lnc RNA LOC102163816 Promotes Proliferation of Porcine Follicular Granulosa Cells Via miR-455-3p/PTK2B/PI3K/AKT Pathway
This study demonstrated that LOC102163816 is upregulated in porcine GCs treated with follicular fluid exosomes. Further study defined LOC102163816 to be a novel long noncoding RNA that is highly homologous to human metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and enriched in porcine follicular fluid exosomes. We have speculated that LOC102163816 might have a cell-proliferative effect similar to that of MALAT1. We found that overexpression of LOC102163816 promoted transition from the G1 phase to the S phase of the cell cycle, thereby promoting proliferation of GCs. To explore the specific mechanism underl...
Source: Endocrinology - January 30, 2024 Category: Endocrinology Authors: Caomeihui Shen Tong Chen Guitian He Kening Liu Maosheng Cao Chenfeng Yuan Boqi Zhang Xue Chen Lu Chen Yuxin Luo Yueying Wang Xu Zhou Chunjin Li Source Type: research
Profiling of unfolded protein response markers and effect of IRE1 α-specific inhibitor in pituitary neuroendocrine tumor
CONCLUSION: UPR markers in FPitNETs are implicated as dominant A-UPR but blunted T-UPR. KIRA8, enhanced with octreotide, unbalances UPR, leading to anti-tumor effects. Targeting IRE1α may provide a novel strategy to treat PitNETs.PMID:38289718 | DOI:10.1210/endocr/bqae008 (Source: Endocrinology)
Source: Endocrinology - January 30, 2024 Category: Endocrinology Authors: Shuhei Morita Shinsuke Uraki Hiroyuki Ariyasu Tomoya Tsuji Asako Doi Hiroto Furuta Toshikazu Yamoto Naoyuki Nakao Takashi Akamizu Taka-Aki Matsuoka Source Type: research
Maternal High-Fat Diet Affects the Contents of Eggs and Causes Abnormal Development in the Medaka Fish
Endocrinology. 2024 Jan 16;165(3):bqae006. doi: 10.1210/endocr/bqae006.ABSTRACTMaternal nutritional status can affect development and metabolic phenotypes of progeny in animals. The effects of maternal diet are thought to be mediated mainly by changes inside oocytes such as organelles, maternal RNAs, and metabolites. However, to what extent each factor contributes to offspring phenotypes remains uncertain, especially in viviparous mammalian systems, where factors other than oocytes, such as placenta and milk, need to be considered. Here, using the medaka fish as an oviparous vertebrate model, we examined whether maternal h...
Source: Endocrinology - January 27, 2024 Category: Endocrinology Authors: Yusuke Inoue Manatsu Fukushima Go Hirasawa Fumiya Furukawa Hiroyuki Takeda Chie Umatani Source Type: research
Long-term recordings of arcuate nucleus kisspeptin neurons across the mouse estrous cycle
Endocrinology. 2024 Jan 27:bqae009. doi: 10.1210/endocr/bqae009. Online ahead of print.ABSTRACTThe arcuate nucleus kisspeptin (ARNKISS) neurons represent the gonadotropin-releasing hormone (GnRH) pulse generator that likely drives pulsatile gonadotropin secretion in all mammals. Using an improved GCaMP fiber photometry system enabling long-term continuous recordings, we aimed here to establish a definitive profile of ARNKISS neuronal activity across the murine estrous cycle. As noted previously, a very substantial reduction in the frequency of ARNKISS neuron synchronization events (SEs) occurs on late proestrus and extends...
Source: Endocrinology - January 27, 2024 Category: Endocrinology Authors: Szilvia Vas Ellen Wall Ziyue Zhou Lajos Kalmar Su Young Han Allan E Herbison Source Type: research
FGF21 Induces Skeletal Muscle Atrophy and Increases Amino Acids in Female Mice: A Potential Role for Glucocorticoids
Endocrinology. 2024 Jan 16;165(3):bqae004. doi: 10.1210/endocr/bqae004.ABSTRACTFibroblast growth factor-21 (FGF21) is an intercellular signaling molecule secreted by metabolic organs, including skeletal muscle, in response to intracellular stress. FGF21 crosses the blood-brain barrier and acts via the nervous system to coordinate aspects of the adaptive starvation response, including increased lipolysis, gluconeogenesis, fatty acid oxidation, and activation of the hypothalamic-pituitary-adrenocortical (HPA) axis. Given its beneficial effects for hepatic lipid metabolism, pharmaceutical FGF21 analogues are used in clinical ...
Source: Endocrinology - January 20, 2024 Category: Endocrinology Authors: Karlton R Larson Devi Jayakrishnan Karla A Soto Sauza Michael L Goodson Aki T Chaffin Arik Davidyan Suraj Pathak Yanbin Fang Diego Gonzalez Maga ña Benjamin F Miller Karen K Ryan Source Type: research
Inhibition of CSF1R and KIT with pexidartinib reduces inflammatory signaling and cell viability in endometriosis
Endocrinology. 2024 Jan 16:bqae003. doi: 10.1210/endocr/bqae003. Online ahead of print.ABSTRACTEndometriosis is a common and debilitating disease, affecting ∼170 million women worldwide. Affected patients have limited therapeutic options such as hormonal suppression or surgical excision of the lesions, though therapies are often not completely curative. Targeting receptor tyrosine-kinases (RTKs) could provide a nonhormonal treatment option for endometriosis. We determined that two RTKs, Macrophage colony stimulating factor receptor (CSF1R) and Mast/stem cell growth factor receptor KIT (KIT), are overexpressed in endometr...
Source: Endocrinology - January 16, 2024 Category: Endocrinology Authors: Timothy N Dunn Dominique I Cope Suni Tang Tirupataiah Sirupangi Sydney E Parks Zian Liao Fei Yuan Chad J Creighton Ramya P Masand Linda Alpuing Radilla Xiaoming Guan Laura Detti Diana Monsivais Martin M Matzuk Source Type: research
Discordant Effects of Polyamine Depletion by DENSpm and DFMO on β-cell Cytokine Stress and Diabetes Outcomes in Mice
In this study, we show that DENSpm depletes intracellular polyamines as effectively as DFMO in mouse β cells. RNA-sequencing analysis, however, suggests that the cellular responses to DENSpm and DFMO differ, with both showing effects on cellular proliferation but the latter showing additional effects on mRNA translation and protein-folding pathways. In the low-dose streptozotocin-induced mouse model of T1D, DENSpm, unlike DFMO, did not prevent or delay diabetes outcomes but did result in improvements in glucose tolerance and reductions in islet oxidative stress. In nonobese diabetic (NOD) mice, short-term DENSpm administr...
Source: Endocrinology - January 9, 2024 Category: Endocrinology Authors: Batoul Hammoud Jennifer B Nelson Sarah C May Sarah A Tersey Raghavendra G Mirmira Source Type: research
Endometrial BMP2 Deficiency Impairs ITGB3-Mediated Trophoblast Invasion in Women With Repeated Implantation Failure
CONCLUSION: Our results demonstrate endometrial BMP2 deficiency in patients with RIF. ITGB3 mediates both basal and BMP2-promoted human trophoblast invasion and is essential for early placentation. These findings broaden our knowledge regarding the regulation of early placentation and provide candidate diagnostic and therapeutic targets for RIF clinical management.PMID:38195194 | DOI:10.1210/endocr/bqae002 (Source: Endocrinology)
Source: Endocrinology - January 9, 2024 Category: Endocrinology Authors: Cuiping Hu Jianye Deng Mingxi Liu Tianxiang Ni Zi-Jiang Chen Junhao Yan Yan Li Source Type: research
Granulosa Cells Alone, Without Theca Cells, Can Mediate LH-induced Oocyte Meiotic Resumption
Endocrinology. 2024 Jan 16;165(3):bqad200. doi: 10.1210/endocr/bqad200.ABSTRACTSignaling in the granulosa cells of mammalian ovarian follicles is necessary for maintaining prophase arrest in the oocyte and for mediating the resumption of meiosis in response to luteinizing hormone (LH). However, the follicle also includes an outer layer of theca cells, some of which express receptors for LH. To investigate whether theca cells are required for maintaining meiotic arrest and reinitiating meiosis in response to LH, we mechanically separated the granulosa cells and oocyte from the theca and basal lamina. This was accomplished b...
Source: Endocrinology - January 5, 2024 Category: Endocrinology Authors: Rachael P Norris Laurinda A Jaffe Source Type: research
Granulosa cells alone, without theca cells, can mediate LH-induced oocyte meiotic resumption
Endocrinology. 2024 Jan 5:bqad200. doi: 10.1210/endocr/bqad200. Online ahead of print.ABSTRACTSignaling in the granulosa cells of mammalian ovarian follicles is necessary for maintaining prophase arrest in the oocyte and for mediating the resumption of meiosis in response to luteinizing hormone (LH). However, the follicle also includes an outer layer of theca cells, some of which express receptors for LH. To investigate whether the theca cells are required for maintaining meiotic arrest and reinitiating meiosis in response to LH, we mechanically separated the granulosa cells and oocyte from the theca and basal lamina. This...
Source: Endocrinology - January 5, 2024 Category: Endocrinology Authors: Rachael P Norris Laurinda A Jaffe Source Type: research
Granulosa cells alone, without theca cells, can mediate LH-induced oocyte meiotic resumption
Endocrinology. 2024 Jan 5:bqad200. doi: 10.1210/endocr/bqad200. Online ahead of print.ABSTRACTSignaling in the granulosa cells of mammalian ovarian follicles is necessary for maintaining prophase arrest in the oocyte and for mediating the resumption of meiosis in response to luteinizing hormone (LH). However, the follicle also includes an outer layer of theca cells, some of which express receptors for LH. To investigate whether the theca cells are required for maintaining meiotic arrest and reinitiating meiosis in response to LH, we mechanically separated the granulosa cells and oocyte from the theca and basal lamina. This...
Source: Endocrinology - January 5, 2024 Category: Endocrinology Authors: Rachael P Norris Laurinda A Jaffe Source Type: research
Granulosa cells alone, without theca cells, can mediate LH-induced oocyte meiotic resumption
Endocrinology. 2024 Jan 5:bqad200. doi: 10.1210/endocr/bqad200. Online ahead of print.ABSTRACTSignaling in the granulosa cells of mammalian ovarian follicles is necessary for maintaining prophase arrest in the oocyte and for mediating the resumption of meiosis in response to luteinizing hormone (LH). However, the follicle also includes an outer layer of theca cells, some of which express receptors for LH. To investigate whether the theca cells are required for maintaining meiotic arrest and reinitiating meiosis in response to LH, we mechanically separated the granulosa cells and oocyte from the theca and basal lamina. This...
Source: Endocrinology - January 5, 2024 Category: Endocrinology Authors: Rachael P Norris Laurinda A Jaffe Source Type: research
KNDy Neurons of the Hypothalamus and Their Role in GnRH Pulse Generation: an Update
Endocrinology. 2023 Dec 23;165(2):bqad194. doi: 10.1210/endocr/bqad194.ABSTRACTThere is considerable evidence that synchronized activity within a reciprocally connected population of cells in the arcuate nucleus (ARC) coexpressing kisspeptin, neurokinin B (NKB), and dynorphin (KNDy cells) is crucial for the generation of gonadotrophin-releasing hormone (GnRH) pulses in mammals. The initial "KNDy hypothesis" proposed that pulsatile GnRH secretion is elicited by episodic kisspeptin release from KNDy cells following synchronized activation and termination of the population by NKB and dynorphin, respectively. Since then, the r...
Source: Endocrinology - January 3, 2024 Category: Endocrinology Authors: Aleisha M Moore Alyssa G Novak Michael N Lehman Source Type: research
KNDy neurons of the hypothalamus and their role in GnRH pulse generation: an update
Endocrinology. 2024 Jan 3:bqad194. doi: 10.1210/endocr/bqad194. Online ahead of print.ABSTRACTThere is considerable evidence that synchronized activity within a reciprocally connected population of cells in the arcuate nucleus (ARC) co-expressing kisspeptin, neurokinin B (NKB) and dynorphin (KNDy cells) is crucial for the generation of gonadotrophin-releasing hormone (GnRH) pulses in mammals. The initial "KNDy hypothesis" proposed that pulsatile GnRH secretion is elicited by episodic kisspeptin release from KNDy cells following synchronized activation and termination of the population by NKB and dynorphin, respectively. Si...
Source: Endocrinology - January 3, 2024 Category: Endocrinology Authors: Aleisha M Moore Alyssa G Novak Michael N Lehman Source Type: research
Reproductive Profile of Neuronal Androgen Receptor Knockout Female Mice with Low Dose of DHT
Endocrinology. 2023 Dec 29:bqad199. doi: 10.1210/endocr/bqad199. Online ahead of print.ABSTRACTHyperandrogenism and polycystic ovarian syndrome (PCOS) result from the imbalance or increase of androgen levels in females. Androgen Receptor (AR) mediates the effects of androgens, and this study examines whether neuronal AR plays a role in reproduction under normal and increased androgen conditions in female mice. The neuron specific AR knockout mouse (SynARKO) was generated from a female mouse (synapsin promoter driven Cre) and a male mouse (Ar fl/y). Puberty onset and the levels of reproductive hormones such as LH, FSH, test...
Source: Endocrinology - December 29, 2023 Category: Endocrinology Authors: Vaibhave Ubba Serene Joseph Olubusayo Awe Dustin Jones Milan K Dsilva Mingxiao Feng Junjiang Wang Xiaomin Fu Razeen J Akbar Brittany H Bodnar Wenhui Hu Hong Wang Xiaofeng Yang Ling Yang Peixin Yang Bouchra Taib Rexford Ahima Sara Divall Sheng Wu Source Type: research
