SRSF1 inhibits ferroptosis and reduces cisplatin chemosensitivity of triple-negative breast cancer cells through the circSEPT9/GCH1 axis
This study aimed to explore the functional mechanism of SR-rich splicing factor 1 (SRSF1) in DDP chemosensitivity of TNBC cells. Levels of SRSF1, circular RNA septin 9 (circSEPT9), and GTP cyclohydrolase-1 (GCH1) in TNBC cells, DDP-resistant cells, and normal cells were determined. Cell viability, half-maximal inhibitory concentration (IC50) value, and proliferation were evaluated. Ferroptosis was determined by assay kits (ferric ion/ROS/MDA/GSH) and Western blot assay (SLC7A11/ACSL4). The genetic binding was analyzed by RNA immunoprecipitation and RNA pull-down assays. SRSF1, circSEPT9, and GCH1 were upregulated in TNBC c...
Source: Journal of Proteomics - December 1, 2023 Category: Biochemistry Authors: Xiang Song Xinzhao Wang Xiqi Chen Zhiyong Yu Yongkun Zhou Source Type: research
SRSF1 inhibits ferroptosis and reduces cisplatin chemosensitivity of triple-negative breast cancer cells through the circSEPT9/GCH1 axis
This study aimed to explore the functional mechanism of SR-rich splicing factor 1 (SRSF1) in DDP chemosensitivity of TNBC cells. Levels of SRSF1, circular RNA septin 9 (circSEPT9), and GTP cyclohydrolase-1 (GCH1) in TNBC cells, DDP-resistant cells, and normal cells were determined. Cell viability, half-maximal inhibitory concentration (IC50) value, and proliferation were evaluated. Ferroptosis was determined by assay kits (ferric ion/ROS/MDA/GSH) and Western blot assay (SLC7A11/ACSL4). The genetic binding was analyzed by RNA immunoprecipitation and RNA pull-down assays. SRSF1, circSEPT9, and GCH1 were upregulated in TNBC c...
Source: Journal of Proteomics - December 1, 2023 Category: Biochemistry Authors: Xiang Song Xinzhao Wang Xiqi Chen Zhiyong Yu Yongkun Zhou Source Type: research
Exoskeleton protein repertoires in decapod crustaceans revealed distinct biomineralization evolution with molluscs
This study filled such a gap by using proteomic analysis of matrix proteins from four decapod crustacean exoskeletons. Many novel proteins were discovered which enabled a solid comparison with those of molluscs. By comparison, we proposed that crustaceans evolved novel proteins to form mineralized exoskeletons while sharing some proteins with those of molluscs. This is useful for us to understand the evolution of two major biomineralized phylum.PMID:37981007 | DOI:10.1016/j.jprot.2023.105046 (Source: Journal of Proteomics)
Source: Journal of Proteomics - November 19, 2023 Category: Biochemistry Authors: Chuang Liu Wenjing Zhang Qianli Dong Haipeng Liu Source Type: research
Proteomic investigation reveals the role of bacterial laccase from Bacillus pumilus in oxidative stress defense
In this study, the differentially expressed proteins were analyzed using a TMT-labeling proteomic approach when B. pumilus was treated with methyl methanesulfonate (MMS). Reactive oxygen species induced by MMS activated the secondary metabolites biosynthesis, transport, and catabolism in B. pumilus, including laccase overexpression. Moreover, the simultaneously up-regulated YcnJ and GabP may benefit the synthesis and the stability of laccase, then improve the antioxidative property of B. pumilus against environmental stress. Our findings advance the understanding of the adaptive mechanism of B. pumilus to environmental con...
Source: Journal of Proteomics - November 19, 2023 Category: Biochemistry Authors: Bianxia Li Zhuocheng Jin Fan Yang Huanan Li Jiashu Liu Zhengbing Jiang Source Type: research
Toxin metalloproteinases exert a dominant influence on pro-inflammatory response and anti-inflammatory regulation in jellyfish sting dermatitis
In this study, we conducted a differential analysis of a dermatitis mouse model induced by jellyfish Nemopilema nomurai venom (NnNV) samples with varying levels of metalloproteinase activity. Through skin tissue proteomics and serum metabolomics, the predominant influence of toxin metalloproteinase activity on inflammatory response was revealed, and the signal pathway involved in its regulation was identified. In skin tissues, many membrane proteins were significantly down-regulated, which might cause tissue damage. The expression of pro-inflammatory factors was mainly regulated by PI3K-Akt signaling pathway. In serum, man...
Source: Journal of Proteomics - November 19, 2023 Category: Biochemistry Authors: Chunlin Yu Xiujing Yin Aoyu Li Rongfeng Li Huahua Yu Ronge Xing Song Liu Pengcheng Li Source Type: research
Exoskeleton protein repertoires in decapod crustaceans revealed distinct biomineralization evolution with molluscs
This study filled such a gap by using proteomic analysis of matrix proteins from four decapod crustacean exoskeletons. Many novel proteins were discovered which enabled a solid comparison with those of molluscs. By comparison, we proposed that crustaceans evolved novel proteins to form mineralized exoskeletons while sharing some proteins with those of molluscs. This is useful for us to understand the evolution of two major biomineralized phylum.PMID:37981007 | DOI:10.1016/j.jprot.2023.105046 (Source: Journal of Proteomics)
Source: Journal of Proteomics - November 19, 2023 Category: Biochemistry Authors: Chuang Liu Wenjing Zhang Qianli Dong Haipeng Liu Source Type: research
Proteomic investigation reveals the role of bacterial laccase from Bacillus pumilus in oxidative stress defense
In this study, the differentially expressed proteins were analyzed using a TMT-labeling proteomic approach when B. pumilus was treated with methyl methanesulfonate (MMS). Reactive oxygen species induced by MMS activated the secondary metabolites biosynthesis, transport, and catabolism in B. pumilus, including laccase overexpression. Moreover, the simultaneously up-regulated YcnJ and GabP may benefit the synthesis and the stability of laccase, then improve the antioxidative property of B. pumilus against environmental stress. Our findings advance the understanding of the adaptive mechanism of B. pumilus to environmental con...
Source: Journal of Proteomics - November 19, 2023 Category: Biochemistry Authors: Bianxia Li Zhuocheng Jin Fan Yang Huanan Li Jiashu Liu Zhengbing Jiang Source Type: research
Toxin metalloproteinases exert a dominant influence on pro-inflammatory response and anti-inflammatory regulation in jellyfish sting dermatitis
In this study, we conducted a differential analysis of a dermatitis mouse model induced by jellyfish Nemopilema nomurai venom (NnNV) samples with varying levels of metalloproteinase activity. Through skin tissue proteomics and serum metabolomics, the predominant influence of toxin metalloproteinase activity on inflammatory response was revealed, and the signal pathway involved in its regulation was identified. In skin tissues, many membrane proteins were significantly down-regulated, which might cause tissue damage. The expression of pro-inflammatory factors was mainly regulated by PI3K-Akt signaling pathway. In serum, man...
Source: Journal of Proteomics - November 19, 2023 Category: Biochemistry Authors: Chunlin Yu Xiujing Yin Aoyu Li Rongfeng Li Huahua Yu Ronge Xing Song Liu Pengcheng Li Source Type: research
Exoskeleton protein repertoires in decapod crustaceans revealed distinct biomineralization evolution with molluscs
This study filled such a gap by using proteomic analysis of matrix proteins from four decapod crustacean exoskeletons. Many novel proteins were discovered which enabled a solid comparison with those of molluscs. By comparison, we proposed that crustaceans evolved novel proteins to form mineralized exoskeletons while sharing some proteins with those of molluscs. This is useful for us to understand the evolution of two major biomineralized phylum.PMID:37981007 | DOI:10.1016/j.jprot.2023.105046 (Source: Journal of Proteomics)
Source: Journal of Proteomics - November 19, 2023 Category: Biochemistry Authors: Chuang Liu Wenjing Zhang Qianli Dong Haipeng Liu Source Type: research
Proteomic investigation reveals the role of bacterial laccase from Bacillus pumilus in oxidative stress defense
In this study, the differentially expressed proteins were analyzed using a TMT-labeling proteomic approach when B. pumilus was treated with methyl methanesulfonate (MMS). Reactive oxygen species induced by MMS activated the secondary metabolites biosynthesis, transport, and catabolism in B. pumilus, including laccase overexpression. Moreover, the simultaneously up-regulated YcnJ and GabP may benefit the synthesis and the stability of laccase, then improve the antioxidative property of B. pumilus against environmental stress. Our findings advance the understanding of the adaptive mechanism of B. pumilus to environmental con...
Source: Journal of Proteomics - November 19, 2023 Category: Biochemistry Authors: Bianxia Li Zhuocheng Jin Fan Yang Huanan Li Jiashu Liu Zhengbing Jiang Source Type: research
Toxin metalloproteinases exert a dominant influence on pro-inflammatory response and anti-inflammatory regulation in jellyfish sting dermatitis
In this study, we conducted a differential analysis of a dermatitis mouse model induced by jellyfish Nemopilema nomurai venom (NnNV) samples with varying levels of metalloproteinase activity. Through skin tissue proteomics and serum metabolomics, the predominant influence of toxin metalloproteinase activity on inflammatory response was revealed, and the signal pathway involved in its regulation was identified. In skin tissues, many membrane proteins were significantly down-regulated, which might cause tissue damage. The expression of pro-inflammatory factors was mainly regulated by PI3K-Akt signaling pathway. In serum, man...
Source: Journal of Proteomics - November 19, 2023 Category: Biochemistry Authors: Chunlin Yu Xiujing Yin Aoyu Li Rongfeng Li Huahua Yu Ronge Xing Song Liu Pengcheng Li Source Type: research
Exoskeleton protein repertoires in decapod crustaceans revealed distinct biomineralization evolution with molluscs
This study filled such a gap by using proteomic analysis of matrix proteins from four decapod crustacean exoskeletons. Many novel proteins were discovered which enabled a solid comparison with those of molluscs. By comparison, we proposed that crustaceans evolved novel proteins to form mineralized exoskeletons while sharing some proteins with those of molluscs. This is useful for us to understand the evolution of two major biomineralized phylum.PMID:37981007 | DOI:10.1016/j.jprot.2023.105046 (Source: Journal of Proteomics)
Source: Journal of Proteomics - November 19, 2023 Category: Biochemistry Authors: Chuang Liu Wenjing Zhang Qianli Dong Haipeng Liu Source Type: research
Proteomic investigation reveals the role of bacterial laccase from Bacillus pumilus in oxidative stress defense
In this study, the differentially expressed proteins were analyzed using a TMT-labeling proteomic approach when B. pumilus was treated with methyl methanesulfonate (MMS). Reactive oxygen species induced by MMS activated the secondary metabolites biosynthesis, transport, and catabolism in B. pumilus, including laccase overexpression. Moreover, the simultaneously up-regulated YcnJ and GabP may benefit the synthesis and the stability of laccase, then improve the antioxidative property of B. pumilus against environmental stress. Our findings advance the understanding of the adaptive mechanism of B. pumilus to environmental con...
Source: Journal of Proteomics - November 19, 2023 Category: Biochemistry Authors: Bianxia Li Zhuocheng Jin Fan Yang Huanan Li Jiashu Liu Zhengbing Jiang Source Type: research
Toxin metalloproteinases exert a dominant influence on pro-inflammatory response and anti-inflammatory regulation in jellyfish sting dermatitis
In this study, we conducted a differential analysis of a dermatitis mouse model induced by jellyfish Nemopilema nomurai venom (NnNV) samples with varying levels of metalloproteinase activity. Through skin tissue proteomics and serum metabolomics, the predominant influence of toxin metalloproteinase activity on inflammatory response was revealed, and the signal pathway involved in its regulation was identified. In skin tissues, many membrane proteins were significantly down-regulated, which might cause tissue damage. The expression of pro-inflammatory factors was mainly regulated by PI3K-Akt signaling pathway. In serum, man...
Source: Journal of Proteomics - November 19, 2023 Category: Biochemistry Authors: Chunlin Yu Xiujing Yin Aoyu Li Rongfeng Li Huahua Yu Ronge Xing Song Liu Pengcheng Li Source Type: research
Exoskeleton protein repertoires in decapod crustaceans revealed distinct biomineralization evolution with molluscs
This study filled such a gap by using proteomic analysis of matrix proteins from four decapod crustacean exoskeletons. Many novel proteins were discovered which enabled a solid comparison with those of molluscs. By comparison, we proposed that crustaceans evolved novel proteins to form mineralized exoskeletons while sharing some proteins with those of molluscs. This is useful for us to understand the evolution of two major biomineralized phylum.PMID:37981007 | DOI:10.1016/j.jprot.2023.105046 (Source: Journal of Proteomics)
Source: Journal of Proteomics - November 19, 2023 Category: Biochemistry Authors: Chuang Liu Wenjing Zhang Qianli Dong Haipeng Liu Source Type: research
