A miRNA-7704/IL2RB/AKT feedback loop regulates tumorigenesis and chemoresistance in ovarian cancer
In this study, we found that miR-7704 inhibited the proliferation and promoted cisplatin sensitivity of ovarian cancer cells in vitro and in vivo. Moreover, ectopic expression of miR-7704 had the same effect as IL2RB knockdown. Further mechanistic studies revealed that miR-7704 played an inhibitory role by regulating IL2RB expression to inactivate the AKT signaling pathway. Furthermore, IL2RB reversed the miR-7704 mediated resistance to cisplatin in ovarian cancer. Based on these findings, miR-7704 and IL2RB show the potential as novel therapeutic targets for ovarian cancer.PMID:38565343 | DOI:10.1016/j.yexcr.2024.114012 (...
Source: Experimental Cell Research - April 2, 2024 Category: Cytology Authors: Xuan Meng Xiaoqing Liang Shengjie Yang Dongsheng Wu Xinghe Wang Source Type: research
A miRNA-7704/IL2RB/AKT feedback loop regulates tumorigenesis and chemoresistance in ovarian cancer
In this study, we found that miR-7704 inhibited the proliferation and promoted cisplatin sensitivity of ovarian cancer cells in vitro and in vivo. Moreover, ectopic expression of miR-7704 had the same effect as IL2RB knockdown. Further mechanistic studies revealed that miR-7704 played an inhibitory role by regulating IL2RB expression to inactivate the AKT signaling pathway. Furthermore, IL2RB reversed the miR-7704 mediated resistance to cisplatin in ovarian cancer. Based on these findings, miR-7704 and IL2RB show the potential as novel therapeutic targets for ovarian cancer.PMID:38565343 | DOI:10.1016/j.yexcr.2024.114012 (...
Source: Experimental Cell Research - April 2, 2024 Category: Cytology Authors: Xuan Meng Xiaoqing Liang Shengjie Yang Dongsheng Wu Xinghe Wang Source Type: research
The CNS microenvironment promotes leukemia cell survival by disrupting tumor suppression and cell cycle regulation in pediatric T-cell acute lymphoblastic leukemia
Exp Cell Res. 2024 Mar 30:114015. doi: 10.1016/j.yexcr.2024.114015. Online ahead of print.ABSTRACTA major obstacle in improving survival in pediatric T-cell acute lymphoblastic leukemia is understanding how to predict and treat leukemia relapse in the CNS. Leukemia cells are capable of infiltrating and residing within the CNS, primarily the leptomeninges, where they interact with the microenvironment and remain sheltered from systemic treatment. These cells can survive in the CNS, by hijacking the microenvironment and disrupting normal functions, thus promoting malignant transformation. While the protective effects of the ...
Source: Experimental Cell Research - April 1, 2024 Category: Cytology Authors: Sabina Enlund Indranil Sinha Christina Neofytou Amanda Ramilo Amor Konstantinos Papadakis Anna Nilsson Qingfei Jiang Ola Hermanson Frida Holm Source Type: research
The CNS microenvironment promotes leukemia cell survival by disrupting tumor suppression and cell cycle regulation in pediatric T-cell acute lymphoblastic leukemia
Exp Cell Res. 2024 Mar 30:114015. doi: 10.1016/j.yexcr.2024.114015. Online ahead of print.ABSTRACTA major obstacle in improving survival in pediatric T-cell acute lymphoblastic leukemia is understanding how to predict and treat leukemia relapse in the CNS. Leukemia cells are capable of infiltrating and residing within the CNS, primarily the leptomeninges, where they interact with the microenvironment and remain sheltered from systemic treatment. These cells can survive in the CNS, by hijacking the microenvironment and disrupting normal functions, thus promoting malignant transformation. While the protective effects of the ...
Source: Experimental Cell Research - April 1, 2024 Category: Cytology Authors: Sabina Enlund Indranil Sinha Christina Neofytou Amanda Ramilo Amor Konstantinos Papadakis Anna Nilsson Qingfei Jiang Ola Hermanson Frida Holm Source Type: research
Alterations of UHRF family Expression and was regulated by High Risk Type HPV16 in Uterine Cervical Cancer
In this study, we analyzed the UHRF family expression in cervical cancers, and it's regulation by human papillomavirus (HPV). Western blotting was performed to analyze protein expression in cervical cancer cell lines. Immunohistochemical analysis were used to investigate the expression of UHRF family and MIB-1 in cervical cancer tissues. Transfection were done for analyze the relationship between UHRF family and HPVs. We showed that NIRF expression was decreased and ICBP90 expression was increased in cervical cancers compared to normal counterparts. Western blotting also showed that NIRF expression was quite low levels, bu...
Source: Experimental Cell Research - March 31, 2024 Category: Cytology Authors: Masafumi Yoshimoto Aoi Tokuda Ayami Eguchi Yoshihiro Nozawa Tsutomu Mori Yuji Yaginuma Source Type: research
Truncated variants of thyroid hormone receptor beta display disease-inflicting malfunctioning at cellular level
In this study, we investigated disease-inflicting truncated variants of THRβ using in-silico analysis and cell-based assays. We examined the THRβ truncated variants on multiple test parameters, including subcellular localization, ligand-receptor interactions, transcriptional functions, interaction with heterodimeric partner RXR, and receptor-chromatin interactions. Moreover, molecular dynamic simulation approaches predicted that shortened THRβ-LBD due to point mutations contributes proportionally to the loss of structural integrity and receptor stability. Deviant subcellular localization and compromised transcriptional ...
Source: Experimental Cell Research - March 30, 2024 Category: Cytology Authors: Ghausiya Rehman Jyoti Kashyap Amit Kumar Srivastav Sheeba Rizvi Umesh Kumar Rakesh K Tyagi Source Type: research
In vivo tracking of mesenchymal stem cell dynamics and therapeutics in LPS-induced acute lung injury models
This study emphasizes the importance of the "lung window" for live imaging of the cellular behavior of MSCs by vein injection. Moreover, our results revealed that the upregulation of vascular cell adhesion molecule 1 (VCAM1) in endothelial cells post-inflammatory injury could enhance MSC retention in the lung, further ameliorating acute lung injury. In summary, intravital microscopy imaging provides a practical method to investigate the therapeutic effects of MSCs in acute lung injury.PMID:38555014 | DOI:10.1016/j.yexcr.2024.114013 (Source: Experimental Cell Research)
Source: Experimental Cell Research - March 30, 2024 Category: Cytology Authors: Chen Wang Kamal Hezam Enze Fu Kai Pan Yue Liu Zongjin Li Source Type: research
Truncated variants of thyroid hormone receptor beta display disease-inflicting malfunctioning at cellular level
In this study, we investigated disease-inflicting truncated variants of THRβ using in-silico analysis and cell-based assays. We examined the THRβ truncated variants on multiple test parameters, including subcellular localization, ligand-receptor interactions, transcriptional functions, interaction with heterodimeric partner RXR, and receptor-chromatin interactions. Moreover, molecular dynamic simulation approaches predicted that shortened THRβ-LBD due to point mutations contributes proportionally to the loss of structural integrity and receptor stability. Deviant subcellular localization and compromised transcriptional ...
Source: Experimental Cell Research - March 30, 2024 Category: Cytology Authors: Ghausiya Rehman Jyoti Kashyap Amit Kumar Srivastav Sheeba Rizvi Umesh Kumar Rakesh K Tyagi Source Type: research
In vivo tracking of mesenchymal stem cell dynamics and therapeutics in LPS-induced acute lung injury models
This study emphasizes the importance of the "lung window" for live imaging of the cellular behavior of MSCs by vein injection. Moreover, our results revealed that the upregulation of vascular cell adhesion molecule 1 (VCAM1) in endothelial cells post-inflammatory injury could enhance MSC retention in the lung, further ameliorating acute lung injury. In summary, intravital microscopy imaging provides a practical method to investigate the therapeutic effects of MSCs in acute lung injury.PMID:38555014 | DOI:10.1016/j.yexcr.2024.114013 (Source: Experimental Cell Research)
Source: Experimental Cell Research - March 30, 2024 Category: Cytology Authors: Chen Wang Kamal Hezam Enze Fu Kai Pan Yue Liu Zongjin Li Source Type: research
Dysregulated MicroRNAs and long non-coding RNAs associated with extracellular matrix stiffness
Exp Cell Res. 2024 Mar 26:114014. doi: 10.1016/j.yexcr.2024.114014. Online ahead of print.ABSTRACTExtracellular matrix (ECM) stiffness regulates development and homeostasis in vivo and affects both physiological and pathological processes. A variety of studies have demonstrated that mRNAs, such as Piezo1, integrin β1, and Yes-associated protein (YAP)/tafazzin (TAZ), can sense the mechanical signals induced by ECM stiffness and transmit them from the extracellular space into the cytoplasm. Non-coding RNAs (ncRNAs), such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), have been reported to play important roles in ...
Source: Experimental Cell Research - March 28, 2024 Category: Cytology Authors: Huimin Qiu Yi Fu Zhinan Guo Xinjia Zhang Xinyue Wang Hailong Wu Source Type: research
Dysregulated MicroRNAs and long non-coding RNAs associated with extracellular matrix stiffness
Exp Cell Res. 2024 Mar 26:114014. doi: 10.1016/j.yexcr.2024.114014. Online ahead of print.ABSTRACTExtracellular matrix (ECM) stiffness regulates development and homeostasis in vivo and affects both physiological and pathological processes. A variety of studies have demonstrated that mRNAs, such as Piezo1, integrin β1, and Yes-associated protein (YAP)/tafazzin (TAZ), can sense the mechanical signals induced by ECM stiffness and transmit them from the extracellular space into the cytoplasm. Non-coding RNAs (ncRNAs), such as microRNAs (miRNAs) and long non-coding RNAs (lncRNAs), have been reported to play important roles in ...
Source: Experimental Cell Research - March 28, 2024 Category: Cytology Authors: Huimin Qiu Yi Fu Zhinan Guo Xinjia Zhang Xinyue Wang Hailong Wu Source Type: research
Young human plasma-derived extracellular vesicles rescue and reactivate IL-1 β and TNF-α treated chondrocytes
In this study, we first evaluated EVs derived from young human plasma (YEVs) and EVs derived from old human plasma (OEVs) in an in vitro experiment using chondrocytes. The results revealed that YEVs effectively stimulated chondrocyte proliferation and migration, while OEVs from old plasma did not exhibit a similar effect. Given that OA represents a more complex inflammatory microenvironment, we further determine whether the benefits of YEVs on chondrocytes can be maintained in this context. Our findings indicate that YEVs have the ability to positively regulate chondrocyte function and protect them against apoptosis induce...
Source: Experimental Cell Research - March 27, 2024 Category: Cytology Authors: Rongjie Wu Yu Xie Yujie Peng Xiaohu Wu Yuanchen Ma Feng-Juan Lyu Qiujian Zheng Zhantao Deng Source Type: research
DNA damage-regulated autophagy modulator 1 prevents glioblastoma cells proliferation by regulating lysosomal function and autophagic flux stability
Exp Cell Res. 2024 Mar 25:114016. doi: 10.1016/j.yexcr.2024.114016. Online ahead of print.ABSTRACTGlioblastoma (GBM) is the most aggressive and life-threatening brain tumor, characterized by its highly malignant and recurrent nature. DNA damage-regulated autophagy modulator 1 (DRAM-1) is a p53 target gene encoding a lysosomal protein that induces macro-autophagy and damage-induced programmed cell death in tumor growth. However, the precise mechanisms underlying how DRAM-1 affects tumor cell proliferation through regulation of lysosomal function and autophagic flux stability remain incompletely understood. We found that DRA...
Source: Experimental Cell Research - March 27, 2024 Category: Cytology Authors: Hongqiang Zhang Lan Luan Xinyu Li Xu Sun Kang Yang Source Type: research
Young human plasma-derived extracellular vesicles rescue and reactivate IL-1 β and TNF-α treated chondrocytes
In this study, we first evaluated EVs derived from young human plasma (YEVs) and EVs derived from old human plasma (OEVs) in an in vitro experiment using chondrocytes. The results revealed that YEVs effectively stimulated chondrocyte proliferation and migration, while OEVs from old plasma did not exhibit a similar effect. Given that OA represents a more complex inflammatory microenvironment, we further determine whether the benefits of YEVs on chondrocytes can be maintained in this context. Our findings indicate that YEVs have the ability to positively regulate chondrocyte function and protect them against apoptosis induce...
Source: Experimental Cell Research - March 27, 2024 Category: Cytology Authors: Rongjie Wu Yu Xie Yujie Peng Xiaohu Wu Yuanchen Ma Feng-Juan Lyu Qiujian Zheng Zhantao Deng Source Type: research
DNA damage-regulated autophagy modulator 1 prevents glioblastoma cells proliferation by regulating lysosomal function and autophagic flux stability
Exp Cell Res. 2024 Mar 25:114016. doi: 10.1016/j.yexcr.2024.114016. Online ahead of print.ABSTRACTGlioblastoma (GBM) is the most aggressive and life-threatening brain tumor, characterized by its highly malignant and recurrent nature. DNA damage-regulated autophagy modulator 1 (DRAM-1) is a p53 target gene encoding a lysosomal protein that induces macro-autophagy and damage-induced programmed cell death in tumor growth. However, the precise mechanisms underlying how DRAM-1 affects tumor cell proliferation through regulation of lysosomal function and autophagic flux stability remain incompletely understood. We found that DRA...
Source: Experimental Cell Research - March 27, 2024 Category: Cytology Authors: Hongqiang Zhang Lan Luan Xinyu Li Xu Sun Kang Yang Source Type: research
