Quantitative phosphoproteomics explain cryopreservation-induced reductions in ram sperm motility
This study used phosphorylated TMT quantitative proteomics to explore regulation of epigenetic modifications in frozen-thawed ram sperm. This experiment demonstrated that ram sperm freezing affects phosphorylation site modifications of proteins, especially those related to functions such as sperm motility and energy production. Furthermore, it is important to link functions of phosphorylated proteins with changes in sperm quality after freezing and thawing, and to clarify intrinsic reasons for sperm quality changes, which is of great importance for elucidating mechanisms of sperm freezing damage. Based on these protein mar...
Source: Journal of Proteomics - March 4, 2024 Category: Biochemistry Authors: Shengqin Zang Xiaorui Yang Jiangfeng Ye Xianhong Mo Guangbin Zhou Yi Fang Source Type: research
Integrative transcriptomic and proteomic profile revealed inhibition of oxidative phosphorylation and peroxisomes during renal interstitial fibrosis
J Proteomics. 2024 Feb 29:105144. doi: 10.1016/j.jprot.2024.105144. Online ahead of print.ABSTRACTEffective therapies of chronic kidney disease (CKD) are lacking due to the unclear molecular pathogenesis. Previous single omics-studies have described potential molecular regulation mechanism of CKD only at the level of transcription or translation. Therefore, this study generated an integrated transcriptomic and proteomic profile to provide deep insights into the continuous transcription-translation process during CKD. The comprehensive datasets identified 14,948 transcripts and 6423 proteins, 233 up-regulated and 364 down-r...
Source: Journal of Proteomics - March 2, 2024 Category: Biochemistry Authors: Cheng Wang Zhuo Pan Linxiao Sun Qiangqiang Li Source Type: research
Multiomics unravels potential molecular switches in the C < sub > 3 < /sub > to CAM transition of Mesembryanthemum crystallinum
J Proteomics. 2024 Feb 29:105145. doi: 10.1016/j.jprot.2024.105145. Online ahead of print.ABSTRACTMesembryanthemum crystallinum (common ice plant), a facultative CAM plant, shifts from C3 to CAM photosynthesis under salt stress, enhancing water use efficiency. Here we used transcriptomics, proteomics, and targeted metabolomics to profile molecular changes during the diel cycle of C3 to CAM transition. The results confirmed expected changes associated with CAM photosynthesis, starch biosynthesis and degradation, and glycolysis/gluconeogenesis. Importantly, they yielded new discoveries: 1) Transcripts displayed greater circa...
Source: Journal of Proteomics - March 2, 2024 Category: Biochemistry Authors: Qijie Guan Wenwen Kong Bowen Tan Wei Zhu Tahmina Aker Jing Li Jingkui Tian Sixue Chen Source Type: research
Integrative transcriptomic and proteomic profile revealed inhibition of oxidative phosphorylation and peroxisomes during renal interstitial fibrosis
J Proteomics. 2024 Feb 29:105144. doi: 10.1016/j.jprot.2024.105144. Online ahead of print.ABSTRACTEffective therapies of chronic kidney disease (CKD) are lacking due to the unclear molecular pathogenesis. Previous single omics-studies have described potential molecular regulation mechanism of CKD only at the level of transcription or translation. Therefore, this study generated an integrated transcriptomic and proteomic profile to provide deep insights into the continuous transcription-translation process during CKD. The comprehensive datasets identified 14,948 transcripts and 6423 proteins, 233 up-regulated and 364 down-r...
Source: Journal of Proteomics - March 2, 2024 Category: Biochemistry Authors: Cheng Wang Zhuo Pan Linxiao Sun Qiangqiang Li Source Type: research
Multiomics unravels potential molecular switches in the C < sub > 3 < /sub > to CAM transition of Mesembryanthemum crystallinum
J Proteomics. 2024 Feb 29:105145. doi: 10.1016/j.jprot.2024.105145. Online ahead of print.ABSTRACTMesembryanthemum crystallinum (common ice plant), a facultative CAM plant, shifts from C3 to CAM photosynthesis under salt stress, enhancing water use efficiency. Here we used transcriptomics, proteomics, and targeted metabolomics to profile molecular changes during the diel cycle of C3 to CAM transition. The results confirmed expected changes associated with CAM photosynthesis, starch biosynthesis and degradation, and glycolysis/gluconeogenesis. Importantly, they yielded new discoveries: 1) Transcripts displayed greater circa...
Source: Journal of Proteomics - March 2, 2024 Category: Biochemistry Authors: Qijie Guan Wenwen Kong Bowen Tan Wei Zhu Tahmina Aker Jing Li Jingkui Tian Sixue Chen Source Type: research
The proteome of Penicillium expansum during infection of postharvest apple is revealed using Label-Free and Parallel Reaction Monitoring(PRM)Techniques
This study provides the foundation for relevant postharvest control strategies, paving the way for further exploration of the proteome of pathogens infecting fruit and vegetables. SIGNIFICANCE: Proteins are macromolecules essential to the life of organisms, as they participate in the function and structure of cells. Proteomics technology is currently one of the important means to study the the response mechanism of pathogenic bacteria to plant infection, which can reveal the essence of physiological and pathological processes and help to clarify the possible relationship between protein abundance and plant stress. The pres...
Source: Journal of Proteomics - March 1, 2024 Category: Biochemistry Authors: Kaili Wang Hua Wang Meng Xu Guillaume Legrand Ngolong Ngea Hongyin Zhang Source Type: research
The proteome of Penicillium expansum during infection of postharvest apple is revealed using Label-Free and Parallel Reaction Monitoring(PRM)Techniques
This study provides the foundation for relevant postharvest control strategies, paving the way for further exploration of the proteome of pathogens infecting fruit and vegetables. SIGNIFICANCE: Proteins are macromolecules essential to the life of organisms, as they participate in the function and structure of cells. Proteomics technology is currently one of the important means to study the the response mechanism of pathogenic bacteria to plant infection, which can reveal the essence of physiological and pathological processes and help to clarify the possible relationship between protein abundance and plant stress. The pres...
Source: Journal of Proteomics - March 1, 2024 Category: Biochemistry Authors: Kaili Wang Hua Wang Meng Xu Guillaume Legrand Ngolong Ngea Hongyin Zhang Source Type: research
Digging deeper into ancient skeletal proteomes through consecutive digestion with multiple proteases
J Proteomics. 2024 Feb 27:105143. doi: 10.1016/j.jprot.2024.105143. Online ahead of print.ABSTRACTAn increasing number of studies utilise the recovery of ancient skeletal proteomes for phylogenetic and evolutionary analysis. Although these studies manage to extract and analyse ancient peptides, the recovered proteomes are generally small in size and with low protein sequence coverage. We expand on previous observations which have shown that the parallel digestion and analysis of Pleistocene skeletal proteomes increases overall proteome size and protein sequence coverage. Furthermore, we demonstrate that the consecutive dig...
Source: Journal of Proteomics - February 29, 2024 Category: Biochemistry Authors: Zandra Fagern äs Gaudry Troch é Jesper V Olsen Frido Welker Source Type: research
Digging deeper into ancient skeletal proteomes through consecutive digestion with multiple proteases
J Proteomics. 2024 Feb 27:105143. doi: 10.1016/j.jprot.2024.105143. Online ahead of print.ABSTRACTAn increasing number of studies utilise the recovery of ancient skeletal proteomes for phylogenetic and evolutionary analysis. Although these studies manage to extract and analyse ancient peptides, the recovered proteomes are generally small in size and with low protein sequence coverage. We expand on previous observations which have shown that the parallel digestion and analysis of Pleistocene skeletal proteomes increases overall proteome size and protein sequence coverage. Furthermore, we demonstrate that the consecutive dig...
Source: Journal of Proteomics - February 29, 2024 Category: Biochemistry Authors: Zandra Fagern äs Gaudry Troch é Jesper V Olsen Frido Welker Source Type: research
Decoding the influence of semen collection processes on goat sperm quality from a perspective of seminal plasma proteomics
In conclusion, this study provides valuable insights into the effects of semen collection on goat semen quality and explores the potential action mechanism based on the modification of SP proteomes. SIGNIFICANCE OF THE STUDY: The quality of fresh semen directly influences the results of artificial insemination and semen cryopreservation in livestock. This study represents the first attempt to evaluate the impact of semen collection methods including electroejaculation and artificial vagina on sperm quality and seminal plasma proteomes in goat. The results of this study demonstrated that semen collection methods directly im...
Source: Journal of Proteomics - February 26, 2024 Category: Biochemistry Authors: Chunrong Lv Allai Larbi Chunyan Li Jiangchong Liang Guoquan Wu Qingyong Shao Quobo Quan Source Type: research
Decoding the influence of semen collection processes on goat sperm quality from a perspective of seminal plasma proteomics
In conclusion, this study provides valuable insights into the effects of semen collection on goat semen quality and explores the potential action mechanism based on the modification of SP proteomes. SIGNIFICANCE OF THE STUDY: The quality of fresh semen directly influences the results of artificial insemination and semen cryopreservation in livestock. This study represents the first attempt to evaluate the impact of semen collection methods including electroejaculation and artificial vagina on sperm quality and seminal plasma proteomes in goat. The results of this study demonstrated that semen collection methods directly im...
Source: Journal of Proteomics - February 26, 2024 Category: Biochemistry Authors: Chunrong Lv Allai Larbi Chunyan Li Jiangchong Liang Guoquan Wu Qingyong Shao Quobo Quan Source Type: research
Decoding the influence of semen collection processes on goat sperm quality from a perspective of seminal plasma proteomics
In conclusion, this study provides valuable insights into the effects of semen collection on goat semen quality and explores the potential action mechanism based on the modification of SP proteomes. SIGNIFICANCE OF THE STUDY: The quality of fresh semen directly influences the results of artificial insemination and semen cryopreservation in livestock. This study represents the first attempt to evaluate the impact of semen collection methods including electroejaculation and artificial vagina on sperm quality and seminal plasma proteomes in goat. The results of this study demonstrated that semen collection methods directly im...
Source: Journal of Proteomics - February 26, 2024 Category: Biochemistry Authors: Chunrong Lv Allai Larbi Chunyan Li Jiangchong Liang Guoquan Wu Qingyong Shao Quobo Quan Source Type: research
Proteomic analysis of multiple organ dysfunction induced by rhabdomyolysis
This study comprehensively describes the functional and structural changes of kidney, heart and lung in rats after rhabdomyolysis, analyzes the DEPs of kidney, heart and lung, and determines the key role of Serpina3n in multiple organ injury caused by rhabdomyolysis. SIGNIFICANCE: This study comprehensively describes the functional and structural changes of kidney, heart and lung in rats after rhabdomyolysis, analyzes the DEPs of kidney, heart and lung, and determines the key role of Serpina3n in multiple organ injury caused by rhabdomyolysis.PMID:38403185 | DOI:10.1016/j.jprot.2024.105138 (Source: Journal of Proteomics)
Source: Journal of Proteomics - February 25, 2024 Category: Biochemistry Authors: Yan Zhang Qiang Lyu Xiao Han Xu Wang Ran Liu Jing Hao Li Zhang Xiang-Mei Chen Source Type: research
Proteomic analysis of multiple organ dysfunction induced by rhabdomyolysis
This study comprehensively describes the functional and structural changes of kidney, heart and lung in rats after rhabdomyolysis, analyzes the DEPs of kidney, heart and lung, and determines the key role of Serpina3n in multiple organ injury caused by rhabdomyolysis. SIGNIFICANCE: This study comprehensively describes the functional and structural changes of kidney, heart and lung in rats after rhabdomyolysis, analyzes the DEPs of kidney, heart and lung, and determines the key role of Serpina3n in multiple organ injury caused by rhabdomyolysis.PMID:38403185 | DOI:10.1016/j.jprot.2024.105138 (Source: Journal of Proteomics)
Source: Journal of Proteomics - February 25, 2024 Category: Biochemistry Authors: Yan Zhang Qiang Lyu Xiao Han Xu Wang Ran Liu Jing Hao Li Zhang Xiang-Mei Chen Source Type: research
Comparative proteomic analysis of seed germination between allotetraploid cotton Gossypium hirsutum and Gossypium barbadense
This study helps to elucidate the evolution and domestication history of cotton polyploids and may allow breeders to understand their domestication history better and improve fiber quality and adaptability.PMID:38401592 | DOI:10.1016/j.jprot.2024.105130 (Source: Journal of Proteomics)
Source: Journal of Proteomics - February 24, 2024 Category: Biochemistry Authors: Aishuake Huwanixi Zhao Peng Shenglan Li Yutian Zhou Sixian Zhao Cuihong Wan Source Type: research
