Simplified COI barcoding of blow, flesh, and scuttle flies encountered in medicolegal investigations
We describe here a simplified DNA barcoding method for identifying relevant species that may be implemented by forensic genetics laboratories. A cytochrome oxidase (COI) fragment is analyzed after PCR amplification with a single primer set. The method is effective for many species commonly encountered in death investigations in the USA: members of blowfly generaCalliphora,Chrysomya,Cochliomyia,Lucilia, andPhormia; members of the flesh fly generaBlaesoxipha,Oxysarcodexia,Ravinia, andSarcophaga; and the scuttle flyMegaselia scalaris. We tested the method on specimens with verified identifications and used it to build a colle...
Source: Forensic Science, Medicine, and Pathology - May 24, 2023 Category: Forensic Medicine Source Type: research

Cameriere ’s open apices methodology for dental age estimation in children: a scoping review from a Latin American perspective
AbstractDental age estimation (DAE) is key to establishing an individual ’s identity, and this is relevant in minors. A widely used method for DAE in children is Cameriere’s open apices (CAM). Despite its wide dissemination, there are no clear reports of its use in Latin American populations. A scoping review was conducted using a search strategy in the PubMed/MEDLIN E database, Web of Science, and a complementary manual search. Only papers using CAM or its regression equation models for evaluating Latin American populations were included. Ten studies published between 2007 and 2020 responded to the search objective. B...
Source: Forensic Science, Medicine, and Pathology - May 24, 2023 Category: Forensic Medicine Source Type: research

Acute subdural hematoma caused by rupture of a mycotic aneurysm due to meningitis associated with infectious endocarditis: comparison of autopsy findings with postmortem computed tomography
AbstractForensic pathologists often encounter cases of acute subdural hematoma (SDH) due to trauma, whereas those attributable to endogenous causes are rare. Here, we report a case of the latter type in a 42-year-old man who was found dead at home after several months of fever and malaise. Postmortem computed tomography (PMCT) and autopsy were undertaken to clarify the cause of death. PMCT images revealed a fatal SDH and a localized hyper-density area in the right parietal lobe; macroscopic and microscopic examinations revealed SDH due to rupture of a mycotic aneurysm (MA) associated with meningitis. The PMCT images also i...
Source: Forensic Science, Medicine, and Pathology - May 24, 2023 Category: Forensic Medicine Source Type: research

The transversoclasiotome: a novel instrument for examining the vertebral artery
AbstractOpening the foramen transversarium of the cervical vertebrae is necessary for accessing the vertebral vessels. There are no specialist tools for cutting the anterior lamina of the transverse processes, and alternatives lead to questionable results. A novel tool, the transversoclasiotome, is described and tested.  The literature and patent databases were systematically reviewed. A blueprint of the transversoclasiotome was created, and the prototype was tested through autopsy on ten fresh-frozen cadavers within our Body Donation Program. The transversoclasiotome consists of two delicate branches mounted as a scisso...
Source: Forensic Science, Medicine, and Pathology - May 24, 2023 Category: Forensic Medicine Source Type: research

Four cases of fatal acute arsenic poisoning: histopathology, toxicology, and new trends
This study summarizes the histopathological features of arsenic poisoning and presents data on arsenic distrib ution. Arsenic concentrations in the liver and kidneys can increase the reliability of identifying arsenic poisoning. Furthermore, in traditional Chinese medicine-related deaths, arsenic poisoning needs more attention. (Source: Forensic Science, Medicine, and Pathology)
Source: Forensic Science, Medicine, and Pathology - May 24, 2023 Category: Forensic Medicine Source Type: research