Proteomics and phosphoproteomics reveal the different drought-responsive mechanisms of priming with (Z)-3-hexenyl acetate in two tea cultivars

This study investigated the physiological, proteomic, and phosphoproteomic profiling of two tea plant varieties of Longjingchangye (LJCY) and Zhongcha 108 (ZC108) with contrasting drought tolerance characteristics under drought stress. Physiological data showed that spraying Z-3-HAC exhibited higher activities of superoxide dismutase (SOD) and catalase (CAT) in both LJCY and ZC108 but lower content of malondialdehyde (MDA) in LJCY under drought stress. The proteomic and phosphoproteomic analysis suggested that the drought tolerance mechanism of Z-3-HAC in LJCY and ZC108 was different. Proteomic analyses revealed that Z-3-HAC enhanced the drought tolerance of LJCY by fructose metabolism while enhancing the drought tolerance of ZC108 by promoting glucan biosynthesis and galactose metabolism. Furthermore, the differential abundance phosphoproteins (DAPPs) related to intracellular protein transmembrane transport and protein transmembrane transport were enriched in LJCY, and the regulation of response to osmotic stress and regulation of mRNA processing were enriched in ZC108. In addition, protein-phosphoprotein interactions (PPI) analyses suggested that energy metabolism and starch and sucrose metabolic processes might play critical roles in LJCY and ZC108, respectively. These results will help to understand the mechanisms by which Z-3-HAC enhances the drought resistance of tea plants at the protein level. SIGNIFICANT: Green leaf volatiles (GLVs) are important volatile organic com...

Source: Journal of Proteomics - October 5, 2023 Category: Biochemistry Authors: Shuangshuang Wang Honglian Gu Sizhou Chen Yuchen Li Jiazhi Shen Yu Wang Zhaotang Ding Source Type: research