CLSPN actives Wnt/ β-catenin signaling to facilitate glycolysis and cell proliferation in oral squamous cell carcinoma
CONCLUSION: CLSPN promoted glycolysis and OSCC cell proliferation, and reduced apoptosis, which was achieved by the activation of Wnt/β-catenin signaling pathway.PMID:38237848 | DOI:10.1016/j.yexcr.2024.113935 (Source: Experimental Cell Research)
Source: Experimental Cell Research - January 18, 2024 Category: Cytology Authors: Zeyu Hou Chenzhou Wu Jinru Tang Shaohua Liu Longjiang Li Source Type: research

Preparation and in vitro evaluation of cell adhesion and long-term proliferation of stem cells cultured on silibinin co-embedded PLGA/Collagen electrospun composite nanofibers
Exp Cell Res. 2024 Feb 1;435(1):113926. doi: 10.1016/j.yexcr.2024.113926. Epub 2024 Jan 14.ABSTRACTThe present research aims to evaluate the efficacy of Silibinin-loaded mesoporous silica nanoparticles (Sil@MSNs) immobilized into polylactic-co-glycolic acid/Collagen (PLGA/Col) nanofibers on the in vitro proliferation of adipose-derived stem cells (ASCs) and cellular senescence. Here, the fabricated electrospun PLGA/Col composite scaffolds were coated with Sil@MSNs and their physicochemical properties were examined by FTIR, FE-SEM, and TGA. The growth, viability and proliferation of ASCs were investigated using various biol...
Source: Experimental Cell Research - January 16, 2024 Category: Cytology Authors: Mehdi Dadashpour Shaylan Kalavi Amirsasan Gorgzadeh Rahim Nosrati Akram Firouzi Amandi Meysam Mohammadikhah Mehdi Rezai Seghin Sara Effat Alizadeh Source Type: research

Corrigendum to "Downregulation of CYRI-B promotes migration, invasion and EMT by activating the Rac1-STAT3 pathway in gastric cancer" [423(2023)/113453]
Exp Cell Res. 2024 Feb 1;435(1):113890. doi: 10.1016/j.yexcr.2023.113890. Epub 2024 Jan 12.NO ABSTRACTPMID:38217935 | DOI:10.1016/j.yexcr.2023.113890 (Source: Experimental Cell Research)
Source: Experimental Cell Research - January 13, 2024 Category: Cytology Authors: Guangyao Liu Yujian Xia Huijin Wang Xinghan Jin Songyao Chen Wei Chen Changhua Zhang Yulong He Source Type: research

MiR-92a-2-5p suppresses esophageal squamous cell carcinoma cell proliferation and invasion by targeting PRDX2
Exp Cell Res. 2024 Feb 1;435(1):113925. doi: 10.1016/j.yexcr.2024.113925. Epub 2024 Jan 9.ABSTRACTMicroRNAs (miRNAs) can function as negative regulators of gene expression by binding to the 3'-untranslated region (3'-UTR) of target genes. The aberrant expression of miRNAs in neoplasm is extensively associated with tumorigenesis and cancer progression, including esophageal squamous cell carcinoma (ESCC). Our previous investigation has identified the oncogenic roles of Peroxiredoxin2 (PRDX2) in ESCC progression; however, its upstream regulatory mechanism remains to be elucidated. By merging the prediction results from miRWal...
Source: Experimental Cell Research - January 11, 2024 Category: Cytology Authors: Xiang Zhan Jixian Li Renya Zeng Lingli Lei Alei Feng Zhe Yang Source Type: research

MAPRE3 as an epigenetic target of EZH2 restricts ovarian cancer proliferation in vitro and in vivo
Exp Cell Res. 2024 Feb 1;435(1):113913. doi: 10.1016/j.yexcr.2024.113913. Epub 2024 Jan 8.ABSTRACTOvarian cancer (OC) is a lethal gynecologic cancer and the common cause of death within women worldwide. The polycomb group protein enhancer of zeste homolog 2 (EZH2) is a histone methyltransferase highly expressed in various tumors, including OC. However, the mechanistic basis of EZH2 oncogenic activity in OC remain incompletely understood. Bioinformatics analysis showed that the expression of MAPRE3 was lower in OC tissues than in normal tissues, and was positively correlated with the overall survival. MAPRE3 overexpression ...
Source: Experimental Cell Research - January 10, 2024 Category: Cytology Authors: Yun Zhang Fanglin Liu Jindan Zheng Keping Jiang Hao Ai Lili Liu Dong Mao Source Type: research

CAMSAP3, a microtubule orientation regulator, plays a vital role in manifesting differentiation-dependent characteristics in keratinocytes
In this study, we identified microtubule-bound CAMSAP3 within HaCaT keratinocytes, revealing an upregulation during the mitotic phase and accumulation at the intercellular bridge during cytokinesis. Building upon this observation, we scrutinized cellular responses upon a tetracycline/doxycycline-inducible CAMSAP3 expression in CAMSAP3-deficient HaCaT cells. Remarkably, CAMSAP3 deficiency induced shifts in microtubule orientation, resulting in cell cycle exit and delayed cytokinesis in a subset of the cells. Furthermore, our inquiry unveiled that CAMSAP3 deficiency adversely impacted the formation and stability of Adherens ...
Source: Experimental Cell Research - January 8, 2024 Category: Cytology Authors: Mako Kobori Junya Abe Reika Saito Yohei Hirai Source Type: research

circTldc1 increases Tldc1 expression by targeting miR-485-5p to promote fibroblast-like synoviocytes proliferation in collagen-induced arthritis
In this study, we elucidated the expression profile of circRNA in FLSs, as well as the role and molecular mechanisms of circTldc1. Through sequencing and validation experiments on primary FLSs derived from collagen-induced arthritis (CIA) rats, we found that circTldc1 can promote FLSs proliferation and exacerbate CIA-induced joint damage. The data revealed that circTldc1's parent gene, Tldc1, is homologous to human Tldc1, and circTldc1 is located in the cytoplasm of FLSs, belonging to the exonic circRNA category. The results from bioinformatics analysis, molecular experiments on FLSs (manipulating circTldc1 expression in v...
Source: Experimental Cell Research - January 8, 2024 Category: Cytology Authors: Xiaoyu Cai Yao Yao Fujia Ren Shiwei Zhang Source Type: research

A novel tsRNA-5008a promotes ferroptosis in cardiomyocytes that causes atrial structural remodeling predisposed to atrial fibrillation
Exp Cell Res. 2024 Feb 15;435(2):113923. doi: 10.1016/j.yexcr.2024.113923. Epub 2024 Jan 6.ABSTRACTAtrial fibrillation (AF) is an extremely common clinical arrhythmia disease, but whether its mechanism is associated with ferroptosis remains unclear. The tRNA-derived small RNAs (tsRNAs) are involved in a variety of cardiovascular diseases, however, their role and mechanism in atrial remodeling in AF have not been studied. We aimed to explore whether tsRNAs mediate ferroptosis in AF progression. The AF models were constructed to detect ferroptosis-related indicators, and Ferrostatin-1 (Fer-1) was introduced to clarify the re...
Source: Experimental Cell Research - January 8, 2024 Category: Cytology Authors: Liangzhen Xie Zewei Zhao Hao Xia Shuang Su Liwei He Zhaohui Huang Yongchun Li Mingjian Gao Jun Chen Jian Peng Yunjun Ruan Source Type: research

CAMSAP3, a microtubule orientation regulator, plays a vital role in manifesting differentiation-dependent characteristics in keratinocytes
In this study, we identified microtubule-bound CAMSAP3 within HaCaT keratinocytes, revealing an upregulation during the mitotic phase and accumulation at the intercellular bridge during cytokinesis. Building upon this observation, we scrutinized cellular responses upon a tetracycline/doxycycline-inducible CAMSAP3 expression in CAMSAP3-deficient HaCaT cells. Remarkably, CAMSAP3 deficiency induced shifts in microtubule orientation, resulting in cell cycle exit and delayed cytokinesis in a subset of the cells. Furthermore, our inquiry unveiled that CAMSAP3 deficiency adversely impacted the formation and stability of Adherens ...
Source: Experimental Cell Research - January 8, 2024 Category: Cytology Authors: Mako Kobori Junya Abe Reiko Saito Yohei Hirai Source Type: research

circTldc1 increases Tldc1 expression by targeting miR-485-5p to promote fibroblast-like synoviocytes proliferation in collagen-induced arthritis
In this study, we elucidated the expression profile of circRNA in FLSs, as well as the role and molecular mechanisms of circTldc1. Through sequencing and validation experiments on primary FLSs derived from collagen-induced arthritis (CIA) rats, we found that circTldc1 can promote FLSs proliferation and exacerbate CIA-induced joint damage. The data revealed that circTldc1's parent gene, Tldc1, is homologous to human Tldc1, and circTldc1 is located in the cytoplasm of FLSs, belonging to the exonic circRNA category. The results from bioinformatics analysis, molecular experiments on FLSs (manipulating circTldc1 expression in v...
Source: Experimental Cell Research - January 8, 2024 Category: Cytology Authors: Xiaoyu Cai Yao Yao Fujia Ren Shiwei Zhang Source Type: research

A novel tsRNA-5008a promotes ferroptosis in cardiomyocytes that causes atrial structural remodeling predisposed to atrial fibrillation
Exp Cell Res. 2024 Jan 6:113923. doi: 10.1016/j.yexcr.2024.113923. Online ahead of print.ABSTRACTAtrial fibrillation (AF) is an extremely common clinical arrhythmia disease, but whether its mechanism is associated with ferroptosis remains unclear. The tRNA-derived small RNAs (tsRNAs) are involved in a variety of cardiovascular diseases, however, their role and mechanism in atrial remodeling in AF have not been studied. We aimed to explore whether tsRNAs mediate ferroptosis in AF progression. The AF models were constructed to detect ferroptosis-related indicators, and Ferrostatin-1 (Fer-1) was introduced to clarify the rela...
Source: Experimental Cell Research - January 8, 2024 Category: Cytology Authors: Liangzhen Xie Zewei Zhao Hao Xia Shuang Su Liwei He Zhaohui Huang Yongchun Li Mingjian Gao Jun Chen Jian Peng Yunjun Ruan Source Type: research

CAMSAP3, a microtubule orientation regulator, plays a vital role in manifesting differentiation-dependent characteristics in keratinocytes
In this study, we identified microtubule-bound CAMSAP3 within HaCaT keratinocytes, revealing an upregulation during the mitotic phase and accumulation at the intercellular bridge during cytokinesis. Building upon this observation, we scrutinized cellular responses upon a tetracycline/doxycycline-inducible CAMSAP3 expression in CAMSAP3-deficient HaCaT cells. Remarkably, CAMSAP3 deficiency induced shifts in microtubule orientation, resulting in cell cycle exit and delayed cytokinesis in a subset of the cells. Furthermore, our inquiry unveiled that CAMSAP3 deficiency adversely impacted the formation and stability of Adherens ...
Source: Experimental Cell Research - January 8, 2024 Category: Cytology Authors: Mako Kobori Junya Abe Reiko Saito Yohei Hirai Source Type: research

circTldc1 increases Tldc1 expression by targeting miR-485-5p to promote fibroblast-like synoviocytes proliferation in collagen-induced arthritis
In this study, we elucidated the expression profile of circRNA in FLSs, as well as the role and molecular mechanisms of circTldc1. Through sequencing and validation experiments on primary FLSs derived from collagen-induced arthritis (CIA) rats, we found that circTldc1 can promote FLSs proliferation and exacerbate CIA-induced joint damage. The data revealed that circTldc1's parent gene, Tldc1, is homologous to human Tldc1, and circTldc1 is located in the cytoplasm of FLSs, belonging to the exonic circRNA category. The results from bioinformatics analysis, molecular experiments on FLSs (manipulating circTldc1 expression in v...
Source: Experimental Cell Research - January 8, 2024 Category: Cytology Authors: Xiaoyu Cai Yao Yao Fujia Ren Shiwei Zhang Source Type: research

A novel tsRNA-5008a promotes ferroptosis in cardiomyocytes that causes atrial structural remodeling predisposed to atrial fibrillation
Exp Cell Res. 2024 Jan 6:113923. doi: 10.1016/j.yexcr.2024.113923. Online ahead of print.ABSTRACTAtrial fibrillation (AF) is an extremely common clinical arrhythmia disease, but whether its mechanism is associated with ferroptosis remains unclear. The tRNA-derived small RNAs (tsRNAs) are involved in a variety of cardiovascular diseases, however, their role and mechanism in atrial remodeling in AF have not been studied. We aimed to explore whether tsRNAs mediate ferroptosis in AF progression. The AF models were constructed to detect ferroptosis-related indicators, and Ferrostatin-1 (Fer-1) was introduced to clarify the rela...
Source: Experimental Cell Research - January 8, 2024 Category: Cytology Authors: Liangzhen Xie Zewei Zhao Hao Xia Shuang Su Liwei He Zhaohui Huang Yongchun Li Mingjian Gao Jun Chen Jian Peng Yunjun Ruan Source Type: research

Nuclear-cytoplasmic translocation of SQSTM1/p62 protein enhances ESCC cell migration and invasion by stabilizing EPLIN expression
This study aims to clarify the impacts of different cellular localization of p62 on the function of ESCC cells and the underlying molecular mechanisms. We here demonstrated that cytoplasmic p62 enhances the migration and invasion abilities of esophageal cancer cells, whereas nuclear p62 has no effect. We further explored the interaction protein of p62 by using GST pull-down experiment and identified EPLIN as a potential protein interacting with p62. In addition, reducing EPLIN expression significantly inhibited the migration and invasion of ESCC cells, which were rescued when EPLIN expression was restored after the p62 kno...
Source: Experimental Cell Research - January 7, 2024 Category: Cytology Authors: Zou Liu Li-Yan Yang Jia-Jie Hao Na Zhang Zhi-Lu Fan Hong-Qing Cai Yan Cai Wen-Qiang Wei Yu Zhang Ming-Rong Wang Source Type: research