Stimulation of microvesicle secretion in Trichomonas vaginalis
Exp Parasitol. 2024 Feb 21;259:108722. doi: 10.1016/j.exppara.2024.108722. Online ahead of print.ABSTRACTTrichomonas vaginalis is an extracellular flagellate protozoan and the etiological agent of human trichomoniasis, a sexually transmitted infection (STI) with a high incidence. Several reports have shown that this protozoan releases microvesicles into the culture medium, which show high potential in modulating cell-to-cell communication and the host response to infections. However, the biogenesis of these vesicles has not been analyzed in detail. In the present study, high-resolution ion scanning microscopy (SEM) and tra...
Source: Experimental Parasitology - February 23, 2024 Category: Parasitology Authors: J úlio César Santana de Andrade Marlene Benchimol Wanderley de Souza Source Type: research
Stimulation of microvesicle secretion in Trichomonas vaginalis
Exp Parasitol. 2024 Feb 21;259:108722. doi: 10.1016/j.exppara.2024.108722. Online ahead of print.ABSTRACTTrichomonas vaginalis is an extracellular flagellate protozoan and the etiological agent of human trichomoniasis, a sexually transmitted infection (STI) with a high incidence. Several reports have shown that this protozoan releases microvesicles into the culture medium, which show high potential in modulating cell-to-cell communication and the host response to infections. However, the biogenesis of these vesicles has not been analyzed in detail. In the present study, high-resolution ion scanning microscopy (SEM) and tra...
Source: Experimental Parasitology - February 23, 2024 Category: Parasitology Authors: J úlio César Santana de Andrade Marlene Benchimol Wanderley de Souza Source Type: research
Stimulation of microvesicle secretion in Trichomonas vaginalis
Exp Parasitol. 2024 Feb 21:108722. doi: 10.1016/j.exppara.2024.108722. Online ahead of print.ABSTRACTTrichomonas vaginalis is an extracellular flagellate protozoan and the etiological agent of human trichomoniasis, a sexually transmitted infection (STI) with a high incidence. Several reports have shown that this protozoan releases microvesicles into the culture medium, which show high potential in modulating cell-to-cell communication and the host response to infections. However, the biogenesis of these vesicles has not been analyzed in detail. In the present study, high-resolution ion scanning microscopy (SEM) and transmi...
Source: Experimental Parasitology - February 23, 2024 Category: Parasitology Authors: J úlio César Santana de Andrade Marlene Benchimol Wanderley de Souza Source Type: research
Manganese- and zinc-coordinated interaction of Schistosoma japonicum glutathione S-transferase with neurotransmitter transporters GlyT1 and GAT3 in vitro
Exp Parasitol. 2024 Feb 17;259:108721. doi: 10.1016/j.exppara.2024.108721. Online ahead of print.ABSTRACTGlutathione S-transferases (GSTs) are a family of multifunctional isoenzymes involved in the neutralization of toxic compounds, drug resistance and several other cellular functions. The glutathione S-transferase enzyme of Schistosoma japonicum (SjGST-26) plays a role in human schistosomiasis and is also a frequently used fusion partner in mammalian and bacterial expression and pull-down systems. GSTs seem not to be naturally associated with metal ions. Exceptionally, in vitro, metal binding sites have been previously de...
Source: Experimental Parasitology - February 18, 2024 Category: Parasitology Authors: Martina Baliova Frantisek Jursky Source Type: research
A potential role of protein extractions from Metagonimus yokogawai in amelionating inflammation in patients with ankylosing spondylitis
This study aimed to investigate the potential of Metagonimus yokogawai-derived proteins (MYp) to provide protection against ankylosing spondylitis (AS) through modulation of immune responses. The cytotoxicity of MYp at various doses was first assessed using MTS and flow cytometry. Peripheral blood mononuclear cells (PBMCs) were collected from AS patients, and the production of inflammatory cytokines was analyzed through flow cytometry. In the experiments with SKG mice, MYp or vehicle was administered and inflammation was evaluated through immunohistochemistry and enzyme-linked immunosorbent assay. The results showed that M...
Source: Experimental Parasitology - February 18, 2024 Category: Parasitology Authors: Eun Jeong Won Yu Jeong Lee Moon-Ju Kim Hae-In Lee Hyun Hee Jang Seong Hoon Kim Hee Min Yoo Namki Cho Seung Cheol Shim Tae-Jong Kim Source Type: research
Manganese- and zinc-coordinated interaction of Schistosoma japonicum glutathione S-transferase with neurotransmitter transporters GlyT1 and GAT3 in vitro
Exp Parasitol. 2024 Feb 17;259:108721. doi: 10.1016/j.exppara.2024.108721. Online ahead of print.ABSTRACTGlutathione S-transferases (GSTs) are a family of multifunctional isoenzymes involved in the neutralization of toxic compounds, drug resistance and several other cellular functions. The glutathione S-transferase enzyme of Schistosoma japonicum (SjGST-26) plays a role in human schistosomiasis and is also a frequently used fusion partner in mammalian and bacterial expression and pull-down systems. GSTs seem not to be naturally associated with metal ions. Exceptionally, in vitro, metal binding sites have been previously de...
Source: Experimental Parasitology - February 18, 2024 Category: Parasitology Authors: Martina Baliova Frantisek Jursky Source Type: research
A potential role of protein extractions from Metagonimus yokogawai in amelionating inflammation in patients with ankylosing spondylitis
This study aimed to investigate the potential of Metagonimus yokogawai-derived proteins (MYp) to provide protection against ankylosing spondylitis (AS) through modulation of immune responses. The cytotoxicity of MYp at various doses was first assessed using MTS and flow cytometry. Peripheral blood mononuclear cells (PBMCs) were collected from AS patients, and the production of inflammatory cytokines was analyzed through flow cytometry. In the experiments with SKG mice, MYp or vehicle was administered and inflammation was evaluated through immunohistochemistry and enzyme-linked immunosorbent assay. The results showed that M...
Source: Experimental Parasitology - February 18, 2024 Category: Parasitology Authors: Eun Jeong Won Yu Jeong Lee Moon-Ju Kim Hae-In Lee Hyun Hee Jang Seong Hoon Kim Hee Min Yoo Namki Cho Seung Cheol Shim Tae-Jong Kim Source Type: research
Manganese- and zinc-coordinated interaction of Schistosoma japonicum glutathione S-transferase with neurotransmitter transporters GlyT1 and GAT3 in vitro
Exp Parasitol. 2024 Feb 16:108721. doi: 10.1016/j.exppara.2024.108721. Online ahead of print.ABSTRACTGlutathione S-transferases (GSTs) are a family of multifunctional isoenzymes involved in the neutralization of toxic compounds, drug resistance and several other cellular functions. The glutathione S-transferase enzyme of Schistosoma japonicum (SjGST-26) plays a role in human schistosomiasis and is also a frequently used fusion partner in mammalian and bacterial expression and pull-down systems. GSTs seem not to be naturally associated with metal ions. Exceptionally, in vitro, metal binding sites have been previously descri...
Source: Experimental Parasitology - February 18, 2024 Category: Parasitology Authors: Martina Baliova Frantisek Jursky Source Type: research
A potential role of protein extractions from Metagonimus yokogawai in amelionating inflammation in patients with ankylosing spondylitis
This study aimed to investigate the potential of Metagonimus yokogawai-derived proteins (MYp) to provide protection against ankylosing spondylitis (AS) through modulation of immune responses. The cytotoxicity of MYp at various doses was first assessed using MTS and flow cytometry. Peripheral blood mononuclear cells (PBMCs) were collected from AS patients, and the production of inflammatory cytokines was analyzed through flow cytometry. In the experiments with SKG mice, MYp or vehicle was administered and inflammation was evaluated through immunohistochemistry and enzyme-linked immunosorbent assay. The results showed that M...
Source: Experimental Parasitology - February 18, 2024 Category: Parasitology Authors: Eun Jeong Won Yu Jeong Lee Moon-Ju Kim Hae-In Lee Hyun Hee Jang Seong Hoon Kim Hee Min Yoo Namki Cho Seung Cheol Shim Tae-Jong Kim Source Type: research
Manganese- and zinc-coordinated interaction of Schistosoma japonicum glutathione S-transferase with neurotransmitter transporters GlyT1 and GAT3 in vitro
Exp Parasitol. 2024 Feb 16:108721. doi: 10.1016/j.exppara.2024.108721. Online ahead of print.ABSTRACTGlutathione S-transferases (GSTs) are a family of multifunctional isoenzymes involved in the neutralization of toxic compounds, drug resistance and several other cellular functions. The glutathione S-transferase enzyme of Schistosoma japonicum (SjGST-26) plays a role in human schistosomiasis and is also a frequently used fusion partner in mammalian and bacterial expression and pull-down systems. GSTs seem not to be naturally associated with metal ions. Exceptionally, in vitro, metal binding sites have been previously descri...
Source: Experimental Parasitology - February 18, 2024 Category: Parasitology Authors: Martina Baliova Frantisek Jursky Source Type: research
A potential role of protein extractions from Metagonimus yokogawai in amelionating inflammation in patients with ankylosing spondylitis
This study aimed to investigate the potential of Metagonimus yokogawai-derived proteins (MYp) to provide protection against ankylosing spondylitis (AS) through modulation of immune responses. The cytotoxicity of MYp at various doses was first assessed using MTS and flow cytometry. Peripheral blood mononuclear cells (PBMCs) were collected from AS patients, and the production of inflammatory cytokines was analyzed through flow cytometry. In the experiments with SKG mice, MYp or vehicle was administered and inflammation was evaluated through immunohistochemistry and enzyme-linked immunosorbent assay. The results showed that M...
Source: Experimental Parasitology - February 18, 2024 Category: Parasitology Authors: Eun Jeong Won Yu Jeong Lee Moon-Ju Kim Hae-In Lee Hyun Hee Jang Seong Hoon Kim Hee Min Yoo Namki Cho Seung Cheol Shim Tae-Jong Kim Source Type: research
Manganese- and zinc-coordinated interaction of Schistosoma japonicum glutathione S-transferase with neurotransmitter transporters GlyT1 and GAT3 in vitro
Exp Parasitol. 2024 Feb 16:108721. doi: 10.1016/j.exppara.2024.108721. Online ahead of print.ABSTRACTGlutathione S-transferases (GSTs) are a family of multifunctional isoenzymes involved in the neutralization of toxic compounds, drug resistance and several other cellular functions. The glutathione S-transferase enzyme of Schistosoma japonicum (SjGST-26) plays a role in human schistosomiasis and is also a frequently used fusion partner in mammalian and bacterial expression and pull-down systems. GSTs seem not to be naturally associated with metal ions. Exceptionally, in vitro, metal binding sites have been previously descri...
Source: Experimental Parasitology - February 18, 2024 Category: Parasitology Authors: Martina Baliova Frantisek Jursky Source Type: research
A potential role of protein extractions from Metagonimus yokogawai in amelionating inflammation in patients with ankylosing spondylitis
This study aimed to investigate the potential of Metagonimus yokogawai-derived proteins (MYp) to provide protection against ankylosing spondylitis (AS) through modulation of immune responses. The cytotoxicity of MYp at various doses was first assessed using MTS and flow cytometry. Peripheral blood mononuclear cells (PBMCs) were collected from AS patients, and the production of inflammatory cytokines was analyzed through flow cytometry. In the experiments with SKG mice, MYp or vehicle was administered and inflammation was evaluated through immunohistochemistry and enzyme-linked immunosorbent assay. The results showed that M...
Source: Experimental Parasitology - February 18, 2024 Category: Parasitology Authors: Eun Jeong Won Yu Jeong Lee Moon-Ju Kim Hae-In Lee Hyun Hee Jang Seong Hoon Kim Hee Min Yoo Namki Cho Seung Cheol Shim Tae-Jong Kim Source Type: research
The larvicidal effect of the supernatant of Lactobacillus acidophilus ATCC 4356 on Toxocara canis
Exp Parasitol. 2024 Feb 15:108720. doi: 10.1016/j.exppara.2024.108720. Online ahead of print.ABSTRACTHuman toxocariasis is a parasitic anthropozoonosis that is difficult to treat and control. A previous study carried out with Lactobacillus acidophilus ATCC 4356 revealed that the cell free supernatant (CFS) of this probiotic killed 100% of Toxocara canis larvae in vitro. The present study aimed to investigate the characteristics of the CFS of L. acidophilus ATCC 4356, which may be involved in its larvicidal effects on T. canis. L. acidophilus ATCC 4356 was cultured, and lactic and acetic acids present in the CFS were quanti...
Source: Experimental Parasitology - February 17, 2024 Category: Parasitology Authors: Carolina Netto de Oliveira da Cunha St éfani Rodeghiero Collares D ébora Carvalho Rodrigues D ébora Liliane Walcher Micaele Quintana de Moura Lourdes Helena Rodrigues Martins Gabriel Baracy Klafke Jean Lucas de Oliveira Arias Rodolfo Carapelli Jo ão B Source Type: research
Assessing the effectiveness of fenbendazole supramolecular complexes against schistosomiasis, monieziosis and parabronemosis in sheep
This study explores the potential of supramolecular complexes, created through mechanochemical modifications, to address these limitations. The study focuses on two key anthelmintics, praziquantel (PZQ) and fenbendazole (FBZ), employing mechanochemical techniques to enhance their solubility and efficacy. Solid dispersions (SD) of PZQ with polymers and dioctyl sulfosuccine sodium (DSS) and fenbendazole with essential oils licorice extract (ES) and DSS were prepared. The helminthicidal activity of these complexes was assessed through helminthological dissections of sheep infected with Schistosoma turkestanicum, moniesiasis, ...
Source: Experimental Parasitology - February 17, 2024 Category: Parasitology Authors: Firuza Akramova Ulugbek Shakarbaev Dilbar Paluaniyazova Abror Akbarov Salavat Khalikov Alisher Safarov Djalaliddin Azimov Mourad Ben Said Source Type: research
