Microbial synthesis of the plant natural product precursor p-coumaric acid with Corynebacterium glutamicum
Phenylpropanoids such as p-coumaric acid represent important precursors for the synthesis of a broad range of plant secondary metabolites including stilbenoids, flavonoids, and lignans, which are of pharmacologic... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - October 13, 2023 Category: Microbiology Authors: Mario Mutz, Dominic K östers, Benedikt Wynands, Nick Wierckx and Jan Marienhagen Tags: Research Source Type: research
Development of a counterselectable system for rapid and efficient CRISPR-based genome engineering in Zymomonas mobilis
Zymomonas mobilis is an important industrial bacterium ideal for biorefinery and synthetic biology studies. High-throughput CRISPR-based genome editing technologies have been developed to enable targeted engineer... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - October 13, 2023 Category: Microbiology Authors: Yanli Zheng, Hongmei Fu, Jue Chen, Jie Li, Yuejie Bian, Ping Hu, Lei Lei, Yihan Liu, Jiangke Yang and Wenfang Peng Tags: Research Source Type: research
The transcription factor GCN4 contributes to maintaining intracellular amino acid contents under nitrogen-limiting conditions in the mushroom Ganoderma lucidum
Edible mushrooms are delicious in flavour and rich in high-quality protein and amino acids required by humans. A transcription factor, general control nonderepressible 4 (GCN4), can regulate the expression of ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - October 10, 2023 Category: Microbiology Authors: Lingdan Lian, Jinjin Qiao, Xiaoyu Guo, Zhenzhen Xing, Ang Ren, Mingwen Zhao and Jing Zhu Tags: Research Source Type: research
Metabolic flux analysis of coenzyme Q10 synthesized by Rhodobacter sphaeroides under the influence of different pH regulators
Coenzyme Q10 (CoQ10) is crucial for human beings, especially in the fields of biology and medicine. The aim of this experiment was to investigate the conditions for increasing CoQ10 production. At present, microb... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - October 10, 2023 Category: Microbiology Authors: Yujun Xiao, Yi Zheng, Yong Zhou, Chaofan Yu and Ting-E Ye Tags: Research Source Type: research
Improved production of β-carotene in light-powered Escherichia coli by co-expression of Gloeobacter rhodopsin expression
Providing sufficient and usable energy for the cell factory has long been a heated issue in biosynthesis as solar energy has never been rooted out from the strategy for improvement, and turning Escherichia coli (... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - October 10, 2023 Category: Microbiology Authors: Chao-Yu Lee, Kai-Wen Chen, Chih-Lu Chiang, Hsuan-Yu Kao, Hao-Cheng Yu, Hsiao-Ching Lee and Wen-Liang Chen Tags: Research Source Type: research
Improvement of ethanol and 2,3-butanediol production in Saccharomyces cerevisiae by ATP wasting
“ATP wasting” has been observed in 13C metabolic flux analyses of Saccharomyces cerevisiae, a yeast strain commonly used to produce ethanol. Some strains of S. cerevisiae, such as the sake strain Kyokai 7, consum... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - October 8, 2023 Category: Microbiology Authors: Futa Yatabe, Taisuke Seike, Nobuyuki Okahashi, Jun Ishii and Fumio Matsuda Tags: Research Source Type: research
Analysis of protein secretion in Bacillus subtilis by combining a secretion stress biosensor strain with an in vivo split GFP assay
Bacillus subtilis is one of the workhorses in industrial biotechnology and well known for its secretion potential. Efficient secretion of recombinant proteins still requires extensive optimization campaigns and s... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - October 7, 2023 Category: Microbiology Authors: Patrick Lenz, Patrick J. Bakkes, Carolin M üller, Marzena Malek, Roland Freudl, Marco Oldiges, Thomas Drepper, Karl-Erich Jaeger and Andreas Knapp Tags: Research Source Type: research
Statistical optimization of waste molasses-based exopolysaccharides and self-sustainable bioelectricity production for dual chamber microbial fuel cell by Bacillus piscis
The application of exopolysaccharide-producing bacteria (EPS) in dual chamber microbial fuel cells (DCMFC) is critical which can minimize the chemical oxygen demand (COD) of molasses with bioelectricity produc... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - October 6, 2023 Category: Microbiology Authors: Ebtehag A. E. Sakr, Dena Z. Khater, Zeinab M. H. Kheiralla and Kamel M. El ‑khatib Tags: Research Source Type: research
Development of engineered Candida tropicalis strain for efficient corncob-based xylitol-ethanol biorefinery
Xylitol has a wide range of applications in the pharmaceuticals, cosmetic, food and beverage industry. Microbial xylitol production reduces the risk of contamination and is considered as environment friendly a... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - October 6, 2023 Category: Microbiology Authors: Anup Kumar Singh, Farha Deeba, Mohit Kumar, Sonam Kumari, Shahid Ali Wani, Tanushree Paul and Naseem A. Gaur Tags: Research Source Type: research
Exploring the potential of Bacillus subtilis as cell factory for food ingredients and special chemicals
Bacillus subtilis has been established as model microorganism for fundamental research in the laboratory on protein production/secretion and sporulation and as model bacterium for controlling spoilage in the food... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - September 30, 2023 Category: Microbiology Authors: Taichi Chen, Stanley Brul and Jeroen Hugenholtz Tags: Research Source Type: research
Refactoring the architecture of a polyketide gene cluster enhances docosahexaenoic acid production in Yarrowia lipolytica through improved expression and genetic stability
Long-chain polyunsaturated fatty acids (LC-PUFAs), such as docosahexaenoic acid (DHA), are essential for human health and have been widely used in the food and pharmaceutical industries. However, the limited a... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - September 29, 2023 Category: Microbiology Authors: Demian Dietrich, Sofija Jovanovic-Gasovic, Peng Cao, Michael Kohlstedt and Christoph Wittmann Tags: Research Source Type: research
Economical production of Pichia pastoris single cell protein from methanol at industrial pilot scale
Methanol, synthesized from CO2, is a potentially sustainable one-carbon (C1) resource for biomanufacturing. The use of methanol as a feedstock to produce single cell protein (SCP) has been investigated for decade... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - September 28, 2023 Category: Microbiology Authors: Jiao Meng, Shufan Liu, Le Gao, Kai Hong, Shuguang Liu and Xin Wu Tags: Research Source Type: research
Cytotoxic effects of halophilic archaea metabolites on ovarian cancer cell lines
Ovarian cancer is one of the most frequent and deadly gynaecological cancers, often resistant to platinum-based chemotherapy, the current standard of care. Halophilic microorganisms have been shown to produce ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - September 28, 2023 Category: Microbiology Authors: Magdalena Kowalewicz-Kulbat, Krzysztof T. Krawczyk, Izabela Szulc-Kielbik, Sebastian Rykowski, Marta Denel-Bobrowska, Agnieszka B. Olejniczak, Camille Locht and Magdalena Klink Tags: Research Source Type: research
Synthesis of isobutanol using acetate as sole carbon source in Escherichia coli
With concerns about depletion of fossil fuel and environmental pollution, synthesis of biofuels such as isobutanol from low-cost substrate by microbial cell factories has attracted more and more attention. As ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - September 27, 2023 Category: Microbiology Authors: Pengfei Gu, Shuo Zhao, Hao Niu, Chengwei Li, Shuixing Jiang, Hao Zhou and Qiang Li Tags: Research Source Type: research
Exposure of the static magnetic fields on the microbial growth rate and the sludge properties in the complete-mix activated sludge process (a Lab-scale study)
In this study, the effect of static magnetic fields (SMFs) on improving the performance of activated sludge process to enhance the higher rate of microbial growth biomass and improve sludge settling characteri... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - September 27, 2023 Category: Microbiology Authors: Ghorban Asgari, Abdolmotaleb Seid-Mohammadi, Reza Shokoohi, Mohammad Reza Samarghandi, Glen T. Daigger, Behrooz Malekolkalami and Ramin Khoshniyat Tags: Research Source Type: research
