Evaluation of the immunoprotective effect of the recombinant Eimeria intestinalis rhoptry protein 25 and rhoptry protein 30 on New Zealand rabbits
CONCLUSION: Immunization of rabbits indicated that both rEiROP25 and rEiROP30 are capable of inducing an increase in specific antibody levels. rEiROP25 triggered a Th1-type immune protection response, while rEiROP30 elicited a Th1/Th2 mixed response. EiROP25 and EiROP30 can generate a moderate level of immune protection, with better efficacy observed for EiROP30. This study provides valuable insights for the promotion of recombinant subunit vaccines targeting rabbit E. intestinalis infection.PMID:38364954 | DOI:10.1016/j.exppara.2024.108719 (Source: Experimental Parasitology)
Source: Experimental Parasitology - February 16, 2024 Category: Parasitology Authors: Ge Hao Changming Xiong Jie Xiao Wei He Yuhua Zhu Liwen Xu Qing Jiang Guangyou Yang Source Type: research
Evaluation of the immunoprotective effect of the recombinant Eimeria intestinalis rhoptry protein 25 and rhoptry protein 30 on New Zealand rabbits
CONCLUSION: Immunization of rabbits indicated that both rEiROP25 and rEiROP30 are capable of inducing an increase in specific antibody levels. rEiROP25 triggered a Th1-type immune protection response, while rEiROP30 elicited a Th1/Th2 mixed response. EiROP25 and EiROP30 can generate a moderate level of immune protection, with better efficacy observed for EiROP30. This study provides valuable insights for the promotion of recombinant subunit vaccines targeting rabbit E. intestinalis infection.PMID:38364954 | DOI:10.1016/j.exppara.2024.108719 (Source: Experimental Parasitology)
Source: Experimental Parasitology - February 16, 2024 Category: Parasitology Authors: Ge Hao Changming Xiong Jie Xiao Wei He Yuhua Zhu Liwen Xu Qing Jiang Guangyou Yang Source Type: research
In vitro antitrypanosomal activity of synthesized nitrofurantoin-triazole hybrids against Trypanosoma species causing animal African trypanosomosis
This study assessed previously synthesized nineteen nitrofurantoin-triazole (NFT-TZ) hybrids against animal trypanosomes and evaluated their cytotoxicity using Madin-Darby bovine kidney cells. The n-alkyl sub-series hybrids, 8 (IC50 0.09 ± 0.02 μM; SI 686.45) and 9 (IC50 0.07 ± 0.04 μM; SI 849.31) had the highest anti-trypanosomal activity against T. b. brucei. On the contrary, the nonyl 6 (IC50 0.12 ± 0.06 μM; SI 504.57) and nitrobenzyl 18 (IC50 0.11 ± 0.03 μM; SI 211.07) displayed the highest trypanocidal activity against T. evansi. The nonyl hybrid 6 (IC50 0.02 ± 0.01 μM; SI 6328.76) was also detected alongsid...
Source: Experimental Parasitology - February 14, 2024 Category: Parasitology Authors: Anna Seetsi David D N'Da Nthatisi Nyembe Keisuke Suganuma Tsepo Ramatla Oriel Thekisoe Source Type: research
In vitro antitrypanosomal activity of synthesized nitrofurantoin-triazole hybrids against Trypanosoma species causing animal African trypanosomosis
This study assessed previously synthesized nineteen nitrofurantoin-triazole (NFT-TZ) hybrids against animal trypanosomes and evaluated their cytotoxicity using Madin-Darby bovine kidney cells. The n-alkyl sub-series hybrids, 8 (IC50 0.09 ± 0.02 μM; SI 686.45) and 9 (IC50 0.07 ± 0.04 μM; SI 849.31) had the highest anti-trypanosomal activity against T. b. brucei. On the contrary, the nonyl 6 (IC50 0.12 ± 0.06 μM; SI 504.57) and nitrobenzyl 18 (IC50 0.11 ± 0.03 μM; SI 211.07) displayed the highest trypanocidal activity against T. evansi. The nonyl hybrid 6 (IC50 0.02 ± 0.01 μM; SI 6328.76) was also detected alongsid...
Source: Experimental Parasitology - February 14, 2024 Category: Parasitology Authors: Anna Seetsi David D N'Da Nthatisi Nyembe Keisuke Suganuma Tsepo Ramatla Oriel Thekisoe Source Type: research
In vitro growth of Leishmania parasites from biopsy samples of suspected cutaneous and visceral leishmaniasis cases in Sri Lanka: An observational study
Exp Parasitol. 2024 Feb 11:108710. doi: 10.1016/j.exppara.2024.108710. Online ahead of print.ABSTRACTSri Lanka reports a large focus of Leishmania donovani caused cutaneous leishmaniasis (CL). Subsequent emergence of visceral leishmaniasis (VL) was also reported recently. Expansion of the on-going disease outbreak and many complexities indicate urgent need to enhance early case detection methods. In vitro cultivation (IVC) of parasites causing visceral leishmaniasis (VL) is important for disease confirmation and to obtain sufficient quantities of parasites required in many scientific studies. IVC is carried out as a useful...
Source: Experimental Parasitology - February 13, 2024 Category: Parasitology Authors: Bhagya Deepachandi Sudath Weerasinghe Himali Gunathileka Preethi Soysa Yamuna Siriwardana Source Type: research
A review of non-invasive samples and tools in kala-azar diagnosis and test of cure
Exp Parasitol. 2024 Feb 11:108713. doi: 10.1016/j.exppara.2024.108713. Online ahead of print.ABSTRACTThe recurrence of visceral leishmaniasis (VL), also called kala-azar (KA), in endemic regions of tropical countries like India, is primarily attributed to asymptomatic VL, post-kala azar dermal leishmaniasis (PKDL), and human immunodeficiency virus (HIV) co-infection. To effectively manage VL cases and elimination targets, an early and rapid diagnosis as well as accurate field surveillance is highly essential. The traditional sampling methods like bone marrow (BM), spleen, and lymph node (LN) tissue aspirations are invasive...
Source: Experimental Parasitology - February 13, 2024 Category: Parasitology Authors: Saravanan Vijayakumar Priyanka Kriti Narayan Shobha Kumari Ravi Ranjan Vikash Kumar Ashish Kumar Dayakar Alti Source Type: research
Activity of alkoxyamide-based histone deacetylase inhibitors against Plasmodium falciparum malaria parasites
Exp Parasitol. 2024 Feb 8:108716. doi: 10.1016/j.exppara.2024.108716. Online ahead of print.ABSTRACTThere are more than 240 million cases of malaria and 600,000 associated deaths each year, most due to infection with Plasmodium falciparum parasites. While malaria treatment options exist, new drugs with novel modes of action are needed to address malaria parasite drug resistance. Protein lysine deacetylases (termed HDACs) are important epigenetic regulatory enzymes and prospective therapeutic targets for malaria. Here we report the antiplasmodial activity of a panel of 17 hydroxamate zinc binding group HDAC inhibitors with ...
Source: Experimental Parasitology - February 10, 2024 Category: Parasitology Authors: Wisam A Dawood Gillian Fisher Franziska Kinnen Christian Anzenhofer Tina Skinner-Adams Leandro Alves Avelar Yodita Asfaha Thomas Kurz Katherine T Andrews Source Type: research
Potential of Ocotea indecora (Schott) Mez essential oil nanoemulsion in schistosomiasis control: Molluscicidal effects
Exp Parasitol. 2024 Feb 8:108717. doi: 10.1016/j.exppara.2024.108717. Online ahead of print.ABSTRACTSchistosomiasis is a neglected disease transmitted through contaminated water in populations with low basic sanitation. The World Health Organization recommends controlling the intermediate host snails of the Biomphalaria genus with the molluscicide niclosamide. This work aims to evaluate the biocidal potential of the nanoemulsion prepared with the essential oil of Ocotea indecora leaves for the control of the mollusk Biomphalaria glabrata, intermediate host of the Schistosoma mansoni, the etiologic agent of schistosomiasis....
Source: Experimental Parasitology - February 10, 2024 Category: Parasitology Authors: Francisco Paiva Machado Leonardo da Silva Rangel Keyla Farias Gomes Jos é Augusto Albuquerque Dos Santos Robson Xavier Faria Marcelo G Santos Caio P Fernandes Leandro Rocha Source Type: research
Eimeria tenella pyrroline -5-carboxylate reductase is a secreted protein and involved in host cell invasion
In this study, the function and characteristics of the pyrroline-5-carboxylate reductase of Eimeria tenella (EtPYCR) protein were preliminary analyzed. The transcription and translation level were analyzed by using qPCR and Western blot. The results showed that the mRNA transcription and translation levels of EtPYCR were higher in unsporulated oocysts (UO) and second generation merozoites (Mrz) than that in sporulated oocysts (SO) and sporozoites. Enzyme activity showed that the enzyme activity of EtPYCR was also higher in the UO and Mrz than that in the SO and sporozoites. Immunofluorescence localization showed EtPYCR was...
Source: Experimental Parasitology - February 9, 2024 Category: Parasitology Authors: Shanshan Liang Shunhai Zhu Qingjie Wang Qiping Zhao Hui Dong Bing Huang Yu Yu Hongyu Han Source Type: research
Efficacy of Lactobacillus taiwanensis S29 and Lactiplantibacillus plantarum S27 against tapeworm infection in Swiss Albino rats
Exp Parasitol. 2024 Feb 7;259:108715. doi: 10.1016/j.exppara.2024.108715. Online ahead of print.ABSTRACTHymenolepis diminuta a zoonotic tapeworm infection in human remains an important cestode model for anthelmintic study as it display common clinical symptoms like other adult human tapeworms during heavy infestation. The use of Lactobacillus as a probiotic is an alternative to drugs which have increased in research and usage considerably during the last decade. The present study aims to determine the anthelmintic efficacy of two probiotics, L. taiwanensis strain S29 and L. plantarum strain S27 against H. diminuta in infec...
Source: Experimental Parasitology - February 9, 2024 Category: Parasitology Authors: Sudeshna Mandal Chandrani Mondal Sinchan Ghosh Samiparna Saha Mou Singha Ray Larisha M Lyndem Source Type: research
Nematicidal activity of paucimannose-type glycoconjugates from acacia honey
Exp Parasitol. 2024 Feb 7;259:108707. doi: 10.1016/j.exppara.2024.108707. Online ahead of print.ABSTRACTNatural honey contains glycoconjugates as minor components. We characterized acacia honey glycoconjugates with molecular masses in the range of 2-5 kDa. The glycoconjugates were separated by RP-HPLC into three peaks (termed RP-2-5 k-I, RP-2-5 k-II, and RP-2-5 k-III) which demonstrated paralyzing effects on the model nematode C. elegans (ED50 of 50 ng glycoconjugates/μL). To examine molecular mechanisms underlying the nematicidal effects of honey glycoconjugates, expressional analyses of genes that are essential for the ...
Source: Experimental Parasitology - February 9, 2024 Category: Parasitology Authors: Bushra Bilal M Kamran Azim Source Type: research
Diurnal variation of the chemical composition and insecticidal activity of essential oils of three aromatic plants of the Togolese flora against the dengue vector Aedesaegypti
Exp Parasitol. 2024 Feb 5:108708. doi: 10.1016/j.exppara.2024.108708. Online ahead of print.ABSTRACTThe present reported work deals with the ability of Togolese plants' essential oils (EOs) to act as repellents for Aedes aegypti mosquitoes in order to use them as personal protective requirements or actions against mosquito bites and therefore to drastically reduce the risk of contracting dengue or yellow fever. EOs studied here were extracted from dry leaves of Ageratum conyzoides L., Eucalyptus citriodora Hook, and Lantana camara Linn, three plants that were collected at different daytimes (7 a.m., 1 p.m., and 7 p.m.) at ...
Source: Experimental Parasitology - February 7, 2024 Category: Parasitology Authors: Mou ïnatou Yeroukiriki Kafui Kpegba Koffi M Ahadji-Dabla Isma ël Ousseini Nafiou Kodjo Selom Evenamede Salom é D S Kpoviessi Catherine Malhiac Ata Martin Lawson Source Type: research
Changes in splenic tissue and immune response profile of Schistosoma mansoni infected mice submitted to chronic ethanol intake
Exp Parasitol. 2024 Feb 1;259:108706. doi: 10.1016/j.exppara.2024.108706. Online ahead of print.ABSTRACTIn Schistosoma mansoni infection, the spleen is one of the organs affected, causing its enlargement (splenomegaly). Intake of ethanol through alcoholic beverages can cause spleen atrophy and interfere with immune activity. To gain knowledge of this association on the spleen and on the immune response profile, male mice were used as an experimental model. These animals were divided into four groups: C. control; EC. uninfected/ethanol gavage; I. infected; and IE. infected/ethanol gavage. Groups I and IE were infected with ...
Source: Experimental Parasitology - February 3, 2024 Category: Parasitology Authors: Aline Aparecida da Rosa Luciana Brand ão-Bezerra Christiane Leal Corr êa Silvia Amaral G Da-Silva Luciana Silva Rodrigues Jos é Roberto Machado-Silva Renata Heisler Neves Source Type: research
Chemical composition and mosquitocidal properties of essential oil from Indian indigenous plants Ocimum tenuiflorum L. and Ocimum americanum L. against three vector mosquitoes
Exp Parasitol. 2024 Jan 30;258:108709. doi: 10.1016/j.exppara.2024.108709. Online ahead of print.ABSTRACTMosquitoes stand out as the most perilous and impactful vectors on a global scale, transmitting a multitude of infectious diseases to both humans and other animals. The primary objective of the current research was to assess the effectiveness of EOs from Ocimum tenuiflorum L. and Ocimum americanum L. in controlling Anopheles stephensi Liston. Culex quinquefasciatus Say and Aedes aegypti L. mosquitoes. The larvae, pupae and eggs of the mosquitoes were exposed to four different concentrations (6.25-50 ppm). The tested EOs...
Source: Experimental Parasitology - February 1, 2024 Category: Parasitology Authors: Kamaraj Ragavendran Jeyaraj Selvakumaran Mariappan Muthukanagavel Naiyf S Alharbi Muthu Thiruvengadam Subramanian Mutheeswaran Savarimuthu Ignacimuthu Pathalam Ganesan Source Type: research
Chemical compounds, antioxidant and scolicidal potencies of Thymus fontanesii essential oil
Exp Parasitol. 2024 Feb;257:108699. doi: 10.1016/j.exppara.2024.108699. Epub 2024 Jan 9.ABSTRACTMedicinal plants have become a source of new drugs to treat various diseases due to their low cost, few side effects, and wide accessibility. Among these, essential oils are promising natural compounds for use in scolicidal activity. The targets of our current work were to assess the chemical composition, antioxidant and protoscolicidal activities of Thymus fontanesii essential oil. The essential oil was extracted by hydrodistillation, and chemical compounds were quantified by GC-MS/GC-FID. The in vitro antioxidant activity was ...
Source: Experimental Parasitology - January 10, 2024 Category: Parasitology Authors: Sidi Mohammed Ammar Selles Belkacem Tahar Belhamiti Mokhtaria Kouidri Amar Ait Amrane Yamina Kadari Zohra Kaddour Souad Kabrit Source Type: research
