Development and characterization of a novel nanobody with SRMV neutralizing activity
Peste des petits ruminants (PPR) is an acute, contact infectious disease caused by the small ruminant morbillivirus (SRMV), and its morbidity in goats and sheep can be up to 100% with significant mortality. Na... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 10, 2024 Category: Microbiology Authors: Miao Sun, Changjiang Wang, Huaye Luo, Yanfei Chen, Guanggang Qu, Jian Chen, Ling Li, Min Zhang and Qinghong Xue Tags: Research Source Type: research
Correction: Systematic engineering enables efficient biosynthesis of L-phenylalanine in E. Coli from inexpensive aromatic precursors
(Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 10, 2024 Category: Microbiology Authors: Mengzhen Nie, Jingyu Wang, Zeyao Chen, Chenkai Cao and Kechun Zhang Tags: Correction Source Type: research
Correction: A novel strategy for L-arginine production in engineered Escherichia coli
(Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 10, 2024 Category: Microbiology Authors: Mengzhen Nie, Jingyu Wang and Kechun Zhang Tags: Correction Source Type: research
Quantifying microbial robustness in dynamic environments using microfluidic single-cell cultivation
Microorganisms must respond to changes in their environment. Analysing the robustness of functions (i.e. performance stability) to such dynamic perturbations is of great interest in both laboratory and industr... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 9, 2024 Category: Microbiology Authors: Luisa Bl öbaum, Luca Torello Pianale, Lisbeth Olsson and Alexander Grünberger Tags: Methodology Source Type: research
Protein production dynamics and physiological adaptation of recombinant Komagataella phaffii at near-zero growth rates
Specific productivity (qP) in yeast correlates with growth, typically peaking at intermediate or maximum specific growth rates ( μ). Understanding the factors limiting productivity at extremely low μ might reveal ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 8, 2024 Category: Microbiology Authors: Corinna Rebnegger, Benjamin L. Coltman, Viktoria Kowarz, David A. Pe ña, Axel Mentler, Christina Troyer, Stephan Hann, Harald Schöny, Gunda Koellensperger, Diethard Mattanovich and Brigitte Gasser Tags: Research Source Type: research
Discovery of a high-performance phage-derived promoter/repressor system for probiotic lactobacillus engineering
The Lactobacillaceae family comprises many species of great importance for the food and healthcare industries, with numerous strains identified as beneficial for humans and used as probiotics. Hence, there is a g... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 7, 2024 Category: Microbiology Authors: Marc Blanch-Asensio, Varun Sai Tadimarri, Alina Wilk and Shrikrishnan Sankaran Tags: Research Source Type: research
Construction of recombinant Lactococcus expressing thymosin and interferon fusion protein and its application as an immune adjuvant
In recent years, biosafety and green food safety standards have increased the demand for immune enhancers and adjuvants. In the present study, recombinant food-grade Lactococcus lactis (r-L. lactis-T α1-IFN) expre... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 6, 2024 Category: Microbiology Authors: Zengqi Liu, Suhua Zhang, Hongjiao Hu, He Wang, Yu Qiu, Mingqi Dong, Muping Wang, Ziyang Cui, Hongyu Cui, Yunfeng Wang and Gaoming He Tags: Research Source Type: research
Oral administration of a recombinant modified RBD antigen of SARS-CoV-2 as a possible immunostimulant for the care of COVID-19
Developing effective vaccines against SARS-CoV-2 that consider manufacturing limitations, equitable access, and acceptance is necessary for developing platforms to produce antigens that can be efficiently pres... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 6, 2024 Category: Microbiology Authors: Norma A. Valdez ‑Cruz, Diego Rosiles-Becerril, Constanza E. Martínez-Olivares, Enrique García‑Hernández, Laura Cobos-Marín, Daniel Garzón, Francisco E. López-Salas, Guadalupe Zavala, Axel Luviano, Alejandro Olvera, Alejandro Alagón, Octavio T. Tags: Research Source Type: research
Stable expression of HIV-1 MPER extended epitope on the surface of the recombinant probiotic bacteria Escherichia Coli Nissle 1917 using CRISPR/Cas9
Mucosal vaccines have the potential to induce protective immune responses at the sites of infection. Applying CRISPR/Cas9 editing, we aimed to develop a probiotic-based vaccine candidate expressing the HIV-1 e... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 4, 2024 Category: Microbiology Authors: Nathaniel Ninyio, Katherina Schmitt, Gladys Sergon, Charlotta Nilsson, S ören Andersson and Nikolai Scherbak Tags: Research Source Type: research
Efficient production of 2 ′-fucosyllactose from fructose through metabolically engineered recombinant Escherichia coli
The biosynthesis of human milk oligosaccharides (HMOs) using several microbial systems has garnered considerable interest for their value in pharmaceutics and food industries. 2 ′-Fucosyllactose (2′-FL), the mo... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - February 1, 2024 Category: Microbiology Authors: Ran You, Lei Wang, Meirong Hu and Yong Tao Tags: Research Source Type: research
Genome-wide screen identifies new set of genes for improved heterologous laccase expression in Saccharomyces cerevisiae
The yeast Saccharomyces cerevisiae is widely used as a host cell for recombinant protein production due to its fast growth, cost-effective culturing, and ability to secrete large and complex proteins. However, on... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 29, 2024 Category: Microbiology Authors: Garrett Strawn, Ryan W K Wong, Barry P Young, Michael Davey, Corey Nislow, Elizabeth Conibear, Christopher J R Loewen and Thibault Mayor Tags: Research Source Type: research
FastKnock: an efficient next-generation approach to identify all knockout strategies for strain optimization
Overproduction of desired native or nonnative biochemical(s) in (micro)organisms can be achieved through metabolic engineering. Appropriate rewiring of cell metabolism is performed by making rational changes s... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 29, 2024 Category: Microbiology Authors: Leila Hassani, Mohammad R. Moosavi, Payam Setoodeh and Habil Zare Tags: Methodology Source Type: research
Production and secretion of recombinant spider silk in Bacillus megaterium
Silk proteins have emerged as versatile biomaterials with unique chemical and physical properties, making them appealing for various applications. Among them, spider silk, known for its exceptional mechanical ... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 26, 2024 Category: Microbiology Authors: Alexander Connor, R. Helen Zha and Mattheos Koffas Tags: Research Source Type: research
Identification of squalene epoxidase in triterpenes biosynthesis in Poria cocos by molecular docking and CRISPR-Cas9 gene editing
Squalene epoxidase is one of the rate-limiting enzymes in the biosynthetic pathway of membrane sterols and triterpenoids. The enzyme catalyzes the formation of oxidized squalene, which is a common precursor of... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 25, 2024 Category: Microbiology Authors: Xiao-liu Liu, Jing Xie, Zhen-ni Xie, Can Zhong, Hao Liu, Shui-han Zhang and Jian Jin Tags: Research Source Type: research
Intestinal mucosal microbiota mediate amino acid metabolism involved in the gastrointestinal adaptability to cold and humid environmental stress in mice
In this study, we explored the effects of intestinal microbiota homeostasis on the intestina... (Source: Microbial Cell Factories)
Source: Microbial Cell Factories - January 24, 2024 Category: Microbiology Authors: Chen-Yang Zhang, Xin-Xin Peng, Yi Wu, Mai-Jiao Peng, Tiao-Hao Liu and Zhou-Jin Tan Tags: Research Source Type: research
