Silencing KPNA2 Promotes Ferroptosis in Laryngeal Cancer by Activating the FoxO Signaling Pathway
Biochem Genet. 2024 Feb 20. doi: 10.1007/s10528-023-10655-8. Online ahead of print.ABSTRACTWe aim to clarify the specific role of Karyopherin α2 (KPNA2) in the progression of laryngeal cancer, a kind of malignant tumor with a poor curative effect. We performed the bioinformatic analysis to obtain the ferroptosis-related differentially expressed genes. KPNA2 was screened out. Then the CCK-8 assay, wound healing assay, and transwell assay were used to clarify the changes in the proliferation, migration, and invasion abilities of laryngeal cancer cells after silencing KPNA2. The concentrations of iron ions, glutathione, supe...
Source: Biochemical Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Mimi Xu Xiaoqi Hu Zhixue Xiao Siyi Zhang Zhongming Lu Source Type: research
Propofol Ameliorates Spinal Cord Injury Process by Mediating miR-672-3p/TNIP2 Axis
In conclusion, propofol increased TNIP2 level by reducing miR-672-3p expression, thereby alleviating LPS-induced PC12 cell injury and improving the motor function of SCI rat models.PMID:38379038 | DOI:10.1007/s10528-024-10718-4 (Source: Biochemical Genetics)
Source: Biochemical Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Chengliang Sun Dongzhi Liu Shunheng Gao Mingyu Xiu Zhaojian Zhang Source Type: research
Daturataturin A Ameliorates Psoriasis by Regulating PPAR Pathway
The objective of this study is to elucidate the effect of DTA on psoriasis and its potential mechanism. We established psoriasis-like keratinocytes model by stimulating HaCaT cells with M5 cocktail cytokines including Interleukin (IL)-17A, IL-22, oncostatin M, IL-1α, and tumor necrosis factor-α (TNF-α), followed by intervention with DTA. The potential effects and mechanisms of DTA on psoriasis were evaluated in vitro. DTA was found to be able to inhibit hyperproliferation, promote apoptosis, decrease the release of pro-inflammatory cytokines, downregulate keratin expression, and improve lipid metabolism via regulating t...
Source: Biochemical Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Zheng Wei Hongfa Zhong Shanmin Yuan Cong Chen Source Type: research
Long Non-coding RNA 02298 Promotes the Malignancy of HCC by Targeting the miR-28-5p/CCDC6 Pathway
Biochem Genet. 2024 Feb 21. doi: 10.1007/s10528-023-10662-9. Online ahead of print.ABSTRACTHepatocellular carcinoma (HCC) is a malignancy characterized by a high fatality rate. Increasing evidence indicating that long non-coding RNAs (lncRNAs) play a regulatory role in hepatocellular carcinoma (HCC). Among them, the correlation between LINC02298 and HCC remains unknown. The expression and subcellular localization of LINC02298 in HCC tissues and cell lines were detected by real-time quantitative polymerase chain reaction (RT-qPCR). Furthermore, the correlation between the expression of LINC02298 and clinicopathological feat...
Source: Biochemical Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Jinyi Wang Bin Xu Litao Liang Qi Chen Source Type: research
Albiflorin Alleviates Severe Acute Pancreatitis-Associated Liver Injury by Inactivating P38MAPK/NF- κB Signaling Pathway
Biochem Genet. 2024 Feb 21. doi: 10.1007/s10528-024-10686-9. Online ahead of print.ABSTRACTAlbiflorin (Alb) is a monoterpenoid component that is commonly found in Paeonia lactiflora Pall. or Paeonia veitchii Lynch. It is known for its impressive anti-oxidant and anti-inflammatory properties. However, the effect of Alb on severe acute pancreatitis (SAP)-associated liver injury has not been fully understood. To investigate this, we conducted a study using a rat model of SAP induced by administering two intraperitoneal injections of 20% L-arginine (3.3 g/kg) over a period of 2 h. Subsequently, the SAP-induced rats were random...
Source: Biochemical Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Haitao Li Xiangpeng Zeng Dongjie Sun Xingfeng Qi Dazhou Li Wen Wang Yan Lin Source Type: research
The Role of miR-29a and miR-143 on the Anti-apoptotic MCL-1/cIAP-2 Genes Expression in EGFR Mutated Non-small Cell Lung Carcinoma Patients
Biochem Genet. 2024 Feb 20. doi: 10.1007/s10528-024-10740-6. Online ahead of print.ABSTRACTThe survival rate of lung cancer is low due to the high frequency of drug resistance in patients with mutations in the driver genes. Overexpression of anti-apoptotic genes is one of the most prominent features of tumor drug resistance. EGFR signaling induces the expression of anti-apoptotic genes. Also, microRNAs (miRNAs) have a critical role in regulating biological functions such as apoptosis; a process mostly eluded in cancer progression. The mutation screening was performed on one thousand non-small cell lung carcinoma patients t...
Source: Biochemical Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Zahra Abrehdari-Tafreshi Ehsan Arefian Nasser Rakhshani S Mahmoud A Najafi Source Type: research
Silencing KPNA2 Promotes Ferroptosis in Laryngeal Cancer by Activating the FoxO Signaling Pathway
Biochem Genet. 2024 Feb 20. doi: 10.1007/s10528-023-10655-8. Online ahead of print.ABSTRACTWe aim to clarify the specific role of Karyopherin α2 (KPNA2) in the progression of laryngeal cancer, a kind of malignant tumor with a poor curative effect. We performed the bioinformatic analysis to obtain the ferroptosis-related differentially expressed genes. KPNA2 was screened out. Then the CCK-8 assay, wound healing assay, and transwell assay were used to clarify the changes in the proliferation, migration, and invasion abilities of laryngeal cancer cells after silencing KPNA2. The concentrations of iron ions, glutathione, supe...
Source: Biochemical Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Mimi Xu Xiaoqi Hu Zhixue Xiao Siyi Zhang Zhongming Lu Source Type: research
Propofol Ameliorates Spinal Cord Injury Process by Mediating miR-672-3p/TNIP2 Axis
In conclusion, propofol increased TNIP2 level by reducing miR-672-3p expression, thereby alleviating LPS-induced PC12 cell injury and improving the motor function of SCI rat models.PMID:38379038 | DOI:10.1007/s10528-024-10718-4 (Source: Biochemical Genetics)
Source: Biochemical Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Chengliang Sun Dongzhi Liu Shunheng Gao Mingyu Xiu Zhaojian Zhang Source Type: research
Daturataturin A Ameliorates Psoriasis by Regulating PPAR Pathway
The objective of this study is to elucidate the effect of DTA on psoriasis and its potential mechanism. We established psoriasis-like keratinocytes model by stimulating HaCaT cells with M5 cocktail cytokines including Interleukin (IL)-17A, IL-22, oncostatin M, IL-1α, and tumor necrosis factor-α (TNF-α), followed by intervention with DTA. The potential effects and mechanisms of DTA on psoriasis were evaluated in vitro. DTA was found to be able to inhibit hyperproliferation, promote apoptosis, decrease the release of pro-inflammatory cytokines, downregulate keratin expression, and improve lipid metabolism via regulating t...
Source: Biochemical Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Zheng Wei Hongfa Zhong Shanmin Yuan Cong Chen Source Type: research
Long Non-coding RNA 02298 Promotes the Malignancy of HCC by Targeting the miR-28-5p/CCDC6 Pathway
Biochem Genet. 2024 Feb 21. doi: 10.1007/s10528-023-10662-9. Online ahead of print.ABSTRACTHepatocellular carcinoma (HCC) is a malignancy characterized by a high fatality rate. Increasing evidence indicating that long non-coding RNAs (lncRNAs) play a regulatory role in hepatocellular carcinoma (HCC). Among them, the correlation between LINC02298 and HCC remains unknown. The expression and subcellular localization of LINC02298 in HCC tissues and cell lines were detected by real-time quantitative polymerase chain reaction (RT-qPCR). Furthermore, the correlation between the expression of LINC02298 and clinicopathological feat...
Source: Biochemical Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Jinyi Wang Bin Xu Litao Liang Qi Chen Source Type: research
Albiflorin Alleviates Severe Acute Pancreatitis-Associated Liver Injury by Inactivating P38MAPK/NF- κB Signaling Pathway
Biochem Genet. 2024 Feb 21. doi: 10.1007/s10528-024-10686-9. Online ahead of print.ABSTRACTAlbiflorin (Alb) is a monoterpenoid component that is commonly found in Paeonia lactiflora Pall. or Paeonia veitchii Lynch. It is known for its impressive anti-oxidant and anti-inflammatory properties. However, the effect of Alb on severe acute pancreatitis (SAP)-associated liver injury has not been fully understood. To investigate this, we conducted a study using a rat model of SAP induced by administering two intraperitoneal injections of 20% L-arginine (3.3 g/kg) over a period of 2 h. Subsequently, the SAP-induced rats were random...
Source: Biochemical Genetics - February 21, 2024 Category: Genetics & Stem Cells Authors: Haitao Li Xiangpeng Zeng Dongjie Sun Xingfeng Qi Dazhou Li Wen Wang Yan Lin Source Type: research
Genes and Pathways Underpinning Klinefelter Syndrome at Bulk and Single-Cell Levels
Biochem Genet. 2024 Feb 19. doi: 10.1007/s10528-024-10689-6. Online ahead of print.ABSTRACTKlinefelter syndrome (KS) is the most frequent genetic anomaly in infertile men. Given its unclear mechanism, we aim to investigate critical genes and pathways in the pathogenesis of KS based on three bulk and one single-cell transcriptome data sets from Gene Expression Omnibus. We merged two data sets (GSE42331 and GSE47584) with human KS whole blood samples. When comparing the control and KS samples, five hub genes, including defensin alpha 4 (DEFA4), bactericidal permeability increasing protein (BPI), myeloperoxidase (MPO), intele...
Source: Biochemical Genetics - February 20, 2024 Category: Genetics & Stem Cells Authors: Linlin Tian Yan Yu Ziqing Mao Dandan Xu Hongbo Zhang Mengkai Qiao Tong Chen Wen Liu Source Type: research
Circ-RNF111 Promotes Proliferation of Ovarian Cancer Cell SKOV-3 by Targeting the MiR-556-5p/CCND1 Axis
Biochem Genet. 2024 Feb 20. doi: 10.1007/s10528-024-10665-0. Online ahead of print.ABSTRACTThe aim of this study was to investigate the role and mechanism of circ-RNF111 in the human ovarian cancer cell line SKOV-3. First, qRT-PCR was used to detect circ-RNF111 and miR-556-5p expression levels in human normal ovarian epithelial cells IOSE80 and human ovarian cancer cells SKOV-3. CCK-8 and colony formation assays were adopted to determine the proliferation rate and cell viability of SKOV-3 cells, respectively. Additionally, in an attempt to reveal the mechanism of circ-RNF111, we predicted the targeting relationship between...
Source: Biochemical Genetics - February 20, 2024 Category: Genetics & Stem Cells Authors: Li Zuo Yue Tan Qiao-Ling Xu Xiao-Li Li Mi Xiao Source Type: research
Circ-Luc7l Absence Attenuates Diabetic Nephropathy Progression by Reducing Mesangial Cell Excessive Proliferation, Inflammation, and Extracellular Matrix Accumulation via Mediating the miR-205-5p/Tgfbr1 Pathway
Biochem Genet. 2024 Feb 20. doi: 10.1007/s10528-024-10694-9. Online ahead of print.ABSTRACTDiabetic nephropathy (DN) threatens the survival quality of patients, with complex pathogenesis. Circular RNA (circRNA) dysregulation occurs in DN development. This work aimed to investigate the role of circ-Luc7l in DN cell models and related molecular mechanisms. The expression of circ-Luc7l, microRNA (miR)-205-5p, and transforming growth factor-beta receptor 1 (Tgfbr1) was examined by real-time quantitative PCR (RT-qPCR). Cell viability and proliferation were detected by Cell Counting Kit-8 (CCK-8) assay and EdU assay. The express...
Source: Biochemical Genetics - February 20, 2024 Category: Genetics & Stem Cells Authors: Zhan Fang Dan Wang Fang Sun Jing Chang Dong Yuan Shuhua Lin Jian Teng Source Type: research
Effects of KLF11 on Vascular Smooth Muscle Cells and its Underlying Mechanisms in Intracranial Aneurysm
This study aimed to analyze the effects of KLF11 on H2O2-induced human brain VSMCs (HBVSMCs) in IA. We determined the mRNA levels of KLF11 in 15 paired arterial wall tissues of patients with IA and healthy volunteers. HBVSMCs were stimulated with H2O2 for 6 h to establish an IA model in vitro. Cell viability, apoptosis, and inflammatory cytokine (interleukin [IL-1β, tumor necrosis factor-α, and IL-6) levels were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide, flow cytometry, and enzyme-linked immunosorbent assays, respectively. KLF11 expression was determined via quantitative reverse tran...
Source: Biochemical Genetics - February 18, 2024 Category: Genetics & Stem Cells Authors: Ke Yan Jiarong Bian Liang He Bingwei Song Linhai Shen Yong Zhen Source Type: research
