Microencapsulation of Bacillus thuringiensis strains for the control of Aedes aegypti
In this study, we investigated the microencapsulation of two strains of the entomopathogenic bacteria Bacillus thuringiensis (B. thuringiensis) (BtMA-750 and BtMA-1114), which are biopesticides of high toxicity for the mosquito vector Aedes aegypti. The encapsulation of different concentrations of microorganisms in starch microparticles was evaluated, and the inverse suspension polymerization technique was explored. It was possible to observe that the higher amounts of the biopesticide caused a slight decrease in the diameter of the particles; however, even when encapsulated, the biopesticide still presents an average diam...
Source: Experimental Parasitology - November 13, 2023 Category: Parasitology Authors: Juliete L Viana Joelma S da Silva Gabriela C de Mattos Martina C C Pinto Luciana da S Dutra Larissa L de A Carvalho Jos é Carlos C da S Pinto Val éria Cristina S Pinheiro Rosemary A Roque Source Type: research
Microencapsulation of Bacillus thuringiensis strains for the control of Aedes aegypti
In this study, we investigated the microencapsulation of two strains of the entomopathogenic bacteria Bacillus thuringiensis (B. thuringiensis) (BtMA-750 and BtMA-1114), which are biopesticides of high toxicity for the mosquito vector Aedes aegypti. The encapsulation of different concentrations of microorganisms in starch microparticles was evaluated, and the inverse suspension polymerization technique was explored. It was possible to observe that the higher amounts of the biopesticide caused a slight decrease in the diameter of the particles; however, even when encapsulated, the biopesticide still presents an average diam...
Source: Experimental Parasitology - November 13, 2023 Category: Parasitology Authors: Juliete L Viana Joelma S da Silva Gabriela C de Mattos Martina C C Pinto Luciana da S Dutra Larissa L de A Carvalho Jos é Carlos C da S Pinto Val éria Cristina S Pinheiro Rosemary A Roque Source Type: research
Microencapsulation of Bacillus thuringiensis strains for the control of Aedes aegypti
In this study, we investigated the microencapsulation of two strains of the entomopathogenic bacteria Bacillus thuringiensis (B. thuringiensis) (BtMA-750 and BtMA-1114), which are biopesticides of high toxicity for the mosquito vector Aedes aegypti. The encapsulation of different concentrations of microorganisms in starch microparticles was evaluated, and the inverse suspension polymerization technique was explored. It was possible to observe that the higher amounts of the biopesticide caused a slight decrease in the diameter of the particles; however, even when encapsulated, the biopesticide still presents an average diam...
Source: Experimental Parasitology - November 13, 2023 Category: Parasitology Authors: Juliete L Viana Joelma S da Silva Gabriela C de Mattos Martina C C Pinto Luciana da S Dutra Larissa L de A Carvalho Jos é Carlos C da S Pinto Val éria Cristina S Pinheiro Rosemary A Roque Source Type: research
The common bed bug Cimex lectularius synthesizes hemozoin as an essential defense against the toxic effects of heme
Exp Parasitol. 2023 Nov 9:108653. doi: 10.1016/j.exppara.2023.108653. Online ahead of print.ABSTRACTThe common bed bug Cimex lectularius (Linnaeus 1758) is an ectoparasite that feeds preferably on human blood, being considered an important public health issue. Blood-feeding is a challenging process for hematophagous organisms, and one of the inherent risks with this kind of diet is the liberation of high doses of free heme after the digestion of hemoglobin. In order to deal with this potent cytotoxic agent, such organisms have acquired different defense mechanisms. Here, we use UV-visible spectrophotometry and infrared spe...
Source: Experimental Parasitology - November 11, 2023 Category: Parasitology Authors: Vladimir Fazito do Vale Brenda Hevillin Rocha Simtob Luccas Gabriel Ferreira Malta Ezequias Pessoa de Siqueira Source Type: research
The common bed bug Cimex lectularius synthesizes hemozoin as an essential defense against the toxic effects of heme
Exp Parasitol. 2023 Nov 9:108653. doi: 10.1016/j.exppara.2023.108653. Online ahead of print.ABSTRACTThe common bed bug Cimex lectularius (Linnaeus 1758) is an ectoparasite that feeds preferably on human blood, being considered an important public health issue. Blood-feeding is a challenging process for hematophagous organisms, and one of the inherent risks with this kind of diet is the liberation of high doses of free heme after the digestion of hemoglobin. In order to deal with this potent cytotoxic agent, such organisms have acquired different defense mechanisms. Here, we use UV-visible spectrophotometry and infrared spe...
Source: Experimental Parasitology - November 11, 2023 Category: Parasitology Authors: Vladimir Fazito do Vale Brenda Hevillin Rocha Simtob Luccas Gabriel Ferreira Malta Ezequias Pessoa de Siqueira Source Type: research
TLR2, TLR3, TLR4, TLR9 and TLR11 expression on effector CD4 < sup > + < /sup > T-cell subsets in Leishmania donovani infection
We report: CD4+t-bet+ T-cells are frequent in TLR2LowTLR3HighTLR4Low subpopulation, CD4+GATA3+ T-cells are frequent within the cells with intermediate expression of TLR2, TLR3, TLR4 and TLR11, CD4+FoxP3+ T-cells in TLR2HighTLR3High cells whereas CD4+RORγt + T-cells are frequent in TLR2LowTLR3LowTLR4LowTLR11Low cells. CD4+ effector T-cell subsets may therefore show association with TLRs- TLR3, in particular-expression. In Leishmania donovani infection in BALB/c mice, TLR3 expression on both CD4+ and CD8+ T-cells is reduced. Poly-I:C, a TLR3 ligand, do not have any distinctive effects on the CD4+ effector T-cell subsets. Th...
Source: Experimental Parasitology - November 10, 2023 Category: Parasitology Authors: Ashok Patidar Divanshu Shukla Neelam Bodhale Bhaskar Saha Source Type: research
Ribonucleotide reductase as a therapeutic target for drug repurposing as anthelmintics
Exp Parasitol. 2023 Nov 8:108641. doi: 10.1016/j.exppara.2023.108641. Online ahead of print.ABSTRACTVisceral cestodiases, like echinococcoses and cysticercoses, are zoonoses of worldwide distribution and are responsible for public health problems in many countries, especially in underdeveloped regions. Current treatments have low efficiency and there are few drugs currently in use for chemotherapy, making the development of new anthelmintics an urgent matter. The nucleotide salvage pathways are the only ones available for nucleotide synthesis in cestodes and other parasitic helminths, and, here, we used in silico approache...
Source: Experimental Parasitology - November 10, 2023 Category: Parasitology Authors: Marcelo Pasa Panesso Martin Cancela Renato Kulakowski Cor á J éssica Andrade Paes Gabriel Prado Paludo Henrique Bunselmeyer Ferreira Source Type: research
Evaluation of synergism in essential oils against the cattle tick Rhipicephalus microplus in Burkina Faso
Exp Parasitol. 2023 Nov 8:108643. doi: 10.1016/j.exppara.2023.108643. Online ahead of print.ABSTRACTThe cattle tick Rhipicephalus microplus affects animal production economically by reducing weight gain and milk production and causing diseases, such as babesiosis and anaplasmosis. Using synthetic acaricides to reduce their incidence has caused the emergence of resistant tick populations. The present study aimed to assess the in vitro acaricidal activity of combinations of essential oils (EOs) from Ocimum americanum, Ocimum gratissimum, and Lippia multiflora against R. microplus larvae. In fact, numerous biological properti...
Source: Experimental Parasitology - November 10, 2023 Category: Parasitology Authors: Anass Coulibaly Abel S Biguezoton Delphine M Hema Firmin F Dah Ignace Sawadogo R émy K Bationo Moussa Compaore Martin Kiendrebeogo Roger C H Nebie Source Type: research
Genetic diversity and haplotypes of Echinococcus granulosus isolated from cattle and buffaloes and first report of E. ortleppi (G5) in buffaloes in Pakistan based on mitochondrial cytochrome c oxidase subunit-1 gene (mt-CO1) markers
Exp Parasitol. 2023 Nov 7:108648. doi: 10.1016/j.exppara.2023.108648. Online ahead of print.ABSTRACTCystic echinococcosis (CE) is a parasitic disease, caused by larval stage of Echinococcus granulosus sensu lato, one of the most important and neglected zoonotic diseases. Although the disease is endemic in neighboring countries, information regarding genetic diversity of E. granulosus s.l. is scarce in Pakistan. Therefore, the main purpose of this report was to contribute in molecular epidemiology and to find genetic diversity and haplotypes of E. granulosus s.l. in cattle and buffalo isolates. To identify species circulati...
Source: Experimental Parasitology - November 9, 2023 Category: Parasitology Authors: Huma Khan Figen Celik Sami Simsek Majid Fasihi Harandi Haroon Ahmed Source Type: research
Evaluation of Brown and red seaweeds-extracts as a novel larvicidal agent against the deadly human diseases-vectors, Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus
In this study, we assessed the larvicidal activity of commonly found seaweeds, including Padina gymnospora, P. pavonica, Gracilaria crassa, Amphiroa fragilissima, and Spatoglossum marginatum, against these mosquito vectors. Our findings indicate that extracts from P. gymnospora Ethyl Acetate (PgEA), P. pavonica Hexane (PpH), and A. fragilissima Ethyl Acetate (AfEA) displayed the highest larval mortality rates for A. stephensi, with LC50 values of 10.51, 12.43, and 6.43 μg/mL, respectively. Additionally, the PgEA extract from P. gymnospora exhibited the highest mortality rate for A. aegypti, with an LC50 of 27.0 μg/mL, wh...
Source: Experimental Parasitology - November 9, 2023 Category: Parasitology Authors: Annamalai Aravinth Sundaramoorthy Dhanasundaram Pachiappan Perumal Chinnaperumal Kamaraj Safir Ullah Khan Amir Ali Chinnasamy Ragavendran Vadivel Amutha Rajendran Rajaram Perumal Santhanam Juan Pedro Luna-Arias Zia-Ur-Rehman Mashwani Source Type: research
Genetic diversity and haplotypes of Echinococcus granulosus isolated from cattle and buffaloes and first report of E. ortleppi (G5) in buffaloes in Pakistan based on mitochondrial cytochrome c oxidase subunit-1 gene (mt-CO1) markers
Exp Parasitol. 2023 Nov 7:108648. doi: 10.1016/j.exppara.2023.108648. Online ahead of print.ABSTRACTCystic echinococcosis (CE) is a parasitic disease, caused by larval stage of Echinococcus granulosus sensu lato, one of the most important and neglected zoonotic diseases. Although the disease is endemic in neighboring countries, information regarding genetic diversity of E. granulosus s.l. is scarce in Pakistan. Therefore, the main purpose of this report was to contribute in molecular epidemiology and to find genetic diversity and haplotypes of E. granulosus s.l. in cattle and buffalo isolates. To identify species circulati...
Source: Experimental Parasitology - November 9, 2023 Category: Parasitology Authors: Huma Khan Figen Celik Sami Simsek Majid Fasihi Harandi Haroon Ahmed Source Type: research
Evaluation of Brown and red seaweeds-extracts as a novel larvicidal agent against the deadly human diseases-vectors, Anopheles stephensi, Aedes aegypti and Culex quinquefasciatus
In this study, we assessed the larvicidal activity of commonly found seaweeds, including Padina gymnospora, P. pavonica, Gracilaria crassa, Amphiroa fragilissima, and Spatoglossum marginatum, against these mosquito vectors. Our findings indicate that extracts from P. gymnospora Ethyl Acetate (PgEA), P. pavonica Hexane (PpH), and A. fragilissima Ethyl Acetate (AfEA) displayed the highest larval mortality rates for A. stephensi, with LC50 values of 10.51, 12.43, and 6.43 μg/mL, respectively. Additionally, the PgEA extract from P. gymnospora exhibited the highest mortality rate for A. aegypti, with an LC50 of 27.0 μg/mL, wh...
Source: Experimental Parasitology - November 9, 2023 Category: Parasitology Authors: Annamalai Aravinth Sundaramoorthy Dhanasundaram Pachiappan Perumal Chinnaperumal Kamaraj Safir Ullah Khan Amir Ali Chinnasamy Ragavendran Vadivel Amutha Rajendran Rajaram Perumal Santhanam Juan Pedro Luna-Arias Zia-Ur-Rehman Mashwani Source Type: research
Development of a novel molecular tool to study molecular ecology of Ornithomya (Hippoboscidae) avian louse flies
Exp Parasitol. 2023 Nov 6:108652. doi: 10.1016/j.exppara.2023.108652. Online ahead of print.ABSTRACTLouse flies (Diptera: Hippoboscidae) are obligatory hematophagous ectoparasites of birds and mammals. These widely distributed parasitic flies may have a significant impact on wild and farm animals by feeding on their blood and transmitting bloodborne pathogens. However, despite their ecological importance, louse flies are clearly underrepresented in host-parasite research and, implementation of genetic approaches in this group is generally hampered by lacking molecular tools. In addition, louse flies that parasitize long-di...
Source: Experimental Parasitology - November 8, 2023 Category: Parasitology Authors: Aleksandra Janiszewska Maciej Bartos Łukasz Trębicki Magdalena Remisiewicz Grzegorz Cierlik Piotr Minias Tomasz Rewicz Source Type: research
Nanoliposomal Trachyspermum ammi (L) sprague essential oil for effective control of malaria mosquito larvae, Anopheles stephensi Liston
Exp Parasitol. 2023 Nov 6:108644. doi: 10.1016/j.exppara.2023.108644. Online ahead of print.ABSTRACTControlling mosquito vectors at immature stages using larvicides is a practical strategy to stave off mosquito-borne diseases such as malaria. Developing nanoliposomes bearing essential oil is a promising approach to improving the efficacy and stability of EOs-derived larvicides. The main aim of this investigation was to assess the efficacy of nanoliposome containing Trachyspermum ammi L. EO (TAEO-NL) as a new potential formulation to control Anopheles stephensi Liston (Diptera, Culicidae) mosquito larvae. The chemical const...
Source: Experimental Parasitology - November 8, 2023 Category: Parasitology Authors: Z Hosseinizadeh M Osanloo H Alipour R Heiran M Shahriari-Namadi M D Moemenbellah-Fard Source Type: research
The branched chain amino acids (BCAAs) modulate the development of the intra-cellular stages of Trypanosoma cruzi
In this study, we investigated further the role of branched-chain amino acids (BCAAs) as negative modulators of parasite differentiation and infection capability in vitro. We found that BCAAs can down-regulate metacyclogenesis, inhibiting Pro-dependent differentiation. Furthermore, we evaluated the ability of all three BCAAs to influence the differentiation of intracellular stages and found that they could modulate the release of trypomastigotes from infected host cells. These findings suggest that BCAAs may have an important role in the complex life cycle of T. cruzi. Thus, enzymes of their metabolism and other interactin...
Source: Experimental Parasitology - November 8, 2023 Category: Parasitology Authors: L N Rapado J F Nascimento N C Manchola F S Damasceno R W Achjian A M Silber Source Type: research
