Oral administration of a recombinant modified RBD antigen of SARS-CoV-2 as a possible immunostimulant for the care of COVID-19
Developing effective vaccines against SARS-CoV-2 that consider manufacturing limitations, equitable access, and acceptance is necessary for developing platforms to produce antigens that can be efficiently pres... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 6, 2024 Category: Microbiology Authors: Norma A. Valdez ‑Cruz, Diego Rosiles-Becerril, Constanza E. Martínez-Olivares, Enrique García‑Hernández, Laura Cobos-Marín, Daniel Garzón, Francisco E. López-Salas, Guadalupe Zavala, Axel Luviano, Alejandro Olvera, Alejandro Alagón, Octavio T. Tags: Research Source Type: research
Stable expression of HIV-1 MPER extended epitope on the surface of the recombinant probiotic bacteria Escherichia Coli Nissle 1917 using CRISPR/Cas9
Mucosal vaccines have the potential to induce protective immune responses at the sites of infection. Applying CRISPR/Cas9 editing, we aimed to develop a probiotic-based vaccine candidate expressing the HIV-1 e... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 4, 2024 Category: Microbiology Authors: Nathaniel Ninyio, Katherina Schmitt, Gladys Sergon, Charlotta Nilsson, S ören Andersson and Nikolai Scherbak Tags: Research Source Type: research
Efficient production of 2 ′-fucosyllactose from fructose through metabolically engineered recombinant Escherichia coli
The biosynthesis of human milk oligosaccharides (HMOs) using several microbial systems has garnered considerable interest for their value in pharmaceutics and food industries. 2 ′-Fucosyllactose (2′-FL), the mo... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 1, 2024 Category: Microbiology Authors: Ran You, Lei Wang, Meirong Hu and Yong Tao Tags: Research Source Type: research
Genome-wide screen identifies new set of genes for improved heterologous laccase expression in Saccharomyces cerevisiae
The yeast Saccharomyces cerevisiae is widely used as a host cell for recombinant protein production due to its fast growth, cost-effective culturing, and ability to secrete large and complex proteins. However, on... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 29, 2024 Category: Microbiology Authors: Garrett Strawn, Ryan W K Wong, Barry P Young, Michael Davey, Corey Nislow, Elizabeth Conibear, Christopher J R Loewen and Thibault Mayor Tags: Research Source Type: research
FastKnock: an efficient next-generation approach to identify all knockout strategies for strain optimization
Overproduction of desired native or nonnative biochemical(s) in (micro)organisms can be achieved through metabolic engineering. Appropriate rewiring of cell metabolism is performed by making rational changes s... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 29, 2024 Category: Microbiology Authors: Leila Hassani, Mohammad R. Moosavi, Payam Setoodeh and Habil Zare Tags: Methodology Source Type: research
Production and secretion of recombinant spider silk in Bacillus megaterium
Silk proteins have emerged as versatile biomaterials with unique chemical and physical properties, making them appealing for various applications. Among them, spider silk, known for its exceptional mechanical ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 26, 2024 Category: Microbiology Authors: Alexander Connor, R. Helen Zha and Mattheos Koffas Tags: Research Source Type: research
Identification of squalene epoxidase in triterpenes biosynthesis in Poria cocos by molecular docking and CRISPR-Cas9 gene editing
Squalene epoxidase is one of the rate-limiting enzymes in the biosynthetic pathway of membrane sterols and triterpenoids. The enzyme catalyzes the formation of oxidized squalene, which is a common precursor of... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 25, 2024 Category: Microbiology Authors: Xiao-liu Liu, Jing Xie, Zhen-ni Xie, Can Zhong, Hao Liu, Shui-han Zhang and Jian Jin Tags: Research Source Type: research
Intestinal mucosal microbiota mediate amino acid metabolism involved in the gastrointestinal adaptability to cold and humid environmental stress in mice
In this study, we explored the effects of intestinal microbiota homeostasis on the intestina... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 24, 2024 Category: Microbiology Authors: Chen-Yang Zhang, Xin-Xin Peng, Yi Wu, Mai-Jiao Peng, Tiao-Hao Liu and Zhou-Jin Tan Tags: Research Source Type: research
Engineering strategies for enhanced heterologous protein production by Saccharomyces cerevisiae
Microbial proteins are promising substitutes for animal- and plant-based proteins. S. cerevisiae, a generally recognized as safe (GRAS) microorganism, has been frequently employed to generate heterologous protein... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 22, 2024 Category: Microbiology Authors: Meirong Zhao, Jianfan Ma, Lei Zhang and Haishan Qi Tags: Review Source Type: research
Itaconate Production from Crude Substrates with U. maydis: Scale-up of an Industrially Relevant Bioprocess
Industrial by-products accrue in most agricultural or food-related production processes, but additional value chains have already been established for many of them. Crude glycerol has a 60% lower market value ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 20, 2024 Category: Microbiology Authors: Tabea Helm, Thilo Stausberg, Martina Previati, Philipp Ernst, Bianca Klein, Tobias Busche, J örn Kalinowski, Daniel Wibberg, Wolfgang Wiechert, Lien Claerhout, Nick Wierckx and Stephan Noack Tags: Research Source Type: research
Highly selective whole-cell 25-hydroxyvitamin D3 synthesis using molybdenum-dependent C25-steroid dehydrogenase and cyclodextrin recycling
The global prevalence of vitamin D (VitD) deficiency associated with numerous acute and chronic diseases has led to strategies to improve the VitD status through dietary intake of VitD-fortified foods and VitD... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 20, 2024 Category: Microbiology Authors: Dennis Kosian, Max Willistein, Ralf We ßbecher, Constantin Eggers, Oliver May and Matthias Boll Tags: Research Source Type: research
Chitinous material bioconversion by three new chitinases from the yeast Mestchnikowia pulcherrima
Chitinases are widely distributed enzymes that perform the biotransformation of chitin, one of the most abundant polysaccharides on the biosphere, into useful value-added chitooligosaccharides (COS) with a wid... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 20, 2024 Category: Microbiology Authors: Marina Minguet-Lobato, Fadia V. Cervantes, Noa M íguez, Francisco J. Plou and María Fernández-Lobato Tags: Research Source Type: research
Full quantitative resource utilization of raw mustard waste through integrating a comprehensive approach for producing hydrogen and soil amendments
Pickled mustard, the largest cultivated vegetable in China, generates substantial waste annually, leading to significant environmental pollution due to challenges in timely disposal, leading to decomposition a... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 19, 2024 Category: Microbiology Authors: Ling Zhou, JiaZhen Sun, XiaoJun Xu, MingXia Ma, YongZhi Li, Qiao Chen and HaiFeng Su Tags: Research Source Type: research
How do the carbon and nitrogen sources affect the synthesis of β-(1,3/1,6)-glucan, its structure and the susceptibility of Candida utilis yeast cells to immunolabelling with β-(1,3)-glucan monoclonal antibodies?
The need to limit antibiotic therapy due to the spreading resistance of pathogenic microorganisms to these medicinal substances stimulates research on new therapeutic agents, including the treatment and preven... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 19, 2024 Category: Microbiology Authors: Anna Bzducha-Wr óbel, Pavol Farkaš, Sandra Bieliková, Alžbeta Čížová and Marzena Sujkowska-Rybkowska Tags: Research Source Type: research
Glycylglycine promotes the solubility and antigenic utility of recombinant HCV structural proteins in a point-of-care immunoassay for detection of active viremia
Although E. coli is generally a well-opted platform for the overproduction of recombinant antigens as heterologous proteins, the optimization of expression conditions to maximize the yield of functional proteins ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 18, 2024 Category: Microbiology Authors: Heba Shawky, Ashraf A. Tabll, Reem M. Elshenawy, Naiera M. Helmy, Rehab I. Moustafa, Yasser K. Elesnawy, Marwa M. Abdelghany and Yasmine S. El-Abd Tags: Research Source Type: research
