S-layer is a key element in metabolic response and entry into the stationary phase in Bacillus cereus AH187
In this study, we used high-throughput proteomics to investigate how S-layer synthesis affects the allocation of cellular resources in the emetic B. cereus strain AH187. Our results demonstrate that the synthesis of the S-layer plays a crucial role in the pathogen's ability to thrive under stationary growth phase conditions by modulating the stress response, thereby promoting its lifestyle as an emetic pathogen. We conclude that the synthesis of the S-layer is a critical adaptation for emetic B. cereus to successfully colonize specific niches.PMID:37730087 | DOI:10.1016/j.jprot.2023.105007 (Source: Journal of Proteomics)
Source: Journal of Proteomics - September 20, 2023 Category: Biochemistry Authors: C écile Boutonnet Christian Ginies B éatrice Alpha-Bazin Jean Armengaud Alice Ch âteau Catherine Duport Source Type: research
S-layer is a key element in metabolic response and entry into the stationary phase in Bacillus cereus AH187
In this study, we used high-throughput proteomics to investigate how S-layer synthesis affects the allocation of cellular resources in the emetic B. cereus strain AH187. Our results demonstrate that the synthesis of the S-layer plays a crucial role in the pathogen's ability to thrive under stationary growth phase conditions by modulating the stress response, thereby promoting its lifestyle as an emetic pathogen. We conclude that the synthesis of the S-layer is a critical adaptation for emetic B. cereus to successfully colonize specific niches.PMID:37730087 | DOI:10.1016/j.jprot.2023.105007 (Source: Journal of Proteomics)
Source: Journal of Proteomics - September 20, 2023 Category: Biochemistry Authors: C écile Boutonnet Christian Ginies B éatrice Alpha-Bazin Jean Armengaud Alice Ch âteau Catherine Duport Source Type: research
S-layer is a key element in metabolic response and entry into the stationary phase in Bacillus cereus AH187
In this study, we used high-throughput proteomics to investigate how S-layer synthesis affects the allocation of cellular resources in the emetic B. cereus strain AH187. Our results demonstrate that the synthesis of the S-layer plays a crucial role in the pathogen's ability to thrive under stationary growth phase conditions by modulating the stress response, thereby promoting its lifestyle as an emetic pathogen. We conclude that the synthesis of the S-layer is a critical adaptation for emetic B. cereus to successfully colonize specific niches.PMID:37730087 | DOI:10.1016/j.jprot.2023.105007 (Source: Journal of Proteomics)
Source: Journal of Proteomics - September 20, 2023 Category: Biochemistry Authors: C écile Boutonnet Christian Ginies B éatrice Alpha-Bazin Jean Armengaud Alice Ch âteau Catherine Duport Source Type: research
Interactive effects of Pseudomonas entomophila strain 23S and Clavibacter michiganensis subsp. michiganensis on proteome and anti-Cmm compound production
In this study, the interactions between pure cultures of P. entomophila 23S and Cmm were investigated. First, the population dynamics of each bacterium during the interaction was determined using the selective media. Second, the amount of anti-Cmm compound produced by P. entomophila 23S in the presence of Cmm was quantified using HPLC. Lastly, a label-free shotgun proteomics study of P. entomophila 23S, Cmm, and a co-culture was conducted to understand the effects of the interaction of each bacterium at the proteomic level. Compared with the pure culture grown, the total number of proteins decreased in the interaction for ...
Source: Journal of Proteomics - September 17, 2023 Category: Biochemistry Authors: Yoko Takishita Sowmyalakshmi Subramanian Alfred Souleimanov Donald L Smith Source Type: research
Interactive effects of Pseudomonas entomophila strain 23S and Clavibacter michiganensis subsp. michiganensis on proteome and anti-Cmm compound production
In this study, the interactions between pure cultures of P. entomophila 23S and Cmm were investigated. First, the population dynamics of each bacterium during the interaction was determined using the selective media. Second, the amount of anti-Cmm compound produced by P. entomophila 23S in the presence of Cmm was quantified using HPLC. Lastly, a label-free shotgun proteomics study of P. entomophila 23S, Cmm, and a co-culture was conducted to understand the effects of the interaction of each bacterium at the proteomic level. Compared with the pure culture grown, the total number of proteins decreased in the interaction for ...
Source: Journal of Proteomics - September 17, 2023 Category: Biochemistry Authors: Yoko Takishita Sowmyalakshmi Subramanian Alfred Souleimanov Donald L Smith Source Type: research
Interactive effects of Pseudomonas entomophila strain 23S and Clavibacter michiganensis subsp. michiganensis on proteome and anti-Cmm compound production
In this study, the interactions between pure cultures of P. entomophila 23S and Cmm were investigated. First, the population dynamics of each bacterium during the interaction was determined using the selective media. Second, the amount of anti-Cmm compound produced by P. entomophila 23S in the presence of Cmm was quantified using HPLC. Lastly, a label-free shotgun proteomics study of P. entomophila 23S, Cmm, and a co-culture was conducted to understand the effects of the interaction of each bacterium at the proteomic level. Compared with the pure culture grown, the total number of proteins decreased in the interaction for ...
Source: Journal of Proteomics - September 17, 2023 Category: Biochemistry Authors: Yoko Takishita Sowmyalakshmi Subramanian Alfred Souleimanov Donald L Smith Source Type: research
DIA-based analysis of the menstrual blood proteome identifies association between CXCL5 and IL1RN and endometriosis
This study contributes to the identification of putative endometriosis biomarkers from non-invasive samples and lays the groundwork for future research into the roles of CXCL5 and IL1RN in the pathogenesis of endometriosis.PMID:37657716 | DOI:10.1016/j.jprot.2023.104995 (Source: Journal of Proteomics)
Source: Journal of Proteomics - September 1, 2023 Category: Biochemistry Authors: Sifan Ji Yuan Liu Li Yan Yiqin Zhang Yamei Li Qian Zhu Wei Xia Shunna Ge Jian Zhang Source Type: research
In-depth characterization of the sarcoplasmic muscle proteome changes in lambs fed with hazelnut skin by-products: Relationships with meat color
This study investigated the effect of agro-industrial hazelnut skin by-products supplementation on lamb meat color variation and the changes in the sarcoplasmic muscle proteome during post-mortem storage (0, 4 and 7 days). Gel-based proteomics and bioinformatics approaches were applied to better understand the potential role of feeding strategies in modulating the mechanisms underpinning meat discoloration and post-mortem changes during storage. Therefore, twenty-two Valle del Belice male lambs were randomly assigned to two dietary treatments: control (C), lambs fed with maize-barley diet, and hazelnut skin (H), lambs fed ...
Source: Journal of Proteomics - September 1, 2023 Category: Biochemistry Authors: Antonella Della Malva Mohammed Gagaoua Antonella Santillo Martina di Corcia Antonio Natalello Agostino Sevi Marzia Albenzio Source Type: research
Proteomic analysis of murine kidney proximal tubule sub-segment derived cell lines reveals preferences in mitochondrial pathway activity
This study analyzed three immortalized murine renal cell lines (PT S1, S2, and S3 segments) for protein content and compared them to a murine fibroblast cell line. All three proximal tubule cell lines generate ATP predominantly by oxidative phosphorylation while the fibroblast cell line is glycolytic. The proteomic data demonstrates that the most significant difference in proteomic signatures between the cell lines are proteins known to be localized in the nucleus followed by mitochondrial proteins. Mitochondrial metabolic substrate utilization assays were performed using the proximal tubule cell lines to determine substra...
Source: Journal of Proteomics - September 1, 2023 Category: Biochemistry Authors: Ricardo Melo Ferreira Rita de Almeida Clayton Culp Frank Witzmann Mu Wang Rajesh Kher Glenn T Nagami Rodrigo Mohallem Chaylen Jade Andolino Uma K Aryal Michael T Eadon Robert L Bacallao Source Type: research
Proteomic profiling analysis of human endometrium in women with unexplained recurrent spontaneous abortion
This study aimed to reveal the pathogenesis of URSA by analyzing the differential protein expression profiles in the decidual tissue of patients with recurrent abortion compared to those with normal pregnancy. Morphological analysis revealed abnormal decidualization of endometrial tissue in patients with URSA. Quantitative proteomics analysis showed that a total of 146 differentially expressed proteins were identified between the two groups, among which 95 proteins were downregulated and 51 proteins were upregulated. Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways revealed that the prot...
Source: Journal of Proteomics - September 1, 2023 Category: Biochemistry Authors: Lei Zhang Qian Li Yan Su Xinyuan Zhang Jialin Qu Dan Liao Qin Zou Hua Zou Xiaoli Liu Chunli Li Junlin He Source Type: research
DIA-based analysis of the menstrual blood proteome identifies association between CXCL5 and IL1RN and endometriosis
This study contributes to the identification of putative endometriosis biomarkers from non-invasive samples and lays the groundwork for future research into the roles of CXCL5 and IL1RN in the pathogenesis of endometriosis.PMID:37657716 | DOI:10.1016/j.jprot.2023.104995 (Source: Journal of Proteomics)
Source: Journal of Proteomics - September 1, 2023 Category: Biochemistry Authors: Sifan Ji Yuan Liu Li Yan Yiqin Zhang Yamei Li Qian Zhu Wei Xia Shunna Ge Jian Zhang Source Type: research
In-depth characterization of the sarcoplasmic muscle proteome changes in lambs fed with hazelnut skin by-products: Relationships with meat color
This study investigated the effect of agro-industrial hazelnut skin by-products supplementation on lamb meat color variation and the changes in the sarcoplasmic muscle proteome during post-mortem storage (0, 4 and 7 days). Gel-based proteomics and bioinformatics approaches were applied to better understand the potential role of feeding strategies in modulating the mechanisms underpinning meat discoloration and post-mortem changes during storage. Therefore, twenty-two Valle del Belice male lambs were randomly assigned to two dietary treatments: control (C), lambs fed with maize-barley diet, and hazelnut skin (H), lambs fed ...
Source: Journal of Proteomics - September 1, 2023 Category: Biochemistry Authors: Antonella Della Malva Mohammed Gagaoua Antonella Santillo Martina di Corcia Antonio Natalello Agostino Sevi Marzia Albenzio Source Type: research
Proteomic analysis of murine kidney proximal tubule sub-segment derived cell lines reveals preferences in mitochondrial pathway activity
This study analyzed three immortalized murine renal cell lines (PT S1, S2, and S3 segments) for protein content and compared them to a murine fibroblast cell line. All three proximal tubule cell lines generate ATP predominantly by oxidative phosphorylation while the fibroblast cell line is glycolytic. The proteomic data demonstrates that the most significant difference in proteomic signatures between the cell lines are proteins known to be localized in the nucleus followed by mitochondrial proteins. Mitochondrial metabolic substrate utilization assays were performed using the proximal tubule cell lines to determine substra...
Source: Journal of Proteomics - September 1, 2023 Category: Biochemistry Authors: Ricardo Melo Ferreira Rita de Almeida Clayton Culp Frank Witzmann Mu Wang Rajesh Kher Glenn T Nagami Rodrigo Mohallem Chaylen Jade Andolino Uma K Aryal Michael T Eadon Robert L Bacallao Source Type: research
Proteomic profiling analysis of human endometrium in women with unexplained recurrent spontaneous abortion
This study aimed to reveal the pathogenesis of URSA by analyzing the differential protein expression profiles in the decidual tissue of patients with recurrent abortion compared to those with normal pregnancy. Morphological analysis revealed abnormal decidualization of endometrial tissue in patients with URSA. Quantitative proteomics analysis showed that a total of 146 differentially expressed proteins were identified between the two groups, among which 95 proteins were downregulated and 51 proteins were upregulated. Gene Ontology (GO) terms and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways revealed that the prot...
Source: Journal of Proteomics - September 1, 2023 Category: Biochemistry Authors: Lei Zhang Qian Li Yan Su Xinyuan Zhang Jialin Qu Dan Liao Qin Zou Hua Zou Xiaoli Liu Chunli Li Junlin He Source Type: research
DIA-based analysis of the menstrual blood proteome identifies association between CXCL5 and IL1RN and endometriosis
This study contributes to the identification of putative endometriosis biomarkers from non-invasive samples and lays the groundwork for future research into the roles of CXCL5 and IL1RN in the pathogenesis of endometriosis.PMID:37657716 | DOI:10.1016/j.jprot.2023.104995 (Source: Journal of Proteomics)
Source: Journal of Proteomics - September 1, 2023 Category: Biochemistry Authors: Sifan Ji Yuan Liu Li Yan Yiqin Zhang Yamei Li Qian Zhu Wei Xia Shunna Ge Jian Zhang Source Type: research
