Tags: Pathol Res Pract Source Type: research

The clinicopathological significance of SIRT1 expression in colon cancer: An immunohistochemical study and meta-analysis.
CONCLUSION: In our retrospective study, high SIRT1 expression significantly correlated with vascular invasion and a worse prognosis. However, because the results from the meta-analysis differed the retrospective arm of our study, additional cumulative studies are needed to determine the prognostic value of SIRT1 in CRC. PMID: 30082156 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - August 1, 2018 Category: Pathology Authors: Hong WG, Pyo JS Tags: Pathol Res Pract Source Type: research

An insight into metastasis: Random or evolving paradigms?
Abstract Mechanical or fostered molecular events define metastatic cascade. Three distinct sets of molecular events characterize metastasis, viz invasion of extracellular matrix; angiogenesis, vascular dissemination and anoikis resistance; tumor homing and relocation of tumor cells to selective organ. Invasion of extracellular matrix requires epithelial to mesenchymal transition through disrupted lamellopodia formation and contraction of actin cytoskeleton; aberration of Focal adhesion complex formation involving integrins and the extracellular matrix; degradation of extracellular matrix by matrix metalloproteases...
Source: Pathology, Research and Practice - August 1, 2018 Category: Pathology Authors: Bhattacharya R, Panda CK, Nandi S, Mukhopadhyay A Tags: Pathol Res Pract Source Type: research

Histopathological changes in the gastroduodenal mucosa of children with functional dyspepsia.
CONCLUSIONS: A significant proportion of children with FD do not show chronic or active inflammation, but have increased mast cell density and eosinophil density in the stomach and duodenum mucosa. Our study adds functional dyspepsia to the list of various abnormalities that have increased gastroduodenal mucosal elevations of eosinophils and/or mast cells. PMID: 30078402 [PubMed - in process] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - August 1, 2018 Category: Pathology Authors: Singh V, Singh M, Schurman JV, Friesen CA Tags: Pathol Res Pract Source Type: research

Calpeptin attenuates cigarette smoke-induced pulmonary inflammation via suppressing calpain/I κBα signaling in mice and BEAS-2B cells.
Calpeptin attenuates cigarette smoke-induced pulmonary inflammation via suppressing calpain/IκBα signaling in mice and BEAS-2B cells. Pathol Res Pract. 2018 Aug;214(8):1199-1209 Authors: Zuo J, Hu Z, Liu T, Chen C, Tao Z, Chen S, Li F Abstract Exposure to cigarette smoke including secondhand smoking is the most important risk factor in the development of chronic obstructive pulmonary disease where incidence has substantially increased in recent decades. The mechanisms responsible for cigarette smoke-induced pulmonary inflammation remain unclear, and thus lack of effective treatment. The pr...
Source: Pathology, Research and Practice - August 1, 2018 Category: Pathology Authors: Zuo J, Hu Z, Liu T, Chen C, Tao Z, Chen S, Li F Tags: Pathol Res Pract Source Type: research

Long non-coding RNAs in the gastric juice of gastric cancer patients.
Abstract Differently from other digestive malignancies, gastric cancer (GC) carcinogenesis seems more heterogeneous and unclear. This entails failing in identification of reliable serum tumor markers for screening early GC (EGC) as well as persisting ominous prognosis of this disease. Recently, investigation of human noncoding genome, especially long noncoding molecules (lncRNAs), has provided promising data. As for GC, however, since the current information on GC-specific lncRNAs is still scarce and comes largely from analyses performed on tissue or serum of affected patients, we decided to review the current lit...
Source: Pathology, Research and Practice - July 27, 2018 Category: Pathology Authors: Virgilio E, Giarnieri E, Giovagnoli MR, Montagnini M, Proietti A, D'Urso R, Mercantini P, Balducci G, Cavallini M Tags: Pathol Res Pract Source Type: research

Vimentin immunohistochemical expression as a prognostic factor in gastric cancer: A meta-analysis.
CONCLUSIONS: Positive vimentin expression could serve as a poor prognostic marker in GC. PMID: 30078472 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - July 25, 2018 Category: Pathology Authors: Yin S, Chen FF, Yang GF Tags: Pathol Res Pract Source Type: research

Mixofibrosarcoma of the kidney: An unusual site for a common soft tissue tumor.
PMID: 30078470 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - July 23, 2018 Category: Pathology Authors: Motta F, Puzzo L, Regis F, Morgia G, Magro G Tags: Pathol Res Pract Source Type: research

STAT6 expression in spindle cell lesions of the breast: An immunohistochemical study of 48 cases.
Abstract The diagnosis of spindle cell lesions of the breast parenchyma is challenging. Some of these lesions share the expression of CD34, posing differential diagnostic problems, especially in core biopsies. Recently, antibodies against the STAT6 C-terminal, are being used in paraffin-embedded tissues as a surrogate for identifying the NAB2-STA6 fusion gene which is considered a specific molecular marker for solitary fibrous tumor. Accordingly, we investigated the expression of STAT6 in a large series of uncommon spindle cell tumor-like and tumor lesions occurring primarily in the breast parenchyma. We collected...
Source: Pathology, Research and Practice - July 17, 2018 Category: Pathology Authors: Magro G, Spadola S, Motta F, Palazzo J, Catalano F, Vecchio GM, Salvatorelli L Tags: Pathol Res Pract Source Type: research

CD3-positive diffuse large B-cell lymphoma relapses as CD3-negative large B-cell lymphoma: Loss of aberrant antigen expression in B-cell lymphoma after chemotherapy.
Abstract Aberrant expression of CD3 on diffuse large B-cell lymphoma (DLBCL) is rare, and its mechanism and biological significance are currently unclear. Herein we report a case of Epstein-Barr virus-negative, CD3-positive DLBCL in a 53 year-old male, who had a remote history of renal transplantation. After standard chemotherapy, the patient was in clinical remission. He relapsed three years later, but at this time with apparent loss of CD3 expression. PCR-based IGK gene rearrangement studies demonstrated clonal amplicons with an identical nucleotide size between the primary and secondary DLBCL, confirming the ...
Source: Pathology, Research and Practice - July 16, 2018 Category: Pathology Authors: Yang LH, Ingersoll K, Zhao Y, Luedke C, Sebastian S, Wang E Tags: Pathol Res Pract Source Type: research

Therapeutic management of peritoneal ascitic sarcomatosis by Ruta graveolens: A study in experimental mice.
CONCLUSION: Our observations revealed that loco-regional Ruta administration resulted in the anti-neoplastic effect on peritoneal sarcoma related ascites and the alteration of vital regulatory molecules which depicted the therapeutic utility of Ruta in the management of peritoneal malignant ascites. PMID: 30025594 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - July 11, 2018 Category: Pathology Authors: Law S, Sanyal S, Chatterjee R, Law A, Law A, Chattopadhyay S Tags: Pathol Res Pract Source Type: research

Prognostic significance of carbonic anhydrase IX overexpression in stage III non-small cell lung cancer patients after neoadjuvant treatment.
Abstract BACKGROUND: To assess the prognostic importance of carbonic anhydrase IX (CA IX), a hypoxic biomarker, after neoadjuvant treatment in Stage III non-small cell lung cancer (NSCLC) patients. METHODS: Tissue CA IX expression was examined after surgical resection in 77 patients who had undergone neoadjuvant treatment. The effects of CA IX overexpression and other clinical factors on disease-free survival and overall survival were investigated. RESULTS: In multivariate analysis, number of neoadjuvant chemotherapy (CT) courses and gender emerged as significant independent predictors for disease-free s...
Source: Pathology, Research and Practice - July 11, 2018 Category: Pathology Authors: Ibis K, Saglam S, Saglam EK, Firat P, Yilmazbayhan D, Toker A, Ozkan B, Hancer VS, Buyukdogan M, Disci R, Pilanci KN Tags: Pathol Res Pract Source Type: research

Digital image analysis of pan-cytokeratin stained tumor slides for evaluation of tumor budding in pT1/pT2 colorectal cancer: Results of a feasibility study.
is LB Abstract Tumor budding is an independent prognostic factor in colorectal cancer. However, varying degrees of interobserver agreement and reproducibility challenges the use of tumor budding in diagnostics. Immunohistochemical staining of tumor slides with pan-cytokeratin visualizes the budding tumor cells and has been suggested to improve reproducibility. Here we demonstrate the methodology of tumor budding assessment using digital image analysis based on tumor slides stained for pan-cytokeratin, and investigate interobserver agreement, agreement between manual and digital assessment methods and digital repro...
Source: Pathology, Research and Practice - July 10, 2018 Category: Pathology Authors: Jepsen RK, Klarskov LL, Lippert MF, Novotny GW, Hansen TP, Christensen IJ, Høgdall E, Riis LB Tags: Pathol Res Pract Source Type: research

Loss of LINC01939 expression predicts progression and poor survival in gastric cancer.
In this study, we identified a novel lncRNA LINC01939 which showed the largest fold change in GC than other human cancers from lnCAR database by bioinformatic analysis. Reverse transcription quantitative polymerase chain reaction (qPCR) assay confirmed that LINC01939 was significantly downregulated in GC tissues compared with matched normal tissues. Low expression of LINC01939 was positively associated with advanced TNM stage and lymphatic metastasis. Patients with low LINC01939 expression have remarkably shorter overall survival (OS) and progression-free survival (PFS) than those with high LINC01939 expression. Univariate...
Source: Pathology, Research and Practice - July 10, 2018 Category: Pathology Authors: Chen CL, Ke Q, Luo M, Gao ZY, Li ZJ, Luo ZG, Liu DB Tags: Pathol Res Pract Source Type: research

The function of long non-coding RNA MT1JP in the development and progression of gastric cancer.
Abstract Gastric cancer is one of the most common malignant tumors with the third mortality rate of cancer in world. A larger number of researches showed that genetic factors played an important role in the development of gastric cancer. More and more evidences have indicated that lncRNAs can regulate the gene expression through the transcription, post transcription and epigenetic levels including cancer invasion and metastasis, cell differentiation and apoptosis. However, the mechanisms of lncRNAs in the pathogenesis of gastric cancer remaining unclear. LncRNA MT1JP overexpression vector was constructed and then ...
Source: Pathology, Research and Practice - July 7, 2018 Category: Pathology Authors: Lv Z, Zhang Y, Yu X, Lin Y, Ge Y Tags: Pathol Res Pract Source Type: research

An extremely rare case of primary malignancy in giant cell tumor of bone, arising in the right femur and harboring H3F3A mutation.
Abstract We experienced a case of primary malignancy in giant cell tumor of bone (GCTB), arising in the right femur and harboring H3F3A mutation. A 27-year-old Japanese male without any prior disease history complained of pain in his right hip joint and right lower limb. Radiological images revealed an osteolytic and multicystic lesion existing mainly at the proximal epiphysis of the right femur. Preoperative clinical diagnosis was GCTB, although irregular marginal sclerosis was an atypical radiographic finding for conventional GCTBs. Biopsy sample from the lesion revealed the coexistence of typical GCTB and undif...
Source: Pathology, Research and Practice - June 28, 2018 Category: Pathology Authors: Tsukamoto Y, Futani H, Kihara T, Watanabe T, Kumanishi S, Matsuo S, Hirota S, Ueda T, Yamamoto H, Yoshiya S Tags: Pathol Res Pract Source Type: research

Increased number of arginase 1-positive cells in the stroma of carcinomas compared to precursor lesions and nonneoplastic tissues.
We examined the Arg1-positive cells in tumor microenvironment of gastric carcinomas (GCs), colorectal carcinomas (CRCs) and prostate carcinomas (PCs), and analyzed their clinicopathological significance. Immunohistochemical staining for Arg1 was done in 60 GCs, 38 gastric adenomas, 40 CRCs, 10 colonic adenomas, 36 PCs, and 15 benign prostatic hyperplasia (BPH). Arg1 expression was predominantly localized in tumor microenvironment and the stroma of nonneoplastic tissues. Cells with Arg1 expression were mostly leukocytes, morphologically resembling polymorphonuclear neutrophils, and showed CD15 expression. Arg1 expression wa...
Source: Pathology, Research and Practice - June 28, 2018 Category: Pathology Authors: Jang TJ, Kim SA, Kim MK Tags: Pathol Res Pract Source Type: research

The regulatory role of Nrf2 in antioxidants phase2 enzymes and IL-17A expression in patients with ulcerative colitis.
CONCLUSION: Oxidative stress in inflamed colonic tissue can induce Nrf2 gene expression. The performance of Nrf2 transcription factor may lead to the induction of GST-A4 and PRDX1. IL-17A is less detected in intestinal inflammation, presenting Nrf2 factor. The present findings suggest that Nrf2 function in the gut plays a role in arresting both inflammatory response and oxidative damages of UC. PMID: 29941222 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - June 22, 2018 Category: Pathology Authors: Sabzevary-Ghahfarokhi M, Shohan M, Shirzad H, Rahimian G, Soltani A, Ghatreh-Samani M, Deris F, Bagheri N, Shafigh M, Tahmasbi K Tags: Pathol Res Pract Source Type: research

Microvessel density and angiogenesis in primary hepatic malignancies: Differential expression of CD31 and VEGFR-2 in hepatocellular carcinoma and intrahepatic cholangiocarcinoma.
Fend F Abstract BACKGROUND: Microvessel density is an indicator of tumor-driven neoangiogenesis. Hepatocellular carcinoma (HCC) and intrahepatic cholangiocarcinoma (ICC) have distinct vascular patterns, which are also reflected in their imaging characteristics. Since a significant proportion of HCC are treated without biopsy confirmation, it is essential to discriminate HCC and ICC radiologically. The aim of our study was therefore to compare microvessel density and expression of VEGFR-2 in HCC and ICC, since these data may ultimately help us to better understand their imaging characteristics. Whereas CD31 docume...
Source: Pathology, Research and Practice - June 19, 2018 Category: Pathology Authors: Bösmüller H, Pfefferle V, Bittar Z, Scheble V, Horger M, Sipos B, Fend F Tags: Pathol Res Pract Source Type: research

Disparate genomic characteristics of concurrent endometrial adenocarcinoma and ovarian granulosa cell tumor, revealed by targeted next-generation sequencing.
Abstract Concurrence of both endometrial adenocarcinoma and ovarian adult granulosa cell tumor (aGCT) is believed to be related to high estrogen milieu, but genomic alterations of the concurrent endometrial adenocarcinoma and aGCT are not known. For this, we analyzed an uterine endometrial adenocarcinoma and an ovarian aGCT in a same patient by a targeted next generation sequencing (NGS). We found a germline mutation in STK11 (p.L113fs). The endometrial adenocarcinoma harbored FGFR2 and TP53 mutations and the aGCT harbored a FOXL2 (p.C134 W) mutation. These germline and somatic mutations have been reported in no...
Source: Pathology, Research and Practice - June 19, 2018 Category: Pathology Authors: Choi YJ, Ho J, Lee A, Hur SY, Park HC, Park JS, Chung YJ, Lee SH Tags: Pathol Res Pract Source Type: research

Knockdown of TRIM66 inhibits malignant behavior and epithelial-mesenchymal transition in non-small cell lung cancer.
CONCLUSION: Silence TRIM66 expression suppressed NSCLC cell proliferation, invasion, and migration. The siRNA-mediated TRIM66 silencing could block the occurrence of EMT. TRIM66 could be a promising novel target for future NSCLC treatments. PMID: 29929749 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - June 18, 2018 Category: Pathology Authors: Dai HY, Ma Y, Da Z, Hou XM Tags: Pathol Res Pract Source Type: research

Low levels of ADAM23 expression in epithelial ovarian cancer are associated with poor survival.
CONCLUSIONS: Our results demonstrate that ADAM23 expression is likely involved in the progression of EOC and may provide potential diagnostic and prognostic information regarding EOC. PMID: 29921495 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - June 12, 2018 Category: Pathology Authors: Ma R, Tang Z, Sun K, Ye X, Cheng H, Chang X, Cui H Tags: Pathol Res Pract Source Type: research

Potential impact of PD-L1 (SP-142) immunohistochemical heterogeneity in clear cell renal cell carcinoma immunotherapy.
, Lawrie CH Abstract Intratumor heterogeneity (ITH) detection remains a challenge in modern oncology because it can have a direct impact on the success of new therapies. Anti-PD-1/PD-L1 immunotherapy is an emerging treatment modality that is showing great promise for clear cell renal cell carcinoma (CCRCC) patients with advanced disease. Patient selection for such therapy relies upon the immunohistochemical detection of PD-1/PD-L1, however the degree of ITH for these markers among tumor cells and/or inflammatory mononuclear infiltrates remains unknown. Therefore, we analyzed PD-L1 (SP-142) expression in the tumor ...
Source: Pathology, Research and Practice - June 12, 2018 Category: Pathology Authors: López JI, Pulido R, Cortés JM, Angulo JC, Lawrie CH Tags: Pathol Res Pract Source Type: research

Clinicopathological and prognostic significance of C/EBP homologous protein (CHOP) in advanced gastric cancer.
Abstract BACKGROUND: Few studies have reported the clinical and prognostic significance of C/EBP homologous protein (CHOP) in advanced gastric cancer (GC). Therefore, the present study investigated the expression of CHOP in advanced GC patients to determine its potential prognostic role. METHODS: The levels of CHOP in 95 patients with advanced GC and adjacent non-cancerous tissues were evaluated by qRT-PCR, western blot and immunohistochemistry. Furthermore, the association of CHOP expression with clinicopathological parameters and prognosis of advanced GC patients was analyzed. RESULTS: The levels of CH...
Source: Pathology, Research and Practice - June 12, 2018 Category: Pathology Authors: Zhang X, Zhou T, Li W, Zhang T, Che N, Zu G Tags: Pathol Res Pract Source Type: research

The expression of IMP3 in 366 cases with ovarian carcinoma of high grade serous, endometrioid and clear cell subtypes.
Abstract The clear cell (CCC), high grade serous (HGSC) and endometrioid (EC) ovarian carcinomas share overlapping histological features. The oncogene IMP3 is implicated in CCC with an elusive utility in differential diagnosis. We collected 366 cases with ovarian primary carcinomas to detect IMP3, Napsin-A and HNF-1β by immunochemistry. In 351 cases, the positive expression rate of IMP3 in CCC was significantly higher than that either in EC or HGSC (p 
Source: Pathology, Research and Practice - May 29, 2018 Category: Pathology Authors: Lu L, Wang S, Zhu Q, Qu Y, Gu W, Ning Y, Chen X, Wang Y Tags: Pathol Res Pract Source Type: research

Clinical characteristics of primary intestinal NK/T cell lymphoma, nasal type: Case series and review of the literature.
CONCLUSION: Intestinal NK/T cell lymphoma shows nonspecific clinical features and poor prognosis, which is mainly expressed as ulcers on endoscopy. Emergency surgery may be an adverse prognostic factor of lymphoma, since it is prone to progress toward gastrointestinal perforation. PMID: 29853314 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 25, 2018 Category: Pathology Authors: Yang L, Tang X, Peng X, Qian D, Guo Q, Guo H Tags: Pathol Res Pract Source Type: research

Down-regulation of microRNA-27b promotes retinal pigment epithelial cell proliferation and migration by targeting Nox2.
In this study, we found that the expression level of miR-27b was significantly reduced in ARPE-19 cells under PDGF-BB stimulation. Ectopic expression of miR-27b remarkably inhibited PDGF-BB-induced proliferation and migration in ARPE-19 cells. Furthermore, bioinformatic analysis and luciferase reporter assay showed that NADPH oxidase 2 (Nox2) was a direct target for miR-27b, and that knockdown of Nox2 expression mimicked the inhibitory effect of miR-27b on PDGF-BB -induced proliferation and migration in ARPE-19 cells, whereas, restoration of Nox2 expression showed an opposite effect. In addition, the ROS production and the...
Source: Pathology, Research and Practice - May 24, 2018 Category: Pathology Authors: Li J, Hui L, Kang Q, Li R Tags: Pathol Res Pract Source Type: research

Lanthanum deposition corresponds to white lesions in the stomach.
Abstract OBJECTIVE: Although lanthanum deposition in the stomach has been most frequently reported to occur as white lesions, no study has investigated whether the white lesions observed during esophagogastroduodenoscopy are truly lanthanum-related. Here, we retrospectively investigated the amount of lanthanum in endoscopic biopsy specimens. METHODS: We reviewed four patients showing gastric white spots or annular whitish mucosa in the gastric white lesions (Bw) and peripheral mucosa where the white substance was not endoscopically observed (Bp) during biopsy. We also reviewed three patients with diffuse whit...
Source: Pathology, Research and Practice - May 23, 2018 Category: Pathology Authors: Iwamuro M, Urata H, Tanaka T, Kawano S, Kawahara Y, Kimoto K, Okada H Tags: Pathol Res Pract Source Type: research

Extranodal NK/T-cell lymphoma of the nasal cavity developed in a patient with intestinal Epstein-Barr virus-positive T/NK-cell lymphoproliferative disorder.
Abstract Extranodal NK/T cell lymphoma, nasal type (ENKL) developed in a patient with intestinal Epstein-Barr virus (EBV)-positive T/NK-cell lymphoproliferative disorder (LPD). The patient was a 46-year-old Chinese man who complained of diarrhea and abdominal pain without immune-deficiency. Endoscopy demonstrated ileum ulcers like Crohn's disease, without histological granulomas. His symptoms continued waxing and waning for 3 years until he developed overt lymphoma (ENKL) in the nasal cavity. The ileum lesions exacerbated into a large deep ulcer, and the biopsy specimens from the ileum, including the one 3 years a...
Source: Pathology, Research and Practice - May 22, 2018 Category: Pathology Authors: Furuya G, Abe H, Shinozaki-Ushiku A, Yamashita A, Ihara S, Hirata Y, Chiba A, Fujioka Y, Kurokawa M, Koike K, Fukayama M Tags: Pathol Res Pract Source Type: research

High NUSAP1 expression predicts poor prognosis in colon cancer.
In this study, we investigated the clinical significance of NUSAP1 expression in colon cancer. METHODS AND MATERIALS: Immunohistochemical staining was performed to determine NUSAP1 protein levels in paraffin colon tumor specimens. Real-time fluorescent quantitative polymerase chain reaction (RT-qPCR) was conducted to detect NUSAP1 mRNA levels in colon tumor samples. The association between NUSAP1 protein expression and clinicopathological characteristics of patients with colon cancer was assessed. A Kaplan-Meier analysis was performed to determine the prognostic significance of NUSAP1 in colon cancer. A Cox proportion...
Source: Pathology, Research and Practice - May 22, 2018 Category: Pathology Authors: Liu Z, Guan C, Lu C, Liu Y, Ni R, Xiao M, Bian Z Tags: Pathol Res Pract Source Type: research

High expression of QSOX1 is associated with tumor invasiveness and high grades groups in prostate cancer.
Abstract Prostate cancer is the most common malignancy in men, and biologically shows highly heterogeneous clinical outcomes, despite early detection. Therefore, the identification of novel molecular markers that are associated with biological aggressiveness is very important for prostatic cancer clinical outcome predictions and treatment choices. Here, we investigate quiescin sulfhydryl oxidase 1 (QSOX1) expression and evaluate its clinicopathological significance and prognostic impact in prostate cancers, with immunohistochemistry on tissue microarrays. QSOX1 over-expression was observed in 12 (11.2%) of prostat...
Source: Pathology, Research and Practice - May 22, 2018 Category: Pathology Authors: Baek JA, Song PH, Ko Y, Gu MJ Tags: Pathol Res Pract Source Type: research

Endobronchial ultrasound-guided transbronchial needle aspiration in the diagnosis of mediastinal metastases of clear cell renal cell carcinoma.
Abstract Evaluation of mediastinal lymphadenopathy in patients with a previous diagnosis of renal cell carcinoma (RCC) is critical for the determination of further treatment. A minimally invasive method of cytology sampling of mediastinal lymph nodes using endobronchial ultrasound-guided transbronchial needle aspiration (EBUS-TBNA) has emerged as a useful tool in diagnosis. Between January 2010 and April 2018, we performed 1744 EBUS-TBNA studies of mediastinal and hilar lymph nodes for a variety of clinical indications including mediastinal malignancy. Sixteen patients (93.7% males, mean age 59.1 years, range 44-8...
Source: Pathology, Research and Practice - May 22, 2018 Category: Pathology Authors: Val-Bernal JF, Martino M, Romay F, Yllera E Tags: Pathol Res Pract Source Type: research

The prognostic value of DAPK1 hypermethylation in gliomas: A site-specific analysis.
Abstract BACKGROUND AND AIMS: The gene of death associated protein kinase 1 (DAPK1) has been reported to be methylated in various cancers including gliomas. However, its prognostic value for gliomas is still controversy, and the methylation at specific CpG sites of DAPK1 has not been investigated. The aim of this study was to prognostically evaluate the methylation level of different CpG sites within DAPK1 promoter region in gliomas. METHODS: Based on sodium bisulfite treated DNA products, we made use of DNA pyrosequencing method to evaluate overall and site-specific methylation of DAPK1 in 143 gliomas and 26...
Source: Pathology, Research and Practice - May 22, 2018 Category: Pathology Authors: Li X, Pu J, Liu J, Chen Y, Li Y, Hou P, Shi B, Yang Q Tags: Pathol Res Pract Source Type: research

Multi-laboratory proficiency testing of clinical cancer genomic profiling by next-generation sequencing.
In conclusion, this study strongly highlights the importance of establishing standards for NGS-based testing, particularly when the test results impact on clinical decisions, and systematically provides data sets from multiple different labs to infer such standards. PMID: 29807778 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 22, 2018 Category: Pathology Authors: Zhong Q, Wagner U, Kurt H, Molinari F, Cathomas G, Komminoth P, Barman-Aksözen J, Schneider-Yin X, Rey JP, Vassella E, Rogel U, Diebold J, McKee T, Jochum W, Kashofer K, Hofman P, Zischka M, Moch H, Rechsteiner M, Wild PJ Tags: Pathol Res Pract Source Type: research

IL-1 β/IL-6 network in the tumor microenvironment of human colorectal cancer.
CONCLUSION: The IL-1β/IL-6 network is highly expressed in the CRC microenvironment, indicating that this network is important in the progression of CRC. PMID: 29803656 [PubMed - as supplied by publisher] (Source: Pathology, Research and Practice)
Source: Pathology, Research and Practice - May 20, 2018 Category: Pathology Authors: Cui G, Yuan A, Sun Z, Zheng W, Pang Z Tags: Pathol Res Pract Source Type: research

The prognostic values of tumor-infiltrating neutrophils, lymphocytes and neutrophil/lymphocyte rates in bladder urothelial cancer.
Abstract Tumor-infiltrating neutrophils (TINs) and lymphocytes (TILs) are found to play essential roles in many tumors and associate with the prognosis of patients. But, the prognostic values of TINs, TILs and NLR (neutrophils-lymphocytes ratio) in bladder cancer (BC) are still undefined. The object of our study was to systematically interrogate the associations of these immune cells with clinical outcomes of BC patients. In our study, a total of 102 patients pathologically diagnosed with BC were included. CD66b+ and CD8+ antibodies were used to mark neutrophils and CD8+ lymphocytes by immunohistochemistry. The re...
Source: Pathology, Research and Practice - May 20, 2018 Category: Pathology Authors: Liu K, Zhao K, Wang L, Sun E Tags: Pathol Res Pract Source Type: research

H3K27me3 and VEGF is associated with poor prognosis in patients with synovial sarcoma.
This study aimed to investigate the association of H3K27me3 and VEGF expression with clinical outcomes of synovial sarcoma patients. METHODS: This study included 48 patients with synovial sarcoma. H3K27me3 and VEGF levels were evaluated by immunohistochemical staining, and their correlation with clinical parameters was analyzed by Spearman's and Pearson's test. Univariate and multivariate Cox regression analyses were used to identify potential prognostic factors. Kaplan-Meier method was used to analyze overall survival. RESULTS: Protein levels of both H3K27me3 and VEGF were significantly associated with histologi...
Source: Pathology, Research and Practice - May 20, 2018 Category: Pathology Authors: Diao Y, Zhang P, Dai R, Xu J, Feng H Tags: Pathol Res Pract Source Type: research