Effect of sodium butyrate treatment at the basolateral membranes on the tight junction barrier function via a monocarboxylate transporter in goat mammary epithelial cells
This study aimed to investigate the effect of sodium acetate and sodium butyrate (SB) stimulation direction on the TJ barrier function, which is measured with regard to transepithelial electrical resistance and fluorescein flux, in goat mammary epithelial cells. The expression and localization of the TJ proteins claudin-3 and claudin-4 were examined using Western blotting and immunofluorescence. SB treatment in the lower chamber of cell culture inserts adversely affected the TJ barrier function, whereas sodium acetate barely had any effect, regardless of stimulation direction. In addition, SB treatment in the lower chamber...
Source: Experimental Cell Research - January 31, 2024 Category: Cytology Authors: Yusaku Tsugami Naoki Suzuki Takahiro Nii Naoki Isobe Source Type: research
Pterostilbene upregulates MICA/B via the PI3K/AKT signaling pathway to enhance the capability of natural killer cells to kill cervical cancer cells
Exp Cell Res. 2024 Feb 15;435(2):113933. doi: 10.1016/j.yexcr.2024.113933. Epub 2024 Feb 1.ABSTRACTNatural killer (NK) cells are triggered by the innate immune response in the tumor microenvironment. The extensive set of stimulating and inhibiting receptors mediates the target recognition of NK cells, and controls the strength of the effector reaction countering specific targeted cells. Yet, lacking major MHC (histocompatibility complex) MICA/B class I chain-related proteins on the membrane of tumor cells results in the failure of NK cell recognition and ability to resist NK cell destruction. Searching databases and molecu...
Source: Experimental Cell Research - January 31, 2024 Category: Cytology Authors: Zuoping Li Jiaru Zhang Shiwan You Jing Zhang Yuling Zhang Zubair Akram Shiguo Sun Source Type: research
Lysine demethylase KDM3A alleviates hyperoxia-induced bronchopulmonary dysplasia in mice by promoting ETS1 expression
This study aims to investigate the effect and mechanism of lysine demethylase 3A (KDM3A) on hyperoxia-induced BPD. Hyperoxia-induced BPD mouse and alveolar epithelial cell models were constructed. The effects of hyperoxia on lung development were evaluated by histological and morphological analysis. The levels of KDM3A, E26 transformation specific-1 (ETS1), H3 lysine 9 dimethylation (H3K9me2), and endoplasmic reticulum (ER) stress-related indexes were quantified by RT-qPCR, Western blot, and IF staining. Cell apoptosis was assessed by flow cytometry and TUNEL staining. Transfection of oe-ETS1, oe-KDM3A, and sh-ETS1 was app...
Source: Experimental Cell Research - January 29, 2024 Category: Cytology Authors: Min Yang Yanping Chen Xueshan Huang Fang Shen Yanni Meng Source Type: research
The m6A methyltransferase RBM15 affects tumor cell stemness and progression of cervical cancer by regulating the stability of lncRNA HEIH
Exp Cell Res. 2024 Jan 25:113924. doi: 10.1016/j.yexcr.2024.113924. Online ahead of print.ABSTRACTCervical cancer (CC), as a common female malignant tumor in the world, is an important risk factor endangering women's health worldwide. The purpose of this study was to investigate the role of RBM15 in CC. The TCGA database was used to screen differentially expressed m6A genes in normal and tumor tissues. QRT-PCR was used to quantify HEIH, miR-802, EGFR, cell stemness, and epithelial-mesenchymal transition (EMT)-related genes. The interaction between HEIH and miR-802 was verified by dual-luciferase reporter assay and RIP assa...
Source: Experimental Cell Research - January 27, 2024 Category: Cytology Authors: Yi Quan Muchuan Zhou Jinhong Li Yihong Yang Junliang Guo Tian Tang Ping Liu Source Type: research
Thyroid cancer cell metabolism: A glance into cell culture system-based metabolomics approaches
Exp Cell Res. 2024 Feb 15;435(2):113936. doi: 10.1016/j.yexcr.2024.113936. Epub 2024 Jan 24.ABSTRACTThyroid cancer is the most common malignancy of the endocrine system and the seventh most prevalent cancer in women worldwide. It is a complex and diverse disease characterized by heterogeneity, underscoring the importance of understanding the underlying metabolic alterations within tumor cells. Metabolomics technologies offer a powerful toolset to explore and identify endogenous and exogenous biochemical reaction products, providing crucial insights into the intricate metabolic pathways and processes within living cells. Me...
Source: Experimental Cell Research - January 26, 2024 Category: Cytology Authors: Raziyeh Abooshahab Fatemeh Razavi Fatemeh Ghorbani Koroush Hooshmand Maryam Zarkesh Mehdi Hedayati Source Type: research
The insc-GAL4 driver marks distinct cell types in Drosophila midgut
This study reveals for the first time that insc-GAL4 is specific to larval AMPs and adult ISCs of the midgut.PMID:38278285 | DOI:10.1016/j.yexcr.2024.113953 (Source: Experimental Cell Research)
Source: Experimental Cell Research - January 26, 2024 Category: Cytology Authors: Akanksha Pandey Jagat Kumar Roy Source Type: research
Correction of I < sub > to < /sub > in human induced pluripotent stem Cell-derived cardiomyocyte carrying DPP6 mutation in early repolarization syndrome by CRISPR/Cas9 genome editing
The objective of the present study was to examine the electrophysiological mechanism of ERS utilizing induced pluripotent stem cells (iPSCs) and CRISPR/Cas9 genome editing. Whole genome sequencing was used to identify the DPP6 (c.2561T > C/p.L854P) variant in four families with sudden cardiac arrest induced by ERS. Cardiomyocytes were generated from iPSCs from a 14-year-old boy in the four families with ERS and an unrelated healthy control subject. Patch clamp recordings revealed more significant prolongation of the action potential duration (APD) and increased transient outward potassium current (Ito) (103.97 ± 18.73 ...
Source: Experimental Cell Research - January 25, 2024 Category: Cytology Authors: Yang Wu Xiang-Jun Kong Ying-Ying Ji Jun Fan Xu-Miao Chen Cheng-Cheng Ji Yun-Jiu Cheng Su-Hua Wu Source Type: research
HECW1 restrains cervical cancer cell growth by promoting DVL1 ubiquitination and downregulating the activation of Wnt/ β-catenin signaling
Exp Cell Res. 2024 Feb 15;435(2):113949. doi: 10.1016/j.yexcr.2024.113949. Epub 2024 Jan 23.ABSTRACTHECW1 belongs to ubiquitin ligase (E3) HECT family, and is found to be involved in tumorigenesis and tumor progression. However, the function of HECW1 in cervical cancer (CC) remains unknown. Clinical analysis showed that HECW1 is significantly decreased in CC tumor tissues. Ectopic expression of HECW1 suppressed cell growth, promoting cell cycle arrest and apoptosis in CC cells, while downregulation of HECW1 reversed these trends, impeded proliferation and accelerated cell cycle progression of CC cells. Overexpressing of HE...
Source: Experimental Cell Research - January 24, 2024 Category: Cytology Authors: Zhen Xu Yilin Guo Lu Wang Jinquan Cui Source Type: research
Transcriptional mechanism of E2F1/TFAP2C/NRF1 in regulating KANK2 gene in nephrotic syndrome
In this study, we found that the levels of KANK2 in patients with NS were considerably lower than those in healthy controls, especially in NS patients with acute kidney injury (AKI). To get a deeper understanding of the KANK2 transcriptional control mechanism, the core promoter region of the KANK2 gene was identified. KANK2 was further found to be positively regulated by E2F Transcription Factor 1 (E2F1), Transcription Factor AP-2 Gamma (TFAP2C), and Nuclear Respiratory Factor 1 (NRF1), both at mRNA and protein levels. Knocking down E2F1, TFAP2C, or NRF1 deformed the cytoskeleton of renal tubular cells and reduced F-actin ...
Source: Experimental Cell Research - January 22, 2024 Category: Cytology Authors: Yan Mao Yan Zhou Yan Chen Rong Xu Yi-Qing Wu Wei-Wei Zhu Xu-Fang Wang Qian Wang Chen-Xia Juan Source Type: research
RBM10 regulates the tumorigenic potential of human cancer cells by modulating PPM1B and YBX1 activities
Exp Cell Res. 2024 Feb 15;435(2):113932. doi: 10.1016/j.yexcr.2024.113932. Epub 2024 Jan 20.ABSTRACTRNA binding protein RBM10 participates in various RNA metabolism, and its decreased expression or loss of function by mutation has been identified in many human cancers. However, how its dysregulation contributes to human cancer pathogenesis remains to be determined. Here, we found that RBM10 expression was decreased in breast tumors, and breast cancer patients with low RBM10 expression presented poorer survival rates. RBM10 depletion in breast cancer cells significantly promotes the cellular proliferation and migration. We ...
Source: Experimental Cell Research - January 21, 2024 Category: Cytology Authors: Yueyang Li Di Wei Zixiang Chen Yukun Chen Yuchun Deng Mengge Li Yongliang Zhao Kaifeng Niu Source Type: research
CEBPA facilitates LOXL2 and LOXL3 transcription to promote BCL-2 stability and thus enhances the growth and metastasis of lung carcinoma cells in vitro
In conclusion, CEBPA boosts LOXL2 and LOXL3 transcription to facilitate BCL-2 stability by recruiting Tip60 and thus contributes to LC cell growth and metastasis.PMID:38242344 | DOI:10.1016/j.yexcr.2024.113937 (Source: Experimental Cell Research)
Source: Experimental Cell Research - January 19, 2024 Category: Cytology Authors: Lihua Fan Weijuan Jiang Canjuan Chen Hong Gao Jiangwei Shi Dewen Wang Source Type: research
Hic-5 regulates extracellular matrix-associated gene expression and cytokine secretion in cancer associated fibroblasts
Exp Cell Res. 2024 Feb 15;435(2):113930. doi: 10.1016/j.yexcr.2024.113930. Epub 2024 Jan 17.ABSTRACTThe focal adhesion protein, Hic-5 plays a key role in promoting extracellular matrix deposition and remodeling by cancer associated fibroblasts within the tumor stroma to promote breast tumor cell invasion. However, whether stromal matrix gene expression is regulated by Hic-5 is still unknown. Utilizing a constitutive Hic-5 knockout, Mouse Mammary Tumor Virus-Polyoma Middle T-Antigen spontaneous breast tumor mouse model, bulk RNAseq analysis was performed on cancer associated fibroblasts isolated from Hic-5 knockout mammary ...
Source: Experimental Cell Research - January 18, 2024 Category: Cytology Authors: Weiyi Xu Gregory J Goreczny Ian Forsythe Grant Brennan Theresa Stowell Katia Brock Benjamin Capella Christopher E Turner Source Type: research
Direct differentiation of rat skin fibroblasts into cardiomyocytes
In this study, skin fibroblasts were selected for reprogramming because they serve as a convenient source for the autologous cell therapy. Fibroblasts were isolated from skin of rat pups, propagated, and directly reprogrammed towards cardiac lineage. For reprogramming, two different approaches were adopted, i.e., cells were transfected with: (1) combination of cardiac transcription factors; GATA4, MEF2c, Nkx2.5 (GMN), and (2) combination of cardiac transcription factors; GATA4, MEF2c, Nkx2.5, and iPSC factors; Oct4, Klf4, Sox2 and cMyc (GMNO). After 72 h of transfection, cells were analyzed for the expression of cardiac ma...
Source: Experimental Cell Research - January 18, 2024 Category: Cytology Authors: Waqas Ahmad Bilal Saleh Rida-E-Maria Qazi Rabbia Muneer Irfan Khan Mohsin Khan Asmat Salim Source Type: research
CLSPN actives Wnt/ β-catenin signaling to facilitate glycolysis and cell proliferation in oral squamous cell carcinoma
CONCLUSION: CLSPN promoted glycolysis and OSCC cell proliferation, and reduced apoptosis, which was achieved by the activation of Wnt/β-catenin signaling pathway.PMID:38237848 | DOI:10.1016/j.yexcr.2024.113935 (Source: Experimental Cell Research)
Source: Experimental Cell Research - January 18, 2024 Category: Cytology Authors: Zeyu Hou Chenzhou Wu Jinru Tang Shaohua Liu Longjiang Li Source Type: research
Preparation and in vitro evaluation of cell adhesion and long-term proliferation of stem cells cultured on silibinin co-embedded PLGA/Collagen electrospun composite nanofibers
Exp Cell Res. 2024 Feb 1;435(1):113926. doi: 10.1016/j.yexcr.2024.113926. Epub 2024 Jan 14.ABSTRACTThe present research aims to evaluate the efficacy of Silibinin-loaded mesoporous silica nanoparticles (Sil@MSNs) immobilized into polylactic-co-glycolic acid/Collagen (PLGA/Col) nanofibers on the in vitro proliferation of adipose-derived stem cells (ASCs) and cellular senescence. Here, the fabricated electrospun PLGA/Col composite scaffolds were coated with Sil@MSNs and their physicochemical properties were examined by FTIR, FE-SEM, and TGA. The growth, viability and proliferation of ASCs were investigated using various biol...
Source: Experimental Cell Research - January 16, 2024 Category: Cytology Authors: Mehdi Dadashpour Shaylan Kalavi Amirsasan Gorgzadeh Rahim Nosrati Akram Firouzi Amandi Meysam Mohammadikhah Mehdi Rezai Seghin Sara Effat Alizadeh Source Type: research
