GPx1 promotes hypertrophic differentiation of chondrocytes through modulation of akt signaling in a non-monotonic manner
Exp Cell Res. 2023 Oct 25:113824. doi: 10.1016/j.yexcr.2023.113824. Online ahead of print.NO ABSTRACTPMID:37890608 | DOI:10.1016/j.yexcr.2023.113824 (Source: Experimental Cell Research)
Source: Experimental Cell Research - October 27, 2023 Category: Cytology Authors: Sijia Mi Jinhong Zhang Mengyao Sun Xinyu Huo Yaqi Lyu Frank Beier Shemin Lu Jidong Yan Source Type: research

Corrigendum to "iPS-derived neural stem cells for disease modeling and evaluation of therapeutics for mucopolysaccharidosis type II" [Exp. Cell Res. 412, Issue 1, 1 March 2022, 113007]
Exp Cell Res. 2023 Oct 24;433(2):113808. doi: 10.1016/j.yexcr.2023.113808. Online ahead of print.NO ABSTRACTPMID:37883891 | DOI:10.1016/j.yexcr.2023.113808 (Source: Experimental Cell Research)
Source: Experimental Cell Research - October 26, 2023 Category: Cytology Authors: Junjie Hong Yu-Shan Cheng Shu Yang Manju Swaroop Miao Xu Jeanette Beers Jizhong Zou Wenwei Huang Juan J Marugan Xiujun Cai Wei Zheng Source Type: research

Corrigendum to "iPS-derived neural stem cells for disease modeling and evaluation of therapeutics for mucopolysaccharidosis type II" [Exp. Cell Res. 412, Issue 1, 1 March 2022, 113007]
Exp Cell Res. 2023 Oct 24;433(2):113808. doi: 10.1016/j.yexcr.2023.113808. Online ahead of print.NO ABSTRACTPMID:37883891 | DOI:10.1016/j.yexcr.2023.113808 (Source: Experimental Cell Research)
Source: Experimental Cell Research - October 26, 2023 Category: Cytology Authors: Junjie Hong Yu-Shan Cheng Shu Yang Manju Swaroop Miao Xu Jeanette Beers Jizhong Zou Wenwei Huang Juan J Marugan Xiujun Cai Wei Zheng Source Type: research

Rapamycin promotes hematoma resorption and enhances endothelial cell function by suppressing the mTOR/STAT3 signaling in chronic subdural hematoma
This study focuses on the functions of rapamycin and its related molecular mechanisms in CSDH management. A rat model of CSDH was induced, which developed significant hematoma on day 5 after operation. The rats were treated with rapamycin or atorvastatin, a drug with known effect on hematoma alleviation, or treated with rapamycin and atorvastatin in combination. The atorvastatin or rapamycin treatment reduced the hematoma development, blood-brain barrier permeability, neurological dysfunction in CSDH rats, and the combination treatment showed more pronounced effects. Human brain microvascular endothelial cells hCMEC/D3 wer...
Source: Experimental Cell Research - October 25, 2023 Category: Cytology Authors: Jie Shen Yile Zhang Xiaoqiang Wu Source Type: research

Myotube growth is associated with cancer-like metabolic reprogramming and is limited by phosphoglycerate dehydrogenase
Exp Cell Res. 2023 Oct 23:113820. doi: 10.1016/j.yexcr.2023.113820. Online ahead of print.ABSTRACTThe Warburg effect links growth and glycolysis in cancer. A key purpose of the Warburg effect is to generate glycolytic intermediates for anabolic reactions, such as nucleotides → RNA/DNA and amino acids → protein synthesis. The aim of this study was to investigate whether a similar 'glycolysis-for-anabolism' metabolic reprogramming also occurs in hypertrophying muscles. To interrogate this, we first induced C2C12 myotube hypertrophy with IGF-1. We then added 14C glucose to the differentiation medium and measured radioacti...
Source: Experimental Cell Research - October 25, 2023 Category: Cytology Authors: Lian E M Stadhouders Jonathon A B Smith Brendan M Gabriel Sander A J Verbrugge Tim D Hammersen Detmar Kolijn Ilse S P Vogel Abdalla D Mohamed Gerard M J de Wit Carla Offringa Willem M H Hoogaars Sebastian Gehlert Henning Wackerhage Richard T Jaspers Source Type: research

Rapamycin promotes hematoma resorption and enhances endothelial cell function by suppressing the mTOR/STAT3 signaling in chronic subdural hematoma
This study focuses on the functions of rapamycin and its related molecular mechanisms in CSDH management. A rat model of CSDH was induced, which developed significant hematoma on day 5 after operation. The rats were treated with rapamycin or atorvastatin, a drug with known effect on hematoma alleviation, or treated with rapamycin and atorvastatin in combination. The atorvastatin or rapamycin treatment reduced the hematoma development, blood-brain barrier permeability, neurological dysfunction in CSDH rats, and the combination treatment showed more pronounced effects. Human brain microvascular endothelial cells hCMEC/D3 wer...
Source: Experimental Cell Research - October 25, 2023 Category: Cytology Authors: Jie Shen Yile Zhang Xiaoqiang Wu Source Type: research

Myotube growth is associated with cancer-like metabolic reprogramming and is limited by phosphoglycerate dehydrogenase
Exp Cell Res. 2023 Oct 23:113820. doi: 10.1016/j.yexcr.2023.113820. Online ahead of print.ABSTRACTThe Warburg effect links growth and glycolysis in cancer. A key purpose of the Warburg effect is to generate glycolytic intermediates for anabolic reactions, such as nucleotides → RNA/DNA and amino acids → protein synthesis. The aim of this study was to investigate whether a similar 'glycolysis-for-anabolism' metabolic reprogramming also occurs in hypertrophying muscles. To interrogate this, we first induced C2C12 myotube hypertrophy with IGF-1. We then added 14C glucose to the differentiation medium and measured radioacti...
Source: Experimental Cell Research - October 25, 2023 Category: Cytology Authors: Lian E M Stadhouders Jonathon A B Smith Brendan M Gabriel Sander A J Verbrugge Tim D Hammersen Detmar Kolijn Ilse S P Vogel Abdalla D Mohamed Gerard M J de Wit Carla Offringa Willem M H Hoogaars Sebastian Gehlert Henning Wackerhage Richard T Jaspers Source Type: research

miR-212/132 attenuates OVA-induced airway inflammation by inhibiting mast cells activation through MRGPRX2 and ASAP1
In this study, the predicted pairing of miRNAs and MRGPRX2 (MRGPRB2) mRNAs was carried out by online databases and the function was verify using in vivo and in vitro experiments. Database prediction showed that miR-212/132 interact with MRGPRX2 and MRGPRB2. miR-212/132 mimics alleviated MRGPRB2 mRNA expression as well as pathology changes in lungs and AHR of mice with airway inflammation in vivo. The expression level of MRGPRB2 in the mice lungs after inhaled OVA was also decreased by miR-212/132 mimics. Meanwhile, miR-212/132 inhibited MCs degranulation and cytokines release triggered by C48/80 in vitro. Further, ASAP1 (A...
Source: Experimental Cell Research - October 24, 2023 Category: Cytology Authors: Yongjing Zhang Haoyun Bai Wen Zhang Jie Gao Chang Gao Tingting Deng Xinyu Liu Xiuzhen Sun Yun Liu Nan Wang Yuanyuan Wu Source Type: research

miR-212/132 attenuates OVA-induced airway inflammation by inhibiting mast cells activation through MRGPRX2 and ASAP1
In this study, the predicted pairing of miRNAs and MRGPRX2 (MRGPRB2) mRNAs was carried out by online databases and the function was verify using in vivo and in vitro experiments. Database prediction showed that miR-212/132 interact with MRGPRX2 and MRGPRB2. miR-212/132 mimics alleviated MRGPRB2 mRNA expression as well as pathology changes in lungs and AHR of mice with airway inflammation in vivo. The expression level of MRGPRB2 in the mice lungs after inhaled OVA was also decreased by miR-212/132 mimics. Meanwhile, miR-212/132 inhibited MCs degranulation and cytokines release triggered by C48/80 in vitro. Further, ASAP1 (A...
Source: Experimental Cell Research - October 24, 2023 Category: Cytology Authors: Yongjing Zhang Haoyun Bai Wen Zhang Jie Gao Chang Gao Tingting Deng Xinyu Liu Xiuzhen Sun Yun Liu Nan Wang Yuanyuan Wu Source Type: research

SENP1-mediated SUMOylation of SIRT1 affects glioma development through the NF- κB pathway
Exp Cell Res. 2023 Oct 20:113822. doi: 10.1016/j.yexcr.2023.113822. Online ahead of print.ABSTRACTGliomas are the most common primary brain tumors in adults. Although they exist in different malignant stages, most gliomas are clinically challenging because of their infiltrative growth patterns and inherent relapse tendency with increased malignancy. Epigenetic alterations have been suggested to be an important factor for glioma genesis. Using mRNA probe hybridization, we identified SUMO-specific protease 1 (SENP1) as the most significantly upregulated SUMOylation regulator in glioma. Moreover, SENP1 was overexpressed in gl...
Source: Experimental Cell Research - October 22, 2023 Category: Cytology Authors: Xin Liu Shenglin Zhang Yi Dong Yunpeng Xie Qingshan Li Source Type: research

Mitochondrial response of glioma cells to temozolomide
Exp Cell Res. 2023 Oct 20:113825. doi: 10.1016/j.yexcr.2023.113825. Online ahead of print.ABSTRACTMetabolic adaptations are central for carcinogenesis and response to therapy, but little is known about the contribution of mitochondrial dynamics to the response of glioma cells to the standard treatment with temozolomide (TMZ). Glioma cells responded to TMZ with mitochondrial mass increased and the production of round structures of dysfunctional mitochondria. At single-cell level, asymmetric mitosis contributed to the heterogeneity of mitochondrial levels. It affected the fitness of cells in control and treated condition, in...
Source: Experimental Cell Research - October 22, 2023 Category: Cytology Authors: Luana Su éling Lenz Daphne Torgo de Lemos Julieti Huch Buss Luiza Cherobini Pereira Mardja Bueno Eduardo Cremonese Filippi-Chiela Guido Lenz Source Type: research

SENP1-mediated SUMOylation of SIRT1 affects glioma development through the NF- κB pathway
Exp Cell Res. 2023 Oct 20:113822. doi: 10.1016/j.yexcr.2023.113822. Online ahead of print.ABSTRACTGliomas are the most common primary brain tumors in adults. Although they exist in different malignant stages, most gliomas are clinically challenging because of their infiltrative growth patterns and inherent relapse tendency with increased malignancy. Epigenetic alterations have been suggested to be an important factor for glioma genesis. Using mRNA probe hybridization, we identified SUMO-specific protease 1 (SENP1) as the most significantly upregulated SUMOylation regulator in glioma. Moreover, SENP1 was overexpressed in gl...
Source: Experimental Cell Research - October 22, 2023 Category: Cytology Authors: Xin Liu Shenglin Zhang Yi Dong Yunpeng Xie Qingshan Li Source Type: research

Mitochondrial response of glioma cells to temozolomide
Exp Cell Res. 2023 Oct 20:113825. doi: 10.1016/j.yexcr.2023.113825. Online ahead of print.ABSTRACTMetabolic adaptations are central for carcinogenesis and response to therapy, but little is known about the contribution of mitochondrial dynamics to the response of glioma cells to the standard treatment with temozolomide (TMZ). Glioma cells responded to TMZ with mitochondrial mass increased and the production of round structures of dysfunctional mitochondria. At single-cell level, asymmetric mitosis contributed to the heterogeneity of mitochondrial levels. It affected the fitness of cells in control and treated condition, in...
Source: Experimental Cell Research - October 22, 2023 Category: Cytology Authors: Luana Su éling Lenz Daphne Torgo de Lemos Julieti Huch Buss Luiza Cherobini Pereira Mardja Bueno Eduardo Cremonese Filippi-Chiela Guido Lenz Source Type: research

Microarray analysis identifies human apoA-I < sub > Milano < /sub > and apoA-II as determinants of the liver gene expression related to lipid and energy metabolism
Exp Cell Res. 2023 Oct 18;433(1):113826. doi: 10.1016/j.yexcr.2023.113826. Online ahead of print.ABSTRACTThe phenotype of individuals carrying the apolipoprotein A-IMilano (apoA-IM), the mutant form of human apoA-I (apoA-I), is characterized by very low concentrations of HDL and apoA-I, and hypertriglyceridemia. Paradoxically, these subjects are not found to be at increased risk of premature cardiovascular disease compared to controls. Besides, various in vitro and in vivo studies have demonstrated that apoA-IM possesses greater anti-atherosclerotic activity compared to apoA-I. The molecular mechanisms explaining the apoA-...
Source: Experimental Cell Research - October 20, 2023 Category: Cytology Authors: Giulia S Ganzetti Cinzia Parolini Source Type: research

"The recent development of carbon-based nanoparticles as a novel approach to skin tissue care and management - A review"
Exp Cell Res. 2023 Oct 17:113821. doi: 10.1016/j.yexcr.2023.113821. Online ahead of print.ABSTRACTSince the skin is the first barrier of the body's defense against pathogens, delays in the healing process are affected by infections. Therefore, applying advanced substitute assistance improves the patient's quality of life. Carbon-based nanomaterials show better capabilities than conventional methods for managing skin wound infections. Due to their physicochemical properties such as small size, large surface area, great surface-to-volume ratio, and excellent ability to communicate with the cells and tissue, carbon-based nano...
Source: Experimental Cell Research - October 20, 2023 Category: Cytology Authors: Negar Karimi Hajishoreh Zahra Jamalpoor Ramin Rasouli Amir Nezami Asl Roghayeh Sheervalilou Abolfazl Akbarzadeh Source Type: research