IRX2 regulates endometrial carcinoma oncogenesis by transcriptional repressing RUVBL1
In conclusion, our findings indicate that decreased expression of IRX2 facilitates EC cell growth through the regulation of RUVBL1 expression, thereby contributing to the development of EC. Hence, targeting the IRX2-RUVBL1 axis holds promise as a potential therapeutic strategy for EC treatment.PMID:38042247 | DOI:10.1016/j.yexcr.2023.113866 (Source: Experimental Cell Research)
Source: Experimental Cell Research - December 2, 2023 Category: Cytology Authors: Qinyang Xu Wanzhen Zhou Yuedi Zhou Xueying Zhang Rongzhen Jiang Zhihong Ai Jing Chen Li Ma Source Type: research
Deubiquitinating enzyme OTUD4 regulates metastasis in triple-negative breast cancer by stabilizing Snail1
Exp Cell Res. 2023 Nov 29:113864. doi: 10.1016/j.yexcr.2023.113864. Online ahead of print.ABSTRACTMetastasis is the primary cause of cancer-related deaths and remains poorly understood. Deubiquitinase OTU domain containing 4 (OTUD4) has been reported to regulate antiviral immune responses and resistance to radio- or chemo-therapies in certain cancers. However, the role of OTUD4 in cancer metastasis remain unknown. Here, we demonstrate that the depletion of OTUD4 in triple-negative breast cancer (TNBC) cells markedly suppress cell clonogenic ability, migration, invasion and CSC population in vitro as well as metastasis in v...
Source: Experimental Cell Research - December 1, 2023 Category: Cytology Authors: Xiuqing Ma Rui Wan Yalei Wen Tongzheng Liu Yan Song Yingjie Zhu Source Type: research
Deubiquitinating enzyme OTUD4 regulates metastasis in triple-negative breast cancer by stabilizing Snail1
Exp Cell Res. 2023 Nov 29:113864. doi: 10.1016/j.yexcr.2023.113864. Online ahead of print.ABSTRACTMetastasis is the primary cause of cancer-related deaths and remains poorly understood. Deubiquitinase OTU domain containing 4 (OTUD4) has been reported to regulate antiviral immune responses and resistance to radio- or chemo-therapies in certain cancers. However, the role of OTUD4 in cancer metastasis remain unknown. Here, we demonstrate that the depletion of OTUD4 in triple-negative breast cancer (TNBC) cells markedly suppress cell clonogenic ability, migration, invasion and CSC population in vitro as well as metastasis in v...
Source: Experimental Cell Research - December 1, 2023 Category: Cytology Authors: Xiuqing Ma Rui Wan Yalei Wen Tongzheng Liu Yan Song Yingjie Zhu Source Type: research
Insulin promotes growth in breast cancer cells through the type I IGF receptor in insulin receptor deficient cells
Exp Cell Res. 2023 Nov 28:113862. doi: 10.1016/j.yexcr.2023.113862. Online ahead of print.ABSTRACTBreast cancer is the most common cancer in women. The upregulation of insulin-like growth factor (IGF) system observed in certain types of breast cancers was linked to growth, metastasis, and survival resulting in multiple strategies designed to target the type I IGF receptor (IGF-1R) in breast cancer. These attempts failed to prove beneficial and it has been suggested that insulin receptor (IR) could also play an important role in breast cancer biology. To better understand the IR's role in breast cancer cells, the receptor w...
Source: Experimental Cell Research - November 30, 2023 Category: Cytology Authors: Marvis Monteiro Xihong Zhang Douglas Yee Source Type: research
Insulin promotes growth in breast cancer cells through the type I IGF receptor in insulin receptor deficient cells
Exp Cell Res. 2023 Nov 28:113862. doi: 10.1016/j.yexcr.2023.113862. Online ahead of print.ABSTRACTBreast cancer is the most common cancer in women. The upregulation of insulin-like growth factor (IGF) system observed in certain types of breast cancers was linked to growth, metastasis, and survival resulting in multiple strategies designed to target the type I IGF receptor (IGF-1R) in breast cancer. These attempts failed to prove beneficial and it has been suggested that insulin receptor (IR) could also play an important role in breast cancer biology. To better understand the IR's role in breast cancer cells, the receptor w...
Source: Experimental Cell Research - November 30, 2023 Category: Cytology Authors: Marvis Monteiro Xihong Zhang Douglas Yee Source Type: research
Cell death classification: A new insight based on molecular mechanisms
Exp Cell Res. 2023 Nov 25:113860. doi: 10.1016/j.yexcr.2023.113860. Online ahead of print.ABSTRACTCells tend to disintegrate themselves or are forced to undergo such destructive processes in critical circumstances. This complex cellular function necessitates various mechanisms and molecular pathways in order to be executed. The very nature of cell death is essentially important and vital for maintaining homeostasis, thus any type of disturbing occurrence might lead to different sorts of diseases and dysfunctions. Cell death has various modalities and yet, every now and then, a new type of this elegant procedure gets to be ...
Source: Experimental Cell Research - November 28, 2023 Category: Cytology Authors: Sepehr Dehghan Nasim Kheshtchin Shaghayegh Hassannezhad Maryam Soleimani Source Type: research
Cell death classification: A new insight based on molecular mechanisms
Exp Cell Res. 2023 Nov 25:113860. doi: 10.1016/j.yexcr.2023.113860. Online ahead of print.ABSTRACTCells tend to disintegrate themselves or are forced to undergo such destructive processes in critical circumstances. This complex cellular function necessitates various mechanisms and molecular pathways in order to be executed. The very nature of cell death is essentially important and vital for maintaining homeostasis, thus any type of disturbing occurrence might lead to different sorts of diseases and dysfunctions. Cell death has various modalities and yet, every now and then, a new type of this elegant procedure gets to be ...
Source: Experimental Cell Research - November 28, 2023 Category: Cytology Authors: Sepehr Dehghan Nasim Kheshtchin Shaghayegh Hassannezhad Maryam Soleimani Source Type: research
Cell death classification: A new insight based on molecular mechanisms
Exp Cell Res. 2023 Nov 25:113860. doi: 10.1016/j.yexcr.2023.113860. Online ahead of print.ABSTRACTCells tend to disintegrate themselves or are forced to undergo such destructive processes in critical circumstances. This complex cellular function necessitates various mechanisms and molecular pathways in order to be executed. The very nature of cell death is essentially important and vital for maintaining homeostasis, thus any type of disturbing occurrence might lead to different sorts of diseases and dysfunctions. Cell death has various modalities and yet, every now and then, a new type of this elegant procedure gets to be ...
Source: Experimental Cell Research - November 28, 2023 Category: Cytology Authors: Sepehr Dehghan Nasim Kheshtchin Shaghayegh Hassannezhad Maryam Soleimani Source Type: research
LRP5 competes for SPOP binding to enhance tumorigenesis mediated by Daxx and PD-L1 in prostate cancer
Exp Cell Res. 2023 Nov 24:113857. doi: 10.1016/j.yexcr.2023.113857. Online ahead of print.ABSTRACTGenetic factors coordinate with environmental factors to drive the pathogenesis of prostate adenocarcinoma (PRAD). SPOP is one of the most mutated genes and LRP5 mediates lipid metabolism that is abnormally altered in PRAD. Here, we investigated the potential cross-talk between SPOP and LRP5 in PRAD. We find a negative correlation between SPOP and LRP5 proteins in PRAD. SPOP knockdown increased LRP5 protein while SPOP overexpression resulted in LRP5 reduction that was fully rescued by proteasome inhibitors. LRP5 intracellular ...
Source: Experimental Cell Research - November 26, 2023 Category: Cytology Authors: Sishun Gan Fajun Qu Xiangmin Zhang Xiuwu Pan Da Xu Xingang Cui Jianquan Hou Source Type: research
LRP5 competes for SPOP binding to enhance tumorigenesis mediated by Daxx and PD-L1 in prostate cancer
Exp Cell Res. 2023 Nov 24:113857. doi: 10.1016/j.yexcr.2023.113857. Online ahead of print.ABSTRACTGenetic factors coordinate with environmental factors to drive the pathogenesis of prostate adenocarcinoma (PRAD). SPOP is one of the most mutated genes and LRP5 mediates lipid metabolism that is abnormally altered in PRAD. Here, we investigated the potential cross-talk between SPOP and LRP5 in PRAD. We find a negative correlation between SPOP and LRP5 proteins in PRAD. SPOP knockdown increased LRP5 protein while SPOP overexpression resulted in LRP5 reduction that was fully rescued by proteasome inhibitors. LRP5 intracellular ...
Source: Experimental Cell Research - November 26, 2023 Category: Cytology Authors: Sishun Gan Fajun Qu Xiangmin Zhang Xiuwu Pan Da Xu Xingang Cui Jianquan Hou Source Type: research
Cardiac ischemic preconditioning promotes cMyBP-C phosphorylation by inhibiting the calpain-mediated proteolysis
In this study, IPC was found to increase the phosphorylation level of cMyBP-C, companying with the decreased calpain activity in the collected perfusate samples. Mechanistically, we confirmed that IPC promoted cMyBP-C phosphorylation and inhibited calpain-mediated cMyBP-C proteolysis. Moreover, inhibition of calpain activity significantly increased the phosphorylated cMyBP-C level by using calpain inhibitor (MG-101), and subsequently promoted stabilization and secretion of cMyBP-C. Functionally, adeno-associated virus (AAV)-mediated overexpression of mutated phosphorylation motif site of cMyBP-C exhibited impaired IPC-medi...
Source: Experimental Cell Research - November 24, 2023 Category: Cytology Authors: Cheng Liang Zulibiya Aisa Longfei Sun Baojian Zhang Source Type: research
Circular RNA DLGAP4 alleviates sevoflurane-induced neurotoxicity by regulating miR-9-5p/Sirt1/BDNF pathway
CONCLUSIONS: Our studies found that circDLGAP4 relieved sevoflurane-induced neurotoxicity by sponging miR-9-5p to regulate Sirt1/BDNF pathway.PMID:38000773 | DOI:10.1016/j.yexcr.2023.113861 (Source: Experimental Cell Research)
Source: Experimental Cell Research - November 24, 2023 Category: Cytology Authors: Dongying Zhang Liuping Wu Long Ma Jiazheng Wang Linjie Niu Ping He Source Type: research
Cardiac ischemic preconditioning promotes cMyBP-C phosphorylation by inhibiting the calpain-mediated proteolysis
In this study, IPC was found to increase the phosphorylation level of cMyBP-C, companying with the decreased calpain activity in the collected perfusate samples. Mechanistically, we confirmed that IPC promoted cMyBP-C phosphorylation and inhibited calpain-mediated cMyBP-C proteolysis. Moreover, inhibition of calpain activity significantly increased the phosphorylated cMyBP-C level by using calpain inhibitor (MG-101), and subsequently promoted stabilization and secretion of cMyBP-C. Functionally, adeno-associated virus (AAV)-mediated overexpression of mutated phosphorylation motif site of cMyBP-C exhibited impaired IPC-medi...
Source: Experimental Cell Research - November 24, 2023 Category: Cytology Authors: Cheng Liang Zulibiya Aisa Longfei Sun Baojian Zhang Source Type: research
Circular RNA DLGAP4 alleviates sevoflurane-induced neurotoxicity by regulating miR-9-5p/Sirt1/BDNF pathway
CONCLUSIONS: Our studies found that circDLGAP4 relieved sevoflurane-induced neurotoxicity by sponging miR-9-5p to regulate Sirt1/BDNF pathway.PMID:38000773 | DOI:10.1016/j.yexcr.2023.113861 (Source: Experimental Cell Research)
Source: Experimental Cell Research - November 24, 2023 Category: Cytology Authors: Dongying Zhang Liuping Wu Long Ma Jiazheng Wang Linjie Niu Ping He Source Type: research
Ca < sup > 2+ < /sup > -activated K < sup > + < /sup > channels modulate membrane potential in the human parathyroid cell: Possible role in exocytosis
Exp Cell Res. 2023 Nov 21:113858. doi: 10.1016/j.yexcr.2023.113858. Online ahead of print.ABSTRACTThe relationships between parathyroid hormone (PTH) secretion and parathyroid cell membrane potential, including the identities and roles of K+ channels that regulate and/or modulate membrane potential are not well defined. Here we have used Western blot/immunohistochemistry as well as patch-clamp and perifusion techniques to identify and localize specific K+ channels in parathyroid cells and to investigate their roles in the control of membrane potential and PTH secretion. We also re-investigated the relationship between memb...
Source: Experimental Cell Research - November 23, 2023 Category: Cytology Authors: Robert Br änström Vladana Vukojevi ć Ming Lu Ivan Shabo Hee-Chang Mun Arthur D Conigrave Lars-Ove Farnebo Catharina Larsson Source Type: research
