Comparative proteomics unveils the bacteriostatic mechanisms of Ga(III) on the regulation of metabolic pathways in Pseudomonas aeruginosa
J Proteomics. 2023 Sep 28:105011. doi: 10.1016/j.jprot.2023.105011. Online ahead of print.ABSTRACTGallium has a long history as a chemotherapeutic agent. The mechanisms of action of Ga(III)-based anti-infectives are different from conventional antibiotics, which primarily result from the chemical similarities of Ga(III) with Fe(III) and substitution of gallium into iron-dependent biological pathways. However, more aspects of the molecular mechanisms of Ga(III) against human pathogens, especially its regulations on bacterial metabolic processes, remain to be understood. Herein, by using conventional quantitative proteomics ...
Source: Journal of Proteomics - September 30, 2023 Category: Biochemistry Authors: Yuchuan Wang Xue Yang Shuo Zhang Jiayi Ai Junteng Wang Junxin Chen Lin Zhao Wanying Wang Haoxin You Source Type: research
Comparative proteomics unveils the bacteriostatic mechanisms of Ga(III) on the regulation of metabolic pathways in Pseudomonas aeruginosa
J Proteomics. 2023 Sep 28:105011. doi: 10.1016/j.jprot.2023.105011. Online ahead of print.ABSTRACTGallium has a long history as a chemotherapeutic agent. The mechanisms of action of Ga(III)-based anti-infectives are different from conventional antibiotics, which primarily result from the chemical similarities of Ga(III) with Fe(III) and substitution of gallium into iron-dependent biological pathways. However, more aspects of the molecular mechanisms of Ga(III) against human pathogens, especially its regulations on bacterial metabolic processes, remain to be understood. Herein, by using conventional quantitative proteomics ...
Source: Journal of Proteomics - September 30, 2023 Category: Biochemistry Authors: Yuchuan Wang Xue Yang Shuo Zhang Jiayi Ai Junteng Wang Junxin Chen Lin Zhao Wanying Wang Haoxin You Source Type: research
Comparative proteomics unveils the bacteriostatic mechanisms of Ga(III) on the regulation of metabolic pathways in Pseudomonas aeruginosa
J Proteomics. 2023 Sep 28:105011. doi: 10.1016/j.jprot.2023.105011. Online ahead of print.ABSTRACTGallium has a long history as a chemotherapeutic agent. The mechanisms of action of Ga(III)-based anti-infectives are different from conventional antibiotics, which primarily result from the chemical similarities of Ga(III) with Fe(III) and substitution of gallium into iron-dependent biological pathways. However, more aspects of the molecular mechanisms of Ga(III) against human pathogens, especially its regulations on bacterial metabolic processes, remain to be understood. Herein, by using conventional quantitative proteomics ...
Source: Journal of Proteomics - September 30, 2023 Category: Biochemistry Authors: Yuchuan Wang Xue Yang Shuo Zhang Jiayi Ai Junteng Wang Junxin Chen Lin Zhao Wanying Wang Haoxin You Source Type: research
The new Ribopeaks (RPK-II): Updated and enlarged tool for bacterial classification based on r-protein m/z data
J Proteomics. 2023 Sep 27:105008. doi: 10.1016/j.jprot.2023.105008. Online ahead of print.ABSTRACTRibopeaks is a rapid, sensitive, and economic web tool for bacterial identification based on m/z data from MALDI-TOF MS. To provide greater accuracy and robustness in the Ribopeaks analyzes we present an updated bacterial identification tool version, called Ribopeaks II (RPK-II). RPK-II contains a larger database, with r-protein data from fully sequenced bacterial genomes and optimized algorithms. Furthermore, this new version provides additional information about the identified bacterium, regarding antibiotic resistance.PMID:...
Source: Journal of Proteomics - September 29, 2023 Category: Biochemistry Authors: Renann Rodrigues da Silva Douglas Tomachewski La ís Priscila Karas Carolina Weigert Galv ão Jos é Carlos Ferreira da Rocha Edmar Miyoshi Rafael Mazer Etto Source Type: research
Enhanced ileum function in weaned piglets via Laminaria digitata and alginate lyase dietary inclusion: A combined proteomics and metabolomics analysis
This study aimed to assess the impact of a 10% dietary inclusion of L. digitata and alginate lyase supplementation on the ileum proteome and metabolome, adopting a hypothesis-generating approach. Findings indicated that control piglets escalated glucose usage as an enteric energy source, as evidenced by the elevated abundance of PKLR and PCK2 proteins and decreased tissue glucose concentration. Additionally, the inclusion of seaweed fostered a rise in proteins linked to enhanced enterocyte structural integrity (ACTBL2, CRMP1, FLII, EML2 and MYLK), elevated peptidase activity (NAALADL1 and CAPNS1), and heightened anti-infla...
Source: Journal of Proteomics - September 29, 2023 Category: Biochemistry Authors: David Miguel Ribeiro C éline C Leclercq Sophie A B Charton M ónica M Costa Daniela Filipa Pires Carvalho Emmanuelle Cocco Kjell Sergeant Jenny Renaut Jo ão Pedro Bengala Freire Jos é António Mestre Prates Andr é Martinho de Almeida Source Type: research
The new Ribopeaks (RPK-II): Updated and enlarged tool for bacterial classification based on r-protein m/z data
J Proteomics. 2023 Sep 27:105008. doi: 10.1016/j.jprot.2023.105008. Online ahead of print.ABSTRACTRibopeaks is a rapid, sensitive, and economic web tool for bacterial identification based on m/z data from MALDI-TOF MS. To provide greater accuracy and robustness in the Ribopeaks analyzes we present an updated bacterial identification tool version, called Ribopeaks II (RPK-II). RPK-II contains a larger database, with r-protein data from fully sequenced bacterial genomes and optimized algorithms. Furthermore, this new version provides additional information about the identified bacterium, regarding antibiotic resistance.PMID:...
Source: Journal of Proteomics - September 29, 2023 Category: Biochemistry Authors: Renann Rodrigues da Silva Douglas Tomachewski La ís Priscila Karas Carolina Weigert Galv ão Jos é Carlos Ferreira da Rocha Edmar Miyoshi Rafael Mazer Etto Source Type: research
Enhanced ileum function in weaned piglets via Laminaria digitata and alginate lyase dietary inclusion: A combined proteomics and metabolomics analysis
This study aimed to assess the impact of a 10% dietary inclusion of L. digitata and alginate lyase supplementation on the ileum proteome and metabolome, adopting a hypothesis-generating approach. Findings indicated that control piglets escalated glucose usage as an enteric energy source, as evidenced by the elevated abundance of PKLR and PCK2 proteins and decreased tissue glucose concentration. Additionally, the inclusion of seaweed fostered a rise in proteins linked to enhanced enterocyte structural integrity (ACTBL2, CRMP1, FLII, EML2 and MYLK), elevated peptidase activity (NAALADL1 and CAPNS1), and heightened anti-infla...
Source: Journal of Proteomics - September 29, 2023 Category: Biochemistry Authors: David Miguel Ribeiro C éline C Leclercq Sophie A B Charton M ónica M Costa Daniela Filipa Pires Carvalho Emmanuelle Cocco Kjell Sergeant Jenny Renaut Jo ão Pedro Bengala Freire Jos é António Mestre Prates Andr é Martinho de Almeida Source Type: research
Goat mammary gland metabolism: An integrated omics analysis to unravel seasonal weight loss tolerance
In this study, data integration of three Omics (transcriptomics, proteomics and metabolomics) was performed using bioinformatic tools, to relate putative biomarkers and evaluate all three levels of information; in a novel approach. This information can enhance selection programs, lowering the impact of SWL on food production systems.PMID:37757955 | DOI:10.1016/j.jprot.2023.105009 (Source: Journal of Proteomics)
Source: Journal of Proteomics - September 27, 2023 Category: Biochemistry Authors: David Miguel Ribeiro Mariana Palma Jos é Salvado Lorenzo E Hern ández-Castellano Juan Capote Noem í Castro Anastasio Arg üello Manolis Matzapetakis Susana S Ara újo Andr é Martinho de Almeida Source Type: research
Goat mammary gland metabolism: An integrated omics analysis to unravel seasonal weight loss tolerance
In this study, data integration of three Omics (transcriptomics, proteomics and metabolomics) was performed using bioinformatic tools, to relate putative biomarkers and evaluate all three levels of information; in a novel approach. This information can enhance selection programs, lowering the impact of SWL on food production systems.PMID:37757955 | DOI:10.1016/j.jprot.2023.105009 (Source: Journal of Proteomics)
Source: Journal of Proteomics - September 27, 2023 Category: Biochemistry Authors: David Miguel Ribeiro Mariana Palma Jos é Salvado Lorenzo E Hern ández-Castellano Juan Capote Noem í Castro Anastasio Arg üello Manolis Matzapetakis Susana S Ara újo Andr é Martinho de Almeida Source Type: research
Beyond the identifiable proteome: Delving into the proteomics of polymyxin-resistant and non-resistant Acinetobacter baumannii from Brazilian hospitals
We present comprehensive proteomic profiles of non-resistant and resistant Acinetobacter baumannii from Brazilian Hospitals strains, and highlight the presence of discriminative and yet unidentified mass spectral clusters. Our work emphasizes the importance of exploring this overlooked data, as it could hold the key to understanding the complex dynamics of antibiotic resistance. This approach not only informs antimicrobial stewardship efforts but also paves the way for the development of innovative diagnostic tools. Thus, our findings have profound implications for the field, as far as methods for providing a new perspecti...
Source: Journal of Proteomics - September 25, 2023 Category: Biochemistry Authors: Amanda Dal Lin Juliana de S da G Fischer Marlon D M Santos Amanda Caroline Camillo-Andrade Louise Ulrich Kurt Tatiana A C B Souza Ana Beatriz Lyrio Lajas Bernardina Rivera Magdalena Portela Rosario Duran Marcelo T ávora Mira Marcelo Pillonetto Paulo Cost Source Type: research
Beyond the identifiable proteome: Delving into the proteomics of polymyxin-resistant and non-resistant Acinetobacter baumannii from Brazilian hospitals
We present comprehensive proteomic profiles of non-resistant and resistant Acinetobacter baumannii from Brazilian Hospitals strains, and highlight the presence of discriminative and yet unidentified mass spectral clusters. Our work emphasizes the importance of exploring this overlooked data, as it could hold the key to understanding the complex dynamics of antibiotic resistance. This approach not only informs antimicrobial stewardship efforts but also paves the way for the development of innovative diagnostic tools. Thus, our findings have profound implications for the field, as far as methods for providing a new perspecti...
Source: Journal of Proteomics - September 25, 2023 Category: Biochemistry Authors: Amanda Dal Lin Juliana de S da G Fischer Marlon D M Santos Amanda Caroline Camillo-Andrade Louise Ulrich Kurt Tatiana A C B Souza Ana Beatriz Lyrio Lajas Bernardina Rivera Magdalena Portela Rosario Duran Marcelo T ávora Mira Marcelo Pillonetto Paulo Cost Source Type: research
Beyond the identifiable proteome: Delving into the proteomics of polymyxin-resistant and non-resistant Acinetobacter baumannii from Brazilian hospitals
We present comprehensive proteomic profiles of non-resistant and resistant Acinetobacter baumannii from Brazilian Hospitals strains, and highlight the presence of discriminative and yet unidentified mass spectral clusters. Our work emphasizes the importance of exploring this overlooked data, as it could hold the key to understanding the complex dynamics of antibiotic resistance. This approach not only informs antimicrobial stewardship efforts but also paves the way for the development of innovative diagnostic tools. Thus, our findings have profound implications for the field, as far as methods for providing a new perspecti...
Source: Journal of Proteomics - September 25, 2023 Category: Biochemistry Authors: Amanda Dal Lin Juliana de S da G Fischer Marlon D M Santos Amanda Caroline Camillo-Andrade Louise Ulrich Kurt Tatiana A C B Souza Ana Beatriz Lyrio Lajas Bernardina Rivera Magdalena Portela Rosario Duran Marcelo T ávora Mira Marcelo Pillonetto Paulo Cost Source Type: research
S-layer is a key element in metabolic response and entry into the stationary phase in Bacillus cereus AH187
In this study, we used high-throughput proteomics to investigate how S-layer synthesis affects the allocation of cellular resources in the emetic B. cereus strain AH187. Our results demonstrate that the synthesis of the S-layer plays a crucial role in the pathogen's ability to thrive under stationary growth phase conditions by modulating the stress response, thereby promoting its lifestyle as an emetic pathogen. We conclude that the synthesis of the S-layer is a critical adaptation for emetic B. cereus to successfully colonize specific niches.PMID:37730087 | DOI:10.1016/j.jprot.2023.105007 (Source: Journal of Proteomics)
Source: Journal of Proteomics - September 20, 2023 Category: Biochemistry Authors: C écile Boutonnet Christian Ginies B éatrice Alpha-Bazin Jean Armengaud Alice Ch âteau Catherine Duport Source Type: research
S-layer is a key element in metabolic response and entry into the stationary phase in Bacillus cereus AH187
In this study, we used high-throughput proteomics to investigate how S-layer synthesis affects the allocation of cellular resources in the emetic B. cereus strain AH187. Our results demonstrate that the synthesis of the S-layer plays a crucial role in the pathogen's ability to thrive under stationary growth phase conditions by modulating the stress response, thereby promoting its lifestyle as an emetic pathogen. We conclude that the synthesis of the S-layer is a critical adaptation for emetic B. cereus to successfully colonize specific niches.PMID:37730087 | DOI:10.1016/j.jprot.2023.105007 (Source: Journal of Proteomics)
Source: Journal of Proteomics - September 20, 2023 Category: Biochemistry Authors: C écile Boutonnet Christian Ginies B éatrice Alpha-Bazin Jean Armengaud Alice Ch âteau Catherine Duport Source Type: research
S-layer is a key element in metabolic response and entry into the stationary phase in Bacillus cereus AH187
In this study, we used high-throughput proteomics to investigate how S-layer synthesis affects the allocation of cellular resources in the emetic B. cereus strain AH187. Our results demonstrate that the synthesis of the S-layer plays a crucial role in the pathogen's ability to thrive under stationary growth phase conditions by modulating the stress response, thereby promoting its lifestyle as an emetic pathogen. We conclude that the synthesis of the S-layer is a critical adaptation for emetic B. cereus to successfully colonize specific niches.PMID:37730087 | DOI:10.1016/j.jprot.2023.105007 (Source: Journal of Proteomics)
Source: Journal of Proteomics - September 20, 2023 Category: Biochemistry Authors: C écile Boutonnet Christian Ginies B éatrice Alpha-Bazin Jean Armengaud Alice Ch âteau Catherine Duport Source Type: research
