Global proteome of the saprophytic strain Leptospira biflexa and comparative analysis with pathogenic strain Leptospira interrogans uncover new pathogenesis mechanisms
J Proteomics. 2024 Feb 14:105125. doi: 10.1016/j.jprot.2024.105125. Online ahead of print.ABSTRACTLeptospira is a genus of bacteria that includes free-living saprophytic species found in water or soil, and pathogenic species, which are the etiologic agents of leptospirosis. Besides all the efforts, there are only a few proteins described as virulence factors in the pathogenic strain L. interrogans. This work aims to perform L. biflexa serovar Patoc1 strain Paris global proteome and to compare with the proteome database of pathogenic L. interrogans serovar Copenhageni strain Fiocruz L1-130. We identified a total of 2327 exp...
Source: Journal of Proteomics - February 16, 2024 Category: Biochemistry Authors: Edson G Nascimento Filho M ônica L Vieira Meriellen Dias Maria A Mendes Fabio B Sanchez Jo ão C Setubal Marcos B Heinemann Gisele O Souza Daniel C Pimenta Ana L T O Nascimento Source Type: research
Quantitative proteomic and metabolomic profiling reveals different osmoregulation mechanisms of tilapia cells coping with different hyperosmotic stress
This study aimed to investigate the different regulatory mechanisms of euryhaline fish under regular hyperosmotic and extreme hyperosmotic stress. The OmB (Oreochromis mossambicus brain) cells were exposed to three treatments: control, regular hyperosmotic stress and extreme hyperosmotic stress. After 12 h exposure, proteomics, metabolomics analyses and integrative analyses were explored. Both kinds of stress lead to lowering cell growth and morphology changes, while under regular hyperosmotic stress, the up-regulated processes related with compatible organic osmolytes synthesis are crucial strategy for the euryhaline fish...
Source: Journal of Proteomics - February 12, 2024 Category: Biochemistry Authors: Jingyu Pan Minxu Wang Jiahua Zhu Yuxing Huang Fan Zhang Erchao Li Jianguang Qin Liqiao Chen Xiaodan Wang Source Type: research
Quantitative proteomic and metabolomic profiling reveals different osmoregulation mechanisms of tilapia cells coping with different hyperosmotic stress
This study aimed to investigate the different regulatory mechanisms of euryhaline fish under regular hyperosmotic and extreme hyperosmotic stress. The OmB (Oreochromis mossambicus brain) cells were exposed to three treatments: control, regular hyperosmotic stress and extreme hyperosmotic stress. After 12 h exposure, proteomics, metabolomics analyses and integrative analyses were explored. Both kinds of stress lead to lowering cell growth and morphology changes, while under regular hyperosmotic stress, the up-regulated processes related with compatible organic osmolytes synthesis are crucial strategy for the euryhaline fish...
Source: Journal of Proteomics - February 12, 2024 Category: Biochemistry Authors: Jingyu Pan Minxu Wang Jiahua Zhu Yuxing Huang Fan Zhang Erchao Li Jianguang Qin Liqiao Chen Xiaodan Wang Source Type: research
Oxidation and cross-link of tyrosine-rich proteins are involved in the periostracum formation of the green mussel Perna viridis (Linnaeus)
This study provides some insights into the first step of mussel shell formation and the robust adaptation of P. viridis to diverse habitats. These findings also help to reveal the potential acclimation of bivalves to the projected acidifying seawater.PMID:38331166 | DOI:10.1016/j.jprot.2024.105112 (Source: Journal of Proteomics)
Source: Journal of Proteomics - February 8, 2024 Category: Biochemistry Authors: Jingliang Huang Feng Lin Chuang Liu Maoguo Luo Source Type: research
Proteomic and metabolomic analysis of the serum of patients with tick-borne encephalitis
In this study, a comprehensive analysis was conducted to characterize the serum metabolome and proteome of adult patients infected with TBEV, in comparison to a control group of healthy individuals. Liquid chromatography tandem mass spectrometry (LC-MS) was employed to monitor metabolic and proteomic alternations throughout the progression of the disease, significant physiological changes associated with different stages of the disease were identified. A total of 44 proteins and 115 metabolites exhibited significantly alternations in the sera of patients diagnosed with TBE. Gene Ontology and Kyoto Encyclopedia of Genes and...
Source: Journal of Proteomics - February 8, 2024 Category: Biochemistry Authors: YanDan Du LePing Ou HaiJun Zheng DeSheng Lu YiQing Niu ChunXi Bao Meng Zhang ZhiHui Mi Source Type: research
Effect of feeding fermented distiller's grains diets on immune status and metabolomics of spleen and mesenteric lymph nodes in finishing cattle
This study extends our understanding of the metabolomics of the spleen and MLN in FDG and helps to further understand of the immunomodulatory effects of the FDG diet.PMID:38325729 | DOI:10.1016/j.jprot.2024.105107 (Source: Journal of Proteomics)
Source: Journal of Proteomics - February 7, 2024 Category: Biochemistry Authors: Shihui Mei Guangxia He Tiantian Zhang Ze Chen Rong Zhang Yixiao Liao Mingming Zhu Duhan Xu Yanjuan Shen Bijun Zhou Kaigong Wang Chunmei Wang Chao Chen Erpeng Zhu Zhentao Cheng Source Type: research
Technical report: The clinically useful selection of proteins protocol: An approach to identify clinically useful proteins for validation
J Proteomics. 2024 Feb 5;296:105110. doi: 10.1016/j.jprot.2024.105110. Online ahead of print.ABSTRACTClinical proteomics studies aiming to develop markers of clinical outcome or disease typically involve distinct discovery and validation stages, neither of which focus on the clinical applicability of the candidate markers studied. Our clinically useful selection of proteins (CUSP) protocol proposes a rational approach, with statistical and non-statistical components, to identify proteins for the validation phase of studies that could be most effective markers of disease or clinical outcome. Additionally, this protocol cons...
Source: Journal of Proteomics - February 7, 2024 Category: Biochemistry Authors: Ella E K Swaney Stephen Hearps Paul Monagle Michael H A Roehrl Vera Ignjatovic Source Type: research
Development of Mass Spectrometry Imaging on skeletal muscle to characterize the local pro-inflammatory and pro-resolution lipid responses in a vaccination context
J Proteomics. 2024 Feb 5;296:105105. doi: 10.1016/j.jprot.2024.105105. Online ahead of print.ABSTRACTVaccine reactogenicity is well documented at the clinical level but the mechanism involved at the local or systemic level are still poorly understood. Muscular tissue where most vaccines are administered is the first place of interaction between the vaccine formulation and the host's immune cells. So far, this site of vaccine administration is not well documented from a mechanistic standpoint. The study of early molecular events at the injection site is crucial to understand the local response to vaccines. In this paper, we...
Source: Journal of Proteomics - February 7, 2024 Category: Biochemistry Authors: Thomas Ribette Yannick Charretier Sebastien Laurent Patrick Syntin Emilie Chautard Xavier Meniche Marion Darnaud Fr édéric Bequet Laurent Beloeil Fabienne Piras-Douce Josephine Abi-Ghanem Source Type: research
Optimal conditions for carrying out trypsin digestions on complex proteomes: From bulk samples to single cells
J Proteomics. 2024 Feb 5:105109. doi: 10.1016/j.jprot.2024.105109. Online ahead of print.ABSTRACTTo identify proteins by the bottom-up mass spectrometry workflow, enzymatic digestion is essential to break down proteins into smaller peptides amenable to both chromatographic separation and mass spectrometric analysis. Trypsin is the most extensively used protease due to its high cleavage specificity and generation of peptides with desirable positively charged N-and C-terminal amino acid residues that are amenable to reverse phase HPLC separation and MS/MS analyses. However, trypsin can yield variable digestion profiles and i...
Source: Journal of Proteomics - February 7, 2024 Category: Biochemistry Authors: M Shahid Mansuri Shveta Bathla TuKiet T Lam Angus C Nairn Kenneth R Williams Source Type: research
Proteomic profiles of Leptospira borgpetersenii serovar Hardjo strains JB197 and HB203 cultured at different temperatures
In this study, proteomic profiles of strains JB197 and HB203 were analyzed, and results revealed diverse protein expression profiles of outer membrane proteins, as well as proteins functioning in motility and growth.PMID:38320623 | DOI:10.1016/j.jprot.2024.105106 (Source: Journal of Proteomics)
Source: Journal of Proteomics - February 6, 2024 Category: Biochemistry Authors: Ellie J Putz Luis G V Fernandes Kaitlyn M Sarlo Davila Julian Whitelegge John D Lippolis Jarlath E Nally Source Type: research
Proteomic insight towards key modulating proteins regulated by the aryl hydrocarbon receptor involved in ovarian carcinogenesis and chemoresistance
In conclusion, this study provides a better understanding of the the cross-talk between AhR and several other molecular signaling pathways and the role and involvement of AhR in ovarian carcinogenesis and chemoresistance. Moreover, the study suggests that AhR is a potential therapeutic target for OC prevention and maintenance. SIGNIFICANCE: To our knowledge, this is the first study that investigates the role and involvement of AhR and its regulated genes in OC by performing a comparative proteomic analysis to identify the critical proteins with a modulated expression upon AhR activation. We found AhR activation to play a t...
Source: Journal of Proteomics - February 5, 2024 Category: Biochemistry Authors: Lubna Therachiyil Paleerath Peerapen Shahd M Younis Aamir Ahmad Visith Thongboonkerd Shahab Uddin Hesham M Korashy Source Type: research
Lentiviral gene therapy with IGF2-tagged GAA normalizes the skeletal muscle proteome in murine Pompe disease
This study supports the future clinical development of lentiviral gene therapy with LV-IGF2.GAAco as a new treatment option for Pompe disease.PMID:38288553 | DOI:10.1016/j.jprot.2023.105037 (Source: Journal of Proteomics)
Source: Journal of Proteomics - January 30, 2024 Category: Biochemistry Authors: Qiushi Liang Eva C Vlaar Joon M Pijnenburg Erikjan Rijkers Jeroen A A Demmers Arnold G Vulto Ans T van der Ploeg Niek P van Til W W M Pim Pijnappel Source Type: research
Meat Omics: Trends and applications of Omics strategies in meat research
J Proteomics. 2024 Mar 20;295:105090. doi: 10.1016/j.jprot.2024.105090. Epub 2024 Jan 24.NO ABSTRACTPMID:38290411 | DOI:10.1016/j.jprot.2024.105090 (Source: Journal of Proteomics)
Source: Journal of Proteomics - January 30, 2024 Category: Biochemistry Authors: Mohammed Gagaoua Daniel Franco Ranjith Ramanathan Source Type: research
The Eastern Bandy Bandy Vermicella annulata, expresses high abundance of SVMP, CRiSP and Kunitz protein families in its venom proteome
J Proteomics. 2024 Mar 20;295:105086. doi: 10.1016/j.jprot.2024.105086. Epub 2024 Jan 23.ABSTRACTThe Australian elapid snake radiation (Hydrophiinae) has evolved in the absence of competition from other advanced snakes. This has resulted in ecological specialisation in Australian elapids and the potential for venom proteomes divergent to other elapids. We characterised the venom of the Australian elapid Vermicella annulata (eastern bandy bandy). The venom was analysed using a two-dimensional fractionation process consisting of reverse-phase high-performance liquid chromatography then sodium dodecyl sulphate polyacrylamide ...
Source: Journal of Proteomics - January 24, 2024 Category: Biochemistry Authors: Theo Tasoulis C Ruth Wang Joanna Sumner Nathan Dunstan Tara L Pukala Geoffrey K Isbister Source Type: research
5-HT-treated mouse B cells alleviate ulcerative colitis via RIPK1: Insights from proteomic and phosphoproteomic analyses
J Proteomics. 2024 Mar 20;295:105085. doi: 10.1016/j.jprot.2024.105085. Epub 2024 Jan 19.ABSTRACT5-hydroxytryptamine (5-HT) exerts various physiological effects on the intestine through different signaling pathways and molecular transmission mechanisms, including pro- and anti-inflammatory effects. Adoptive transfer of regulatory B cells (Bregs) into colitis mice has exhibited significant therapeutic benefits. We aimed to elucidate the mechanism through which 5-HT-treated B cells alleviate ulcerative colitis. To this end, we analyzed the proteomic and phosphoproteomic profiles of 5-HT-stimulated B cells from naïve mice. W...
Source: Journal of Proteomics - January 21, 2024 Category: Biochemistry Authors: Xiuna Zhang Min Rao Pujun Gao Source Type: research
