The Eastern Bandy Bandy Vermicella annulata, expresses high abundance of SVMP, CRiSP and Kunitz protein families in its venom proteome
J Proteomics. 2024 Mar 20;295:105086. doi: 10.1016/j.jprot.2024.105086. Epub 2024 Jan 23.ABSTRACTThe Australian elapid snake radiation (Hydrophiinae) has evolved in the absence of competition from other advanced snakes. This has resulted in ecological specialisation in Australian elapids and the potential for venom proteomes divergent to other elapids. We characterised the venom of the Australian elapid Vermicella annulata (eastern bandy bandy). The venom was analysed using a two-dimensional fractionation process consisting of reverse-phase high-performance liquid chromatography then sodium dodecyl sulphate polyacrylamide ...
Source: Journal of Proteomics - January 24, 2024 Category: Biochemistry Authors: Theo Tasoulis C Ruth Wang Joanna Sumner Nathan Dunstan Tara L Pukala Geoffrey K Isbister Source Type: research
5-HT-treated mouse B cells alleviate ulcerative colitis via RIPK1: Insights from proteomic and phosphoproteomic analyses
J Proteomics. 2024 Mar 20;295:105085. doi: 10.1016/j.jprot.2024.105085. Epub 2024 Jan 19.ABSTRACT5-hydroxytryptamine (5-HT) exerts various physiological effects on the intestine through different signaling pathways and molecular transmission mechanisms, including pro- and anti-inflammatory effects. Adoptive transfer of regulatory B cells (Bregs) into colitis mice has exhibited significant therapeutic benefits. We aimed to elucidate the mechanism through which 5-HT-treated B cells alleviate ulcerative colitis. To this end, we analyzed the proteomic and phosphoproteomic profiles of 5-HT-stimulated B cells from naïve mice. W...
Source: Journal of Proteomics - January 21, 2024 Category: Biochemistry Authors: Xiuna Zhang Min Rao Pujun Gao Source Type: research
Profiling the neuroproteomics of honeybee brain: A clue for understanding the role of neuropeptides in the modulation of aggressivity
J Proteomics. 2024 Mar 20;295:105089. doi: 10.1016/j.jprot.2024.105089. Epub 2024 Jan 19.ABSTRACTThe aggressivity is modulated in honeybee brain through a series of actions in cascade mode, with the participation of the neuropeptides AmAST A (59-76) and AmTRP (254-262). The aggressivity of honeybees was stimulated by injecting both neuropeptides in the hemocoel of the worker honeybees, which were submitted to behavioral assays of aggression. The brain of stinger individuals were removed by dissection and submitted to proteomic analysis; shotgun proteomic approach of honeybee brain revealed that both neuropeptides activate ...
Source: Journal of Proteomics - January 21, 2024 Category: Biochemistry Authors: Gabriela Mendon ça Paula Anally R da Silva Menegasso Jos é Roberto Aparecido Dos-Santos-Pinto Osmar Malaspina Mario Sergio Palma Source Type: research
Proteomic analysis of the mucus of the photosynthetic sea slug Elysia crispata
This study presents the first comprehensive analysis of the mucus proteome of the sea slug E. crispata using gel electrophoresis and HPLC-MS/MS. We identified 306 proteins in the mucus secretions of this animal, despite the limited entries for E. crispata in the Uniprot database. The functional annotation of the mucus proteome using Gene Ontology identified proteins involved in different functions such as hydrolase activity (molecular function), carbohydrate-derived metabolic processes (biological processes) and cytoskeletal organization (cell component). Moreover, a high proportion of proteins with enzymatic activity in t...
Source: Journal of Proteomics - January 18, 2024 Category: Biochemistry Authors: Diana Lopes Susana S Aveiro S ónia Cruz Paulo Cartaxana Pedro Domingues Source Type: research
The protein map of the protozoan parasite Leishmania (Leishmania) amazonensis, Leishmania (Viannia) braziliensis and Leishmania (Leishmania) infantum during growth phase transition and temperature stress
This study provides an in-depth proteome analysis of three Leishmania spp., and contributes compelling evidence of the molecular alterations of these parasites in conditions mimicking the interaction of the parasites with the insect vector and vertebrate hosts. SIGNIFICANCE: Leishmaniasis disease manifests in two main clinical forms according to the infecting Leishmania species and host immune responses, cutaneous and visceral leishmaniasis. In Brazil, cutaneous leishmaniasis (CL) is associated with L. braziliensis and L. amazonensis, while visceral leishmaniasis, also called kala-azar, is caused by L. infantum. Leishmania...
Source: Journal of Proteomics - January 18, 2024 Category: Biochemistry Authors: Simon Ngao Mule Joyce Silva Saad Ismael Pretto Sauter Livia Rosa Fernandes Gilberto Santos de Oliveira Daniel Quina Fabia Tomie Tano Deborah Brandt-Almeida Gabriel Padr ón Beatriz Simonsen Stolf Martin R Larsen Mauro Cortez Giuseppe Palmisano Source Type: research
Corrigendum to "Determination of the heterogeneous interactome between Edwardsiella tarda and fish gills" [Journal of Proteomics, Volume 75, Issue 4, 2 February 2012, Pages 1119-1128]
J Proteomics. 2024 Mar 15;294:105082. doi: 10.1016/j.jprot.2024.105082. Epub 2024 Jan 18.NO ABSTRACTPMID:38238195 | DOI:10.1016/j.jprot.2024.105082 (Source: Journal of Proteomics)
Source: Journal of Proteomics - January 18, 2024 Category: Biochemistry Authors: Yang Liu Haili Zhang Yongjin Liu Hui Li Xuanxian Peng Source Type: research
Proteomic and molecular analyses to understand the promotive effect of safranal on soybean growth under salt stress
J Proteomics. 2024 Mar 15;294:105072. doi: 10.1016/j.jprot.2024.105072. Epub 2024 Jan 12.ABSTRACTSafranal is a free radical scavenger and useful as an antioxidant molecule; however, its promotive role in soybean is not explored. Salt stress decreased soybean growth and safranal improved it even if under salt stress. To study the positive mechanism of safranal on soybean growth, a proteomic approach was used. According to functional categorization, oppositely changed proteins were further confirmed using biochemical techniques. Actin and calcium-dependent protein kinase decreased in soybean root and hypocotyl, respectively,...
Source: Journal of Proteomics - January 13, 2024 Category: Biochemistry Authors: Rehana Kausar Takumi Nishiuchi Setsuko Komatsu Source Type: research
Millmeter-wave irradiation regulates mRNA-expression and the ubiquitin-proteasome system in wheat exposed to flooding stress
J Proteomics. 2024 Mar 15;294:105073. doi: 10.1016/j.jprot.2024.105073. Epub 2024 Jan 11.ABSTRACTThe irradiation with millimeter-wave (MMW) of wheat seeds promotes root growth under flooding stress; however, its role is not completely clarified. Nuclear proteomics was performed, to reveal the role of MMW irradiation in enhancing flooding tolerance. The purity of nuclear fractions purified from roots was verified. Histone, which is a protein marker for nuclear-purification efficiency, was enriched; and cytosolic ascorbate peroxidase was reduced in the nuclear fraction. The principal-component analysis of proteome displayed ...
Source: Journal of Proteomics - January 13, 2024 Category: Biochemistry Authors: Setsuko Komatsu Takumi Nishiuchi Takashi Furuya Masahiko Tani Source Type: research
Label-free comparative proteomic analysis of Pleurotus eryngii grown on sawdust, bagasse, and peanut shell substrates
In this study, the secretomes of P. eryngii grown on four different carbon sources were compared. The findings revealed the extracellular enzymes implicated in the degradation of lignocellulose, offering avenues for further investigation into the biotransformation mechanisms of P. eryngii biomass and the potential utilization of agricultural wastes. SIGNIFICANCE: The cost of the substrate for mushroom cultivation has increased as the production of edible fungus has risen year after year. Therefore, the use of these locally available lignocellulosic wastes as substrates offers a cost-cutting option. Further, the overuse of ...
Source: Journal of Proteomics - January 10, 2024 Category: Biochemistry Authors: Zihao Li Cuimin Zhao Yuanyuan Zhou Suyue Zheng Qingxiu Hu Yajie Zou Source Type: research
The molecular response of Mytilus coruscus mantle to shell damage under acute acidified sea water revealed by iTRAQ based quantitative proteomic analysis
J Proteomics. 2024 Mar 15;294:105062. doi: 10.1016/j.jprot.2023.105062. Epub 2023 Dec 27.ABSTRACTMytilus coruscus is an economically important marine bivalve that lives in estuarine sea areas with seasonal coastal acidification and frequently suffers shell injury in the natural environment. However, the molecular responses and biochemical properties of Mytilus under these conditions are not fully understood. In the present study, we employed tandem mass spectrometry combined with isobaric tagging to identify differentially expressed proteins in the mantle tissue of M. coruscus under different short-term treatments, includi...
Source: Journal of Proteomics - December 29, 2023 Category: Biochemistry Authors: Yingao Li Zhi Liao Xiaojun Fan Ying Wang Fei Liu Xiaolin Zhang Jianyu He Isabella Buttino Xiaojun Yan Changsheng Tang Source Type: research
DIA-based quantitative proteomic analysis of porcine endometrium in the peri-implantation phase
This study will help to better reveal the related proteins involved in embryo implantation, and lay a foundation for further research on the mechanism of endometrial regulation of embryo implantation. SIGNIFICANCE OF THE STUDY: The 12th day of gestation is an important point in the peri-implantation period of pigs, when the endometrium presents a receptive state under the stimulation of estrogen. DIA proteomics technology is an emerging protein identification technology in recent years, which can obtain protein information through comprehensive and unbiased scanning. In this study, DIA technology was used to characterize e...
Source: Journal of Proteomics - December 29, 2023 Category: Biochemistry Authors: Chen Zhou Yongzhong Wang Simin He Shifei Lin Jie Cheng Qun Hu Fanming Meng Ting Gu Gengyuan Cai Zicong Li Zhenfang Wu Linjun Hong Source Type: research
The molecular response of Mytilus coruscus mantle to shell damage under acute acidified sea water revealed by iTRAQ based quantitative proteomic analysis
J Proteomics. 2023 Dec 27:105062. doi: 10.1016/j.jprot.2023.105062. Online ahead of print.ABSTRACTMytilus coruscus is an economically important marine bivalve that lives in estuarine sea areas with seasonal coastal acidification and frequently suffers shell injury in the natural environment. However, the molecular responses and biochemical properties of Mytilus under these conditions are not fully understood. In the present study, we employed tandem mass spectrometry combined with isobaric tagging to identify differentially expressed proteins in the mantle tissue of M. coruscus under different short-term treatments, includ...
Source: Journal of Proteomics - December 29, 2023 Category: Biochemistry Authors: Yingao Li Zhi Liao Xiaojun Fan Ying Wang Fei Liu Xiaolin Zhang Jianyu He Isabella Buttino Xiaojun Yan Changsheng Tang Source Type: research
DIA-based quantitative proteomic analysis of porcine endometrium in the peri-implantation phase
This study will help to better reveal the related proteins involved in embryo implantation, and lay a foundation for further research on the mechanism of endometrial regulation of embryo implantation. SIGNIFICANCE OF THE STUDY: The 12th day of gestation is an important point in the peri-implantation period of pigs, when the endometrium presents a receptive state under the stimulation of estrogen. DIA proteomics technology is an emerging protein identification technology in recent years, which can obtain protein information through comprehensive and unbiased scanning. In this study, DIA technology was used to characterize e...
Source: Journal of Proteomics - December 29, 2023 Category: Biochemistry Authors: Chen Zhou Yongzhong Wang Simin He Shifei Lin Jie Cheng Qun Hu Fanming Meng Ting Gu Gengyuan Cai Zicong Li Zhenfang Wu Linjun Hong Source Type: research
The molecular response of Mytilus coruscus mantle to shell damage under acute acidified sea water revealed by iTRAQ based quantitative proteomic analysis
J Proteomics. 2023 Dec 27:105062. doi: 10.1016/j.jprot.2023.105062. Online ahead of print.ABSTRACTMytilus coruscus is an economically important marine bivalve that lives in estuarine sea areas with seasonal coastal acidification and frequently suffers shell injury in the natural environment. However, the molecular responses and biochemical properties of Mytilus under these conditions are not fully understood. In the present study, we employed tandem mass spectrometry combined with isobaric tagging to identify differentially expressed proteins in the mantle tissue of M. coruscus under different short-term treatments, includ...
Source: Journal of Proteomics - December 29, 2023 Category: Biochemistry Authors: Yingao Li Zhi Liao Xiaojun Fan Ying Wang Fei Liu Xiaolin Zhang Jianyu He Isabella Buttino Xiaojun Yan Changsheng Tang Source Type: research
DIA-based quantitative proteomic analysis of porcine endometrium in the peri-implantation phase
This study will help to better reveal the related proteins involved in embryo implantation, and lay a foundation for further research on the mechanism of endometrial regulation of embryo implantation. SIGNIFICANCE OF THE STUDY: The 12th day of gestation is an important point in the peri-implantation period of pigs, when the endometrium presents a receptive state under the stimulation of estrogen. DIA proteomics technology is an emerging protein identification technology in recent years, which can obtain protein information through comprehensive and unbiased scanning. In this study, DIA technology was used to characterize e...
Source: Journal of Proteomics - December 29, 2023 Category: Biochemistry Authors: Chen Zhou Yongzhong Wang Simin He Shifei Lin Jie Cheng Qun Hu Fanming Meng Ting Gu Gengyuan Cai Zicong Li Zhenfang Wu Linjun Hong Source Type: research
