Carboligation of 5-(hydroxymethyl)furfural via whole-cell catalysis to form C12 furan derivatives and their use for hydrazone formation
Biobased 5-(hydroxymethyl)furfural (5-HMF) is an important platform that offers numerous possibilities for upgrading to a range of chemical, material and fuel products. One reaction of special interest is the ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 29, 2023 Category: Microbiology Authors: Sara Jonsdottir Glaser, Sang-Hyun Pyo, Nicola Rehnberg, D örte Rother and Rajni Hatti-Kaul Tags: Research Source Type: research

Characterization of 17 β-estradiol-degrading enzyme from Microbacterium sp. MZT7 and its function on E2 biodegradation in wastewater
17 β-estradiol (E2) residues exhibit harmful effects both for human and animals and have got global attention of the scientific community. Microbial enzymes are considered as one of the effective strategies hav... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 27, 2023 Category: Microbiology Authors: Peng Hao, Hanyu Pan, Zongshuo Lv, Jingyi Zhang, Lixia Wang, Yanbin Zhu, Wangdui Basang and Yunhang Gao Tags: Research Source Type: research

Metabolic engineering of Escherichia coli for high-level production of violaxanthin
Xanthophylls are a large class of carotenoids that are found in a variety of organisms and play particularly important roles in the light-harvesting and photoprotection processes of plants and algae. Violaxant... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 21, 2023 Category: Microbiology Authors: Dong Xinrui, Liu Bo, Bao Yihong, Liu Weifeng and Tao Yong Tags: Research Source Type: research

Insight into broad substrate specificity and synergistic contribution of a fungal α-glucosidase in Chinese Nong-flavor daqu
Chinese Nong-favor daqu, the presentative liquor starter of Baijiu, has been enriched with huge amounts of enzymes in degrading various biological macromolecules by openly man-made process for thousand years. ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 15, 2023 Category: Microbiology Authors: Zhuolin Yi, Lanchai Chen, Yanling Jin, Yi Shen, Nian Liu, Yang Fang, Yao Xiao, Xi Wang, Kui Peng, Kaize He and Hai Zhao Tags: Research Source Type: research

Engineering of cyclodextrin glycosyltransferase improves the conversion efficiency of rebaudioside A to glucosylated steviol glycosides and increases the content of short-chain glycosylated steviol glycoside
Compared with steviol glycosides, the taste of glucosylated steviol glycosides is better and more similar to that of sucrose. At present, cyclodextrin glucanotransferase (CGTase) is primarily used to catalyze ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 14, 2023 Category: Microbiology Authors: Ruiqin Zhang, Ruiqi Tang, Wei Wang, Jiahua Bi, Xianrui Xu, Qiuling Fan, Yanjun Li and Qihe Chen Tags: Research Source Type: research

Establishment of CRISPR-Cas9 system in Bifidobacteria animalis AR668
Bifidobacteria are representative intestinal probiotics that have extremely high application value in the food and medical fields. However, the lack of molecular biology tools limits the research on functional ge... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 12, 2023 Category: Microbiology Authors: Jiao Li, Xin Song, Zhiqiang Xiong, Guangqiang Wang, Yongjun Xia, Yijin Yang and Lianzhong Ai Tags: Research Source Type: research

Lactiplantibacillus plantarum: a new example of inclusion body producing bacteria
Lactic Acid Bacteria such as Lactococcus lactis, Latilactobacillus sakei (basonym: Lactobacillus sakei) and Lactiplantibacillus plantarum (basonym: Lactobacillus plantarum) have gained importance as recombinant c... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 9, 2023 Category: Microbiology Authors: Ricardo Balt à-Foix, Caterina Serrano-Adrover, Adrià López-Cano, Laia Gifre-Renom, Alejandro Sanchez-Chardi, Anna Arís and Elena Garcia-Fruitós Tags: Research Source Type: research

Pseudomonas aeruginosa ’s greenish-blue pigment pyocyanin: its production and biological activities
A subject of great interest is the bioprospecting of microorganisms and their bioactive byproducts, such as pigments. Microbial pigments have various benefits, including being safe to use due to their natural ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 8, 2023 Category: Microbiology Authors: Ahmed A. Abdelaziz, Amal M. Abo Kamer, Khaled B. Al-Monofy and Lamiaa A. Al-Madboly Tags: Review Source Type: research

Lipid production and cellular changes in Fremyella diplosiphon exposed to nanoscale zerovalent iron nanoparticles and ampicillin
With the dramatic decrease in fossil fuel stocks and their detrimental effects on the environment, renewable energy sources have gained imminent importance in the mitigation of emissions. As lipid-enriched ene... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 7, 2023 Category: Microbiology Authors: Yavuz S. Yalcin, Busra Aydin, Huan Chen, Samson Gichuki and Viji Sitther Tags: Research Source Type: research

Improving therapeutic protein secretion in the probiotic yeast Saccharomyces boulardii using a multifactorial engineering approach
The probiotic yeast Saccharomyces boulardii (Sb) is a promising chassis to deliver therapeutic proteins to the gut due to Sb ’s innate therapeutic properties, resistance to phage and antibiotics, and high protein ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 7, 2023 Category: Microbiology Authors: Deniz Durmusoglu, Ibrahim Al ’Abri, Zidan Li, Taufika Islam Williams, Leonard B. Collins, José L. Martínez and Nathan Crook Tags: Research Source Type: research

Bioactive compounds and biomedical applications of endophytic fungi: a recent review
Human life has been significantly impacted by the creation and spread of novel species of antibiotic-resistant bacteria and virus strains that are difficult to manage. Scientists and researchers have recently ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 6, 2023 Category: Microbiology Authors: Amr H. Hashem, Mohamed S. Attia, Esalm K. Kandil, Mahmoud M. Fawzi, Ahmed S. Abdelrahman, Mohamed S. Khader, Mohamed A. Khodaira, Abdallah E. Emam, Mohamed A. Goma and Amer M. Abdelaziz Tags: Review Source Type: research

Microbial inoculums improve growth and health of Heteropneustes fossilis via biofloc-driven aquaculture
Biofloc technology aims to maximize fish farming productivity by effectively breaking down ammonia and nitrite, promoting healthy flocculation, and enhancing the growth and immunity of cultured animals. Howeve... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - June 2, 2023 Category: Microbiology Authors: Vikash Kumar, Himanshu Sekhar Swain, Paton Vuong, Suvra Roy, Aurobinda Upadhyay, Ramesh Chandra Malick, Kampan Bisai, Parwinder Kaur and Basanta Kumar Das Tags: Research Source Type: research

Involvement of a flavoprotein, acetohydroxyacid synthase, in growth and riboflavin production in riboflavin-overproducing Ashbya gossypii mutant
Previously, we isolated a riboflavin-overproducing Ashbya gossypii mutant (MT strain) and discovered some mutations in genes encoding flavoproteins. Here, we analyzed the riboflavin production in the MT strain, i... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 22, 2023 Category: Microbiology Authors: Tatsuya Kato, Mai Kano, Ami Yokomori, Junya Azegami, Hesham A. El Enshasy and Enoch Y. Park Tags: Research Source Type: research

Inducer-free recombinant protein production in Trichoderma reesei: secretory production of endogenous enzymes and heterologous nanobodies using glucose as the sole carbon source
The filamentous fungus Trichoderma reesei has been used as a host organism for the production of lignocellulosic biomass-degrading enzymes. Although this microorganism has high potential for protein production, i... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 19, 2023 Category: Microbiology Authors: Toshiharu Arai, Mayumi Wada, Hiroki Nishiguchi, Yasushi Takimura and Jun Ishii Tags: Research Source Type: research

Yields and product comparison between Escherichia coli BL21 and W3110 in industrially relevant conditions: anti-c-Met scFv as a case study
In the biopharmaceutical industry, Escherichia coli is one of the preferred expression hosts for large-scale production of therapeutic proteins. Although increasing the product yield is important, product quality... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - May 19, 2023 Category: Microbiology Authors: Klaudia Arauzo-Aguilera, Luisa Buscajoni, Karin Koch, Gary Thompson, Colin Robinson and Matthias Berkemeyer Tags: Research Source Type: research