Increased susceptibility to encephalitozoonosis associated with mixed Th1/Th2 profile and M1/M2 profile in mice immunosuppressed with cyclophosphamide
Exp Parasitol. 2023 Sep 2:108606. doi: 10.1016/j.exppara.2023.108606. Online ahead of print.ABSTRACTEncephalitozoon cuniculi is a unicellular, spore-forming, obligate intracellular eukaryote belonging to the phylum Microsporidia. It is known to infect mainly immunocompromised and immunocompetent mammals, including humans. The parasite-host relationship has been evaluated using both in vitro cell culturing and animal models. For example, Balb/c and C57BL/6 mouse strains have been used interchangeably, although the latter has been considered more susceptible due to the higher fungal load observed after infection. In the pres...
Source: Experimental Parasitology - September 4, 2023 Category: Parasitology Authors: Amanda Miyuki Hidifira Anuska Marcelino Alvares-Saraiva Elizabeth Christina Perez Diva Denelle Spadacci-Morena Ronalda Silva de Araujo Maria Anete Lallo Source Type: research
Pharmacokinetics of macrocyclic lactone endectocides in indigenous Zebu cattle and their insecticidal efficacy on Anopheles arabiensis
Exp Parasitol. 2023 Aug 31;253:108605. doi: 10.1016/j.exppara.2023.108605. Online ahead of print.ABSTRACTOutdoor biting, outdoor resting, and early evening biting of Anopheles arabiensis is a challenge in current malaria control and elimination efforts in Africa. Zooprophylaxis using livestock treated with macrocyclic lactones is a novel approach to control zoophilic vectors. Therefore, the present study aimed to investigate the pharmacokinetics and insecticidal efficacy of ivermectin (IVER), doramectin (DORA), and moxidectin (MOXI) subcutaneous (SC) formulations in treated calves. The study was conducted using indigenous ...
Source: Experimental Parasitology - September 2, 2023 Category: Parasitology Authors: Gemechu Zeleke Luc Duchateau Delenasaw Yewhalaw Sultan Suleman Mathias Devreese Source Type: research
Pharmacokinetics of macrocyclic lactone endectocides in indigenous Zebu cattle and their insecticidal efficacy on Anopheles arabiensis
Exp Parasitol. 2023 Aug 31:108605. doi: 10.1016/j.exppara.2023.108605. Online ahead of print.ABSTRACTOutdoor biting, outdoor resting, and early evening biting of Anopheles arabiensis is a challenge in current malaria control and elimination efforts in Africa. Zooprophylaxis using livestock treated with macrocyclic lactones is a novel approach to control zoophilic vectors. Therefore, the present study aimed to investigate the pharmacokinetics and insecticidal efficacy of ivermectin (IVER), doramectin (DORA), and moxidectin (MOXI) subcutaneous (SC) formulations in treated calves. The study was conducted using indigenous (Bos...
Source: Experimental Parasitology - September 2, 2023 Category: Parasitology Authors: Gemechu Zeleke Luc Duchateau Delenasaw Yewhalaw Sultan Suleman Mathias Devreese Source Type: research
Acaricidal activity of a 2-methoxy-clovan-9-ol rich fraction from Eugenia copacabanensis (Myrtaceae) extract on Rhipicephalus microplus (Acari: Ixodidae)
This study evaluated the acaricidal activity against R. microplus of the 2-methoxy-clovan-9-ol rich fraction obtained from Eugenia copacabanensis Kiaersk.leaves, a little known Myrtaceae species from the Brazilian Atlantic Forest. This fraction was obtained by maceration with methanol, partition with n-hexane and purification by normal-phase column chromatography. GC-MS, 1H and 13C NMR and IR analysis contributed to the identification of a major compound as the sesquiterpene 2-methoxy-clovan-9-ol, reported for the first time for the Myrtaceae family. The fraction was tested against R. microplus unfed larvae and engorged fe...
Source: Experimental Parasitology - August 27, 2023 Category: Parasitology Authors: V íctor de Carvalho Martins Paula Marchesini L ívia S Duque Liliana Princisval Fran ça Yasmim da Silva Ferreira Marcelo da Costa Souza Caio Monteiro Ronoel Luiz de Oliveira Godoy Mario Geraldo de Carvalho Source Type: research
Acaricidal activity of a 2-methoxy-clovan-9-ol rich fraction from Eugenia copacabanensis (Myrtaceae) extract on Rhipicephalus microplus (Acari: Ixodidae)
This study evaluated the acaricidal activity against R. microplus of the 2-methoxy-clovan-9-ol rich fraction obtained from Eugenia copacabanensis Kiaersk.leaves, a little known Myrtaceae species from the Brazilian Atlantic Forest. This fraction was obtained by maceration with methanol, partition with n-hexane and purification by normal-phase column chromatography. GC-MS, 1H and 13C NMR and IR analysis contributed to the identification of a major compound as the sesquiterpene 2-methoxy-clovan-9-ol, reported for the first time for the Myrtaceae family. The fraction was tested against R. microplus unfed larvae and engorged fe...
Source: Experimental Parasitology - August 27, 2023 Category: Parasitology Authors: V íctor de Carvalho Martins Paula Marchesini L ívia S Duque Liliana Princisval Fran ça Yasmim da Silva Ferreira Marcelo da Costa Souza Caio Monteiro Ronoel Luiz de Oliveira Godoy Mario Geraldo de Carvalho Source Type: research
Acaricidal activity of a 2-methoxy-clovan-9-ol rich fraction from Eugenia copacabanensis (Myrtaceae) extract on Rhipicephalus microplus (Acari: Ixodidae)
This study evaluated the acaricidal activity against R. microplus of the 2-methoxy-clovan-9-ol rich fraction obtained from Eugenia copacabanensis Kiaersk.leaves, a little known Myrtaceae species from the Brazilian Atlantic Forest. This fraction was obtained by maceration with methanol, partition with n-hexane and purification by normal-phase column chromatography. GC-MS, 1H and 13C NMR and IR analysis contributed to the identification of a major compound as the sesquiterpene 2-methoxy-clovan-9-ol, reported for the first time for the Myrtaceae family. The fraction was tested against R. microplus unfed larvae and engorged fe...
Source: Experimental Parasitology - August 27, 2023 Category: Parasitology Authors: V íctor de Carvalho Martins Paula Marchesini L ívia S Duque Liliana Princisval Fran ça Yasmim da Silva Ferreira Marcelo da Costa Souza Caio Monteiro Ronoel Luiz de Oliveira Godoy Mario Geraldo de Carvalho Source Type: research
Acaricidal activity of a 2-methoxy-clovan-9-ol rich fraction from Eugenia copacabanensis (Myrtaceae) extract on Rhipicephalus microplus (Acari: Ixodidae)
This study evaluated the acaricidal activity against R. microplus of the 2-methoxy-clovan-9-ol rich fraction obtained from Eugenia copacabanensis Kiaersk.leaves, a little known Myrtaceae species from the Brazilian Atlantic Forest. This fraction was obtained by maceration with methanol, partition with n-hexane and purification by normal-phase column chromatography. GC-MS, 1H and 13C NMR and IR analysis contributed to the identification of a major compound as the sesquiterpene 2-methoxy-clovan-9-ol, reported for the first time for the Myrtaceae family. The fraction was tested against R. microplus unfed larvae and engorged fe...
Source: Experimental Parasitology - August 27, 2023 Category: Parasitology Authors: V íctor de Carvalho Martins Paula Marchesini L ívia S Duque Liliana Princisval Fran ça Yasmim da Silva Ferreira Marcelo da Costa Souza Caio Monteiro Ronoel Luiz de Oliveira Godoy Mario Geraldo de Carvalho Source Type: research
Acaricidal activity of a 2-methoxy-clovan-9-ol rich fraction from Eugenia copacabanensis (Myrtaceae) extract on Rhipicephalus microplus (Acari: Ixodidae)
This study evaluated the acaricidal activity against R. microplus of the 2-methoxy-clovan-9-ol rich fraction obtained from Eugenia copacabanensis Kiaersk.leaves, a little known Myrtaceae species from the Brazilian Atlantic Forest. This fraction was obtained by maceration with methanol, partition with n-hexane and purification by normal-phase column chromatography. GC-MS, 1H and 13C NMR and IR analysis contributed to the identification of a major compound as the sesquiterpene 2-methoxy-clovan-9-ol, reported for the first time for the Myrtaceae family. The fraction was tested against R. microplus unfed larvae and engorged fe...
Source: Experimental Parasitology - August 27, 2023 Category: Parasitology Authors: V íctor de Carvalho Martins Paula Marchesini L ívia S Duque Liliana Princisval Fran ça Yasmim da Silva Ferreira Marcelo da Costa Souza Caio Monteiro Ronoel Luiz de Oliveira Godoy Mario Geraldo de Carvalho Source Type: research
Micro-RNA expression profile of BALB/c mouse glandular stomach in the early phase of Cryptosporidium muris infection
This study provides a new perspective on the miRNA regulatory mechanisms of cryptosporidiosis, which may help in the development of effective control strategies against this pathogen.PMID:37633513 | DOI:10.1016/j.exppara.2023.108603 (Source: Experimental Parasitology)
Source: Experimental Parasitology - August 26, 2023 Category: Parasitology Authors: Yankai Chang Songrui Li Luyang Wang Ke Wang Junqiang Li Xiaoying Li Fuchun Jian Rongjun Wang Sumei Zhang Longxian Zhang Source Type: research
Micro-RNA expression profile of BALB/c mouse glandular stomach in the early phase of Cryptosporidium muris infection
This study provides a new perspective on the miRNA regulatory mechanisms of cryptosporidiosis, which may help in the development of effective control strategies against this pathogen.PMID:37633513 | DOI:10.1016/j.exppara.2023.108603 (Source: Experimental Parasitology)
Source: Experimental Parasitology - August 26, 2023 Category: Parasitology Authors: Yankai Chang Songrui Li Luyang Wang Ke Wang Junqiang Li Xiaoying Li Fuchun Jian Rongjun Wang Sumei Zhang Longxian Zhang Source Type: research
Activity of compounds derived from benzofuroxan in Trichomonasvaginalis
This study evaluated the in vitro antiparasitic activity of these compounds in trophozoites of T. vaginalis and determined the mean inhibitory concentration (IC50), minimum inhibitory concentration (MIC), mortality curve, and cytotoxicity. The compounds were named EH1, EH2, EH3, and EA2 and tested in various concentrations: 100 to 15 μM (EH1 and EH2); 100 to 5 μM (EH3); and 100 to 25 μM (EA2), respectively. The greatest efficacy was observed in the highest concentrations in 24 h, with inhibition of approximately 100% of trophozoites. Compounds EH2 and EH3 had the lowest MIC: EH2 (35 μM) and EH3 (45 μM), with IC50 of 1...
Source: Experimental Parasitology - August 25, 2023 Category: Parasitology Authors: Marjorie Giacometi Jo ão Cândido Pilar Mayer Alexia Brauner de Mello Yan Wahast Islab ão Adriane Leites Strothmann Renata Nobre Fonseca Ângela Sena-Lopes Luciano Dornelles Sibele Borsuk Silvia de Oliveira H übner Camila Belmonte Oliveira Source Type: research
Activity of compounds derived from benzofuroxan in Trichomonasvaginalis
This study evaluated the in vitro antiparasitic activity of these compounds in trophozoites of T. vaginalis and determined the mean inhibitory concentration (IC50), minimum inhibitory concentration (MIC), mortality curve, and cytotoxicity. The compounds were named EH1, EH2, EH3, and EA2 and tested in various concentrations: 100 to 15 μM (EH1 and EH2); 100 to 5 μM (EH3); and 100 to 25 μM (EA2), respectively. The greatest efficacy was observed in the highest concentrations in 24 h, with inhibition of approximately 100% of trophozoites. Compounds EH2 and EH3 had the lowest MIC: EH2 (35 μM) and EH3 (45 μM), with IC50 of 1...
Source: Experimental Parasitology - August 25, 2023 Category: Parasitology Authors: Marjorie Giacometi Jo ão Cândido Pilar Mayer Alexia Brauner de Mello Yan Wahast Islab ão Adriane Leites Strothmann Renata Nobre Fonseca Ângela Sena-Lopes Luciano Dornelles Sibele Borsuk Silvia de Oliveira H übner Camila Belmonte Oliveira Source Type: research
Functional characterization of phospholipase B enzyme from Giardia lamblia
Exp Parasitol. 2023 Aug 22:108602. doi: 10.1016/j.exppara.2023.108602. Online ahead of print.ABSTRACTThe microaerotolarent amitochondriate protozoan Giardia lamblia causes Giardiasis and produces a unique enzyme called Phospholipase B (PLB) in contrast to higher eukaryotes. The enzyme is produced upon induction with oxidative (H2O2) stress, thus leading to prostaglandin E2 (PGE2) production. It exists in dimeric form, and its molecular weight is 56kDa. This PLB was extracellularly cloned in the pET21d vector. The ORF is 1620 bp (Genbank accession no. -OM939681) long and codes for a protein 539 amino acid long, with a 15 am...
Source: Experimental Parasitology - August 24, 2023 Category: Parasitology Authors: Rituparna Sarkar Sanjib Kumar Sardar Ajanta Ghosal Koushik Das Yumiko Saito-Nakano Shanta Dutta Tomoyoshi Nozaki Sandipan Ganguly Source Type: research
Targeted drug delivery using beeswax-derived albendazole-loaded solid lipid nanoparticles in Haemonchus contortus, an albendazole-tolerant nematode
Exp Parasitol. 2023 Aug 16:108593. doi: 10.1016/j.exppara.2023.108593. Online ahead of print.ABSTRACTTargeted delivery has not been achieved for anthelmintic treatment, resulting in the requirement of excess drug dose leading to side effects and therapeutic resistance. Gastrointestinal helminths take up lipid droplets from digestive fluid for energy production, egg development, and defense which inspired us to develop biocompatible and orally administrable albendazole-loaded solid lipid nanoparticles (SLN-A) that were derived from beeswax and showed drug loading efficiency of 83.3 ± 6.5 mg/g and sustained-release properti...
Source: Experimental Parasitology - August 18, 2023 Category: Parasitology Authors: Sunidhi Sharma Vanshita Goel Pawandeep Kaur Kundlik Gadhave Neha Garg Lachhman Das Singla Diptiman Choudhury Source Type: research
Targeted drug delivery using beeswax-derived albendazole-loaded solid lipid nanoparticles in Haemonchus contortus, an albendazole-tolerant nematode
Exp Parasitol. 2023 Aug 16:108593. doi: 10.1016/j.exppara.2023.108593. Online ahead of print.ABSTRACTTargeted delivery has not been achieved for anthelmintic treatment, resulting in the requirement of excess drug dose leading to side effects and therapeutic resistance. Gastrointestinal helminths take up lipid droplets from digestive fluid for energy production, egg development, and defense which inspired us to develop biocompatible and orally administrable albendazole-loaded solid lipid nanoparticles (SLN-A) that were derived from beeswax and showed drug loading efficiency of 83.3 ± 6.5 mg/g and sustained-release properti...
Source: Experimental Parasitology - August 18, 2023 Category: Parasitology Authors: Sunidhi Sharma Vanshita Goel Pawandeep Kaur Kundlik Gadhave Neha Garg Lachhman Das Singla Diptiman Choudhury Source Type: research
