First report of acaricidal efficacy from plumbagin on larvae of Rhipicephalus microplus and Rhipicephalus sanguineus resistant to conventional acaricides
Exp Parasitol. 2023 Oct 11:108632. doi: 10.1016/j.exppara.2023.108632. Online ahead of print.ABSTRACTThe problem of resistance to acaricides in ticks such as Rhipicephalus microplus and R. sanguineus has motivated the search for control alternatives, such as the use of extracts and secondary metabolites from plants. Plumbagin is a natural product present in plants such as Plumbago zeylanica L., Diospyros kaki and D. anisandra, of which acaricidal activity has been reported. Therefore, the objective of this study was to evaluate in vitro the acaricidal efficacy of plumbagin on larvae of R. microplus and R. sanguineus resist...
Source: Experimental Parasitology - October 13, 2023 Category: Parasitology Authors: J R Guti érrez-Wong J A Rosado-Aguilar R I Rodr íguez-Vivas Source Type: research

First report of acaricidal efficacy from plumbagin on larvae of Rhipicephalus microplus and Rhipicephalus sanguineus resistant to conventional acaricides
Exp Parasitol. 2023 Oct 11:108632. doi: 10.1016/j.exppara.2023.108632. Online ahead of print.ABSTRACTThe problem of resistance to acaricides in ticks such as Rhipicephalus microplus and R. sanguineus has motivated the search for control alternatives, such as the use of extracts and secondary metabolites from plants. Plumbagin is a natural product present in plants such as Plumbago zeylanica L., Diospyros kaki and D. anisandra, of which acaricidal activity has been reported. Therefore, the objective of this study was to evaluate in vitro the acaricidal efficacy of plumbagin on larvae of R. microplus and R. sanguineus resist...
Source: Experimental Parasitology - October 13, 2023 Category: Parasitology Authors: J R Guti érrez-Wong J A Rosado-Aguilar R I Rodr íguez-Vivas Source Type: research

First report of acaricidal efficacy from plumbagin on larvae of Rhipicephalus microplus and Rhipicephalus sanguineus resistant to conventional acaricides
Exp Parasitol. 2023 Oct 11:108632. doi: 10.1016/j.exppara.2023.108632. Online ahead of print.ABSTRACTThe problem of resistance to acaricides in ticks such as Rhipicephalus microplus and R. sanguineus has motivated the search for control alternatives, such as the use of extracts and secondary metabolites from plants. Plumbagin is a natural product present in plants such as Plumbago zeylanica L., Diospyros kaki and D. anisandra, of which acaricidal activity has been reported. Therefore, the objective of this study was to evaluate in vitro the acaricidal efficacy of plumbagin on larvae of R. microplus and R. sanguineus resist...
Source: Experimental Parasitology - October 13, 2023 Category: Parasitology Authors: J R Guti érrez-Wong J A Rosado-Aguilar R I Rodr íguez-Vivas Source Type: research

The gene expression and proteomic profiling of Acanthamoeba isolates
CONCLUSION: The gene expression data suggests that lysophospholipase, phospholipase, S8/S53 peptidase, carboxylesterase, and mannose-binding protein (MBP) could play a role in the contact-dependent and independent mechanisms of Acanthamoeba pathogenesis. In addition, the proteomic profiling of the 3 isolates revealed differential protein expression crucial for parasite growth, survival, and virulence. Our results provide baseline data for selecting possible pathogenic targets that could be utilized for designing knockout experiments in the future.PMID:37820893 | DOI:10.1016/j.exppara.2023.108630 (Source: Experimental Parasitology)
Source: Experimental Parasitology - October 11, 2023 Category: Parasitology Authors: Chayan Sharma Sumeeta Khurana Alka Bhatia Amit Arora Amit Gupta Source Type: research

In vitro and In silico assessment of antischistosomal activities of ethanolic extract of Cornulacamonacantha
This study confirms the in vitro schistomicidal activity of C. monacantha extract against S. mansoni adult worms and suggests potential SmCA inhibition.PMID:37820894 | DOI:10.1016/j.exppara.2023.108631 (Source: Experimental Parasitology)
Source: Experimental Parasitology - October 11, 2023 Category: Parasitology Authors: FatmaA S Anwar Asmaa R Abdel-Malek Shimaa H Salem Mohamed Farouk Marwa F Ali Yousef A Abdelrady Ahmed M Sayed Source Type: research

The gene expression and proteomic profiling of Acanthamoeba isolates
CONCLUSION: The gene expression data suggests that lysophospholipase, phospholipase, S8/S53 peptidase, carboxylesterase, and mannose-binding protein (MBP) could play a role in the contact-dependent and independent mechanisms of Acanthamoeba pathogenesis. In addition, the proteomic profiling of the 3 isolates revealed differential protein expression crucial for parasite growth, survival, and virulence. Our results provide baseline data for selecting possible pathogenic targets that could be utilized for designing knockout experiments in the future.PMID:37820893 | DOI:10.1016/j.exppara.2023.108630 (Source: Experimental Parasitology)
Source: Experimental Parasitology - October 11, 2023 Category: Parasitology Authors: Chayan Sharma Sumeeta Khurana Alka Bhatia Amit Arora Amit Gupta Source Type: research

In vitro and In silico assessment of antischistosomal activities of ethanolic extract of Cornulacamonacantha
This study confirms the in vitro schistomicidal activity of C. monacantha extract against S. mansoni adult worms and suggests potential SmCA inhibition.PMID:37820894 | DOI:10.1016/j.exppara.2023.108631 (Source: Experimental Parasitology)
Source: Experimental Parasitology - October 11, 2023 Category: Parasitology Authors: FatmaA S Anwar Asmaa R Abdel-Malek Shimaa H Salem Mohamed Farouk Marwa F Ali Yousef A Abdelrady Ahmed M Sayed Source Type: research

The gene expression and proteomic profiling of Acanthamoeba isolates
CONCLUSION: The gene expression data suggests that lysophospholipase, phospholipase, S8/S53 peptidase, carboxylesterase, and mannose-binding protein (MBP) could play a role in the contact-dependent and independent mechanisms of Acanthamoeba pathogenesis. In addition, the proteomic profiling of the 3 isolates revealed differential protein expression crucial for parasite growth, survival, and virulence. Our results provide baseline data for selecting possible pathogenic targets that could be utilized for designing knockout experiments in the future.PMID:37820893 | DOI:10.1016/j.exppara.2023.108630 (Source: Experimental Parasitology)
Source: Experimental Parasitology - October 11, 2023 Category: Parasitology Authors: Chayan Sharma Sumeeta Khurana Alka Bhatia Amit Arora Amit Gupta Source Type: research

In vitro and In silico assessment of antischistosomal activities of ethanolic extract of Cornulacamonacantha
This study confirms the in vitro schistomicidal activity of C. monacantha extract against S. mansoni adult worms and suggests potential SmCA inhibition.PMID:37820894 | DOI:10.1016/j.exppara.2023.108631 (Source: Experimental Parasitology)
Source: Experimental Parasitology - October 11, 2023 Category: Parasitology Authors: FatmaA S Anwar Asmaa R Abdel-Malek Shimaa H Salem Mohamed Farouk Marwa F Ali Yousef A Abdelrady Ahmed M Sayed Source Type: research

Expansion microscopy of trichomonads
Exp Parasitol. 2023 Oct 4:108629. doi: 10.1016/j.exppara.2023.108629. Online ahead of print.ABSTRACTLight microscopy has significantly advanced in recent decades, especially concerning the increased resolution obtained in fluorescence images. Here we present the Expansion microscopy (ExM) technique in two parasites, Trichomonas vaginalis, and Tritrichomonas foetus, which significantly improved the localization of distinct proteins closely associated with cytoskeleton by immunofluorescence microscopy. The ExM techniques have been used in various cell types, tissues, and other protist parasites. It requires the embedment of ...
Source: Experimental Parasitology - October 6, 2023 Category: Parasitology Authors: Paula Terra Bandeira Sharmila Fiama das Neves Ortiz Marlene Benchimol Wanderley de Souza Source Type: research

Assessing the bioefficacy of a commercial temephos formulation (Temebate ®) for controlling Aedes albopictus larvae in different land use localities in Malaysia
Exp Parasitol. 2023 Oct 5;254:108627. doi: 10.1016/j.exppara.2023.108627. Online ahead of print.ABSTRACTTemephos is the World Health Organization (WHO) recommended larvicide and is still being utilized worldwide to control larvae of dengue vectors; Aedes aegypti and Aedes albopictus. The efficacy of a commercial temephos product; Temebate® to exterminate the local populations of Ae. albopictus larvae originated from different land use particularly dengue-risk and dengue-free housing localities as well as agrarian localities including oil palm plantations, rubber estates and paddy fields was assessed to verify its bioeffic...
Source: Experimental Parasitology - October 6, 2023 Category: Parasitology Authors: Othman Wan-Norafikah Sharifah Aliah-Diyanah Zaifol Atiqah-Izzah Chee Dhang Chen Mohd Sofian-Azirun Asri Lailatul-Nadhirah Mohammad Johari Ibahim Source Type: research

Expansion microscopy of trichomonads
Exp Parasitol. 2023 Oct 4:108629. doi: 10.1016/j.exppara.2023.108629. Online ahead of print.ABSTRACTLight microscopy has significantly advanced in recent decades, especially concerning the increased resolution obtained in fluorescence images. Here we present the Expansion microscopy (ExM) technique in two parasites, Trichomonas vaginalis, and Tritrichomonas foetus, which significantly improved the localization of distinct proteins closely associated with cytoskeleton by immunofluorescence microscopy. The ExM techniques have been used in various cell types, tissues, and other protist parasites. It requires the embedment of ...
Source: Experimental Parasitology - October 6, 2023 Category: Parasitology Authors: Paula Terra Bandeira Sharmila Fiama das Neves Ortiz Marlene Benchimol Wanderley de Souza Source Type: research

Assessing the bioefficacy of a commercial temephos formulation (Temebate ®) for controlling Aedes albopictus larvae in different land use localities in Malaysia
Exp Parasitol. 2023 Oct 4:108627. doi: 10.1016/j.exppara.2023.108627. Online ahead of print.ABSTRACTTemephos is the World Health Organization (WHO) recommended larvicide and is still being utilized worldwide to control larvae of dengue vectors; Aedes aegypti and Aedes albopictus. The efficacy of a commercial temephos product; Temebate® to exterminate the local populations of Ae. albopictus larvae originated from different land use particularly dengue-risk and dengue-free housing localities as well as agrarian localities including oil palm plantations, rubber estates and paddy fields was assessed to verify its bioefficacy ...
Source: Experimental Parasitology - October 6, 2023 Category: Parasitology Authors: Othman Wan-Norafikah Sharifah Aliah-Diyanah Zaifol Atiqah-Izzah Chee Dhang Chen Mohd Sofian-Azirun Asri Lailatul-Nadhirah Mohammad Johari Ibahim Source Type: research

Expansion microscopy of trichomonads
Exp Parasitol. 2023 Oct 4:108629. doi: 10.1016/j.exppara.2023.108629. Online ahead of print.ABSTRACTLight microscopy has significantly advanced in recent decades, especially concerning the increased resolution obtained in fluorescence images. Here we present the Expansion microscopy (ExM) technique in two parasites, Trichomonas vaginalis, and Tritrichomonas foetus, which significantly improved the localization of distinct proteins closely associated with cytoskeleton by immunofluorescence microscopy. The ExM techniques have been used in various cell types, tissues, and other protist parasites. It requires the embedment of ...
Source: Experimental Parasitology - October 6, 2023 Category: Parasitology Authors: Paula Terra Bandeira Sharmila Fiama das Neves Ortiz Marlene Benchimol Wanderley de Souza Source Type: research

Assessing the bioefficacy of a commercial temephos formulation (Temebate ®) for controlling Aedes albopictus larvae in different land use localities in Malaysia
Exp Parasitol. 2023 Oct 4:108627. doi: 10.1016/j.exppara.2023.108627. Online ahead of print.ABSTRACTTemephos is the World Health Organization (WHO) recommended larvicide and is still being utilized worldwide to control larvae of dengue vectors; Aedes aegypti and Aedes albopictus. The efficacy of a commercial temephos product; Temebate® to exterminate the local populations of Ae. albopictus larvae originated from different land use particularly dengue-risk and dengue-free housing localities as well as agrarian localities including oil palm plantations, rubber estates and paddy fields was assessed to verify its bioefficacy ...
Source: Experimental Parasitology - October 6, 2023 Category: Parasitology Authors: Othman Wan-Norafikah Sharifah Aliah-Diyanah Zaifol Atiqah-Izzah Chee Dhang Chen Mohd Sofian-Azirun Asri Lailatul-Nadhirah Mohammad Johari Ibahim Source Type: research