Quercitrin attenuates the progression of osteoarthritis via inhibiting NF- κB signaling pathways and enhance glucose transport capacity
In this study, we aimed to investigate the anti-inflammatory activity, anti-ECM degradation property, and glucose transport capacity of quercitrin (QCT) on IL-1β-treated rat primary chondrocytes. Rat primary chondrocytes were treated with IL-1β to simulate inflammatory environmental conditions and OA in vitro. We examined the effects of QCT at concentrations ranging from 0 to 200 μM on the viability of rat chondrocytes and selected 5 μM for further study. Using qRT-PCR, immunofluorescent, immunocytochemistry, and western blotting techniques, we identified the potential molecular mechanisms and signaling pathways that a...
Source: Experimental Cell Research - November 12, 2023 Category: Cytology Authors: Shigang Qiao Runze Zhao Shuangjian He Xuejie Fu Jianzhong An Tingting Xia Source Type: research
Quercitrin attenuates the progression of osteoarthritis via inhibiting NF- κB signaling pathways and enhance glucose transport capacity
In this study, we aimed to investigate the anti-inflammatory activity, anti-ECM degradation property, and glucose transport capacity of quercitrin (QCT) on IL-1β-treated rat primary chondrocytes. Rat primary chondrocytes were treated with IL-1β to simulate inflammatory environmental conditions and OA in vitro. We examined the effects of QCT at concentrations ranging from 0 to 200 μM on the viability of rat chondrocytes and selected 5 μM for further study. Using qRT-PCR, immunofluorescent, immunocytochemistry, and western blotting techniques, we identified the potential molecular mechanisms and signaling pathways that a...
Source: Experimental Cell Research - November 12, 2023 Category: Cytology Authors: Shigang Qiao Runze Zhao Shuangjian He Xuejie Fu Jianzhong An Tingting Xia Source Type: research
Quercitrin attenuates the progression of osteoarthritis via inhibiting NF- κB signaling pathways and enhance glucose transport capacity
In this study, we aimed to investigate the anti-inflammatory activity, anti-ECM degradation property, and glucose transport capacity of quercitrin (QCT) on IL-1β-treated rat primary chondrocytes. Rat primary chondrocytes were treated with IL-1β to simulate inflammatory environmental conditions and OA in vitro. We examined the effects of QCT at concentrations ranging from 0 to 200 μM on the viability of rat chondrocytes and selected 5 μM for further study. Using qRT-PCR, immunofluorescent, immunocytochemistry, and western blotting techniques, we identified the potential molecular mechanisms and signaling pathways that a...
Source: Experimental Cell Research - November 12, 2023 Category: Cytology Authors: Shigang Qiao Runze Zhao Shuangjian He Xuejie Fu Jianzhong An Tingting Xia Source Type: research
ADAMTS-1 has nuclear localization in cells with epithelial origin and leads to decreased cell migration
Exp Cell Res. 2023 Nov 9:113852. doi: 10.1016/j.yexcr.2023.113852. Online ahead of print.ABSTRACTIn the study of tumorigenesis, the involvement of molecules within the extracellular matrix (ECM) is crucial. ADAMTSs (A Disintegrin and Metalloproteinase with Thrombospondin motifs), a group of secreted proteases known for their role in ECM remodeling, were primarily considered to be extracellular proteases. However, our research specifically detected ADAMTS-1, a member of this family, predominantly within the nucleus of mammary cells. Our main objective was to understand the mechanism of ADAMTS-1 translocation to the nucleus ...
Source: Experimental Cell Research - November 11, 2023 Category: Cytology Authors: Su ély V Silva Ma íra A Lima Louis Hodgson Vanessa M Freitas Source Type: research
ADAMTS-1 has nuclear localization in cells with epithelial origin and leads to decreased cell migration
Exp Cell Res. 2023 Nov 9:113852. doi: 10.1016/j.yexcr.2023.113852. Online ahead of print.ABSTRACTIn the study of tumorigenesis, the involvement of molecules within the extracellular matrix (ECM) is crucial. ADAMTSs (A Disintegrin and Metalloproteinase with Thrombospondin motifs), a group of secreted proteases known for their role in ECM remodeling, were primarily considered to be extracellular proteases. However, our research specifically detected ADAMTS-1, a member of this family, predominantly within the nucleus of mammary cells. Our main objective was to understand the mechanism of ADAMTS-1 translocation to the nucleus ...
Source: Experimental Cell Research - November 11, 2023 Category: Cytology Authors: Su ély V Silva Ma íra A Lima Louis Hodgson Vanessa M Freitas Source Type: research
The anti-SARS-CoV-2 BNT162b2 vaccine suppresses mithramycin-induced erythroid differentiation and expression of embryo-fetal globin genes in human erythroleukemia K562 cells
This study thus provides information suggesting the need of great attention on possible alteration of hematopoietic parameters following SARS-CoV-2 infection and/or COVID-19 vaccination.PMID:37944576 | DOI:10.1016/j.yexcr.2023.113853 (Source: Experimental Cell Research)
Source: Experimental Cell Research - November 9, 2023 Category: Cytology Authors: Matteo Zurlo Jessica Gasparello Marco Verona Chiara Papi Lucia Carmela Cosenza Alessia Finotti Giovanni Marzaro Roberto Gambari Source Type: research
The anti-SARS-CoV-2 BNT162b2 vaccine suppresses mithramycin-induced erythroid differentiation and expression of embryo-fetal globin genes in human erythroleukemia K562 cells
This study thus provides information suggesting the need of great attention on possible alteration of hematopoietic parameters following SARS-CoV-2 infection and/or COVID-19 vaccination.PMID:37944576 | DOI:10.1016/j.yexcr.2023.113853 (Source: Experimental Cell Research)
Source: Experimental Cell Research - November 9, 2023 Category: Cytology Authors: Matteo Zurlo Jessica Gasparello Marco Verona Chiara Papi Lucia Carmela Cosenza Alessia Finotti Giovanni Marzaro Roberto Gambari Source Type: research
The anti-SARS-CoV-2 BNT162b2 vaccine suppresses mithramycin-induced erythroid differentiation and expression of embryo-fetal globin genes in human erythroleukemia K562 cells
This study thus provides information suggesting the need of great attention on possible alteration of hematopoietic parameters following SARS-CoV-2 infection and/or COVID-19 vaccination.PMID:37944576 | DOI:10.1016/j.yexcr.2023.113853 (Source: Experimental Cell Research)
Source: Experimental Cell Research - November 9, 2023 Category: Cytology Authors: Matteo Zurlo Jessica Gasparello Marco Verona Chiara Papi Lucia Carmela Cosenza Alessia Finotti Giovanni Marzaro Roberto Gambari Source Type: research
Hypoxia promotes the growth and metastasis of ovarian cancer cells by suppressing ferroptosis via upregulating SLC2A12
CONCLUSION: Hypoxia promoted ovarian cancer progression by upregulating SLC2A12 and then regulating glutathione metabolism to inhibit ferroptosis.PMID:37940066 | DOI:10.1016/j.yexcr.2023.113851 (Source: Experimental Cell Research)
Source: Experimental Cell Research - November 8, 2023 Category: Cytology Authors: Mingmei Li Li Li Xiaoxiao Cheng Longyu Li Kaijia Tu Source Type: research
Reactive oxygen species downregulate dystroglycans in the megakaryocytes of rats with arterial hypertension
In this study, we aimed to determine the expression of dystroglycans (Dg) and ENaC in platelet progenitors (megakaryocytes) and their surrounding niches. Thin sections of bone marrow from 5- and 28-week-old spontaneous hypertensive rats (SHR) were compared to age-matched normotensive rats (WKY). Cytometry and immunohistochemical assays demonstrated an oxidative environment in SHR bone marrow, characterized by high levels of myeloperoxidase and 3-nitrotyrosine and downregulation of peroxiredoxin II. In addition, transmission electron micrography and confocal microscopy revealed morphological changes in platelets and Mgks fr...
Source: Experimental Cell Research - November 6, 2023 Category: Cytology Authors: Doris Cerecedo Ivette Mart ínez-Vieyra Isaac Hern ández-Rojo Arturo Hern ández-Cruz Ruth Rinc ón-Heredia Diana Mill án-Aldaco Maria Eugenia Mendoza-Garrido Source Type: research
Reactive oxygen species downregulate dystroglycans in the megakaryocytes of rats with arterial hypertension
In this study, we aimed to determine the expression of dystroglycans (Dg) and ENaC in platelet progenitors (megakaryocytes) and their surrounding niches. Thin sections of bone marrow from 5- and 28-week-old spontaneous hypertensive rats (SHR) were compared to age-matched normotensive rats (WKY). Cytometry and immunohistochemical assays demonstrated an oxidative environment in SHR bone marrow, characterized by high levels of myeloperoxidase and 3-nitrotyrosine and downregulation of peroxiredoxin II. In addition, transmission electron micrography and confocal microscopy revealed morphological changes in platelets and Mgks fr...
Source: Experimental Cell Research - November 6, 2023 Category: Cytology Authors: Doris Cerecedo Ivette Mart ínez-Vieyra Isaac Hern ández-Rojo Arturo Hern ández-Cruz Ruth Rinc ón-Heredia Diana Mill án-Aldaco Maria Eugenia Mendoza-Garrido Source Type: research
Reactive oxygen species downregulate dystroglycans in the megakaryocytes of rats with arterial hypertension
In this study, we aimed to determine the expression of dystroglycans (Dg) and ENaC in platelet progenitors (megakaryocytes) and their surrounding niches. Thin sections of bone marrow from 5- and 28-week-old spontaneous hypertensive rats (SHR) were compared to age-matched normotensive rats (WKY). Cytometry and immunohistochemical assays demonstrated an oxidative environment in SHR bone marrow, characterized by high levels of myeloperoxidase and 3-nitrotyrosine and downregulation of peroxiredoxin II. In addition, transmission electron micrography and confocal microscopy revealed morphological changes in platelets and Mgks fr...
Source: Experimental Cell Research - November 6, 2023 Category: Cytology Authors: Doris Cerecedo Ivette Mart ínez-Vieyra Isaac Hern ández-Rojo Arturo Hern ández-Cruz Ruth Rinc ón-Heredia Diana Mill án-Aldaco Maria Eugenia Mendoza-Garrido Source Type: research
Calcineurin suppresses rat H9c2 cardiomyocyteprotective autophagy under chronic intermittent hypoxia by downregulating the AMPK pathway
Exp Cell Res. 2023 Nov 3:113850. doi: 10.1016/j.yexcr.2023.113850. Online ahead of print.ABSTRACTCalcineurin plays a key role in cardiovascular pathogenesis by exerting pro-apoptotic effects in cardiomyocytes. However, whether calcineurin can regulate cardiomyocyte autophagy under conditions of chronic intermittent hypoxia (CIH) remains unclear. Here, we showed that CIH induced calcineurin activity in H9c2 cells, which attenuated adenosine monophosphate-activated protein kinase (AMPK) signaling and inhibited autophagy. In H9c2 cells, autophagy levels, LC3 expression, and AMPK phosphorylation were significantly elevated und...
Source: Experimental Cell Research - November 5, 2023 Category: Cytology Authors: Changjiang Ke Yongjun Huang Zhenyu Mao Zhenghua Ke Zeng Wang Ruyou Li Shenghua Long Yuping Guo Fei Wang Meng Qian Ruxia Zhao Juan Zheng Sheng Xie Source Type: research
Knockdown of Chronophage in the nervous system mimics features of neurodevelopmental disorders caused by BCL11 A/B variants
Exp Cell Res. 2023 Nov 3:113827. doi: 10.1016/j.yexcr.2023.113827. Online ahead of print.ABSTRACTNeurodevelopmental disorders (NDD) are a group of disorders that include intellectual disability. Although several genes have been implicated in NDD, the molecular mechanisms underlying its pathogenesis remain unclear. Therefore, it is important to develop novel models to analyze the functions of NDD-causing genes in vivo. Recently, rare pathogenic variants of the B-cell lymphoma/leukemia 11A/B (BCL11 A/B) gene have been identified in several patients with NDD. Drosophila carries the Chronophage (Cph) gene, which has been predi...
Source: Experimental Cell Research - November 5, 2023 Category: Cytology Authors: Mizuki Yamaguchi Man Anh Huynh Tomohiro Chiyonobu Hideki Yoshida Source Type: research
Involvement of estrogen receptor activation in kaempferol-3-O-glucoside's protection against aging-related cognition impairment and microglial inflammation
This study aimed to determine whether K-3-G improved cognition during the aging process, with an emphasis on its effect on microglial inflammation. In vivo, K-3-G (5 or 10 mg/kg/day) was orally given to the senescence-accelerated mouse prone 8 (SAMP8) mice from six to eight-month old. In addition to mitigating the memory and learning deficits of SAMP8 mice, K-3-G upregulated the expression of ERα and ERβ in their hippocampal CA1 region, with the higher dose being more effective. Less Iba-1+ microglial cells presented in SAMP8 mice treated with K-3-G. The formation of NLR Family Pyrin Domain Containing 3 (NLRP3) complex, ...
Source: Experimental Cell Research - November 5, 2023 Category: Cytology Authors: Hong Liu Yang Huang Jing Yang Xuejiao Xu Qiaomei Dai Yuwei Zhang Li Zhao Mengdi Zhang Jing Zhang Tonghui Liu Lili Zhong Source Type: research
