Integrative proteome and metabolome analyses reveal molecular basis underlying growth and nutrient composition in the Pacific oyster, Crassostrea gigas
In this study, two C. gigas strains with contrasted phenotypes in growth and shell color provided an ideal model for unraveling the molecular basis of growth and nutrient composition through a comparison of the proteome and metabolome. Since proteins and metabolites are the critical undertakers and the end products of cellular regulatory processes, identifying the differentially expressed proteins and metabolites would allow for discovering biomarkers and pathways that were implicated in cell growth, proliferation, and other critical functions. This work provides valuable resources in assistance with molecular breeding of ...
Source: Journal of Proteomics - October 14, 2023 Category: Biochemistry Authors: Ying Tan Yongjing Li Liting Ren Huiru Fu Qi Li Shikai Liu Source Type: research
Uterine secretome: What do the proteins say about maternal-fetal communication in buffaloes?
J Proteomics. 2023 Oct 12:105023. doi: 10.1016/j.jprot.2023.105023. Online ahead of print.ABSTRACTThe aim was to compare the UF proteomics of pregnant and non-pregnant buffalo during early pregnancy. Forty-four females were submitted to hormonal estrus synchronization and randomly divided into two groups: pregnant (n = 30) and non-pregnant (n = 14). The pregnant group was artificially inseminated and divided into a further two groups: P12 (n = 15) and P18 (n = 15). Conceptus and uterine fluid samples were collected during slaughter at, respectively, 12 and 18 days after insemination. Of all the inseminated females, only ei...
Source: Journal of Proteomics - October 14, 2023 Category: Biochemistry Authors: Viviane Maria Codognoto Fabiana Ferreira de Souza Thais Regiani Cataldi Carlos Alberto Labate La íza Sartori de Camargo Caroline Scott Roberto Rodrigues da Rosa Filho N élcio Antonio Tonizza de Carvalho Eunice Oba Source Type: research
Proteomic profile of Cryptococcus gattii biofilm: Metabolic shift and the potential activation of electron chain transport
This study investigated the comparative proteome of C. gattii R265 (VGIIa) grown under planktonic and biofilm conditions. A brief comparison with C. neoformans H99 biofilm and the use of different culture medium and surface were also evaluated. Using Multidimensional Protein Identification Technology (MudPIT), 1819 proteins were identified for both conditions, where 150 (8.2%) were considered differentially regulated (up- or down-regulated and unique in biofilm cells). Overall, the proteomic approach suggests that C. gattii R265 biofilm cells are maintained by the induction of electron transport chain for reoxidation, and ...
Source: Journal of Proteomics - October 14, 2023 Category: Biochemistry Authors: Luc élia Santi Markus Berger Jorge A Guimar ães Yohana Porto Calegari Alves Marilene H Vainstein John R Yates Walter O Beys-da-Silva Source Type: research
Integrative proteome and metabolome analyses reveal molecular basis underlying growth and nutrient composition in the Pacific oyster, Crassostrea gigas
In this study, two C. gigas strains with contrasted phenotypes in growth and shell color provided an ideal model for unraveling the molecular basis of growth and nutrient composition through a comparison of the proteome and metabolome. Since proteins and metabolites are the critical undertakers and the end products of cellular regulatory processes, identifying the differentially expressed proteins and metabolites would allow for discovering biomarkers and pathways that were implicated in cell growth, proliferation, and other critical functions. This work provides valuable resources in assistance with molecular breeding of ...
Source: Journal of Proteomics - October 14, 2023 Category: Biochemistry Authors: Ying Tan Yongjing Li Liting Ren Huiru Fu Qi Li Shikai Liu Source Type: research
Uterine secretome: What do the proteins say about maternal-fetal communication in buffaloes?
J Proteomics. 2023 Oct 12:105023. doi: 10.1016/j.jprot.2023.105023. Online ahead of print.ABSTRACTThe aim was to compare the UF proteomics of pregnant and non-pregnant buffalo during early pregnancy. Forty-four females were submitted to hormonal estrus synchronization and randomly divided into two groups: pregnant (n = 30) and non-pregnant (n = 14). The pregnant group was artificially inseminated and divided into a further two groups: P12 (n = 15) and P18 (n = 15). Conceptus and uterine fluid samples were collected during slaughter at, respectively, 12 and 18 days after insemination. Of all the inseminated females, only ei...
Source: Journal of Proteomics - October 14, 2023 Category: Biochemistry Authors: Viviane Maria Codognoto Fabiana Ferreira de Souza Thais Regiani Cataldi Carlos Alberto Labate La íza Sartori de Camargo Caroline Scott Roberto Rodrigues da Rosa Filho N élcio Antonio Tonizza de Carvalho Eunice Oba Source Type: research
Proteomic profile of Cryptococcus gattii biofilm: Metabolic shift and the potential activation of electron chain transport
This study investigated the comparative proteome of C. gattii R265 (VGIIa) grown under planktonic and biofilm conditions. A brief comparison with C. neoformans H99 biofilm and the use of different culture medium and surface were also evaluated. Using Multidimensional Protein Identification Technology (MudPIT), 1819 proteins were identified for both conditions, where 150 (8.2%) were considered differentially regulated (up- or down-regulated and unique in biofilm cells). Overall, the proteomic approach suggests that C. gattii R265 biofilm cells are maintained by the induction of electron transport chain for reoxidation, and ...
Source: Journal of Proteomics - October 14, 2023 Category: Biochemistry Authors: Luc élia Santi Markus Berger Jorge A Guimar ães Yohana Porto Calegari Alves Marilene H Vainstein John R Yates Walter O Beys-da-Silva Source Type: research
Integrative proteome and metabolome analyses reveal molecular basis underlying growth and nutrient composition in the Pacific oyster, Crassostrea gigas
In this study, two C. gigas strains with contrasted phenotypes in growth and shell color provided an ideal model for unraveling the molecular basis of growth and nutrient composition through a comparison of the proteome and metabolome. Since proteins and metabolites are the critical undertakers and the end products of cellular regulatory processes, identifying the differentially expressed proteins and metabolites would allow for discovering biomarkers and pathways that were implicated in cell growth, proliferation, and other critical functions. This work provides valuable resources in assistance with molecular breeding of ...
Source: Journal of Proteomics - October 14, 2023 Category: Biochemistry Authors: Ying Tan Yongjing Li Liting Ren Huiru Fu Qi Li Shikai Liu Source Type: research
Uterine secretome: What do the proteins say about maternal-fetal communication in buffaloes?
J Proteomics. 2023 Oct 12:105023. doi: 10.1016/j.jprot.2023.105023. Online ahead of print.ABSTRACTThe aim was to compare the UF proteomics of pregnant and non-pregnant buffalo during early pregnancy. Forty-four females were submitted to hormonal estrus synchronization and randomly divided into two groups: pregnant (n = 30) and non-pregnant (n = 14). The pregnant group was artificially inseminated and divided into a further two groups: P12 (n = 15) and P18 (n = 15). Conceptus and uterine fluid samples were collected during slaughter at, respectively, 12 and 18 days after insemination. Of all the inseminated females, only ei...
Source: Journal of Proteomics - October 14, 2023 Category: Biochemistry Authors: Viviane Maria Codognoto Fabiana Ferreira de Souza Thais Regiani Cataldi Carlos Alberto Labate La íza Sartori de Camargo Caroline Scott Roberto Rodrigues da Rosa Filho N élcio Antonio Tonizza de Carvalho Eunice Oba Source Type: research
Proteomic profile of Cryptococcus gattii biofilm: Metabolic shift and the potential activation of electron chain transport
This study investigated the comparative proteome of C. gattii R265 (VGIIa) grown under planktonic and biofilm conditions. A brief comparison with C. neoformans H99 biofilm and the use of different culture medium and surface were also evaluated. Using Multidimensional Protein Identification Technology (MudPIT), 1819 proteins were identified for both conditions, where 150 (8.2%) were considered differentially regulated (up- or down-regulated and unique in biofilm cells). Overall, the proteomic approach suggests that C. gattii R265 biofilm cells are maintained by the induction of electron transport chain for reoxidation, and ...
Source: Journal of Proteomics - October 14, 2023 Category: Biochemistry Authors: Luc élia Santi Markus Berger Jorge A Guimar ães Yohana Porto Calegari Alves Marilene H Vainstein John R Yates Walter O Beys-da-Silva Source Type: research
Integrative proteome and metabolome analyses reveal molecular basis underlying growth and nutrient composition in the Pacific oyster, Crassostrea gigas
In this study, two C. gigas strains with contrasted phenotypes in growth and shell color provided an ideal model for unraveling the molecular basis of growth and nutrient composition through a comparison of the proteome and metabolome. Since proteins and metabolites are the critical undertakers and the end products of cellular regulatory processes, identifying the differentially expressed proteins and metabolites would allow for discovering biomarkers and pathways that were implicated in cell growth, proliferation, and other critical functions. This work provides valuable resources in assistance with molecular breeding of ...
Source: Journal of Proteomics - October 14, 2023 Category: Biochemistry Authors: Ying Tan Yongjing Li Liting Ren Huiru Fu Qi Li Shikai Liu Source Type: research
Proteomics and phosphoproteomics reveal the different drought-responsive mechanisms of priming with (Z)-3-hexenyl acetate in two tea cultivars
This study investigated the physiological, proteomic, and phosphoproteomic profiling of two tea plant varieties of Longjingchangye (LJCY) and Zhongcha 108 (ZC108) with contrasting drought tolerance characteristics under drought stress. Physiological data showed that spraying Z-3-HAC exhibited higher activities of superoxide dismutase (SOD) and catalase (CAT) in both LJCY and ZC108 but lower content of malondialdehyde (MDA) in LJCY under drought stress. The proteomic and phosphoproteomic analysis suggested that the drought tolerance mechanism of Z-3-HAC in LJCY and ZC108 was different. Proteomic analyses revealed that Z-3-H...
Source: Journal of Proteomics - October 5, 2023 Category: Biochemistry Authors: Shuangshuang Wang Honglian Gu Sizhou Chen Yuchen Li Jiazhi Shen Yu Wang Zhaotang Ding Source Type: research
Proteomics and phosphoproteomics reveal the different drought-responsive mechanisms of priming with (Z)-3-hexenyl acetate in two tea cultivars
This study investigated the physiological, proteomic, and phosphoproteomic profiling of two tea plant varieties of Longjingchangye (LJCY) and Zhongcha 108 (ZC108) with contrasting drought tolerance characteristics under drought stress. Physiological data showed that spraying Z-3-HAC exhibited higher activities of superoxide dismutase (SOD) and catalase (CAT) in both LJCY and ZC108 but lower content of malondialdehyde (MDA) in LJCY under drought stress. The proteomic and phosphoproteomic analysis suggested that the drought tolerance mechanism of Z-3-HAC in LJCY and ZC108 was different. Proteomic analyses revealed that Z-3-H...
Source: Journal of Proteomics - October 5, 2023 Category: Biochemistry Authors: Shuangshuang Wang Honglian Gu Sizhou Chen Yuchen Li Jiazhi Shen Yu Wang Zhaotang Ding Source Type: research
Proteomics and phosphoproteomics reveal the different drought-responsive mechanisms of priming with (Z)-3-hexenyl acetate in two tea cultivars
This study investigated the physiological, proteomic, and phosphoproteomic profiling of two tea plant varieties of Longjingchangye (LJCY) and Zhongcha 108 (ZC108) with contrasting drought tolerance characteristics under drought stress. Physiological data showed that spraying Z-3-HAC exhibited higher activities of superoxide dismutase (SOD) and catalase (CAT) in both LJCY and ZC108 but lower content of malondialdehyde (MDA) in LJCY under drought stress. The proteomic and phosphoproteomic analysis suggested that the drought tolerance mechanism of Z-3-HAC in LJCY and ZC108 was different. Proteomic analyses revealed that Z-3-H...
Source: Journal of Proteomics - October 5, 2023 Category: Biochemistry Authors: Shuangshuang Wang Honglian Gu Sizhou Chen Yuchen Li Jiazhi Shen Yu Wang Zhaotang Ding Source Type: research
Proteomics and phosphoproteomics reveal the different drought-responsive mechanisms of priming with (Z)-3-hexenyl acetate in two tea cultivars
This study investigated the physiological, proteomic, and phosphoproteomic profiling of two tea plant varieties of Longjingchangye (LJCY) and Zhongcha 108 (ZC108) with contrasting drought tolerance characteristics under drought stress. Physiological data showed that spraying Z-3-HAC exhibited higher activities of superoxide dismutase (SOD) and catalase (CAT) in both LJCY and ZC108 but lower content of malondialdehyde (MDA) in LJCY under drought stress. The proteomic and phosphoproteomic analysis suggested that the drought tolerance mechanism of Z-3-HAC in LJCY and ZC108 was different. Proteomic analyses revealed that Z-3-H...
Source: Journal of Proteomics - October 5, 2023 Category: Biochemistry Authors: Shuangshuang Wang Honglian Gu Sizhou Chen Yuchen Li Jiazhi Shen Yu Wang Zhaotang Ding Source Type: research
Proteomics and phosphoproteomics reveal the different drought-responsive mechanisms of priming with (Z)-3-hexenyl acetate in two tea cultivars
This study investigated the physiological, proteomic, and phosphoproteomic profiling of two tea plant varieties of Longjingchangye (LJCY) and Zhongcha 108 (ZC108) with contrasting drought tolerance characteristics under drought stress. Physiological data showed that spraying Z-3-HAC exhibited higher activities of superoxide dismutase (SOD) and catalase (CAT) in both LJCY and ZC108 but lower content of malondialdehyde (MDA) in LJCY under drought stress. The proteomic and phosphoproteomic analysis suggested that the drought tolerance mechanism of Z-3-HAC in LJCY and ZC108 was different. Proteomic analyses revealed that Z-3-H...
Source: Journal of Proteomics - October 5, 2023 Category: Biochemistry Authors: Shuangshuang Wang Honglian Gu Sizhou Chen Yuchen Li Jiazhi Shen Yu Wang Zhaotang Ding Source Type: research
