DPP-4 exacerbates LPS-induced endothelial cells inflammation via integrin- α5β1/FAK/AKT signaling
In this study, the role of DPP-4 on lipopolysaccharide (LPS)-induced pulmonary microvascular endothelial cells (HPMECs) dysfunction and the underlying mechanism were investigated by siRNA-mediated knockdown of DPP-4. Our results indicated that LPS (1 μg/ml) challenge resulted in either the production and releasing of DPP-4, as well as the secretion of IL-6 and IL-8 in HPMECs. DPP-4 knockdown inhibited chemokine releasing and monolayer hyper-permeability in LPS challenged HPMECs. When cocultured with human polymorphonuclear neutrophils (PMNs), DPP4 knockdown suppressed LPS-induced neutrophil-endothelial adhesion, PMN chemo...
Source: Experimental Cell Research - January 6, 2024 Category: Cytology Authors: Chang Liu Jian Xu Jiahao Fan Chenyang Liu Weiping Xie Hui Kong Source Type: research
Exploring the in vivo anti-cancer potential of Neosetophomone B in leukemic cells using a zebrafish xenograft model
Exp Cell Res. 2024 Jan 4:113907. doi: 10.1016/j.yexcr.2023.113907. Online ahead of print.ABSTRACTNeosetophomone B (NSP-B) is a unique meroterpenoid fungal secondary metabolite that has previously demonstrated promising anti-cancer properties against various cancer cell lines in vitro. However, its in vivo anti-cancer potential remaines unexplored. To fill this gap in our knowledge, we tested NSP-B's in vivo anti-cancer activity using a zebrafish model, an organism that has gained significant traction in biomedical research due to its genetic similarities with humans and its transparent nature, allowing real-time tumor grow...
Source: Experimental Cell Research - January 6, 2024 Category: Cytology Authors: Shilpa Kuttikrishnan Maram Hasan Kirti S Prabhu Tamam El-Elimat Nicholas H Oberlies Cedric J Pearce Feras Q Alali Aamir Ahmad Ehsan P Daryakenari Ajaz A Bhat Huseyin C Yalcin Shahab Uddin Source Type: research
Stroma Modulation of radiation response in head and neck squamous cell carcinoma: Insights from zebrafish larvae xenografts
CONCLUSION: This is the first report of co-culturing cancer cells with several types of stroma cells using a zebrafish xenograft model. This study also highlighted the role of stroma cells in turning the cancer cells from radioresponsive to radioresistant.PMID:38182078 | DOI:10.1016/j.yexcr.2024.113911 (Source: Experimental Cell Research)
Source: Experimental Cell Research - January 5, 2024 Category: Cytology Authors: Wafa Wahbi Shady Awad Tuula Salo Ahmed Al-Samadi Source Type: research
Glycyrrhizin attenuates myocardial ischemia reperfusion injury by suppressing Inflammation, oxidative stress, and ferroptosis via the HMGB1-TLR4-GPX4 pathway
In this study, SD rats underwent in vivo myocardial ischemia/reperfusion (I/R) surgery, while H9C2 cells were subjected to the hypoxia/reoxygenation (H/R) model for in vitro experiments. In addition, TAK-242, a TLR4-specific antagonist, and GL were also used to evaluate the effect and mechanisms of GL on the cardiac function and expression of ferroptosis-related gene and protein in vivo and vitro. The results show that GL decreased not only the expression of the inflammation-related factors (HMGB1, TNF-α, IL-6, IL-18 and IL-1β), but also reduced the number of TUNEL-positive cardiomyocytes, and mitigated pathological alte...
Source: Experimental Cell Research - January 4, 2024 Category: Cytology Authors: Kaiyi Zhu Rong Fan Yuchen Cao Wei Yang Zhe Zhang Qiang Zhou Jie Ren Xiushan Shi Yuping Gao Xiang Guo Source Type: research
Caveolae-mediated endocytosis of extracellular QSOX1b modulates the migration of fibroblasts
Exp Cell Res. 2024 Jan 2;435(1):113906. doi: 10.1016/j.yexcr.2023.113906. Online ahead of print.ABSTRACTQuiescin/sulfhydryl oxidase (QSOX1) is a secreted flavoprotein that modulates cellular proliferation, migration and adhesion, roles attributed to its ability to organize the extracellular matrix. We previously showed that exogenously added QSOX1b induces smooth muscle cells migration in a process that depends on its enzymatic activity and that is mediated by hydrogen peroxide derived from Nox1, a catalytic subunit of NAD(P)H oxidases. Here, we report that exogenous QSOX1b also stimulates the migration of L929 fibroblasts...
Source: Experimental Cell Research - January 4, 2024 Category: Cytology Authors: Pierina A Martinez Silvio M Zanata Lia S Nakao Source Type: research
Glycyrrhizin attenuates myocardial ischemia reperfusion injury by suppressing Inflammation, oxidative stress, and ferroptosis via the HMGB1-TLR4-GPX4 pathway
In this study, SD rats underwent in vivo myocardial ischemia/reperfusion (I/R) surgery, while H9C2 cells were subjected to the hypoxia/reoxygenation (H/R) model for in vitro experiments. In addition, TAK-242, a TLR4-specific antagonist, and GL were also used to evaluate the effect and mechanisms of GL on the cardiac function and expression of ferroptosis-related gene and protein in vivo and vitro. The results show that GL decreased not only the expression of the inflammation-related factors (HMGB1, TNF-α, IL-6, IL-18 and IL-1β), but also reduced the number of TUNEL-positive cardiomyocytes, and mitigated pathological alte...
Source: Experimental Cell Research - January 4, 2024 Category: Cytology Authors: Kaiyi Zhu Rong Fan Yuchen Cao Wei Yang Zhe Zhang Qiang Zhou Jie Ren Xiushan Shi Yuping Gao Xiang Guo Source Type: research
Caveolae-mediated endocytosis of extracellular QSOX1b modulates the migration of fibroblasts
Exp Cell Res. 2024 Jan 2:113906. doi: 10.1016/j.yexcr.2023.113906. Online ahead of print.ABSTRACTQuiescin/sulfhydryl oxidase (QSOX1) is a secreted flavoprotein that modulates cellular proliferation, migration and adhesion, roles attributed to its ability to organize the extracellular matrix. We previously showed that exogenously added QSOX1b induces smooth muscle cells migration in a process that depends on its enzymatic activity and that is mediated by hydrogen peroxide derived from Nox1, a catalytic subunit of NAD(P)H oxidases. Here, we report that exogenous QSOX1b also stimulates the migration of L929 fibroblasts and th...
Source: Experimental Cell Research - January 4, 2024 Category: Cytology Authors: Pierina A Martinez Silvio M Zanata Lia S Nakao Source Type: research
SP1-stimulated mir-208a-5p aggravates sepsis-induced myocardial injury via targeting XIAP
Exp Cell Res. 2023 Dec 30:113905. doi: 10.1016/j.yexcr.2023.113905. Online ahead of print.ABSTRACTThe development of sepsis can lead to many organ dysfunction and even death. Myocardial injury is one of the serious complications of sepsis leading to death. New evidence suggests that microRNAs (miRNAs) play a critical role in infection myocardial injury. However, the mechanism which miR-208a-5p regulates sepsis-induced myocardial injury remains unclear. To mimic sepsis-induced myocardial injury in vitro, rat primary cardiomyocytes were treated with LPS. Cell viability and apoptosis were tested by CCK-8 and flow cytometry, r...
Source: Experimental Cell Research - January 1, 2024 Category: Cytology Authors: Ling-Jun Xu Yixian Yang Ling-Feng Yuan Hong Liu Nan-Ping Xu Yu Yang Liang Huang Source Type: research
Antitumor efficacy of synthesized Ag-Au nanocomposite loaded with PEG and ascorbic acid in human lung cancer stem cells
In this study we have synthesized silver gold nanocomposite (Ag-Au NC) using polyethylene glycol and l-ascorbic acid as surfactant and reducing agent respectively. Synthesized nanocomposite was characterized by ultraviolet-visible absorption, dynamic light scattering, scanning and transmission electron microscopy. Compositional analysis was carried out by energy dispersive X-ray analysis and average pore diameter was estimated using Barrett-Joyner-Halenda method. In-silico molecular docking analysis of the synthesized NC against active regions of epidermal growth factor receptor revealed good binding energy. Subsequently, ...
Source: Experimental Cell Research - January 1, 2024 Category: Cytology Authors: Gulam Abbas Tajindra Singh Saluja Dharmendra Kumar Hemant Agrawal Anurag Gupta Gajanan Panday Satyendra Kumar Singh Source Type: research
GPR55 inhibits the pro-adipogenic activity of anandamide in human adipose stromal cells
We examined the receptor-expressions by immunostaining on human ASCs and tested their functionality by measuring the expression of immediate early genes (IEGs) related to the transcription factor-complex AP-1 upon exposition to receptor agonists. Cells were stimulated with increasing concentrations of specific ligands to investigate the effects on ASC viability (proliferation and metabolic activity), secretory activity, and AEA mediated differentiation. ASCs expressed both receptors, and their activation suppressed IEG expression. TRPV1 did not affect viability or cytokine secretion. GPR55 decreased proliferation, and it i...
Source: Experimental Cell Research - January 1, 2024 Category: Cytology Authors: Tim Ruhl Aigul Nuptybayeva Bong-Sung Kim Justus P Beier Source Type: research
SP1-stimulated mir-208a-5p aggravates sepsis-induced myocardial injury via targeting XIAP
Exp Cell Res. 2023 Dec 30:113905. doi: 10.1016/j.yexcr.2023.113905. Online ahead of print.ABSTRACTThe development of sepsis can lead to many organ dysfunction and even death. Myocardial injury is one of the serious complications of sepsis leading to death. New evidence suggests that microRNAs (miRNAs) play a critical role in infection myocardial injury. However, the mechanism which miR-208a-5p regulates sepsis-induced myocardial injury remains unclear. To mimic sepsis-induced myocardial injury in vitro, rat primary cardiomyocytes were treated with LPS. Cell viability and apoptosis were tested by CCK-8 and flow cytometry, r...
Source: Experimental Cell Research - January 1, 2024 Category: Cytology Authors: Ling-Jun Xu Yixian Yang Ling-Feng Yuan Hong Liu Nan-Ping Xu Yu Yang Liang Huang Source Type: research
Antitumor efficacy of synthesized Ag-Au nanocomposite loaded with PEG and ascorbic acid in human lung cancer stem cells
In this study we have synthesized silver gold nanocomposite (Ag-Au NC) using polyethylene glycol and l-ascorbic acid as surfactant and reducing agent respectively. Synthesized nanocomposite was characterized by ultraviolet-visible absorption, dynamic light scattering, scanning and transmission electron microscopy. Compositional analysis was carried out by energy dispersive X-ray analysis and average pore diameter was estimated using Barrett-Joyner-Halenda method. In-silico molecular docking analysis of the synthesized NC against active regions of epidermal growth factor receptor revealed good binding energy. Subsequently, ...
Source: Experimental Cell Research - January 1, 2024 Category: Cytology Authors: Gulam Abbas Tajindra Singh Saluja Dharmendra Kumar Hemant Agrawal Anurag Gupta Gajanan Panday Satyendra Kumar Singh Source Type: research
GPR55 inhibits the pro-adipogenic activity of anandamide in human adipose stromal cells
We examined the receptor-expressions by immunostaining on human ASCs and tested their functionality by measuring the expression of immediate early genes (IEGs) related to the transcription factor-complex AP-1 upon exposition to receptor agonists. Cells were stimulated with increasing concentrations of specific ligands to investigate the effects on ASC viability (proliferation and metabolic activity), secretory activity, and AEA mediated differentiation. ASCs expressed both receptors, and their activation suppressed IEG expression. TRPV1 did not affect viability or cytokine secretion. GPR55 decreased proliferation, and it i...
Source: Experimental Cell Research - January 1, 2024 Category: Cytology Authors: Tim Ruhl Aigul Nuptybayeva Bong-Sung Kim Justus P Beier Source Type: research
SP1-stimulated mir-208a-5p aggravates sepsis-induced myocardial injury via targeting XIAP
Exp Cell Res. 2023 Dec 30:113905. doi: 10.1016/j.yexcr.2023.113905. Online ahead of print.ABSTRACTThe development of sepsis can lead to many organ dysfunction and even death. Myocardial injury is one of the serious complications of sepsis leading to death. New evidence suggests that microRNAs (miRNAs) play a critical role in infection myocardial injury. However, the mechanism which miR-208a-5p regulates sepsis-induced myocardial injury remains unclear. To mimic sepsis-induced myocardial injury in vitro, rat primary cardiomyocytes were treated with LPS. Cell viability and apoptosis were tested by CCK-8 and flow cytometry, r...
Source: Experimental Cell Research - January 1, 2024 Category: Cytology Authors: Ling-Jun Xu Yixian Yang Ling-Feng Yuan Hong Liu Nan-Ping Xu Yu Yang Liang Huang Source Type: research
Antitumor efficacy of synthesized Ag-Au nanocomposite loaded with PEG and ascorbic acid in human lung cancer stem cells
In this study we have synthesized silver gold nanocomposite (Ag-Au NC) using polyethylene glycol and l-ascorbic acid as surfactant and reducing agent respectively. Synthesized nanocomposite was characterized by ultraviolet-visible absorption, dynamic light scattering, scanning and transmission electron microscopy. Compositional analysis was carried out by energy dispersive X-ray analysis and average pore diameter was estimated using Barrett-Joyner-Halenda method. In-silico molecular docking analysis of the synthesized NC against active regions of epidermal growth factor receptor revealed good binding energy. Subsequently, ...
Source: Experimental Cell Research - January 1, 2024 Category: Cytology Authors: Gulam Abbas Tajindra Singh Saluja Dharmendra Kumar Hemant Agrawal Anurag Gupta Gajanan Panday Satyendra Kumar Singh Source Type: research
