Growth inhibitory effect of selected quinones from Indian medicinal plants against Theileria annulata
Exp Parasitol. 2023 Sep 25:108622. doi: 10.1016/j.exppara.2023.108622. Online ahead of print.ABSTRACTTropical Bovine Theileriosis, caused by the protozoan parasite Theileria annulata, poses a significant threat to cattle populations. Currently, Buparvaquone is the sole effective naphthoquinone drug commercially available for its treatment. In our research, we delved into the potential of naturally occurring quinones as alternative treatments. We isolated two quinones, emodin and chrysophanol, from Rheum emodi Wall, and two more, embelin and lawsone, from Embelia ribes Burm.f. and Lawsonia inermis L. respectively. We assess...
Source: Experimental Parasitology - September 27, 2023 Category: Parasitology Authors: Rose Mary Kiriyanthan A Radha Perumal Pandikumar Palavesam Azhahianambi N Madan Savarimuthu Ignacimuthu Source Type: research
Insecticide susceptibility status on Aedes aegypti (Linn) and Aedes albopictus (Skuse) of Chittorgarh district, Rajasthan, India
This study was carried out to check the degree of resistance among these vectors in the Chittorgarh district of Rajasthan, India through standard World Health Organization protocol. The resistance was monitored to pyrethroids i.e., deltamethrin (0.05%), permethrin (0.75%), alphacypermethrin (0.05%); organochlorine i.e., DDT (4%), and an organophosphate larvicide i.e., temephos (0.02 mg/L) in both vector species. Complete resistance to DDT was observed among all tested populations of both species. All tested populations of Aedes albopictus were found susceptible to pyrethroids. Aedes aegypti was found resistant in the Manga...
Source: Experimental Parasitology - September 22, 2023 Category: Parasitology Authors: Pradeep Kumar Jangir Arti Prasad Source Type: research
Insecticide susceptibility status on Aedes aegypti (Linn) and Aedes albopictus (Skuse) of Chittorgarh district, Rajasthan, India
This study was carried out to check the degree of resistance among these vectors in the Chittorgarh district of Rajasthan, India through standard World Health Organization protocol. The resistance was monitored to pyrethroids i.e., deltamethrin (0.05%), permethrin (0.75%), alphacypermethrin (0.05%); organochlorine i.e., DDT (4%), and an organophosphate larvicide i.e., temephos (0.02 mg/L) in both vector species. Complete resistance to DDT was observed among all tested populations of both species. All tested populations of Aedes albopictus were found susceptible to pyrethroids. Aedes aegypti was found resistant in the Manga...
Source: Experimental Parasitology - September 22, 2023 Category: Parasitology Authors: Pradeep Kumar Jangir Arti Prasad Source Type: research
Insecticide susceptibility status on Aedes aegypti (Linn) and Aedes albopictus (Skuse) of Chittorgarh district, Rajasthan, India
This study was carried out to check the degree of resistance among these vectors in the Chittorgarh district of Rajasthan, India through standard World Health Organization protocol. The resistance was monitored to pyrethroids i.e., deltamethrin (0.05%), permethrin (0.75%), alphacypermethrin (0.05%); organochlorine i.e., DDT (4%), and an organophosphate larvicide i.e., temephos (0.02 mg/L) in both vector species. Complete resistance to DDT was observed among all tested populations of both species. All tested populations of Aedes albopictus were found susceptible to pyrethroids. Aedes aegypti was found resistant in the Manga...
Source: Experimental Parasitology - September 22, 2023 Category: Parasitology Authors: Pradeep Kumar Jangir Arti Prasad Source Type: research
Insecticide susceptibility status on Aedes aegypti (Linn) and Aedes albopictus (Skuse) of Chittorgarh district, Rajasthan, India
This study was carried out to check the degree of resistance among these vectors in the Chittorgarh district of Rajasthan, India through standard World Health Organization protocol. The resistance was monitored to pyrethroids i.e., deltamethrin (0.05%), permethrin (0.75%), alphacypermethrin (0.05%); organochlorine i.e., DDT (4%), and an organophosphate larvicide i.e., temephos (0.02 mg/L) in both vector species. Complete resistance to DDT was observed among all tested populations of both species. All tested populations of Aedes albopictus were found susceptible to pyrethroids. Aedes aegypti was found resistant in the Manga...
Source: Experimental Parasitology - September 22, 2023 Category: Parasitology Authors: Pradeep Kumar Jangir Arti Prasad Source Type: research
Evaluation of recombinant Babesia gibsoni thrombospondin-related adhesive protein (BgTRAP) for the sero-diagnosis of canine babesiosis
Exp Parasitol. 2023 Sep 16:108621. doi: 10.1016/j.exppara.2023.108621. Online ahead of print.ABSTRACTCanine babesiosis, caused by Babesia gibsoni is one of the most significant tick-borne illnesses across the world. Light microscopy as well as polymerase chain reaction may fail in the diagnosis of disease when the level of parasitaemia is very low during subclinical and chronic cases. The serological techniques using a recombinant protein will be useful for the accurate and sensitive surveillance of the disease, especially in chronic cases. The present study describes the evaluation of recombinant N-terminal B. gibsoni Thr...
Source: Experimental Parasitology - September 18, 2023 Category: Parasitology Authors: Chundayil Kalarickal Deepa Anju Varghese Karapparambu Gopalan Ajith Kumar Ashwathappa Nandini Gatchanda Shravan Kumar Prabodh Kumar Hembram Chemmangattuvalappil Narendranath Dinesh Sanis Juliet Jess Vergis Ollukkara Krishnan Sindhu Reghu Ravindran Source Type: research
Evaluation of recombinant Babesia gibsoni thrombospondin-related adhesive protein (BgTRAP) for the sero-diagnosis of canine babesiosis
Exp Parasitol. 2023 Sep 16:108621. doi: 10.1016/j.exppara.2023.108621. Online ahead of print.ABSTRACTCanine babesiosis, caused by Babesia gibsoni is one of the most significant tick-borne illnesses across the world. Light microscopy as well as polymerase chain reaction may fail in the diagnosis of disease when the level of parasitaemia is very low during subclinical and chronic cases. The serological techniques using a recombinant protein will be useful for the accurate and sensitive surveillance of the disease, especially in chronic cases. The present study describes the evaluation of recombinant N-terminal B. gibsoni Thr...
Source: Experimental Parasitology - September 18, 2023 Category: Parasitology Authors: Chundayil Kalarickal Deepa Anju Varghese Karapparambu Gopalan Ajith Kumar Ashwathappa Nandini Gatchanda Shravan Kumar Prabodh Kumar Hembram Chemmangattuvalappil Narendranath Dinesh Sanis Juliet Jess Vergis Ollukkara Krishnan Sindhu Reghu Ravindran Source Type: research
Evaluation of recombinant Babesia gibsoni thrombospondin-related adhesive protein (BgTRAP) for the sero-diagnosis of canine babesiosis
Exp Parasitol. 2023 Sep 16:108621. doi: 10.1016/j.exppara.2023.108621. Online ahead of print.ABSTRACTCanine babesiosis, caused by Babesia gibsoni is one of the most significant tick-borne illnesses across the world. Light microscopy as well as polymerase chain reaction may fail in the diagnosis of disease when the level of parasitaemia is very low during subclinical and chronic cases. The serological techniques using a recombinant protein will be useful for the accurate and sensitive surveillance of the disease, especially in chronic cases. The present study describes the evaluation of recombinant N-terminal B. gibsoni Thr...
Source: Experimental Parasitology - September 18, 2023 Category: Parasitology Authors: Chundayil Kalarickal Deepa Anju Varghese Karapparambu Gopalan Ajith Kumar Ashwathappa Nandini Gatchanda Shravan Kumar Prabodh Kumar Hembram Chemmangattuvalappil Narendranath Dinesh Sanis Juliet Jess Vergis Ollukkara Krishnan Sindhu Reghu Ravindran Source Type: research
Evaluation of recombinant Babesia gibsoni thrombospondin-related adhesive protein (BgTRAP) for the sero-diagnosis of canine babesiosis
Exp Parasitol. 2023 Sep 16:108621. doi: 10.1016/j.exppara.2023.108621. Online ahead of print.ABSTRACTCanine babesiosis, caused by Babesia gibsoni is one of the most significant tick-borne illnesses across the world. Light microscopy as well as polymerase chain reaction may fail in the diagnosis of disease when the level of parasitaemia is very low during subclinical and chronic cases. The serological techniques using a recombinant protein will be useful for the accurate and sensitive surveillance of the disease, especially in chronic cases. The present study describes the evaluation of recombinant N-terminal B. gibsoni Thr...
Source: Experimental Parasitology - September 18, 2023 Category: Parasitology Authors: Chundayil Kalarickal Deepa Anju Varghese Karapparambu Gopalan Ajith Kumar Ashwathappa Nandini Gatchanda Shravan Kumar Prabodh Kumar Hembram Chemmangattuvalappil Narendranath Dinesh Sanis Juliet Jess Vergis Ollukkara Krishnan Sindhu Reghu Ravindran Source Type: research
Conditional expression of NanoLuc luciferase through a multimodular system offers rapid detection of antimalarial drug activity
Exp Parasitol. 2023 Sep 14:108620. doi: 10.1016/j.exppara.2023.108620. Online ahead of print.ABSTRACTConditional gene expression is a powerful tool to investigate putative vaccine and drug targets, especially in a haploid organism such as Plasmodium falciparum. Inducible systems based on regulation of either transcription, translation, protein or mRNA stability, among others, allow switching on an off the expression of any desired gene causing specific gain or loss of function phenotypes. However, those systems can be cumbersome involving the construction of large plasmids and generation of multiple transgenic parasite lin...
Source: Experimental Parasitology - September 16, 2023 Category: Parasitology Authors: Caroline Lima Ignasi B Verdaguer Gerhard Wunderlich Alejandro M Katzin Brendan S Crabb Paul R Gilson Mauro F Azevedo Source Type: research
Conditional expression of NanoLuc luciferase through a multimodular system offers rapid detection of antimalarial drug activity
Exp Parasitol. 2023 Sep 14:108620. doi: 10.1016/j.exppara.2023.108620. Online ahead of print.ABSTRACTConditional gene expression is a powerful tool to investigate putative vaccine and drug targets, especially in a haploid organism such as Plasmodium falciparum. Inducible systems based on regulation of either transcription, translation, protein or mRNA stability, among others, allow switching on an off the expression of any desired gene causing specific gain or loss of function phenotypes. However, those systems can be cumbersome involving the construction of large plasmids and generation of multiple transgenic parasite lin...
Source: Experimental Parasitology - September 16, 2023 Category: Parasitology Authors: Caroline Lima Ignasi B Verdaguer Gerhard Wunderlich Alejandro M Katzin Brendan S Crabb Paul R Gilson Mauro F Azevedo Source Type: research
Inhibition of carbonic anhydrase using aspirin is a novel method to block schistosomiasis infection of the parasitic trematode, Schistosoma mansoni, in the intermediate snail host, Biomphalaria glabrata
In this study, we investigated the role of CA inhibition in B. glabrata snails exposed to S. mansoni miracidia. We analyzed the expression of the B. glabrata CA encoding transcript in juvenile susceptible and resistant snails, with and without exposure to S. mansoni. Our results showed that the expression of the CA mRNA encoding transcript was upregulated during early and prolonged infection in susceptible snails (BBO2), but not in the resistant BS-90 stock. Notably, sodium salicylate, a form of aspirin, inhibited the expression of CA, post-exposure, to the parasite. Increasing research between parasites and cancer has sho...
Source: Experimental Parasitology - September 11, 2023 Category: Parasitology Authors: Simone Parn Gabriela Lewis Matty Knight Source Type: research
Biochemical characterization of a novel sphingomyelinase-like protein from the Rhipicephalus microplus tick
Exp Parasitol. 2023 Sep 9;254:108616. doi: 10.1016/j.exppara.2023.108616. Online ahead of print.ABSTRACTSphingomyelinase D is a toxin present in venomous spiders and bacteria and is associated with infection symptoms in patients affected by spider bites. It was observed that in Ixodes scapularis ticks, sphingomyelinase-like protein secreted in saliva can modulate the host immune response, affecting the transmission of flavivirus to the host via exosomes. In this work, a sphingomyelinase D-like protein (RmSMase) from R. microplus, a tick responsible for economic losses and a vector of pathogens for cattle, was investigated....
Source: Experimental Parasitology - September 11, 2023 Category: Parasitology Authors: Fernando A A Silva Gabriel C A Costa Lu ís F Parizi Itabajara da Silva Vaz Junior Aparecida S Tanaka Source Type: research
Inhibition of carbonic anhydrase using aspirin is a novel method to block schistosomiasis infection of the parasitic trematode, Schistosoma mansoni, in the intermediate snail host, Biomphalaria glabrata
In this study, we investigated the role of CA inhibition in B. glabrata snails exposed to S. mansoni miracidia. We analyzed the expression of the B. glabrata CA encoding transcript in juvenile susceptible and resistant snails, with and without exposure to S. mansoni. Our results showed that the expression of the CA mRNA encoding transcript was upregulated during early and prolonged infection in susceptible snails (BBO2), but not in the resistant BS-90 stock. Notably, sodium salicylate, a form of aspirin, inhibited the expression of CA, post-exposure, to the parasite. Increasing research between parasites and cancer has sho...
Source: Experimental Parasitology - September 11, 2023 Category: Parasitology Authors: Simone Parn Gabriela Lewis Matty Knight Source Type: research
Biochemical characterization of a novel sphingomyelinase-like protein from the Rhipicephalus microplus tick
Exp Parasitol. 2023 Sep 9;254:108616. doi: 10.1016/j.exppara.2023.108616. Online ahead of print.ABSTRACTSphingomyelinase D is a toxin present in venomous spiders and bacteria and is associated with infection symptoms in patients affected by spider bites. It was observed that in Ixodes scapularis ticks, sphingomyelinase-like protein secreted in saliva can modulate the host immune response, affecting the transmission of flavivirus to the host via exosomes. In this work, a sphingomyelinase D-like protein (RmSMase) from R. microplus, a tick responsible for economic losses and a vector of pathogens for cattle, was investigated....
Source: Experimental Parasitology - September 11, 2023 Category: Parasitology Authors: Fernando A A Silva Gabriel C A Costa Lu ís F Parizi Itabajara da Silva Vaz Junior Aparecida S Tanaka Source Type: research
