Proteomic and metabolomic insights into seed germination of Ferula assa-foetida
J Proteomics. 2024 Apr 9;300:105176. doi: 10.1016/j.jprot.2024.105176. Online ahead of print.ABSTRACTCold stratification is known to affect the speed of seed germination; however, its regulation at the molecular level in Ferula assa-foetida remains ambiguous. Here, we used cold stratification (4 °C in the dark) to induce germination in F. assa-foetida and adopted a proteomic and metabolomic approach to understand the molecular mechanism of germination. Compared to the control, we identified 209 non-redundant proteins and 96 metabolites in germinated F. assa-foetida seed. Results highlight the common and unique regulatory ...
Source: Journal of Proteomics - April 11, 2024 Category: Biochemistry Authors: Ashwani Punia Manglesh Kumari Monika Chouhan Vishal Saini Robin Joshi Ashok Kumar Rajiv Kumar Source Type: research
Proteomic and metabolomic insights into seed germination of Ferula assa-foetida
J Proteomics. 2024 Apr 9:105176. doi: 10.1016/j.jprot.2024.105176. Online ahead of print.ABSTRACTCold stratification is known to affect the speed of seed germination; however, its regulation at the molecular level in Ferula assa-foetida remains ambiguous. Here, we used cold stratification (4 °C in the dark) to induce germination in Ferula assa-foetida and adopted a proteomic and metabolomic approach to understand the molecular mechanism of germination. Compared to the control, we identified 209 non-redundant proteins and 96 metabolites in germinated F. assa-foetida seed. Results highlight the common and unique regulatory ...
Source: Journal of Proteomics - April 11, 2024 Category: Biochemistry Authors: Ashwani Punia Manglesh Kumari Monika Chouhan Vishal Saini Robin Joshi Ashok Kumar Rajiv Kumar Source Type: research
Proteomic and metabolomic insights into seed germination of Ferula assa-foetida
J Proteomics. 2024 Apr 9:105176. doi: 10.1016/j.jprot.2024.105176. Online ahead of print.ABSTRACTCold stratification is known to affect the speed of seed germination; however, its regulation at the molecular level in Ferula assa-foetida remains ambiguous. Here, we used cold stratification (4 °C in the dark) to induce germination in Ferula assa-foetida and adopted a proteomic and metabolomic approach to understand the molecular mechanism of germination. Compared to the control, we identified 209 non-redundant proteins and 96 metabolites in germinated F. assa-foetida seed. Results highlight the common and unique regulatory ...
Source: Journal of Proteomics - April 11, 2024 Category: Biochemistry Authors: Ashwani Punia Manglesh Kumari Monika Chouhan Vishal Saini Robin Joshi Ashok Kumar Rajiv Kumar Source Type: research
A candidate panel of eight urinary proteins shows potential of early diagnosis and risk assessment for diabetic kidney disease in type 1 diabetes
J Proteomics. 2024 Apr 2;300:105167. doi: 10.1016/j.jprot.2024.105167. Online ahead of print.ABSTRACTDiabetic kidney disease (DKD) poses a significant health challenge for individuals with diabetes. At its initial stages, DKD often presents asymptomatically, and the standard for non-invasive diagnosis, the albumin-creatinine ratio (ACR), employs discrete categorizations (normal, microalbuminuria, macroalbuminuria) with limitations in sensitivity and specificity across diverse population cohorts. Single biomarker reliance further restricts the predictive value in clinical settings. Given the escalating prevalence of diabete...
Source: Journal of Proteomics - April 4, 2024 Category: Biochemistry Authors: Jeremy Altman Shan Bai Sharad Purohit John White Dennis Steed Su Liu Diane Hopkins Jin-Xiong She Ashok Sharma Wenbo Zhi Source Type: research
Use of data-independent acquisition mass spectrometry to identify an objective serum indicator of the need for osteoporotic therapeutic intervention
This study aimed to identify objective serum indicators of the need for therapeutic intervention in individuals at risk of osteoporotic fracture. Comprehensive proteome analyses of serum collected from patients with osteoporosis but no fractures, patients with osteopenia and fragility fractures, and controls were performed by data-independent acquisition mass spectrometry. Collective evaluation of the proteome analysis data and ELISA-based assays identified serum ECM1 as a potential objective marker of the risk of fragility fractures in patients with osteoporosis or osteopenia. The findings are an important step toward the...
Source: Journal of Proteomics - April 4, 2024 Category: Biochemistry Authors: Yusuke Nakai Ken Kumagai Yoko Ino Tomoko Akiyama Kayano Moriyama Yuriko Takeda Kenji Egashira Takashi Ohira Akihide Ryo Tomoyuki Saito Yutaka Inaba Hisashi Hirano Yayoi Kimura Source Type: research
A candidate panel of eight urinary proteins shows potential of early diagnosis and risk assessment for diabetic kidney disease in type 1 diabetes
J Proteomics. 2024 Apr 2;300:105167. doi: 10.1016/j.jprot.2024.105167. Online ahead of print.ABSTRACTDiabetic kidney disease (DKD) poses a significant health challenge for individuals with diabetes. At its initial stages, DKD often presents asymptomatically, and the standard for non-invasive diagnosis, the albumin-creatinine ratio (ACR), employs discrete categorizations (normal, microalbuminuria, macroalbuminuria) with limitations in sensitivity and specificity across diverse population cohorts. Single biomarker reliance further restricts the predictive value in clinical settings. Given the escalating prevalence of diabete...
Source: Journal of Proteomics - April 4, 2024 Category: Biochemistry Authors: Jeremy Altman Shan Bai Sharad Purohit John White Dennis Steed Su Liu Diane Hopkins Jin-Xiong She Ashok Sharma Wenbo Zhi Source Type: research
Use of data-independent acquisition mass spectrometry to identify an objective serum indicator of the need for osteoporotic therapeutic intervention
This study aimed to identify objective serum indicators of the need for therapeutic intervention in individuals at risk of osteoporotic fracture. Comprehensive proteome analyses of serum collected from patients with osteoporosis but no fractures, patients with osteopenia and fragility fractures, and controls were performed by data-independent acquisition mass spectrometry. Collective evaluation of the proteome analysis data and ELISA-based assays identified serum ECM1 as a potential objective marker of the risk of fragility fractures in patients with osteoporosis or osteopenia. The findings are an important step toward the...
Source: Journal of Proteomics - April 4, 2024 Category: Biochemistry Authors: Yusuke Nakai Ken Kumagai Yoko Ino Tomoko Akiyama Kayano Moriyama Yuriko Takeda Kenji Egashira Takashi Ohira Akihide Ryo Tomoyuki Saito Yutaka Inaba Hisashi Hirano Yayoi Kimura Source Type: research
A candidate panel of eight urinary proteins shows potential of early diagnosis and risk assessment for diabetic kidney disease in type 1 diabetes
J Proteomics. 2024 Apr 2:105167. doi: 10.1016/j.jprot.2024.105167. Online ahead of print.ABSTRACTDiabetic kidney disease (DKD) poses a significant health challenge for individuals with diabetes. At its initial stages, DKD often presents asymptomatically, and the standard for non-invasive diagnosis, the albumin-creatinine ratio (ACR), employs discrete categorizations (normal, microalbuminuria, macroalbuminuria) with limitations in sensitivity and specificity across diverse population cohorts. Single biomarker reliance further restricts the predictive value in clinical settings. Given the escalating prevalence of diabetes, o...
Source: Journal of Proteomics - April 4, 2024 Category: Biochemistry Authors: Jeremy Altman Shan Bai Sharad Purohit John White Dennis Steed Su Liu Diane Hopkins Jin-Xiong She Ashok Sharma Wenbo Zhi Source Type: research
Use of data-independent acquisition mass spectrometry to identify an objective serum indicator of the need for osteoporotic therapeutic intervention
This study aimed to identify objective serum indicators of the need for therapeutic intervention in individuals at risk of osteoporotic fracture. Comprehensive proteome analyses of serum collected from patients with osteoporosis but no fractures, patients with osteopenia and fragility fractures, and controls were performed by data-independent acquisition mass spectrometry. Collective evaluation of the proteome analysis data and ELISA-based assays identified serum ECM1 as a potential objective marker of the risk of fragility fractures in patients with osteoporosis or osteopenia. The findings are an important step toward the...
Source: Journal of Proteomics - April 4, 2024 Category: Biochemistry Authors: Yusuke Nakai Ken Kumagai Yoko Ino Tomoko Akiyama Kayano Moriyama Yuriko Takeda Kenji Egashira Takashi Ohira Akihide Ryo Tomoyuki Saito Yutaka Inaba Hisashi Hirano Yayoi Kimura Source Type: research
A candidate panel of eight urinary proteins shows potential of early diagnosis and risk assessment for diabetic kidney disease in type 1 diabetes
J Proteomics. 2024 Apr 2:105167. doi: 10.1016/j.jprot.2024.105167. Online ahead of print.ABSTRACTDiabetic kidney disease (DKD) poses a significant health challenge for individuals with diabetes. At its initial stages, DKD often presents asymptomatically, and the standard for non-invasive diagnosis, the albumin-creatinine ratio (ACR), employs discrete categorizations (normal, microalbuminuria, macroalbuminuria) with limitations in sensitivity and specificity across diverse population cohorts. Single biomarker reliance further restricts the predictive value in clinical settings. Given the escalating prevalence of diabetes, o...
Source: Journal of Proteomics - April 4, 2024 Category: Biochemistry Authors: Jeremy Altman Shan Bai Sharad Purohit John White Dennis Steed Su Liu Diane Hopkins Jin-Xiong She Ashok Sharma Wenbo Zhi Source Type: research
Use of data-independent acquisition mass spectrometry to identify an objective serum indicator of the need for osteoporotic therapeutic intervention
This study aimed to identify objective serum indicators of the need for therapeutic intervention in individuals at risk of osteoporotic fracture. Comprehensive proteome analyses of serum collected from patients with osteoporosis but no fractures, patients with osteopenia and fragility fractures, and controls were performed by data-independent acquisition mass spectrometry. Collective evaluation of the proteome analysis data and ELISA-based assays identified serum ECM1 as a potential objective marker of the risk of fragility fractures in patients with osteoporosis or osteopenia. The findings are an important step toward the...
Source: Journal of Proteomics - April 4, 2024 Category: Biochemistry Authors: Yusuke Nakai Ken Kumagai Yoko Ino Tomoko Akiyama Kayano Moriyama Yuriko Takeda Kenji Egashira Takashi Ohira Akihide Ryo Tomoyuki Saito Yutaka Inaba Hisashi Hirano Yayoi Kimura Source Type: research
A candidate panel of eight urinary proteins shows potential of early diagnosis and risk assessment for diabetic kidney disease in type 1 diabetes
J Proteomics. 2024 Apr 2:105167. doi: 10.1016/j.jprot.2024.105167. Online ahead of print.ABSTRACTDiabetic kidney disease (DKD) poses a significant health challenge for individuals with diabetes. At its initial stages, DKD often presents asymptomatically, and the standard for non-invasive diagnosis, the albumin-creatinine ratio (ACR), employs discrete categorizations (normal, microalbuminuria, macroalbuminuria) with limitations in sensitivity and specificity across diverse population cohorts. Single biomarker reliance further restricts the predictive value in clinical settings. Given the escalating prevalence of diabetes, o...
Source: Journal of Proteomics - April 4, 2024 Category: Biochemistry Authors: Jeremy Altman Shan Bai Sharad Purohit John White Dennis Steed Su Liu Diane Hopkins Jin-Xiong She Ashok Sharma Wenbo Zhi Source Type: research
Use of data-independent acquisition mass spectrometry to identify an objective serum indicator of the need for osteoporotic therapeutic intervention
This study aimed to identify objective serum indicators of the need for therapeutic intervention in individuals at risk of osteoporotic fracture. Comprehensive proteome analyses of serum collected from patients with osteoporosis but no fractures, patients with osteopenia and fragility fractures, and controls were performed by data-independent acquisition mass spectrometry. Collective evaluation of the proteome analysis data and ELISA-based assays identified serum ECM1 as a potential objective marker of the risk of fragility fractures in patients with osteoporosis or osteopenia. The findings are an important step toward the...
Source: Journal of Proteomics - April 4, 2024 Category: Biochemistry Authors: Yusuke Nakai Ken Kumagai Yoko Ino Tomoko Akiyama Kayano Moriyama Yuriko Takeda Kenji Egashira Takashi Ohira Akihide Ryo Tomoyuki Saito Yutaka Inaba Hisashi Hirano Yayoi Kimura Source Type: research
A candidate panel of eight urinary proteins shows potential of early diagnosis and risk assessment for diabetic kidney disease in type 1 diabetes
J Proteomics. 2024 Apr 2:105167. doi: 10.1016/j.jprot.2024.105167. Online ahead of print.ABSTRACTDiabetic kidney disease (DKD) poses a significant health challenge for individuals with diabetes. At its initial stages, DKD often presents asymptomatically, and the standard for non-invasive diagnosis, the albumin-creatinine ratio (ACR), employs discrete categorizations (normal, microalbuminuria, macroalbuminuria) with limitations in sensitivity and specificity across diverse population cohorts. Single biomarker reliance further restricts the predictive value in clinical settings. Given the escalating prevalence of diabetes, o...
Source: Journal of Proteomics - April 4, 2024 Category: Biochemistry Authors: Jeremy Altman Shan Bai Sharad Purohit John White Dennis Steed Su Liu Diane Hopkins Jin-Xiong She Ashok Sharma Wenbo Zhi Source Type: research
Use of data-independent acquisition mass spectrometry to identify an objective serum indicator of the need for osteoporotic therapeutic intervention
This study aimed to identify objective serum indicators of the need for therapeutic intervention in individuals at risk of osteoporotic fracture. Comprehensive proteome analyses of serum collected from patients with osteoporosis but no fractures, patients with osteopenia and fragility fractures, and controls were performed by data-independent acquisition mass spectrometry. Collective evaluation of the proteome analysis data and ELISA-based assays identified serum ECM1 as a potential objective marker of the risk of fragility fractures in patients with osteoporosis or osteopenia. The findings are an important step toward the...
Source: Journal of Proteomics - April 4, 2024 Category: Biochemistry Authors: Yusuke Nakai Ken Kumagai Yoko Ino Tomoko Akiyama Kayano Moriyama Yuriko Takeda Kenji Egashira Takashi Ohira Akihide Ryo Tomoyuki Saito Yutaka Inaba Hisashi Hirano Yayoi Kimura Source Type: research
