A harmful MYH11 variant detected in a family with thoracic aortic dissection and patent ductus arteriosus
We present this familial case to stress the necessity of postmortem genetic testing in such cases among forensic practices. Identifying those culprit gene variants can direct effective genetic counseling and personalized health management in family members (especially first-degree relatives) with high-risk genotypes. (Source: Forensic Science, Medicine, and Pathology)
Source: Forensic Science, Medicine, and Pathology - June 12, 2023 Category: Forensic Medicine Source Type: research

Acknowledgements
(Source: Forensic Science, Medicine, and Pathology)
Source: Forensic Science, Medicine, and Pathology - June 9, 2023 Category: Forensic Medicine Source Type: research

Multiorgan immunohistochemical endothelial expression of E-selectin in a forensic case of sepsis
This report describes the gross pathological findings obtained from the autopsy of a 48-year-old woman with Crohn ’s disease after surgical intervention. Macroscopically, we found intestinal perforation and signs of peritonitis. Histologically, the pulmonary/bronchial arteries were lined with E-selectin (CD 62E)-positive endothelial cells, which are an established postmortem histological marker of sepsis. We extended our investigations to the cerebral cortex and subcortical medullary layer. The endothelium of the cortical vessels and those in the cerebral medullary layer were likewise immunopositive for E-selectin. Furth...
Source: Forensic Science, Medicine, and Pathology - June 8, 2023 Category: Forensic Medicine Source Type: research

Prediction of exophthalmos by body mass index for craniofacial reconstruction: consequences for cold cases
The objective of this study was to evaluate a non-population-dependent method of determining exophthalmos. The protrusion of the eyeball is known to vary with the contents of the orbital cavity based on bony orbital resorption or increased or decreased fat contents, as well as according to relative eyeball size. Of use are available statistics on body mass index, and this is discussed within the context of eyeball protrusion. A weak positive correlation (0.3263) between the body mass index of the country where the study originated, and the degree of exophthalmos was found. The results suggest that eyeball protrusion rates ...
Source: Forensic Science, Medicine, and Pathology - June 7, 2023 Category: Forensic Medicine Source Type: research

Age estimation from iliac auricular surface using Bayesian inference and principal component analysis: a CT-based study in an Indian population
AbstractAge estimation constitutes one of the pillars of human identification. The auricular surface of the ilium presents as a durable and robust structure within the human skeletal framework, capable of enabling accurate age estimation in older adults. Amongst different documented auricular age estimation methods, the Buckberry-Chamberlain method offers greater objectivity through its component-based approach. The present study aimed to test the applicability of the Buckberry-Chamberlain method in an Indian population through a CT-based examination of the auricular surface. CT scans of 435 participants undergoing CT exam...
Source: Forensic Science, Medicine, and Pathology - June 6, 2023 Category: Forensic Medicine Source Type: research

A pilot study investigating early postmortem interval of rats based on ambient temperature and postmortem interval-related metabolites in blood
In this study, gas chromatography –mass spectrometry (GC‒MS)‒based metabolomics was adopted to explore the changes in metabolites in the cardiac blood of suffocated rats at various ambient temperatures (5 °C, 15 °C, 25 °C, and 35 °C) from 0 to 24 h after death. Isoleucine, alanine, proline, valine, glycerol, glycerol phosphate, xanthine, and hypoxanthine were found to contribute to PMI in all temperature groups. Hypoxanthine and isoleucine were chosen to establish estimation models (equations) with an interpolation function using PMI as the dependent variable (f(x, y)), relative intensity as the independent ...
Source: Forensic Science, Medicine, and Pathology - June 2, 2023 Category: Forensic Medicine Source Type: research

Development of a multiplex panel with 36 insertion/deletion markers (InDel) for individual identification
In conclusion, the results showed that the 36-InDelplex panel is a reliable, sensitive, and accurate system that is suitable for human identification and population genetics purposes. (Source: Forensic Science, Medicine, and Pathology)
Source: Forensic Science, Medicine, and Pathology - June 2, 2023 Category: Forensic Medicine Source Type: research

Conventional HE staining combined with two special staining methods to identify antemortem stab wound in case with highly decomposed mutilated corpses
This study aims to identify an antemortem neck stab wound on a highly decomposed, headless and mutilated body by conventional hematoxylin-eosin (HE) staining combined with Ponceau-Victoria blue B staining (P-VB staining) and Masson staining. Specifically, a tissue sample was excised from the skin and muscle tissue at the junction of the normal and brownish discolored areas around the suspected stabbing tract of the left neck, in the upper and lower wound-clavicle-shoulder region. Conventional HE staining only provides a morphological and structural outline of the tissue, with both the injury hemorrhage and local connective...
Source: Forensic Science, Medicine, and Pathology - May 31, 2023 Category: Forensic Medicine Source Type: research

A beetle for prosecution: exogenous DNA detection from larval and adult gut of a Neotropical carrion beetle
This study evaluated whether the Neotropical carrion beetleOxelytrum discicolle (Silphidae) has the potential for the detection of exogenous DNA into the gut. The whole gut or the gut contents were extracted fromO. discicolle larvae and adult previously fed on pig carcass. The pig DNA recovery rate was 33.3% in larvae and 25% in adults, indicating that the carrion beetle ’s gut may be useful for DNA identification of ingested food. Samples with the whole gut or only gut contents showed the same DNA recovery rate. Exogenous DNA from the whole gut was recovered from samples stored in ethanol at -20 ºC for 11 days, showing...
Source: Forensic Science, Medicine, and Pathology - May 27, 2023 Category: Forensic Medicine Source Type: research

Cheiloscopy in sex estimation: a systematic review
This study aimed to conduct a systematic review to gather evidence to clarify if cheiloscopy can be used in sex estimation and identify the reasons behind the lack of consensus in the scientific community.  The systematic review was performed following the PRISMA guidelines. A bibliographic survey was conducted in PubMed, Scopus, and Web of Science databases, restricted to articles published between 2010 and 2020. Studies were selected according to eligibility criteria, and then the study data were c ollected. The risk of bias in each study was assessed and applied as additional inclusion or exclusion criteria. The resul...
Source: Forensic Science, Medicine, and Pathology - May 27, 2023 Category: Forensic Medicine Source Type: research

Cardiovascular-related death in infancy and childhood: a clinicopathological study of two referral institutions in England
AbstractTo review post-mortem findings among deaths presenting as sudden and/or unexpected deaths in two centers in the UK during a 16-year period in order to identify those related to cardiovascular conditions. The post-mortem databases of two tertiary referral institutions were searched, and all reports were reviewed. Histological features and results of ancillary investigations were noted. All cases of sudden and/or unexpected cardiac deaths (SCD) between 2003 and 2018 were identified. The study was PRISMA compliant and approved by clinical governance. 68/1129 cases of SCD (6.0%) were identified in one center and 83/753...
Source: Forensic Science, Medicine, and Pathology - May 26, 2023 Category: Forensic Medicine Source Type: research