Microbial inoculums improve growth and health of Heteropneustes fossilis via biofloc-driven aquaculture
Biofloc technology aims to maximize fish farming productivity by effectively breaking down ammonia and nitrite, promoting healthy flocculation, and enhancing the growth and immunity of cultured animals. Howeve... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 2, 2023 Category: Microbiology Authors: Vikash Kumar, Himanshu Sekhar Swain, Paton Vuong, Suvra Roy, Aurobinda Upadhyay, Ramesh Chandra Malick, Kampan Bisai, Parwinder Kaur and Basanta Kumar Das Tags: Research Source Type: research

Involvement of a flavoprotein, acetohydroxyacid synthase, in growth and riboflavin production in riboflavin-overproducing Ashbya gossypii mutant
Previously, we isolated a riboflavin-overproducing Ashbya gossypii mutant (MT strain) and discovered some mutations in genes encoding flavoproteins. Here, we analyzed the riboflavin production in the MT strain, i... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 22, 2023 Category: Microbiology Authors: Tatsuya Kato, Mai Kano, Ami Yokomori, Junya Azegami, Hesham A. El Enshasy and Enoch Y. Park Tags: Research Source Type: research

Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source
The filamentous fungus Trichoderma reesei has been used as a host organism for the production of lignocellulosic biomass-degrading enzymes. Although this microorganism has high potential for protein production, i... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 19, 2023 Category: Microbiology Authors: Toshiharu Arai, Mayumi Wada, Hiroki Nishiguchi, Yasushi Takimura and Jun Ishii Tags: Research Source Type: research

Yields and product comparison between Escherichia coli BL21 and W3110 in industrially relevant conditions: anti-c-Met scFv as a case study
In the biopharmaceutical industry, Escherichia coli is one of the preferred expression hosts for large-scale production of therapeutic proteins. Although increasing the product yield is important, product quality... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 19, 2023 Category: Microbiology Authors: Klaudia Arauzo-Aguilera, Luisa Buscajoni, Karin Koch, Gary Thompson, Colin Robinson and Matthias Berkemeyer Tags: Research Source Type: research

Improved biosynthesis of heme in Bacillus subtilis through metabolic engineering assisted fed-batch fermentation
Heme is an iron/porphyrin complex compound, widely used in the health care, food, and pharmaceutical industries. It is more advantageous and attractive to develop microbial cell factories to produce heme by fe... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 18, 2023 Category: Microbiology Authors: Shaomei Yang, Anlong Wang, Jiachang Li, Yunhang Shao, Fengjie Sun, Shucheng Li, Kai Cao, Hongliang Liu, Peng Xiong and Zhengquan Gao Tags: Research Source Type: research

Manufacture and evaluation of a HER2-positive breast cancer immunotoxin 4D5Fv-PE25
Human epidermal growth factor receptor 2 (HER2) positive breast cancer is an aggressive subtype, accounting for around 20% of all breast cancers. The development of HER2-targeted therapy has substantially impr... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 17, 2023 Category: Microbiology Authors: Yanjie Peng, Zhengli Wu, Zheng Pang, Lin Zhang, Dandan Song, Fang Liu, Yanhong Li and Tongjun Lin Tags: Research Source Type: research

Nano-metals forming bacteria in Egypt. II. Efficacy towards biomolecules, ultrastructure, growth parameters, and eco-friendly therapeutic of soft rot/blackleg genera
The nanoparticles (NPs) formed by Enterococcus thailandicus, Pseudomonas putida, Marinobacter hydrocarbonoclasticus, and P. geniculate were tested against soft rot/blackleg genera. The effects of NPs recorded on ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 17, 2023 Category: Microbiology Authors: Alia A. Shoeib, Nader A. Ashmawy, Ayman Kamal and Sahar Abd El Fatah Zaki Tags: Research Source Type: research

Production of a 135-residue long N-truncated human keratinocyte growth factor 1 in Escherichia coli
Palifermin (trade name Kepivance ®) is an amino-terminally truncated recombinant human keratinocyte growth factor 1 (KGF-1) with 140 residues that has been produced using Escherichia coli to prevent and treat oral... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 11, 2023 Category: Microbiology Authors: Young Su Kim, Hye-Jeong Lee, Gabriella Aphrodita Handoko, Jaehui Kim, Seong-Bo Kim, Minho Won, Jung-Ho Park and Jungoh Ahn Tags: Research Source Type: research

Yeast extracts from different manufacturers and supplementation of amino acids and micro elements reveal a remarkable impact on alginate production by A. vinelandii ATCC9046
In research and production, reproducibility is a key factor, to meet high quality and safety standards and maintain productivity. For microbial fermentations, complex substrates and media components are often ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 11, 2023 Category: Microbiology Authors: Sarah Sparviero, Max Daniel Dicke, Tobias M. Rosch, Tania Castillo, Holjes Salgado-Lugo, Enrique Galindo, Carlos Pe ña and Jochen Büchs Tags: Research Source Type: research

Current genetic strategies to investigate gene functions in Trichoderma reesei
The filamentous fungus Trichoderma reesei (teleomorph Hypocrea jecorina, Ascomycota) is a well-known lignocellulolytic enzymes-producing strain in industry. To increase the fermentation titer of lignocellulolytic... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 10, 2023 Category: Microbiology Authors: Chixiang Ma, Jialong Liu, Jiaxin Tang, Yuanlu Sun, Xiaojie Jiang, Tongtong Zhang, Yan Feng, Qinghua Liu and Lei Wang Tags: Review Source Type: research

Mucosal immunization with lactiplantibacillus plantarum-displaying recombinant SARS-CoV-2 epitopes on the surface induces humoral and mucosal immune responses in mice
The use of probiotic lactic acid bacteria as a mucosal vaccine vector is considered a promising alternative compared to the use of other microorganisms because of its “Generally Regarded as Safe” status, its p... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 9, 2023 Category: Microbiology Authors: In-Chan Hwang, Valerie Diane Valeriano, Ji Hoon Song, Marcela Pereira, Ju Kyoung Oh, Kyudong Han, Lars Engstrand and Dae-Kyung Kang Tags: Research Source Type: research

Preparation of a Klebsiella pneumoniae conjugate nanovaccine using glycol-engineered Escherichia coli
Engineered strains of Escherichia coli have been used to produce bioconjugate vaccines using Protein Glycan Coupling Technology (PGCT). Nanovaccines have also entered the vaccine development arena with advances i... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 6, 2023 Category: Microbiology Authors: Yan Liu, Chao Pan, Kangfeng Wang, Yan Guo, YanGe Sun, Xiang Li, Peng Sun, Jun Wu, Hengliang Wang and Li Zhu Tags: Research Source Type: research

Exploring the antimicrobial, antiviral, antioxidant, and antitumor potentials of marine Streptomyces tunisiensis W4MT573222 pigment isolated from Abu-Qir sediments, Egypt
Due to the therapeutic importance of microbial pigments, these pigments are receiving the attention of researchers. In this present study 60 isolates were isolated from sediments of Abu-Qir coast of the Medite... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 5, 2023 Category: Microbiology Authors: Walaa M. Ibrahim, Zakia A. Olama, G. M. Abou-elela, Heba S. Ramadan, Ghada E. Hegazy and Dalia El S. El Badan Tags: Research Source Type: research

High-level production of Aspergillus niger prolyl endopeptidase from agricultural residue and its application in beer brewing
Prolyl endopeptidase from Aspergillus niger (AN-PEP) is a prominent serine proteinase with various potential applications in the food and pharmaceutical industries. However, the availability of efficient and low-... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 4, 2023 Category: Microbiology Authors: Minglu Liu, Meng Hu, Hui Zhou, Zhiyang Dong and Xiuzhen Chen Tags: Research Source Type: research

Optimized enantioselective (S)-2-hydroxypropiophenone synthesis by free- and encapsulated-resting cells of Pseudomonas putida
Aromatic α-hydroxy ketones, such as S-2-hydroxypropiophenone (2-HPP), are highly valuable chiral building blocks useful for the synthesis of various pharmaceuticals and natural products. In the present study, ena... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 3, 2023 Category: Microbiology Authors: Reihaneh Kordesedehi, Mohammad Ali Asadollahi, Azar Shahpiri, Davoud Biria and Pablo Iv án Nikel Tags: Research Source Type: research