Silencing hsa_circ_0032449 inhibits the pancreatic differentiation of human embryonic stem cells via the hsa_miR-195-5p/CCND1/PI3K/AKT signaling pathway
Exp Cell Res. 2023 Dec 8:113879. doi: 10.1016/j.yexcr.2023.113879. Online ahead of print.ABSTRACTStem cell-derived β cells (SC-β cells) differentiated from stem cell-derived pancreatic progenitor (PP) cells are promising tools for enabling normal glucose control of islet transplants and have therapeutic potential for type 1 diabetes treatment. Pancreatic specification is essential for SC-β cell induction in vitro and low-quality PP cells may convert into derivatives of non-pancreatic lineages both in vivo and in vitro, impeding PP-derived β cell safety and differentiation efficiency. Circular RNA (circRNA) commonly det...
Source: Experimental Cell Research - December 10, 2023 Category: Cytology Authors: Yang Xu Tianxin Xu Yan Huang Jian Wan Zhaoyan Jiang Source Type: research
Non-adherent culture method affects the proliferation and apoptosis of mesenchymal stem cells through inhibiting LINC00707 to promote RNF6-mediated QKI ubiquitination
In this study, sequencing analysis revealed that LINC00707 was significantly decreased in non-adherent human MSCs (non-AC-hMSCs) compared to adherent human MSCs. Moreover, LINC00707 overexpression promoted non-AChMSC proliferation, cell cycle progression from the G0/G1 phase to the S phase and inhibited apoptosis, whereas LINC00707 silencing had the opposite effect. Furthermore, LINC00707 interacted directly with the quaking (QKI) protein and enhanced the E3 ubiquitin-protein ligase ring finger protein 6 (RNF6)-mediated ubiquitination of the QKI protein. Additionally, the overexpression of QKI rescued the promotive effects...
Source: Experimental Cell Research - December 10, 2023 Category: Cytology Authors: Baoping Deng Weiping Deng Hongmei Zheng Yue Wei Jinfeng Zhang Na Zeng Yulan He Runmin Guo Source Type: research
Sumoylation and phosphorylation of PTEN boosts and curtails autophagy respectively by influencing cell membrane localisation
Exp Cell Res. 2023 Dec 8:113872. doi: 10.1016/j.yexcr.2023.113872. Online ahead of print.ABSTRACTAutophagy is involved in the entirety of cellular survival, homeostasis and death which becomes more self-evident when its dysregulation is implicated in several pathological conditions. PTEN positively regulates autophagy and like other proteins undergo post-translational modifications. It is crucial to investigate the relationship between PTEN and autophagy as it is generally observed to be negligible in PTEN deficient cancer cells. Here, we have shown that such modifications of PTEN namely sumoylation and phosphorylation upr...
Source: Experimental Cell Research - December 10, 2023 Category: Cytology Authors: Debojyoti De Ginia Ghosh Parimal Karmakar Source Type: research
Silencing hsa_circ_0032449 inhibits the pancreatic differentiation of human embryonic stem cells via the hsa_miR-195-5p/CCND1/PI3K/AKT signaling pathway
Exp Cell Res. 2023 Dec 8:113879. doi: 10.1016/j.yexcr.2023.113879. Online ahead of print.ABSTRACTStem cell-derived β cells (SC-β cells) differentiated from stem cell-derived pancreatic progenitor (PP) cells are promising tools for enabling normal glucose control of islet transplants and have therapeutic potential for type 1 diabetes treatment. Pancreatic specification is essential for SC-β cell induction in vitro and low-quality PP cells may convert into derivatives of non-pancreatic lineages both in vivo and in vitro, impeding PP-derived β cell safety and differentiation efficiency. Circular RNA (circRNA) commonly det...
Source: Experimental Cell Research - December 10, 2023 Category: Cytology Authors: Yang Xu Tianxin Xu Yan Huang Jian Wan Zhaoyan Jiang Source Type: research
Advances in bi-directional relationships for EZH2 and oxidative stress
Exp Cell Res. 2023 Dec 7:113876. doi: 10.1016/j.yexcr.2023.113876. Online ahead of print.ABSTRACTOver the past two decades, polycomb repressive complex 2(PRC2) has emerged as a vital repressive complex in overall cell fate determination. In mammals, enhancer of zeste homolog 2 (EHZ2), which is the core component of PRC2, has also been recognized as an important regulator of inflammatory, redox, tumorigenesis and damage repair signalling networks. To exert these effects, EZH2 must regulate target genes epigenetically or interact directly with other gene expression-regulating factors, such as LncRNAs and microRNAs. Our revie...
Source: Experimental Cell Research - December 9, 2023 Category: Cytology Authors: Heng Qin Chang Liu Changqing Li Chencheng Feng None Bo Huang Source Type: research
The inhibition of VDAC1 oligomerization promotes pigmentation through the CaMK-CRTCs/CREB-MITF pathway
In this study, we found that VDAC1 oligomerization inhibitors, VBIT-12 and NSC-15364, promote melanogenesis, dendrite formation and melanosome transport in human epidermal melanocytes (HEMCs). Mechanistically, treatment of HEMCs with an oligomerization inhibitor increased the level of cytoplasmic calcium ions, which activated calcium-calmodulin dependent protein kinase (CaMK) and led to the phosphorylation of CREB and the nuclear translocation of CREB-regulated transcription coactivators (CRTCs). Subsequently, CRTCs, p-CREB and CREB-binding protein (CBP) in the nucleus cooperatively recruit the transcription machinery to i...
Source: Experimental Cell Research - December 9, 2023 Category: Cytology Authors: Jinpeng Lv Ximei Zhang Xiaohong An Yan Cao Duo Meng Kun Zou Rongyin Gao Ruzhi Zhang Source Type: research
Salinomycin and IR780-loaded upconversion nanoparticles influence biological behavior of liver cancer stem cells by persistently activating the MAPK signaling pathway
Exp Cell Res. 2023 Dec 3;434(1):113865. doi: 10.1016/j.yexcr.2023.113865. Online ahead of print.ABSTRACTThe combination of chemotherapy and phototherapy has emerged as a promising therapeutic approach for enhancing the efficacy of cancer treatment and mitigating drug resistance. Salinomycin (SAL), a polyether antibiotic, exhibits potent cytotoxicity against chemotherapy-resistant cancer cells. IR780 iodide, a novel photosensitive reagent with excellent near-infrared (NIR) light absorption and photothermal conversion abilities, is suitable for use in photothermal therapy for cancers. However, both SAL and IR780 exhibit hydr...
Source: Experimental Cell Research - December 5, 2023 Category: Cytology Authors: Bingyu Zhang Zhongjie Ding Xianxin Wen Guanbin Song Qing Luo Source Type: research
Salinomycin and IR780-loaded upconversion nanoparticles influence biological behavior of liver cancer stem cells by persistently activating the MAPK signaling pathway
Exp Cell Res. 2023 Dec 3;434(1):113865. doi: 10.1016/j.yexcr.2023.113865. Online ahead of print.ABSTRACTThe combination of chemotherapy and phototherapy has emerged as a promising therapeutic approach for enhancing the efficacy of cancer treatment and mitigating drug resistance. Salinomycin (SAL), a polyether antibiotic, exhibits potent cytotoxicity against chemotherapy-resistant cancer cells. IR780 iodide, a novel photosensitive reagent with excellent near-infrared (NIR) light absorption and photothermal conversion abilities, is suitable for use in photothermal therapy for cancers. However, both SAL and IR780 exhibit hydr...
Source: Experimental Cell Research - December 5, 2023 Category: Cytology Authors: Bingyu Zhang Zhongjie Ding Xianxin Wen Guanbin Song Qing Luo Source Type: research
Salinomycin and IR780-loaded upconversion nanoparticles influence biological behavior of liver cancer stem cells by persistently activating the MAPK signaling pathway
Exp Cell Res. 2023 Dec 3:113865. doi: 10.1016/j.yexcr.2023.113865. Online ahead of print.ABSTRACTThe combination of chemotherapy and phototherapy has emerged as a promising therapeutic approach for enhancing the efficacy of cancer treatment and mitigating drug resistance. Salinomycin (SAL), a polyether antibiotic, exhibits potent cytotoxicity against chemotherapy-resistant cancer cells. IR780 iodide, a novel photosensitive reagent with excellent near-infrared (NIR) light absorption and photothermal conversion abilities, is suitable for use in photothermal therapy for cancers. However, both SAL and IR780 exhibit hydrophobic...
Source: Experimental Cell Research - December 5, 2023 Category: Cytology Authors: Bingyu Zhang Zhongjie Ding Xianxin Wen Guanbin Song Qing Luo Source Type: research
Salinomycin and IR780-loaded upconversion nanoparticles influence biological behavior of liver cancer stem cells by persistently activating the MAPK signaling pathway
Exp Cell Res. 2023 Dec 3:113865. doi: 10.1016/j.yexcr.2023.113865. Online ahead of print.ABSTRACTThe combination of chemotherapy and phototherapy has emerged as a promising therapeutic approach for enhancing the efficacy of cancer treatment and mitigating drug resistance. Salinomycin (SAL), a polyether antibiotic, exhibits potent cytotoxicity against chemotherapy-resistant cancer cells. IR780 iodide, a novel photosensitive reagent with excellent near-infrared (NIR) light absorption and photothermal conversion abilities, is suitable for use in photothermal therapy for cancers. However, both SAL and IR780 exhibit hydrophobic...
Source: Experimental Cell Research - December 5, 2023 Category: Cytology Authors: Bingyu Zhang Zhongjie Ding Xianxin Wen Guanbin Song Qing Luo Source Type: research
Upregulated T β4 expression in inflammatory bowel disease impairs the intestinal mucus barrier by inhibiting autophagy in mice
Exp Cell Res. 2023 Dec 2:113871. doi: 10.1016/j.yexcr.2023.113871. Online ahead of print.ABSTRACTDisrupted intestinal barrier homeostasis is fundamental to inflammatory bowel disease. Thymosin β4 (Tβ4) improves inflammation and has beneficial effects in dry-eye diseases, but its effects on the intestinal mucus barrier remain unknown. Therefore, this study evaluated the underlying regulatory mechanisms and effects of Tβ4 by examining Tβ4 expression in a mouse model with dextran sodium sulfate (DSS)-induced colitis and colonic barrier damage. Additionally, we intraperitoneally injected C57BL/6 mice with Tβ4 to assess ba...
Source: Experimental Cell Research - December 4, 2023 Category: Cytology Authors: Menghao Hao Ke Zhong Xiaoqin Bai Shiyan Wu Lu Li Yumei He Zhiming Wang Xiaobin Sun Qiong Wang Yuanbiao Guo Yueshan Sun Liping Wu Source Type: research
Dual-specificity phosphatase 5-mediated fatty acid oxidation promotes Mycobacterium bovis BCG -induced inflammatory responses
Exp Cell Res. 2023 Dec 2:113869. doi: 10.1016/j.yexcr.2023.113869. Online ahead of print.ABSTRACTMycobacterium tuberculosis (Mtb) reprograms FAs metabolism of macrophages during infection and affects inflammatory reaction eventually, however, the mechanism remains poorly understood. Here we show that Mycobacterium bovis (BCG) induces DUSP5 expression through TLR2-MAPKs signaling pathway and promotes fatty acid oxidation (FAO). Silencing DUSP5 by adeno-associated virus vector (AAV) ameliorates lung injury and DUSP5 knockdown reduces the expression of IL-1β, IL-6 and inactivated NF-κB signaling in BCG-infected macrophages....
Source: Experimental Cell Research - December 4, 2023 Category: Cytology Authors: Jia Luo Zengjian Tian Fuyang Song Chao Ren Wenmiao Liu Source Type: research
miR-130b regulates B cell proliferation via CYLD-mediated NF- κB signaling
In conclusion, we propose that miR-130b promotes B cell proliferation via Cyld-mediated NF-κB signaling, which provides a new theoretical basis for the molecular regulation of B cell activation.PMID:38049082 | DOI:10.1016/j.yexcr.2023.113870 (Source: Experimental Cell Research)
Source: Experimental Cell Research - December 4, 2023 Category: Cytology Authors: Mengyun Wu Jing Zhao Wenyan Wu Chuangli Hao Yi Yang Jinping Zhang Source Type: research
Upregulated T β4 expression in inflammatory bowel disease impairs the intestinal mucus barrier by inhibiting autophagy in mice
Exp Cell Res. 2023 Dec 2:113871. doi: 10.1016/j.yexcr.2023.113871. Online ahead of print.ABSTRACTDisrupted intestinal barrier homeostasis is fundamental to inflammatory bowel disease. Thymosin β4 (Tβ4) improves inflammation and has beneficial effects in dry-eye diseases, but its effects on the intestinal mucus barrier remain unknown. Therefore, this study evaluated the underlying regulatory mechanisms and effects of Tβ4 by examining Tβ4 expression in a mouse model with dextran sodium sulfate (DSS)-induced colitis and colonic barrier damage. Additionally, we intraperitoneally injected C57BL/6 mice with Tβ4 to assess ba...
Source: Experimental Cell Research - December 4, 2023 Category: Cytology Authors: Menghao Hao Ke Zhong Xiaoqin Bai Shiyan Wu Lu Li Yumei He Zhiming Wang Xiaobin Sun Qiong Wang Yuanbiao Guo Yueshan Sun Liping Wu Source Type: research
Dual-specificity phosphatase 5-mediated fatty acid oxidation promotes Mycobacterium bovis BCG -induced inflammatory responses
Exp Cell Res. 2023 Dec 2:113869. doi: 10.1016/j.yexcr.2023.113869. Online ahead of print.ABSTRACTMycobacterium tuberculosis (Mtb) reprograms FAs metabolism of macrophages during infection and affects inflammatory reaction eventually, however, the mechanism remains poorly understood. Here we show that Mycobacterium bovis (BCG) induces DUSP5 expression through TLR2-MAPKs signaling pathway and promotes fatty acid oxidation (FAO). Silencing DUSP5 by adeno-associated virus vector (AAV) ameliorates lung injury and DUSP5 knockdown reduces the expression of IL-1β, IL-6 and inactivated NF-κB signaling in BCG-infected macrophages....
Source: Experimental Cell Research - December 4, 2023 Category: Cytology Authors: Jia Luo Zengjian Tian Fuyang Song Chao Ren Wenmiao Liu Source Type: research
