circRNA-CPA4 Regulates Cell Proliferation and Apoptosis of Non-small Cell Lung Cancer via the miR-1183/PDPK1 Axis
This study elucidates the role of circRNA-CPA4 in NSCLC. Elevated circRNA-CPA4 expression in NSCLC lines was confirmed through qRT-PCR. Silencing circRNA-CPA4 with shRNA revealed, through CCK-8, colony formation, and flow cytometry assays, a notable constraint on proliferation and promotion of apoptosis in NSCLC cells. Subcellular localization analysis, RNA immunoprecipitation, and expression level assessments were employed to decipher the intricate interplay among miR-1183, circRNA-CPA4, and PDPK1. Results demonstrated heightened circRNA-CPA4 levels in NSCLC, and its knockdown curtailed NSCLC growth in vivo. Acting as a m...
Source: Biochemical Genetics - January 25, 2024 Category: Genetics & Stem Cells Authors: Heng Li Yujie Lei Nan Chen Gang Guo Xudong Xiang Yunchao Huang Source Type: research
Molecular Network Mechanism Analysis of Urine Stem Cells Against Retinal Aging
Biochem Genet. 2024 Jan 25. doi: 10.1007/s10528-023-10487-6. Online ahead of print.ABSTRACTTo investigate the effect and potential mechanism of human-derived urine stem cells (hUSCs) in inhibiting retinal aging by using experimental and bioinformatics. Retinal pigment epithelial cells cultured in vitro, which were randomly divided into normal group, aging group and supernatant of hUSCs group. Cell counting kit-8 detection, senescence-related β-galactosidase, and Annexin V/PI staining were performed to detect cell viability, senescence, and apoptosis. Subsequently, bioinformatics methods were used to explore the underlying...
Source: Biochemical Genetics - January 25, 2024 Category: Genetics & Stem Cells Authors: Ning Bi Na Li Hua Liu Ting-Hua Wang Source Type: research
Identification ATP5F1D as a Biomarker Linked to Diagnosis, Prognosis, and Immune Infiltration in Endometrial Cancer Based on Data-Independent Acquisition (DIA) Analysis
Biochem Genet. 2024 Jan 24. doi: 10.1007/s10528-023-10646-9. Online ahead of print.ABSTRACTIn developed countries, endometrial cancer (EC) is the most prevalent gynecological cancer. ATP5F1D is a subunit of ATP synthase, as well as an important component of the mitochondrial electron transport chain (ETC). ETC plays a compelling role in carcinogenesis. To date, little is known about the role of ATP5F1D in EC. We undertook data-independent acquisition mass spectrometry (DIA-MS) of 20 EC patients, comprising 10 high-grade and 10 low-grade cancer tissues. Biological functions of differentially expressed genes (DEGs) were anal...
Source: Biochemical Genetics - January 24, 2024 Category: Genetics & Stem Cells Authors: Yuemei Cheng Xiaolei Liang Xuehan Bi Chang Liu Yongxiu Yang Source Type: research
Impact of Dysregulated LINC01559 and LINC01410 Expression on the Diagnosis and Survival of Non-Small Cell Lung Cancer
Biochem Genet. 2024 Jan 24. doi: 10.1007/s10528-023-10632-1. Online ahead of print.ABSTRACTLung cancer is a widely recognized cancer with a very low survival rate, as it is mostly diagnosed at advanced stages. The most prevalent type of lung cancer is non-small cell lung cancer (NSCLC). LncRNAs are widely involved in cancer progression and migration. Therefore, we intended to estimate the circulatory expression levels of LINC01559 and LINC01410 in NSCLC and their roles in tumor prognosis evaluation as less invasive potential markers. The relative expression levels of the plasma cell-free lncRNAs LINC01559 and LINC01410 in ...
Source: Biochemical Genetics - January 24, 2024 Category: Genetics & Stem Cells Authors: Amany A Saleh Yasser A Elghobashy Zeinab A Kasemy Amira Hegazy Abeer A ALrefai Source Type: research
The miR-9-5p/KLF5/IL-1 β Axis Regulates Airway Smooth Muscle Cell Proliferation and Apoptosis to Aggravate Airway Remodeling and Inflammation in Asthma
Biochem Genet. 2024 Jan 24. doi: 10.1007/s10528-023-10640-1. Online ahead of print.ABSTRACTThe aim of this study was to investigate the underlying mechanism of miR-9-5p in airway smooth muscle cells (ASMCs) of asthmatic mice. An asthmatic mouse model was established through the intraperitoneal injection of ovalbumin. Histopathological changes in lung tissues of asthmatic mice were observed using HE staining. ASMCs was identified using immunofluorescence staining and cell morphology. The mRNA expressions of miR-9-5p, KLF5, and IL-1β were measured using RT-qPCR. Additionally, CCK8 assay and flow cytometry were applied for A...
Source: Biochemical Genetics - January 24, 2024 Category: Genetics & Stem Cells Authors: Chong Xu Hehua Huang Hongmei Zou Yumeng Zhao Lu Liu Ruonan Chai Junli Zhang Source Type: research
FUS-Mediated CircFGFR1 Accelerates the Development of Papillary Thyroid Carcinoma by Stabilizing FGFR1 Protein
This study focuses on hsa_circ_0008016 (circFGFR1), a circular RNA significantly up-regulated in PTC cells. Silencing circFGFR1 inhibited PTC cell proliferation and increased cell apoptosis, suggesting its role in PTC progression. The RNA-binding protein FUS was identified as a promoter of circFGFR1 formation. While circFGFR1 does not influence FGFR1 mRNA translation, it inhibits ubiquitination and degradation of FGFR1 protein, prolonging its half-life. CircFGFR1 also interacts with protein CBL, inhibiting CBL-mediated ubiquitination of FGFR1 proteins. Rescue assays confirmed circFGFR1 promotes PTC cell growth through medi...
Source: Biochemical Genetics - January 23, 2024 Category: Genetics & Stem Cells Authors: Lu Zheng Tong Tang Zhitao Wang Chenyu Sun Xiao Chen Wanwan Li Benzhong Wang Source Type: research
Machine Learning Developed a MYC Expression Feature-Based Signature for Predicting Prognosis and Chemoresistance in Pancreatic Adenocarcinoma
Biochem Genet. 2024 Jan 21. doi: 10.1007/s10528-023-10625-0. Online ahead of print.ABSTRACTMYC has been identified to profoundly influence a wide range of pathologic processes in cancers. However, the prognostic value of MYC-related genes in pancreatic adenocarcinoma (PAAD) remains unclarified. Gene expression data and clinical information of PAAD patients were obtained from The Cancer Genome Atlas (TCGA) database (training set). Validation sets included GSE57495, GSE62452, and ICGC-PACA databases. LASSO regression analysis was used to develop a risk signature for survival prediction. Single-cell sequencing data from GSE15...
Source: Biochemical Genetics - January 21, 2024 Category: Genetics & Stem Cells Authors: Biao Dong Yueshan Zhang Han Gao Jia Liu Jiankun Li Source Type: research
Characterization and Statistical Optimization of Enterobatin Synthesized by Escherichia coli OQ866153
In this study, siderophores production by Escherichia coli OQ866153 was optimized using two-stage statistical approach involving Plackett-Burman design (PBD) and response surface methodology (RSM) using central composite design (CCD). Out of 23 variables, succinate, tryptophan, Na2HPO4, CaCl2, agitation, and KH2PO4 were found to have the most significant effect on siderophores production in the first optimization stage with the highest SU% of 43.67%. In the second stage, RSM using CCD was utilized, and the optimal conditions were determined to be 0.3 g/l succinate, 0 g/l tryptophan, 6 g/l Na2HPO4, 0.1 g/l CaCl2, 150 RPM ag...
Source: Biochemical Genetics - January 21, 2024 Category: Genetics & Stem Cells Authors: Mohamed T Khazaal Ahmed H I Faraag Marwa A Hamada Hoda H El-Hendawy Source Type: research
The Influence of ESR2 Gene Polymorphisms on Susceptibility to Hepatitis B Virus-Related Chronic Hepatitis, Liver Cirrhosis, and Hepatocellular Carcinoma
Biochem Genet. 2024 Jan 21. doi: 10.1007/s10528-023-10636-x. Online ahead of print.ABSTRACTHepatocellular carcinoma (HCC) represents an estrogen-dependent tumor. The action of estrogen is regulated via estrogen receptor (ER). Polymorphisms in ERα gene, ESR1, are known to be related to HCC susceptibility among people carrying chronic hepatitis B (CHB). But the effect of ERβ on HCC is still largely unclear, and studies about the genetic variability of ESR2 and HCC are rare. For understanding ESR2's effect on HCC, this work tested two polymorphisms in the ESR2 gene promoter as well as the associations with CHB, HCC, and hep...
Source: Biochemical Genetics - January 21, 2024 Category: Genetics & Stem Cells Authors: Zeng Jie Jiang Hongkun Yang Shi Yang Fengxun Liu Xin Meng Yijun Lu Yu Source Type: research
cGAS Deficiency Regulates the Phenotypic Polarization and Glycolysis of Microglia Through Lactylation in Hypoxic-Ischemic Encephalopathy Cell Model
Biochem Genet. 2024 Jan 21. doi: 10.1007/s10528-023-10631-2. Online ahead of print.ABSTRACTPromoting the M2 phenotype polarization of microglia is of great significance in alleviating hypoxic-ischemic encephalopathy (HIE). The umbilical artery blood sample was collected to evaluate the expression of cGAS, and the aberrant expressed cGAS was verified in the oxygen glucose deprivation (OGD) microglia which was established to mimic HIE in vitro. Then the regulating role of cGAS on the transformation of microglia M2 phenotype polarization and glycolysis was investigated. Moreover, the lactylation of cGAS in OGD treated microgl...
Source: Biochemical Genetics - January 21, 2024 Category: Genetics & Stem Cells Authors: Lisheng Wang Zhonghua Cai Qi Gu Changli Xu Source Type: research
Sodium Butyrate Inhibits the Malignant Proliferation of Colon Cancer Cells via the miR-183/DNAJB4 Axis
This study screened the IC50 value of butyrate on inhibition of CRC cells malignant progression. Its inhibitory effects were detected by MTT assay, colony formation experiment, Transwell migration experiment, and apoptosis evaluation by flow cytometry. Next, the expressions of miR-183 and DNAJB4 were, respectively, determined in butyrate treated and miR-183 analog or si-DNAJB4-transfected CRC cells to further detect the role of upregulated miR-183 or silencing DNAJB4 in CRC cells malignant progression. Subsequently, the targeted regulatory relationship between miR-183 and si-DNAJB4 was confirmed by bioinformatic prediction...
Source: Biochemical Genetics - January 20, 2024 Category: Genetics & Stem Cells Authors: Dingguo Pan Jingchao Hao Tao Wu Tao Shen Kun Yu Qiang Li Ruixi Hu Zhaoyu Yang Yunfeng Li Source Type: research
Enterotoxigenic Bacteroides fragilis (ETBF) Enhances Colorectal Cancer Cell Proliferation and Metastasis Through HDAC3/miR-139-3p Pathway
Biochem Genet. 2024 Jan 20. doi: 10.1007/s10528-023-10621-4. Online ahead of print.ABSTRACTEnterotoxigenic Bacteroides fragilis (ETBF) is believed to promote the malignant process of colorectal cancer (CRC), but the underlying molecular mechanism still needs to be revealed. CRC cells (SW480 and HCT-116) were treated with ETBF strain. Cell proliferation, invasion and, migration were evaluated by cell counting kit 8 assay, EdU assay, colony formation assay, transwell assay, and wound healing assay. Protein expression was analyzed by western blot. MicroRNA (miR)-139-3p and histone deacetylase 3 (HDAC3) expression levels in ti...
Source: Biochemical Genetics - January 20, 2024 Category: Genetics & Stem Cells Authors: Xiaoyong Wu Chengrui Yang Fangyuan Sun Yanzhong Zhang Yanliang Wang Xuzhao Li Fengxian Zheng Source Type: research
TFAP2C Activates CST1 Transcription to Facilitate Breast Cancer Progression and Suppress Ferroptosis
Biochem Genet. 2024 Jan 19. doi: 10.1007/s10528-023-10660-x. Online ahead of print.ABSTRACTCystatin SN (CST1) appears to have pro-tumor effects in breast cancer (BC) and is involved in ferroptosis; however, there is no report on the regulation of ferroptosis by CST1 for BC development. The purpose of this study is to investigate the functions and mechanisms operated by CST1 in BC development and ferroptosis. Transcription Factor Activator Protein 2γ (TFAP2C) and CST1 levels in BC tissues and estrogen receptor (ER)+ cells were quantified by RT-qPCR and western blotting. After knocking down TFAP2C and CST1 expression in MCF...
Source: Biochemical Genetics - January 19, 2024 Category: Genetics & Stem Cells Authors: Lin Yuan Di Zhou Weiwen Li Jianhua Guan Junda Li Bo Xu Source Type: research
Circ_0023990 Promotes the Proliferation, Invasion, and Glycolysis of Esophageal Squamous Cell Carcinoma Cells Via Targeting miR-6884-5p/PAK1 Axis
Biochem Genet. 2024 Jan 19. doi: 10.1007/s10528-024-10674-z. Online ahead of print.ABSTRACTCircular RNAs are emerging players in human cancers, including esophageal squamous cell carcinoma (ESCC). Herein, we assessed the expression level of circ_0023990 and explored the molecular mechanisms of circ_0023990 in ESCC. circ_0023990, miR-6884-5p, and PAK1 expressions in ESCC tissues and cells were detected by quantitative real-time polymerase chain reaction and western blot. ESCC cells were transfected with different constructs to alter the expression of circ_0023990, miR-6884-5p, and PAK1. The effect of circ_0023990 on the pro...
Source: Biochemical Genetics - January 19, 2024 Category: Genetics & Stem Cells Authors: Hui Tian Gaofeng Liang Qi Qin Chaoqun Yu Jinxian He Source Type: research
Genetic Investigation of the Trail Mechanism in Diabetic and Non-diabetic Obese Patients
Biochem Genet. 2024 Jan 19. doi: 10.1007/s10528-023-10624-1. Online ahead of print.ABSTRACTObesity is an important healthcare issue caused by abnormally increased adipose tissue because of energy-intake overcoming energy expenditure. Disturbances in the physiological function of adipose tissue mediate the development of diabetes. It is a metabolic disease that results from decreased insulin-levels and/or changes in the insulin action mechanism. Tumor Necrosis Factor-Associated Apoptosis-Inducing Ligand(TRAIL), which is a member of the Tumor Necrosis Factor(TNF)-family with an important role in adipose tissue biology, is in...
Source: Biochemical Genetics - January 19, 2024 Category: Genetics & Stem Cells Authors: Saadet Busra Aksoyer Sezgin Sermin Durak Faruk Celik Arezoo Gheybi Murat Diramali Ramazan Cakmak Ali Osman Gurol Ilhan Yaylim Umit Zeybek Source Type: research
