Recent advances in genome editing of bloodstream forms of Trypanosoma congolense using CRISPR-Cas9 ribonucleoproteins: Proof of concept
We present an efficient, reproducible and stable CRISPR-Cas9 genome editing system for use in Tc bloodstream forms (Tc-BSF). This plasmid-free system is based on transient expression of Cas9 protein and the use of a ribonucleoprotein formed by the Cas9 and sgRNA complex. This is the first proof of concept of genome editing using CRISPR-Cas9 ribonucleoproteins on Tc-BSF. This adapted protocol enriches the "toolbox" for the functional study of genes of interest in blood forms of the Trypanosoma congolense. This proof of concept is an important step for the scientific community working on the study of trypanosomes and opens u...
Source: Experimental Parasitology - July 29, 2023 Category: Parasitology Authors: C écile Minet Isabelle Chantal David Berthier Source Type: research
Recent advances in genome editing of bloodstream forms of Trypanosoma congolense using CRISPR-Cas9 ribonucleoproteins: Proof of concept
We present an efficient, reproducible and stable CRISPR-Cas9 genome editing system for use in Tc bloodstream forms (Tc-BSF). This plasmid-free system is based on transient expression of Cas9 protein and the use of a ribonucleoprotein formed by the Cas9 and sgRNA complex. This is the first proof of concept of genome editing using CRISPR-Cas9 ribonucleoproteins on Tc-BSF. This adapted protocol enriches the "toolbox" for the functional study of genes of interest in blood forms of the Trypanosoma congolense. This proof of concept is an important step for the scientific community working on the study of trypanosomes and opens u...
Source: Experimental Parasitology - July 29, 2023 Category: Parasitology Authors: C écile Minet Isabelle Chantal David Berthier Source Type: research
Recent advances in genome editing of bloodstream forms of Trypanosoma congolense using CRISPR-Cas9 ribonucleoproteins: Proof of concept
We present an efficient, reproducible and stable CRISPR-Cas9 genome editing system for use in Tc bloodstream forms (Tc-BSF). This plasmid-free system is based on transient expression of Cas9 protein and the use of a ribonucleoprotein formed by the Cas9 and sgRNA complex. This is the first proof of concept of genome editing using CRISPR-Cas9 ribonucleoproteins on Tc-BSF. This adapted protocol enriches the "toolbox" for the functional study of genes of interest in blood forms of the Trypanosoma congolense. This proof of concept is an important step for the scientific community working on the study of trypanosomes and opens u...
Source: Experimental Parasitology - July 29, 2023 Category: Parasitology Authors: C écile Minet Isabelle Chantal David Berthier Source Type: research
Efficacy of oral administration of ascofuranone with and without glycerol against Trypanosoma congolense
Exp Parasitol. 2023 Jul 25;252:108588. doi: 10.1016/j.exppara.2023.108588. Online ahead of print.ABSTRACTIn many developing countries, trypanosomosis in animals results in the reduction of livestock productivity. Since trypanosomosis is endemic to rural areas where medical and veterinary infrastructure is underdeveloped, development of affordable and easy-to-maintain drugs for treatment and prophylaxis against trypanosomosis is necessary. To this end, in this study, we evaluated the efficacy of oral administration of ascofuranone (AF), with and without glycerol (GOL), against trypanosomosis, using a mouse model. We used T....
Source: Experimental Parasitology - July 27, 2023 Category: Parasitology Authors: Ai Yamazaki Keisuke Suganuma Yusuke Tanaka Kenichi Watanabe Shin-Ichiro Kawazu Kiyoshi Kita Noboru Inoue Source Type: research
Efficacy of oral administration of ascofuranone with and without glycerol against Trypanosoma congolense
Exp Parasitol. 2023 Jul 25:108588. doi: 10.1016/j.exppara.2023.108588. Online ahead of print.ABSTRACTIn many developing countries, trypanosomosis in animals results in the reduction of livestock productivity. Since trypanosomosis is endemic to rural areas where medical and veterinary infrastructure is underdeveloped, development of affordable and easy-to-maintain drugs for treatment and prophylaxis against trypanosomosis is necessary. To this end, in this study, we evaluated the efficacy of oral administration of ascofuranone (AF), with and without glycerol (GOL), against trypanosomosis, using a mouse model. We used T. con...
Source: Experimental Parasitology - July 27, 2023 Category: Parasitology Authors: Ai Yamazaki Keisuke Suganuma Yusuke Tanaka Kenichi Watanabe Shin-Ichiro Kawazu Kiyoshi Kita Noboru Inoue Source Type: research
Larvicidal activity of green synthesized iron oxide nanoparticles using Grevillea robusta Cunn. leaf extract against vector mosquitoes and their characterization
Exp Parasitol. 2023 Jul 17:108586. doi: 10.1016/j.exppara.2023.108586. Online ahead of print.ABSTRACTExtensive fumigation of synthetic pesticides to control the mosquito vector during each post-monsoon season in Pakistan significantly enhanced the environmental contamination and extinction of beneficial insects from the urban ecosystems. In this context, the present study examined the larvicidal efficacy of green synthesized iron nanoparticles (IONPs), using an aqueous leaf extract of Grevillea robusta against the early 2nd and 4th instar larvae of Aedes aegypti and Anopheles stephensi in Pakistan. The prepared IONPs were ...
Source: Experimental Parasitology - July 19, 2023 Category: Parasitology Authors: Faisal Zargham Muhammad Afzal Khadija Rasool Saba Manzoor Naveeda Akhtar Qureshi Source Type: research
Acaricides resistance in Rhipicephalus microplus and expression profile of ABC-transporter genes in the sampled populations
Exp Parasitol. 2023 Jul 17:108584. doi: 10.1016/j.exppara.2023.108584. Online ahead of print.ABSTRACTCurrently, livestock owners manage tick infestations using chemicals, but the method is increasingly losing effectiveness as resistant tick populations have established in the field conditions. Thus, to develop effective tick management strategies, monitoring of resistance in most predominant tick species, Rhipicephalus microplus was targeted. The ticks were collected from eleven districts of Madhya Pradesh and one district of Punjab and tested against deltamethrin (DLM), cypermethrin (CYP), coumaphos (CMP), ivermectin (IVM...
Source: Experimental Parasitology - July 19, 2023 Category: Parasitology Authors: Mukesh Shakya Anil Kumar Sharma Sachin Kumar Deepak Upadhaya Gaurav Nagar Kaushlendra Singh Sankar M Srikant Ghosh Source Type: research
Larvicidal activity of green synthesized iron oxide nanoparticles using Grevillea robusta Cunn. leaf extract against vector mosquitoes and their characterization
Exp Parasitol. 2023 Jul 17:108586. doi: 10.1016/j.exppara.2023.108586. Online ahead of print.ABSTRACTExtensive fumigation of synthetic pesticides to control the mosquito vector during each post-monsoon season in Pakistan significantly enhanced the environmental contamination and extinction of beneficial insects from the urban ecosystems. In this context, the present study examined the larvicidal efficacy of green synthesized iron nanoparticles (IONPs), using an aqueous leaf extract of Grevillea robusta against the early 2nd and 4th instar larvae of Aedes aegypti and Anopheles stephensi in Pakistan. The prepared IONPs were ...
Source: Experimental Parasitology - July 19, 2023 Category: Parasitology Authors: Faisal Zargham Muhammad Afzal Khadija Rasool Saba Manzoor Naveeda Akhtar Qureshi Source Type: research
Acaricides resistance in Rhipicephalus microplus and expression profile of ABC-transporter genes in the sampled populations
Exp Parasitol. 2023 Jul 17:108584. doi: 10.1016/j.exppara.2023.108584. Online ahead of print.ABSTRACTCurrently, livestock owners manage tick infestations using chemicals, but the method is increasingly losing effectiveness as resistant tick populations have established in the field conditions. Thus, to develop effective tick management strategies, monitoring of resistance in most predominant tick species, Rhipicephalus microplus was targeted. The ticks were collected from eleven districts of Madhya Pradesh and one district of Punjab and tested against deltamethrin (DLM), cypermethrin (CYP), coumaphos (CMP), ivermectin (IVM...
Source: Experimental Parasitology - July 19, 2023 Category: Parasitology Authors: Mukesh Shakya Anil Kumar Sharma Sachin Kumar Deepak Upadhaya Gaurav Nagar Kaushlendra Singh Sankar M Srikant Ghosh Source Type: research
Larvicidal activity of green synthesized iron oxide nanoparticles using Grevillea robusta Cunn. leaf extract against vector mosquitoes and their characterization
Exp Parasitol. 2023 Jul 17:108586. doi: 10.1016/j.exppara.2023.108586. Online ahead of print.ABSTRACTExtensive fumigation of synthetic pesticides to control the mosquito vector during each post-monsoon season in Pakistan significantly enhanced the environmental contamination and extinction of beneficial insects from the urban ecosystems. In this context, the present study examined the larvicidal efficacy of green synthesized iron nanoparticles (IONPs), using an aqueous leaf extract of Grevillea robusta against the early 2nd and 4th instar larvae of Aedes aegypti and Anopheles stephensi in Pakistan. The prepared IONPs were ...
Source: Experimental Parasitology - July 19, 2023 Category: Parasitology Authors: Faisal Zargham Muhammad Afzal Khadija Rasool Saba Manzoor Naveeda Akhtar Qureshi Source Type: research
Acaricides resistance in Rhipicephalus microplus and expression profile of ABC-transporter genes in the sampled populations
Exp Parasitol. 2023 Jul 17:108584. doi: 10.1016/j.exppara.2023.108584. Online ahead of print.ABSTRACTCurrently, livestock owners manage tick infestations using chemicals, but the method is increasingly losing effectiveness as resistant tick populations have established in the field conditions. Thus, to develop effective tick management strategies, monitoring of resistance in most predominant tick species, Rhipicephalus microplus was targeted. The ticks were collected from eleven districts of Madhya Pradesh and one district of Punjab and tested against deltamethrin (DLM), cypermethrin (CYP), coumaphos (CMP), ivermectin (IVM...
Source: Experimental Parasitology - July 19, 2023 Category: Parasitology Authors: Mukesh Shakya Anil Kumar Sharma Sachin Kumar Deepak Upadhaya Gaurav Nagar Kaushlendra Singh Sankar M Srikant Ghosh Source Type: research
Larvicidal activity of green synthesized iron oxide nanoparticles using Grevillea robusta Cunn. leaf extract against vector mosquitoes and their characterization
Exp Parasitol. 2023 Jul 17:108586. doi: 10.1016/j.exppara.2023.108586. Online ahead of print.ABSTRACTExtensive fumigation of synthetic pesticides to control the mosquito vector during each post-monsoon season in Pakistan significantly enhanced the environmental contamination and extinction of beneficial insects from the urban ecosystems. In this context, the present study examined the larvicidal efficacy of green synthesized iron nanoparticles (IONPs), using an aqueous leaf extract of Grevillea robusta against the early 2nd and 4th instar larvae of Aedes aegypti and Anopheles stephensi in Pakistan. The prepared IONPs were ...
Source: Experimental Parasitology - July 19, 2023 Category: Parasitology Authors: Faisal Zargham Muhammad Afzal Khadija Rasool Saba Manzoor Naveeda Akhtar Qureshi Source Type: research
Acaricides resistance in Rhipicephalus microplus and expression profile of ABC-transporter genes in the sampled populations
Exp Parasitol. 2023 Jul 17:108584. doi: 10.1016/j.exppara.2023.108584. Online ahead of print.ABSTRACTCurrently, livestock owners manage tick infestations using chemicals, but the method is increasingly losing effectiveness as resistant tick populations have established in the field conditions. Thus, to develop effective tick management strategies, monitoring of resistance in most predominant tick species, Rhipicephalus microplus was targeted. The ticks were collected from eleven districts of Madhya Pradesh and one district of Punjab and tested against deltamethrin (DLM), cypermethrin (CYP), coumaphos (CMP), ivermectin (IVM...
Source: Experimental Parasitology - July 19, 2023 Category: Parasitology Authors: Mukesh Shakya Anil Kumar Sharma Sachin Kumar Deepak Upadhaya Gaurav Nagar Kaushlendra Singh Sankar M Srikant Ghosh Source Type: research
Larvicidal activity of green synthesized iron oxide nanoparticles using Grevillea robusta Cunn. leaf extract against vector mosquitoes and their characterization
Exp Parasitol. 2023 Jul 17:108586. doi: 10.1016/j.exppara.2023.108586. Online ahead of print.ABSTRACTExtensive fumigation of synthetic pesticides to control the mosquito vector during each post-monsoon season in Pakistan significantly enhanced the environmental contamination and extinction of beneficial insects from the urban ecosystems. In this context, the present study examined the larvicidal efficacy of green synthesized iron nanoparticles (IONPs), using an aqueous leaf extract of Grevillea robusta against the early 2nd and 4th instar larvae of Aedes aegypti and Anopheles stephensi in Pakistan. The prepared IONPs were ...
Source: Experimental Parasitology - July 19, 2023 Category: Parasitology Authors: Faisal Zargham Muhammad Afzal Khadija Rasool Saba Manzoor Naveeda Akhtar Qureshi Source Type: research
Acaricides resistance in Rhipicephalus microplus and expression profile of ABC-transporter genes in the sampled populations
Exp Parasitol. 2023 Jul 17:108584. doi: 10.1016/j.exppara.2023.108584. Online ahead of print.ABSTRACTCurrently, livestock owners manage tick infestations using chemicals, but the method is increasingly losing effectiveness as resistant tick populations have established in the field conditions. Thus, to develop effective tick management strategies, monitoring of resistance in most predominant tick species, Rhipicephalus microplus was targeted. The ticks were collected from eleven districts of Madhya Pradesh and one district of Punjab and tested against deltamethrin (DLM), cypermethrin (CYP), coumaphos (CMP), ivermectin (IVM...
Source: Experimental Parasitology - July 19, 2023 Category: Parasitology Authors: Mukesh Shakya Anil Kumar Sharma Sachin Kumar Deepak Upadhaya Gaurav Nagar Kaushlendra Singh Sankar M Srikant Ghosh Source Type: research
