Circ-Luc7l Absence Attenuates Diabetic Nephropathy Progression by Reducing Mesangial Cell Excessive Proliferation, Inflammation, and Extracellular Matrix Accumulation via Mediating the miR-205-5p/Tgfbr1 Pathway
Biochem Genet. 2024 Feb 20. doi: 10.1007/s10528-024-10694-9. Online ahead of print.ABSTRACTDiabetic nephropathy (DN) threatens the survival quality of patients, with complex pathogenesis. Circular RNA (circRNA) dysregulation occurs in DN development. This work aimed to investigate the role of circ-Luc7l in DN cell models and related molecular mechanisms. The expression of circ-Luc7l, microRNA (miR)-205-5p, and transforming growth factor-beta receptor 1 (Tgfbr1) was examined by real-time quantitative PCR (RT-qPCR). Cell viability and proliferation were detected by Cell Counting Kit-8 (CCK-8) assay and EdU assay. The express...
Source: Biochemical Genetics - February 20, 2024 Category: Genetics & Stem Cells Authors: Zhan Fang Dan Wang Fang Sun Jing Chang Dong Yuan Shuhua Lin Jian Teng Source Type: research
Effects of KLF11 on Vascular Smooth Muscle Cells and its Underlying Mechanisms in Intracranial Aneurysm
This study aimed to analyze the effects of KLF11 on H2O2-induced human brain VSMCs (HBVSMCs) in IA. We determined the mRNA levels of KLF11 in 15 paired arterial wall tissues of patients with IA and healthy volunteers. HBVSMCs were stimulated with H2O2 for 6 h to establish an IA model in vitro. Cell viability, apoptosis, and inflammatory cytokine (interleukin [IL-1β, tumor necrosis factor-α, and IL-6) levels were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide, flow cytometry, and enzyme-linked immunosorbent assays, respectively. KLF11 expression was determined via quantitative reverse tran...
Source: Biochemical Genetics - February 18, 2024 Category: Genetics & Stem Cells Authors: Ke Yan Jiarong Bian Liang He Bingwei Song Linhai Shen Yong Zhen Source Type: research
Effects of KLF11 on Vascular Smooth Muscle Cells and its Underlying Mechanisms in Intracranial Aneurysm
This study aimed to analyze the effects of KLF11 on H2O2-induced human brain VSMCs (HBVSMCs) in IA. We determined the mRNA levels of KLF11 in 15 paired arterial wall tissues of patients with IA and healthy volunteers. HBVSMCs were stimulated with H2O2 for 6 h to establish an IA model in vitro. Cell viability, apoptosis, and inflammatory cytokine (interleukin [IL-1β, tumor necrosis factor-α, and IL-6) levels were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide, flow cytometry, and enzyme-linked immunosorbent assays, respectively. KLF11 expression was determined via quantitative reverse tran...
Source: Biochemical Genetics - February 18, 2024 Category: Genetics & Stem Cells Authors: Ke Yan Jiarong Bian Liang He Bingwei Song Linhai Shen Yong Zhen Source Type: research
Effects of KLF11 on Vascular Smooth Muscle Cells and its Underlying Mechanisms in Intracranial Aneurysm
This study aimed to analyze the effects of KLF11 on H2O2-induced human brain VSMCs (HBVSMCs) in IA. We determined the mRNA levels of KLF11 in 15 paired arterial wall tissues of patients with IA and healthy volunteers. HBVSMCs were stimulated with H2O2 for 6 h to establish an IA model in vitro. Cell viability, apoptosis, and inflammatory cytokine (interleukin [IL-1β, tumor necrosis factor-α, and IL-6) levels were assessed using the 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide, flow cytometry, and enzyme-linked immunosorbent assays, respectively. KLF11 expression was determined via quantitative reverse tran...
Source: Biochemical Genetics - February 18, 2024 Category: Genetics & Stem Cells Authors: Ke Yan Jiarong Bian Liang He Bingwei Song Linhai Shen Yong Zhen Source Type: research
Dynamic Expressions of Yellow Stripe-Like (YSL) Genes During Pod Development Shed Light on Associations with Iron Distribution in Phaseolus vulgaris
This study provides valuable insights into the gene regulatory basis of iron accumulation in bean pods and seeds.PMID:38367128 | DOI:10.1007/s10528-023-10658-5 (Source: Biochemical Genetics)
Source: Biochemical Genetics - February 17, 2024 Category: Genetics & Stem Cells Authors: Yihan Zhang Yunxiu Liu Hailan Li Ting Sun Min Xu Pei Xu Source Type: research
Uncovering the ceRNA Network Related to the Prognosis of Stomach Adenocarcinoma Among 898 Patient Samples
In this study, we aimed to elucidate the roles of lncRNAs in the ceRNA network of STAD, uncovering the molecular biomarkers for target therapy and prognosis. Specifically, a multitude of differentially expressed lncRNAs, miRNAs, and mRNAs (i.e., 898 samples in total) was collected and processed from TCGA. Cytoplasmic lncRNAs were kept for evaluating overall survival (OS) time and constructing the ceRNA network. Differentially expressed mRNAs in the ceRNA network were also investigated for functional and pathological insights. Interestingly, we identified one ceRNA network including 13 lncRNAs, 25 miRNAs, and 9 mRNAs. Among...
Source: Biochemical Genetics - February 16, 2024 Category: Genetics & Stem Cells Authors: Zhe Liu Fang Liu Olutomilayo Olayemi Petinrin Fuzhou Wang Yu Zhang Ka-Chun Wong Source Type: research
Occurrence of Existing BCR-ABL Baseline Mutations and Associated Haplotype (NmR) Among CML Patients with Diverse IM Response: A Hospital-based Study from North-East India
This study was conducted to investigate the potential role of three significant SNPs in CML progression due to IM resistance. During the study period from 2018 to 2022 (48 months), the blood samples from 219 Reverse transcriptase-PCR-confirmed CML patients following RNA extraction and cDNA preparation were subjected to M351T, E255K, and Y253H mutation analysis by PCR-RFLP. After agarose gel visualization, the samples were subjected to Sanger sequencing to confirm the nucleotide change at the polymorphic loci. The wild-type genotype of all three ABL1 SNPs under investigation exhibits a significant reduction in frequency amo...
Source: Biochemical Genetics - February 16, 2024 Category: Genetics & Stem Cells Authors: Gautam Hazarika Manash Jyoti Kalita Partha Pratim Das Simanta Kalita Kalpajit Dutta Lipika Lahkar Anjanjyoti Rajkonwar Mohammed Ghaznavi Idris Vinotsale Khamo Giriraj Kusre Subhash Medhi Source Type: research
Uncovering the ceRNA Network Related to the Prognosis of Stomach Adenocarcinoma Among 898 Patient Samples
In this study, we aimed to elucidate the roles of lncRNAs in the ceRNA network of STAD, uncovering the molecular biomarkers for target therapy and prognosis. Specifically, a multitude of differentially expressed lncRNAs, miRNAs, and mRNAs (i.e., 898 samples in total) was collected and processed from TCGA. Cytoplasmic lncRNAs were kept for evaluating overall survival (OS) time and constructing the ceRNA network. Differentially expressed mRNAs in the ceRNA network were also investigated for functional and pathological insights. Interestingly, we identified one ceRNA network including 13 lncRNAs, 25 miRNAs, and 9 mRNAs. Among...
Source: Biochemical Genetics - February 16, 2024 Category: Genetics & Stem Cells Authors: Zhe Liu Fang Liu Olutomilayo Olayemi Petinrin Fuzhou Wang Yu Zhang Ka-Chun Wong Source Type: research
Occurrence of Existing BCR-ABL Baseline Mutations and Associated Haplotype (NmR) Among CML Patients with Diverse IM Response: A Hospital-based Study from North-East India
This study was conducted to investigate the potential role of three significant SNPs in CML progression due to IM resistance. During the study period from 2018 to 2022 (48 months), the blood samples from 219 Reverse transcriptase-PCR-confirmed CML patients following RNA extraction and cDNA preparation were subjected to M351T, E255K, and Y253H mutation analysis by PCR-RFLP. After agarose gel visualization, the samples were subjected to Sanger sequencing to confirm the nucleotide change at the polymorphic loci. The wild-type genotype of all three ABL1 SNPs under investigation exhibits a significant reduction in frequency amo...
Source: Biochemical Genetics - February 16, 2024 Category: Genetics & Stem Cells Authors: Gautam Hazarika Manash Jyoti Kalita Partha Pratim Das Simanta Kalita Kalpajit Dutta Lipika Lahkar Anjanjyoti Rajkonwar Mohammed Ghaznavi Idris Vinotsale Khamo Giriraj Kusre Subhash Medhi Source Type: research
Global DNA Methylation Levels Viz-a-Viz Genetic and Biochemical Variations in One Carbon Metabolic Pathway: An Exploratory Study from North India
This study aimed to understand the variations in global DNA methylation levels with respect to selected genetic (CBS 844ins68, MTRR A66G, MTR A2756G, and MTHFR C677T polymorphisms) and biochemical (folate, vitamin B12, and homocysteine) markers associated with OCMP among healthy North Indian adults. The study has been conducted among 1095 individuals of either sex (69.5% females), aged 30-75 years. A sample of 5 mL of blood was collected from each participant. Homocysteine, folate, and vitamin B12 levels were determined using the chemiluminescence technique. Restriction digestion was performed for genotyping MTRR A66G, MTR...
Source: Biochemical Genetics - February 15, 2024 Category: Genetics & Stem Cells Authors: Vineet Chaudhary Debashis Bhattacharjee Naorem Kiranmala Devi Kallur Nava Saraswathy Source Type: research
CircRNA ITCH Inhibits Epithelial-Mesenchymal Transformation and Promotes Apoptosis in Papillary Thyroid Carcinoma via miR-106a-5p/JAZF1 Axis
This study uncovered that circ-ITCH suppressed the development of PTC cells at least partly by mediating miR-106a-5p/JAZF1 network.PMID:38358587 | DOI:10.1007/s10528-024-10672-1 (Source: Biochemical Genetics)
Source: Biochemical Genetics - February 15, 2024 Category: Genetics & Stem Cells Authors: Yijun Chen Zhiming Lian Guolie Zhang Yuanmei Lin Guoliang Zhang Wei Liu Jian Gao Zifang Zheng Source Type: research
Exploring SoySNP50K and USDA Germplasm Collection Data to Find New QTLs Associated with Protein and Oil Content in Brazilian Genotypes
This study utilized publicly available data to investigate SNP markers associated with protein and oil content in Brazilian soybeans. Through this research, twenty-two new QTLs (Quantitative Trait Loci) were identified, and we highlighted the substantial influence of Roanoke, Lee and Bragg ancestor on the genetic makeup of Brazilian soybean varieties. Our findings demonstrate that certain markers are being lost in modern cultivars, while others maintain or even increase their frequency. These observations indicate genomic regions that have undergone selection during soybean introduction in Brazil and could be valuable in b...
Source: Biochemical Genetics - February 15, 2024 Category: Genetics & Stem Cells Authors: Jessica Nayara Bas ílio Silva Rafael Delmond Bueno Teresinha de Jesus Feitosa de Sousa Yan Pablo Moreira Xavier Luiz Claudio Costa Silva Newton Deniz Piovesan Cleberson Ribeiro Maximiller Dal-Bianco Source Type: research
Global DNA Methylation Levels Viz-a-Viz Genetic and Biochemical Variations in One Carbon Metabolic Pathway: An Exploratory Study from North India
This study aimed to understand the variations in global DNA methylation levels with respect to selected genetic (CBS 844ins68, MTRR A66G, MTR A2756G, and MTHFR C677T polymorphisms) and biochemical (folate, vitamin B12, and homocysteine) markers associated with OCMP among healthy North Indian adults. The study has been conducted among 1095 individuals of either sex (69.5% females), aged 30-75 years. A sample of 5 mL of blood was collected from each participant. Homocysteine, folate, and vitamin B12 levels were determined using the chemiluminescence technique. Restriction digestion was performed for genotyping MTRR A66G, MTR...
Source: Biochemical Genetics - February 15, 2024 Category: Genetics & Stem Cells Authors: Vineet Chaudhary Debashis Bhattacharjee Naorem Kiranmala Devi Kallur Nava Saraswathy Source Type: research
CircRNA ITCH Inhibits Epithelial-Mesenchymal Transformation and Promotes Apoptosis in Papillary Thyroid Carcinoma via miR-106a-5p/JAZF1 Axis
This study uncovered that circ-ITCH suppressed the development of PTC cells at least partly by mediating miR-106a-5p/JAZF1 network.PMID:38358587 | DOI:10.1007/s10528-024-10672-1 (Source: Biochemical Genetics)
Source: Biochemical Genetics - February 15, 2024 Category: Genetics & Stem Cells Authors: Yijun Chen Zhiming Lian Guolie Zhang Yuanmei Lin Guoliang Zhang Wei Liu Jian Gao Zifang Zheng Source Type: research
Exploring SoySNP50K and USDA Germplasm Collection Data to Find New QTLs Associated with Protein and Oil Content in Brazilian Genotypes
This study utilized publicly available data to investigate SNP markers associated with protein and oil content in Brazilian soybeans. Through this research, twenty-two new QTLs (Quantitative Trait Loci) were identified, and we highlighted the substantial influence of Roanoke, Lee and Bragg ancestor on the genetic makeup of Brazilian soybean varieties. Our findings demonstrate that certain markers are being lost in modern cultivars, while others maintain or even increase their frequency. These observations indicate genomic regions that have undergone selection during soybean introduction in Brazil and could be valuable in b...
Source: Biochemical Genetics - February 15, 2024 Category: Genetics & Stem Cells Authors: Jessica Nayara Bas ílio Silva Rafael Delmond Bueno Teresinha de Jesus Feitosa de Sousa Yan Pablo Moreira Xavier Luiz Claudio Costa Silva Newton Deniz Piovesan Cleberson Ribeiro Maximiller Dal-Bianco Source Type: research
