Lesions of KNDy and Kiss1R neurons in the arcuate nucleus produce different effects on LH pulse patterns in female sheep
This study tested the functional role of ovine KNDy neurons in pulse generation and explored the roles of nearby Kiss1 receptor (Kiss1R)-containing cells using lesions produced with saporin (SAP) conjugates. Injection of NK3-SAP ablated over 90% of the KNDy cells, while Kiss-SAP (saporin conjugated to kisspeptin-54) lesioned about two-thirds of the Kiss1R population without affecting KNDy or GnRH cell number. Both lesions produced a dramatic decrease in LH pulse amplitude, but had different effects on LH pulse patterns. NK3-SAP increased interpulse interval (IPI), but Kiss-SAP did not. In contrast, Kiss-SAP disrupted the r...
Source: Endocrinology - September 30, 2023 Category: Endocrinology Authors: Robert L Goodman Aleisha M Moore Kayla Onslow Stanley M Hileman Steve L Hardy Elizabeth C Bowdridge Burgundy A Walters Sami Agus Max J Griesgraber Eliana G Aerts Michael N Lehman Lique M Coolen Source Type: research
Lesions of KNDy and Kiss1R neurons in the arcuate nucleus produce different effects on LH pulse patterns in female sheep
This study tested the functional role of ovine KNDy neurons in pulse generation and explored the roles of nearby Kiss1 receptor (Kiss1R)-containing cells using lesions produced with saporin (SAP) conjugates. Injection of NK3-SAP ablated over 90% of the KNDy cells, while Kiss-SAP (saporin conjugated to kisspeptin-54) lesioned about two-thirds of the Kiss1R population without affecting KNDy or GnRH cell number. Both lesions produced a dramatic decrease in LH pulse amplitude, but had different effects on LH pulse patterns. NK3-SAP increased interpulse interval (IPI), but Kiss-SAP did not. In contrast, Kiss-SAP disrupted the r...
Source: Endocrinology - September 30, 2023 Category: Endocrinology Authors: Robert L Goodman Aleisha M Moore Kayla Onslow Stanley M Hileman Steve L Hardy Elizabeth C Bowdridge Burgundy A Walters Sami Agus Max J Griesgraber Eliana G Aerts Michael N Lehman Lique M Coolen Source Type: research
Lesions of KNDy and Kiss1R neurons in the arcuate nucleus produce different effects on LH pulse patterns in female sheep
This study tested the functional role of ovine KNDy neurons in pulse generation and explored the roles of nearby Kiss1 receptor (Kiss1R)-containing cells using lesions produced with saporin (SAP) conjugates. Injection of NK3-SAP ablated over 90% of the KNDy cells, while Kiss-SAP (saporin conjugated to kisspeptin-54) lesioned about two-thirds of the Kiss1R population without affecting KNDy or GnRH cell number. Both lesions produced a dramatic decrease in LH pulse amplitude, but had different effects on LH pulse patterns. NK3-SAP increased interpulse interval (IPI), but Kiss-SAP did not. In contrast, Kiss-SAP disrupted the r...
Source: Endocrinology - September 30, 2023 Category: Endocrinology Authors: Robert L Goodman Aleisha M Moore Kayla Onslow Stanley M Hileman Steve L Hardy Elizabeth C Bowdridge Burgundy A Walters Sami Agus Max J Griesgraber Eliana G Aerts Michael N Lehman Lique M Coolen Source Type: research
Legacy and emerging per- and polyfluoroalkyl substances (PFAS) regulate steroidogenesis in the male gonad
Endocrinology. 2023 Sep 28:bqad142. doi: 10.1210/endocr/bqad142. Online ahead of print.ABSTRACTPer- and polyfluoroalkyl substances (PFAS) are widely used in a variety of industrial processes and manufacturing of consumer products. Current efforts by the manufacturing industry will limit use of long-chain or legacy PFAS represented by perfluorooctanoic acid (PFOA) and perfluorooctane sulfonic acid (PFOS) and replace with short-chain or emerging PFAS such as perfluorobutanoic acid (PFBA) and perfluorobutane sulfonic acid (PFBS). However, there is little to no information on the toxicity of new and emerging PFAS. Therefore, w...
Source: Endocrinology - September 28, 2023 Category: Endocrinology Authors: Samantha Daugherty Vanisree Mulabagal Joel Hayworth Benson T Akingbemi Source Type: research
Functional Water Channels Within the TSH Receptor - a new paradigm for TSH action with disease implications
Endocrinology. 2023 Sep 28:bqad146. doi: 10.1210/endocr/bqad146. Online ahead of print.ABSTRACTThe TSH receptor (TSHR) transmembrane domain (TMD) lives in the plasma membrane consisting of lipids and water molecules. To understand the role of TSHR-associated water molecules we used molecular dynamic simulations of the TMD and identified a network of putative receptor associated transmembrane water channels. This result was confirmed with extended simulations of the full length TSHR with and without TSH ligand binding. While the transport time observed in the simulations via the TSHR protein was slower than via the lipid bi...
Source: Endocrinology - September 28, 2023 Category: Endocrinology Authors: Rauf Latif Terry F Davies Mihaly Mezei Source Type: research
