The effects of human and rhesus macaque blood meal sources on mosquito reproduction and adult survival under laboratory conditions
Exp Parasitol. 2023 Aug 7:108591. doi: 10.1016/j.exppara.2023.108591. Online ahead of print.ABSTRACTMass rearing of mosquitoes as required to fulfil research studies is a technically challenging endeavor. Blood meal source has been recognized as a key consideration in mass rearing of mosquitoes that affects colony health and fecundity. Four species of laboratory-colonized mosquitoes from the Department of Entomology, US Army Medical Directorate - Armed Forces Research Institute of Medical Sciences (USAMD-AFRIMS); Anopheles cracens, An. dirus, An. minimus and An. sawadwongporni were fed blood meals from human and rhesus mac...
Source: Experimental Parasitology - August 9, 2023 Category: Parasitology Authors: Siriporn Phasomkusolsil Jaruwan Tawong Nantaporn Monkanna Tanaporn Kornkan Thanin Jitbantrengphan Marisa Chaiyasab Natchanida Pongda Thinadda Kamram Erica J Lindroth Source Type: research
The effects of human and rhesus macaque blood meal sources on mosquito reproduction and adult survival under laboratory conditions
Exp Parasitol. 2023 Aug 7:108591. doi: 10.1016/j.exppara.2023.108591. Online ahead of print.ABSTRACTMass rearing of mosquitoes as required to fulfil research studies is a technically challenging endeavor. Blood meal source has been recognized as a key consideration in mass rearing of mosquitoes that affects colony health and fecundity. Four species of laboratory-colonized mosquitoes from the Department of Entomology, US Army Medical Directorate - Armed Forces Research Institute of Medical Sciences (USAMD-AFRIMS); Anopheles cracens, An. dirus, An. minimus and An. sawadwongporni were fed blood meals from human and rhesus mac...
Source: Experimental Parasitology - August 9, 2023 Category: Parasitology Authors: Siriporn Phasomkusolsil Jaruwan Tawong Nantaporn Monkanna Tanaporn Kornkan Thanin Jitbantrengphan Marisa Chaiyasab Natchanida Pongda Thinadda Kamram Erica J Lindroth Source Type: research
The effects of human and rhesus macaque blood meal sources on mosquito reproduction and adult survival under laboratory conditions
Exp Parasitol. 2023 Aug 7:108591. doi: 10.1016/j.exppara.2023.108591. Online ahead of print.ABSTRACTMass rearing of mosquitoes as required to fulfil research studies is a technically challenging endeavor. Blood meal source has been recognized as a key consideration in mass rearing of mosquitoes that affects colony health and fecundity. Four species of laboratory-colonized mosquitoes from the Department of Entomology, US Army Medical Directorate - Armed Forces Research Institute of Medical Sciences (USAMD-AFRIMS); Anopheles cracens, An. dirus, An. minimus and An. sawadwongporni were fed blood meals from human and rhesus mac...
Source: Experimental Parasitology - August 9, 2023 Category: Parasitology Authors: Siriporn Phasomkusolsil Jaruwan Tawong Nantaporn Monkanna Tanaporn Kornkan Thanin Jitbantrengphan Marisa Chaiyasab Natchanida Pongda Thinadda Kamram Erica J Lindroth Source Type: research
The effects of human and rhesus macaque blood meal sources on mosquito reproduction and adult survival under laboratory conditions
Exp Parasitol. 2023 Aug 7:108591. doi: 10.1016/j.exppara.2023.108591. Online ahead of print.ABSTRACTMass rearing of mosquitoes as required to fulfil research studies is a technically challenging endeavor. Blood meal source has been recognized as a key consideration in mass rearing of mosquitoes that affects colony health and fecundity. Four species of laboratory-colonized mosquitoes from the Department of Entomology, US Army Medical Directorate - Armed Forces Research Institute of Medical Sciences (USAMD-AFRIMS); Anopheles cracens, An. dirus, An. minimus and An. sawadwongporni were fed blood meals from human and rhesus mac...
Source: Experimental Parasitology - August 9, 2023 Category: Parasitology Authors: Siriporn Phasomkusolsil Jaruwan Tawong Nantaporn Monkanna Tanaporn Kornkan Thanin Jitbantrengphan Marisa Chaiyasab Natchanida Pongda Thinadda Kamram Erica J Lindroth Source Type: research
The effects of human and rhesus macaque blood meal sources on mosquito reproduction and adult survival under laboratory conditions
Exp Parasitol. 2023 Aug 7:108591. doi: 10.1016/j.exppara.2023.108591. Online ahead of print.ABSTRACTMass rearing of mosquitoes as required to fulfil research studies is a technically challenging endeavor. Blood meal source has been recognized as a key consideration in mass rearing of mosquitoes that affects colony health and fecundity. Four species of laboratory-colonized mosquitoes from the Department of Entomology, US Army Medical Directorate - Armed Forces Research Institute of Medical Sciences (USAMD-AFRIMS); Anopheles cracens, An. dirus, An. minimus and An. sawadwongporni were fed blood meals from human and rhesus mac...
Source: Experimental Parasitology - August 9, 2023 Category: Parasitology Authors: Siriporn Phasomkusolsil Jaruwan Tawong Nantaporn Monkanna Tanaporn Kornkan Thanin Jitbantrengphan Marisa Chaiyasab Natchanida Pongda Thinadda Kamram Erica J Lindroth Source Type: research
The effects of human and rhesus macaque blood meal sources on mosquito reproduction and adult survival under laboratory conditions
Exp Parasitol. 2023 Aug 7:108591. doi: 10.1016/j.exppara.2023.108591. Online ahead of print.ABSTRACTMass rearing of mosquitoes as required to fulfil research studies is a technically challenging endeavor. Blood meal source has been recognized as a key consideration in mass rearing of mosquitoes that affects colony health and fecundity. Four species of laboratory-colonized mosquitoes from the Department of Entomology, US Army Medical Directorate - Armed Forces Research Institute of Medical Sciences (USAMD-AFRIMS); Anopheles cracens, An. dirus, An. minimus and An. sawadwongporni were fed blood meals from human and rhesus mac...
Source: Experimental Parasitology - August 9, 2023 Category: Parasitology Authors: Siriporn Phasomkusolsil Jaruwan Tawong Nantaporn Monkanna Tanaporn Kornkan Thanin Jitbantrengphan Marisa Chaiyasab Natchanida Pongda Thinadda Kamram Erica J Lindroth Source Type: research
The effects of human and rhesus macaque blood meal sources on mosquito reproduction and adult survival under laboratory conditions
Exp Parasitol. 2023 Aug 7:108591. doi: 10.1016/j.exppara.2023.108591. Online ahead of print.ABSTRACTMass rearing of mosquitoes as required to fulfil research studies is a technically challenging endeavor. Blood meal source has been recognized as a key consideration in mass rearing of mosquitoes that affects colony health and fecundity. Four species of laboratory-colonized mosquitoes from the Department of Entomology, US Army Medical Directorate - Armed Forces Research Institute of Medical Sciences (USAMD-AFRIMS); Anopheles cracens, An. dirus, An. minimus and An. sawadwongporni were fed blood meals from human and rhesus mac...
Source: Experimental Parasitology - August 9, 2023 Category: Parasitology Authors: Siriporn Phasomkusolsil Jaruwan Tawong Nantaporn Monkanna Tanaporn Kornkan Thanin Jitbantrengphan Marisa Chaiyasab Natchanida Pongda Thinadda Kamram Erica J Lindroth Source Type: research
Efficacy of Duddingtonia flagrans (Bioverm ®) on the biological control of buffalo gastrointestinal nematodes
Exp Parasitol. 2023 Aug 5;253:108592. doi: 10.1016/j.exppara.2023.108592. Online ahead of print.ABSTRACTWe evaluated the efficacy of Bioverm®, a commercial product containing Duddingtonia flagrans, on the control of buffalo (Bubalus bubalis) gastrointestinal nematodes. We randomly divided 12 buffaloes into two groups of six animals. In the treated group, each animal received a Bioverm®`s single dose of 1g (105 chlamydospores of D. flagrans) to 10 kg of live weight; in the control group, each animal received 1g of corn bran for each 10 kg of live weight as a placebo. Fecal samples were individually collected from 12, 24, ...
Source: Experimental Parasitology - August 7, 2023 Category: Parasitology Authors: Luanderson Queiroz Mendes Carolina Magri Ferraz Natalia Rein ó Castro Ribeiro Kim Borja Ulfeldt Jo ão Carlos Corrêa Ribeiro Mayara Faneli Merizio Gabriel Augusto Marques Rossi Ant ônia Aniellen Raianne Moisés Aguiar Jackson Victor de Ara újo Filippe Source Type: research
Efficacy of Duddingtonia flagrans (Bioverm ®) on the biological control of buffalo gastrointestinal nematodes
Exp Parasitol. 2023 Aug 5:108592. doi: 10.1016/j.exppara.2023.108592. Online ahead of print.ABSTRACTWe evaluated the efficacy of Bioverm®, a commercial product containing Duddingtonia flagrans, on the control of buffalo (Bubalus bubalis) gastrointestinal nematodes. We randomly divided 12 buffaloes into two groups of six animals. In the treated group, each animal received a Bioverm®`s single dose of 1g (105 chlamydospores of D. flagrans) to 10 kg of live weight; in the control group, each animal received 1g of corn bran for each 10 kg of live weight as a placebo. Fecal samples were individually collected from 12, 24, 36, ...
Source: Experimental Parasitology - August 7, 2023 Category: Parasitology Authors: Luanderson Queiroz Mendes Carolina Magri Ferraz Natalia Rein ó Castro Ribeiro Kim Borja Ulfeldt Jo ão Carlos Corrêa Ribeiro Mayara Faneli Merizio Gabriel Augusto Marques Rossi Ant ônia Aniellen Raianne Moisés Aguiar Jackson Victor de Ara újo Filippe Source Type: research
Molecular variability of the Ancylostoma secreted Protein-2 (Aca-asp-2) gene from Ancylostoma caninum contributes to expand information on population genetic studies of hookworms
Exp Parasitol. 2023 Aug 4:108590. doi: 10.1016/j.exppara.2023.108590. Online ahead of print.ABSTRACTHookworm infection is a major public health problem in many regions of the world. Given the high levels of host morbidity and even mortality of the host caused by these infections, it is crucial to understand the genetic structure of hookworm populations. This understanding can provide insights into the ecology, transmission patterns, mechanisms of drug resistance, and the development of vaccines and immunotherapeutic strategies. Previously, we examined presumably neutral molecular markers, such as microsatellites and COI (C...
Source: Experimental Parasitology - August 6, 2023 Category: Parasitology Authors: Luis Fernando Viana Furtado Rodrigo Rodrigues Cambraia de Miranda Jacob Adam Tennessen Michael Scott Blouin Élida Mara Leite Rabelo Source Type: research
Molecular variability of the Ancylostoma secreted Protein-2 (Aca-asp-2) gene from Ancylostoma caninum contributes to expand information on population genetic studies of hookworms
Exp Parasitol. 2023 Aug 4:108590. doi: 10.1016/j.exppara.2023.108590. Online ahead of print.ABSTRACTHookworm infection is a major public health problem in many regions of the world. Given the high levels of host morbidity and even mortality of the host caused by these infections, it is crucial to understand the genetic structure of hookworm populations. This understanding can provide insights into the ecology, transmission patterns, mechanisms of drug resistance, and the development of vaccines and immunotherapeutic strategies. Previously, we examined presumably neutral molecular markers, such as microsatellites and COI (C...
Source: Experimental Parasitology - August 6, 2023 Category: Parasitology Authors: Luis Fernando Viana Furtado Rodrigo Rodrigues Cambraia de Miranda Jacob Adam Tennessen Michael Scott Blouin Élida Mara Leite Rabelo Source Type: research
Recent advances in genome editing of bloodstream forms of Trypanosoma congolense using CRISPR-Cas9 ribonucleoproteins: Proof of concept
We present an efficient, reproducible and stable CRISPR-Cas9 genome editing system for use in Tc bloodstream forms (Tc-BSF). This plasmid-free system is based on transient expression of Cas9 protein and the use of a ribonucleoprotein formed by the Cas9 and sgRNA complex. This is the first proof of concept of genome editing using CRISPR-Cas9 ribonucleoproteins on Tc-BSF. This adapted protocol enriches the "toolbox" for the functional study of genes of interest in blood forms of the Trypanosoma congolense. This proof of concept is an important step for the scientific community working on the study of trypanosomes and opens u...
Source: Experimental Parasitology - July 29, 2023 Category: Parasitology Authors: C écile Minet Isabelle Chantal David Berthier Source Type: research
Recent advances in genome editing of bloodstream forms of Trypanosoma congolense using CRISPR-Cas9 ribonucleoproteins: Proof of concept
We present an efficient, reproducible and stable CRISPR-Cas9 genome editing system for use in Tc bloodstream forms (Tc-BSF). This plasmid-free system is based on transient expression of Cas9 protein and the use of a ribonucleoprotein formed by the Cas9 and sgRNA complex. This is the first proof of concept of genome editing using CRISPR-Cas9 ribonucleoproteins on Tc-BSF. This adapted protocol enriches the "toolbox" for the functional study of genes of interest in blood forms of the Trypanosoma congolense. This proof of concept is an important step for the scientific community working on the study of trypanosomes and opens u...
Source: Experimental Parasitology - July 29, 2023 Category: Parasitology Authors: C écile Minet Isabelle Chantal David Berthier Source Type: research
Recent advances in genome editing of bloodstream forms of Trypanosoma congolense using CRISPR-Cas9 ribonucleoproteins: Proof of concept
We present an efficient, reproducible and stable CRISPR-Cas9 genome editing system for use in Tc bloodstream forms (Tc-BSF). This plasmid-free system is based on transient expression of Cas9 protein and the use of a ribonucleoprotein formed by the Cas9 and sgRNA complex. This is the first proof of concept of genome editing using CRISPR-Cas9 ribonucleoproteins on Tc-BSF. This adapted protocol enriches the "toolbox" for the functional study of genes of interest in blood forms of the Trypanosoma congolense. This proof of concept is an important step for the scientific community working on the study of trypanosomes and opens u...
Source: Experimental Parasitology - July 29, 2023 Category: Parasitology Authors: C écile Minet Isabelle Chantal David Berthier Source Type: research
Recent advances in genome editing of bloodstream forms of Trypanosoma congolense using CRISPR-Cas9 ribonucleoproteins: Proof of concept
We present an efficient, reproducible and stable CRISPR-Cas9 genome editing system for use in Tc bloodstream forms (Tc-BSF). This plasmid-free system is based on transient expression of Cas9 protein and the use of a ribonucleoprotein formed by the Cas9 and sgRNA complex. This is the first proof of concept of genome editing using CRISPR-Cas9 ribonucleoproteins on Tc-BSF. This adapted protocol enriches the "toolbox" for the functional study of genes of interest in blood forms of the Trypanosoma congolense. This proof of concept is an important step for the scientific community working on the study of trypanosomes and opens u...
Source: Experimental Parasitology - July 29, 2023 Category: Parasitology Authors: C écile Minet Isabelle Chantal David Berthier Source Type: research
