Role of ONECUT family transcription factors in cancer and other diseases
Exp Cell Res. 2024 Apr 7:114035. doi: 10.1016/j.yexcr.2024.114035. Online ahead of print.ABSTRACTMembers of ONECUT transcription factor play an essential role in several developmental processes, however, the atypical expression of ONECUT proteins lead to numerous diseases, including cancer. ONECUT family proteins promote cell proliferation, progression, invasion, metastasis, angiogenesis, and stemness. This family of proteins interacts with other proteins such as KLF4, TGF-β, VEGFA, PRC2, SMAD3 and alters their expression involved in the regulation of various signaling pathways including Jak/Stat3, Akt/Erk, TGF-β, Smad2/...
Source: Experimental Cell Research - April 9, 2024 Category: Cytology Authors: Kumari Sunita Prajapati Sanjay Gupta Smriti Choudhari Shashank Kumar Source Type: research
Development and in-depth characterization of BRAFi-resistant melanoma cell lines in vitro and in vivo
Exp Cell Res. 2024 Apr 7:114033. doi: 10.1016/j.yexcr.2024.114033. Online ahead of print.ABSTRACTRegardless of the clinical response and improved patient survival observed following treatment with BRAFi like Vemurafenib (Vem), rapid development of resistance still remains as a major obstacle in melanoma therapy. In this context, we developed and characterized two acquired Vem-resistant melanoma cell lines, A375V and SK-MEL-28V, and an intrinsically Vem-resistant cell line, RPMI-7951. Altered morphology and growth rate of the resistant cell lines displayed spindle-shaped cells with filopodia formation and enhanced prolifera...
Source: Experimental Cell Research - April 9, 2024 Category: Cytology Authors: Aishwarya Saraswat Ketan Patel Source Type: research
Role of ONECUT family transcription factors in cancer and other diseases
Exp Cell Res. 2024 Apr 7:114035. doi: 10.1016/j.yexcr.2024.114035. Online ahead of print.ABSTRACTMembers of ONECUT transcription factor play an essential role in several developmental processes, however, the atypical expression of ONECUT proteins lead to numerous diseases, including cancer. ONECUT family proteins promote cell proliferation, progression, invasion, metastasis, angiogenesis, and stemness. This family of proteins interacts with other proteins such as KLF4, TGF-β, VEGFA, PRC2, SMAD3 and alters their expression involved in the regulation of various signaling pathways including Jak/Stat3, Akt/Erk, TGF-β, Smad2/...
Source: Experimental Cell Research - April 9, 2024 Category: Cytology Authors: Kumari Sunita Prajapati Sanjay Gupta Smriti Choudhari Shashank Kumar Source Type: research
Salidroside induces mitochondrial dysfunction and ferroptosis to inhibit melanoma progression through reactive oxygen species production
This study used human malignant melanoma cells treated with SAL either alone or in combination with ROS scavenger (NAC) or ferroptosis inducer (Erastin). Through cell viability, wound healing assays, and a Seahorse analyze found that SAL inhibited cell proliferation, migration, extracellular acidification rate, and oxygen consumption rate. Metabolic flux analysis, complexes I, II, III, and IV activity of the mitochondrial respiratory chain assays, mitochondrial membrane potential assay, mitochondrial ROS, and transmission electron microscope revealed that SAL induced mitochondrial dysfunction and ultrastructural damage. As...
Source: Experimental Cell Research - April 8, 2024 Category: Cytology Authors: Xianqi Zhang Mengdi Zhang Ziyan Zhang Shengbo Zhou Source Type: research
Salidroside induces mitochondrial dysfunction and ferroptosis to inhibit melanoma progression through reactive oxygen species production
This study used human malignant melanoma cells treated with SAL either alone or in combination with ROS scavenger (NAC) or ferroptosis inducer (Erastin). Through cell viability, wound healing assays, and a Seahorse analyze found that SAL inhibited cell proliferation, migration, extracellular acidification rate, and oxygen consumption rate. Metabolic flux analysis, complexes I, II, III, and IV activity of the mitochondrial respiratory chain assays, mitochondrial membrane potential assay, mitochondrial ROS, and transmission electron microscope revealed that SAL induced mitochondrial dysfunction and ultrastructural damage. As...
Source: Experimental Cell Research - April 8, 2024 Category: Cytology Authors: Xianqi Zhang Mengdi Zhang Ziyan Zhang Shengbo Zhou Source Type: research
Effects of sevoflurane on lung alveolar epithelial wound healing and survival in a sterile in vitro model of acute respiratory distress syndrome
In this study, we investigated the effects of sevoflurane on lung injury in cultured human carcinoma-derived lung alveolar epithelial (A549) cells. We found that sevoflurane was associated with improved wound healing after exposure to inflammatory cytokines, with preserved cell proliferation but no effect on cell migration properties. Sevoflurane exposure was also associated with enhanced cell viability and active autophagy in A549 cells exposed to cytokines. These findings suggest that sevoflurane may have beneficial effects on lung epithelial injury by promoting alveolar epithelial wound healing and by influencing the su...
Source: Experimental Cell Research - April 7, 2024 Category: Cytology Authors: Florian Loubet C édric Robert Charlotte Leclaire Camille Theilli ère C écile Saint-Béat Woodys Lenga Ma Bonda Ruoyang Zhai R égine Minet-Quinard Corinne Belville Loic Blanchon Vincent Sapin Marc Garnier Matthieu Jabaudon Source Type: research
Single-cell transcriptomics reveals the intra-tumoral heterogeneity and SQSTM1/P62 and Wnt/ β-catenin mediated epithelial to mesenchymal transition and stemness of triple-negative breast cancer
In this study, we have employed single-cell RNA sequencing (scRNA-seq) to dissect the intra-tumoral heterogeneity and analyze the single-cell transcriptomic landscape to detect rare consequential cell subpopulations of significance. The scRNA-seq analysis of TNBC and Normal patient derived samples revealed that EMT markers and transcription factors were most upregulated in MSC population. Further, exploration of gene expression analysis among TNBC and Normal patient-derived MSCs ascertained the role of SQSTM1/P62 and Wnt/β-catenin in TNBC progression. Wnt/β-catenin and Wnt/PCP signaling pathways are prominent contributor...
Source: Experimental Cell Research - April 7, 2024 Category: Cytology Authors: Rajib Shome Plaboni Sen Shilpi Sarkar Siddhartha Sankar Ghosh Source Type: research
Naringenin inhibits APAP-induced acute liver injury through activating PPARA-dependent signaling pathway
In conclusion, this study provides a preliminary theoretical basis for the screening of active compounds in CRP for the prevention and treatment of ALI.PMID:38582338 | DOI:10.1016/j.yexcr.2024.114028 (Source: Experimental Cell Research)
Source: Experimental Cell Research - April 6, 2024 Category: Cytology Authors: Jiepei Xu Jiamin Chen Jinji Deng Xiaojing Chen Rong Du Zhiqian Yu Shuhan Gao Baizhong Chen Yuxin Wang Xiaoting Cai Huiying Duan Yi Cai Guodong Zheng Source Type: research
Naringenin inhibits APAP-induced acute liver injury through activating PPARA-dependent signaling pathway
In conclusion, this study provides a preliminary theoretical basis for the screening of active compounds in CRP for the prevention and treatment of ALI.PMID:38582338 | DOI:10.1016/j.yexcr.2024.114028 (Source: Experimental Cell Research)
Source: Experimental Cell Research - April 6, 2024 Category: Cytology Authors: Jiepei Xu Jiamin Chen Jinji Deng Xiaojing Chen Rong Du Zhiqian Yu Shuhan Gao Baizhong Chen Yuxin Wang Xiaoting Cai Huiying Duan Yi Cai Guodong Zheng Source Type: research
PD-1 and LAG-3 positive T cells are related with the prognosis of patients with chronic kidney disease
CONCLUSION: In summary, the present research showed that the frequencies of LAG-3+ and PD-1+ T cells were remarkably enhanced in CKD patients. These findings offer novel insights and potential therapeutic targets for the management of CKD.PMID:38574959 | DOI:10.1016/j.yexcr.2024.114027 (Source: Experimental Cell Research)
Source: Experimental Cell Research - April 4, 2024 Category: Cytology Authors: Hongwei Jiang Jing Wu Junlin Zhang Source Type: research
PD-1 and LAG-3 positive T cells are related with the prognosis of patients with chronic kidney disease
CONCLUSION: In summary, the present research showed that the frequencies of LAG-3+ and PD-1+ T cells were remarkably enhanced in CKD patients. These findings offer novel insights and potential therapeutic targets for the management of CKD.PMID:38574959 | DOI:10.1016/j.yexcr.2024.114027 (Source: Experimental Cell Research)
Source: Experimental Cell Research - April 4, 2024 Category: Cytology Authors: Hongwei Jiang Jing Wu Junlin Zhang Source Type: research
PD-1 and LAG-3 positive T cells are related with the prognosis of patients with chronic kidney disease
CONCLUSION: In summary, the present research showed that the frequencies of LAG-3+ and PD-1+ T cells were remarkably enhanced in CKD patients. These findings offer novel insights and potential therapeutic targets for the management of CKD.PMID:38574959 | DOI:10.1016/j.yexcr.2024.114027 (Source: Experimental Cell Research)
Source: Experimental Cell Research - April 4, 2024 Category: Cytology Authors: Hongwei Jiang Jing Wu Junlin Zhang Source Type: research
A miRNA-7704/IL2RB/AKT feedback loop regulates tumorigenesis and chemoresistance in ovarian cancer
In this study, we found that miR-7704 inhibited the proliferation and promoted cisplatin sensitivity of ovarian cancer cells in vitro and in vivo. Moreover, ectopic expression of miR-7704 had the same effect as IL2RB knockdown. Further mechanistic studies revealed that miR-7704 played an inhibitory role by regulating IL2RB expression to inactivate the AKT signaling pathway. Furthermore, IL2RB reversed the miR-7704 mediated resistance to cisplatin in ovarian cancer. Based on these findings, miR-7704 and IL2RB show the potential as novel therapeutic targets for ovarian cancer.PMID:38565343 | DOI:10.1016/j.yexcr.2024.114012 (...
Source: Experimental Cell Research - April 2, 2024 Category: Cytology Authors: Xuan Meng Xiaoqing Liang Shengjie Yang Dongsheng Wu Xinghe Wang Source Type: research
A miRNA-7704/IL2RB/AKT feedback loop regulates tumorigenesis and chemoresistance in ovarian cancer
In this study, we found that miR-7704 inhibited the proliferation and promoted cisplatin sensitivity of ovarian cancer cells in vitro and in vivo. Moreover, ectopic expression of miR-7704 had the same effect as IL2RB knockdown. Further mechanistic studies revealed that miR-7704 played an inhibitory role by regulating IL2RB expression to inactivate the AKT signaling pathway. Furthermore, IL2RB reversed the miR-7704 mediated resistance to cisplatin in ovarian cancer. Based on these findings, miR-7704 and IL2RB show the potential as novel therapeutic targets for ovarian cancer.PMID:38565343 | DOI:10.1016/j.yexcr.2024.114012 (...
Source: Experimental Cell Research - April 2, 2024 Category: Cytology Authors: Xuan Meng Xiaoqing Liang Shengjie Yang Dongsheng Wu Xinghe Wang Source Type: research
The CNS microenvironment promotes leukemia cell survival by disrupting tumor suppression and cell cycle regulation in pediatric T-cell acute lymphoblastic leukemia
Exp Cell Res. 2024 Mar 30:114015. doi: 10.1016/j.yexcr.2024.114015. Online ahead of print.ABSTRACTA major obstacle in improving survival in pediatric T-cell acute lymphoblastic leukemia is understanding how to predict and treat leukemia relapse in the CNS. Leukemia cells are capable of infiltrating and residing within the CNS, primarily the leptomeninges, where they interact with the microenvironment and remain sheltered from systemic treatment. These cells can survive in the CNS, by hijacking the microenvironment and disrupting normal functions, thus promoting malignant transformation. While the protective effects of the ...
Source: Experimental Cell Research - April 1, 2024 Category: Cytology Authors: Sabina Enlund Indranil Sinha Christina Neofytou Amanda Ramilo Amor Konstantinos Papadakis Anna Nilsson Qingfei Jiang Ola Hermanson Frida Holm Source Type: research
