Molecular Cloning and Functional Analysis of Secretory Phospholipase A < sub > 2 < /sub > from Apostichopus japonicus
This study successfully amplified the AjPLA2 sequence. The total cDNA of AjPLA2 is 931 bp, including a 480 bp ORF that encodes 159 amino acids. The AjPLA2 protein includes a 16-aa signal peptide, a 5-aa precursor peptide and a 138-aa mature peptide. Homologous alignment showed that AjPLA2 and the sPLA2s from starfish have the typical domains of the Group IB sPLA2. And additional amino acid sequences were found around the β-Wing, which is different from the Group IB sPLA2. These results showed that AjPLA2 and sPLA2s from starfish all belong to a new group in the Group I sPLA2 family. AjPLA2 is widely distributed in sea cuc...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Cheng Li Lili Yang Zhongyun Zhang Ying Liu Xu Li Kai Yang Ming Chen Source Type: research
Molecular Characterization and Seasonal Variation in Expression of HSP70.1 Gene in Gangatiri Cattle and Its Comparison with Buffalo
Biochem Genet. 2024 Mar 18. doi: 10.1007/s10528-024-10739-z. Online ahead of print.ABSTRACTUnder tropical climate heat stress is a major challenge for livestock production. HSP70.1 is a ubiquitously expressed protein maintaining cellular machinery through proper folding of denatured proteins and prevents cellular apoptosis and protect cell from heat stress. Therefore, present investigation was undertaken to explore genetic variability in HSP70.1 gene in Gangatiri cattle, its comparison with buffalo sequences and differential expression in different season. The allelic variant was identified by sequencing amplified PCR prod...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Shanker Dayal Birendra Kumar Rajni Kumari Jyoti Kumar Pradeep Kumar Ray P C Chandran Amitava Dey Source Type: research
RBM24 Suppresses the Tumorigenesis of Glioblastoma by Stabilizing LATS1 mRNA
The objective of this study is to uncover the role of RBM24 in GBM and understand the underlying mechanism. The expression of RBM24 in GBM was initially analyzed using the Gene Expression Profiling Interactive Analysis (GEPIA). Subsequently, the RBM24 expression levels in clinical samples of GBM were examined, and the survival curves of GBM patients were plotted based on high- and low-expression levels of RBM24 using Kaplan-Meier (KM) plotter. In addition, RBM24 knockdown cell lines and overexpression vectors were created to assess the effects on proliferation, apoptosis, and invasion abilities. Finally, the binding level ...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Xuewen Lu Yong Xie Guolin Ding Wei Sun Hao Ye Source Type: research
Integrating Network Pharmacology and In Vitro Experimental Verification Revealing Bushenhuoxue Recipe Against Intrauterine Adhesions via PI3K-AKT Signaling Pathway
Biochem Genet. 2024 Mar 19. doi: 10.1007/s10528-024-10732-6. Online ahead of print.ABSTRACTWe aimed to investigate therapeutic effect of Bushenhuoxue recipe in intrauterine adhesions (IUA) and explore the underlying molecular mechanism via integrating network pharmacology and in vitro experimental verification. The active compounds and gene targets of Bushenhuoxue recipe were screened in the TCMSP database and the IUA-related genes were identified using GeneCards database by the keyword "Intrauterine adhesions". Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were conducted to reveal the unde...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Lingli Wang Lingzi Zhuang Yajing Lu Jin Jia Cailing Chen Ying Zhang Zhejing Hong Xiaohong Wang Source Type: research
Molecular Cloning and Functional Analysis of Secretory Phospholipase A < sub > 2 < /sub > from Apostichopus japonicus
This study successfully amplified the AjPLA2 sequence. The total cDNA of AjPLA2 is 931 bp, including a 480 bp ORF that encodes 159 amino acids. The AjPLA2 protein includes a 16-aa signal peptide, a 5-aa precursor peptide and a 138-aa mature peptide. Homologous alignment showed that AjPLA2 and the sPLA2s from starfish have the typical domains of the Group IB sPLA2. And additional amino acid sequences were found around the β-Wing, which is different from the Group IB sPLA2. These results showed that AjPLA2 and sPLA2s from starfish all belong to a new group in the Group I sPLA2 family. AjPLA2 is widely distributed in sea cuc...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Cheng Li Lili Yang Zhongyun Zhang Ying Liu Xu Li Kai Yang Ming Chen Source Type: research
Molecular Characterization and Seasonal Variation in Expression of HSP70.1 Gene in Gangatiri Cattle and Its Comparison with Buffalo
Biochem Genet. 2024 Mar 18. doi: 10.1007/s10528-024-10739-z. Online ahead of print.ABSTRACTUnder tropical climate heat stress is a major challenge for livestock production. HSP70.1 is a ubiquitously expressed protein maintaining cellular machinery through proper folding of denatured proteins and prevents cellular apoptosis and protect cell from heat stress. Therefore, present investigation was undertaken to explore genetic variability in HSP70.1 gene in Gangatiri cattle, its comparison with buffalo sequences and differential expression in different season. The allelic variant was identified by sequencing amplified PCR prod...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Shanker Dayal Birendra Kumar Rajni Kumari Jyoti Kumar Pradeep Kumar Ray P C Chandran Amitava Dey Source Type: research
RBM24 Suppresses the Tumorigenesis of Glioblastoma by Stabilizing LATS1 mRNA
The objective of this study is to uncover the role of RBM24 in GBM and understand the underlying mechanism. The expression of RBM24 in GBM was initially analyzed using the Gene Expression Profiling Interactive Analysis (GEPIA). Subsequently, the RBM24 expression levels in clinical samples of GBM were examined, and the survival curves of GBM patients were plotted based on high- and low-expression levels of RBM24 using Kaplan-Meier (KM) plotter. In addition, RBM24 knockdown cell lines and overexpression vectors were created to assess the effects on proliferation, apoptosis, and invasion abilities. Finally, the binding level ...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Xuewen Lu Yong Xie Guolin Ding Wei Sun Hao Ye Source Type: research
Integrating Network Pharmacology and In Vitro Experimental Verification Revealing Bushenhuoxue Recipe Against Intrauterine Adhesions via PI3K-AKT Signaling Pathway
Biochem Genet. 2024 Mar 19. doi: 10.1007/s10528-024-10732-6. Online ahead of print.ABSTRACTWe aimed to investigate therapeutic effect of Bushenhuoxue recipe in intrauterine adhesions (IUA) and explore the underlying molecular mechanism via integrating network pharmacology and in vitro experimental verification. The active compounds and gene targets of Bushenhuoxue recipe were screened in the TCMSP database and the IUA-related genes were identified using GeneCards database by the keyword "Intrauterine adhesions". Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis were conducted to reveal the unde...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Lingli Wang Lingzi Zhuang Yajing Lu Jin Jia Cailing Chen Ying Zhang Zhejing Hong Xiaohong Wang Source Type: research
Molecular Cloning and Functional Analysis of Secretory Phospholipase A < sub > 2 < /sub > from Apostichopus japonicus
This study successfully amplified the AjPLA2 sequence. The total cDNA of AjPLA2 is 931 bp, including a 480 bp ORF that encodes 159 amino acids. The AjPLA2 protein includes a 16-aa signal peptide, a 5-aa precursor peptide and a 138-aa mature peptide. Homologous alignment showed that AjPLA2 and the sPLA2s from starfish have the typical domains of the Group IB sPLA2. And additional amino acid sequences were found around the β-Wing, which is different from the Group IB sPLA2. These results showed that AjPLA2 and sPLA2s from starfish all belong to a new group in the Group I sPLA2 family. AjPLA2 is widely distributed in sea cuc...
Source: Biochemical Genetics - March 19, 2024 Category: Genetics & Stem Cells Authors: Cheng Li Lili Yang Zhongyun Zhang Ying Liu Xu Li Kai Yang Ming Chen Source Type: research
USP2 Promotes the Proliferation and Inflammation of Fibroblast-Like Synovial Cells in Rheumatoid Arthritis Through Deubiquitination of TRAF2
Biochem Genet. 2024 Mar 13. doi: 10.1007/s10528-024-10737-1. Online ahead of print.ABSTRACTRheumatoid arthritis (RA) is a prevalent inflammatory disorder affecting about 1% of the global population. The ubiquitin-specific protease 2 (USP2) is known to have a substantial influence on the regulation of several cellular processes. Both in vivo (using rats with collagen-induced arthritis, CIA) and in vitro (using human fibroblast-like synoviocytes, HFLS-RA) models of RA were used to examine the role of USP2 in RA. The proliferation of HFLS-RA cells was assessed using the cell counting kit 8 test and EdU staining. The technique...
Source: Biochemical Genetics - March 14, 2024 Category: Genetics & Stem Cells Authors: Xiuchan Liu Geng Zhang Lei Liu Guangyi Xiong Jun Liu Wei Wei Source Type: research
USP2 Promotes the Proliferation and Inflammation of Fibroblast-Like Synovial Cells in Rheumatoid Arthritis Through Deubiquitination of TRAF2
Biochem Genet. 2024 Mar 13. doi: 10.1007/s10528-024-10737-1. Online ahead of print.ABSTRACTRheumatoid arthritis (RA) is a prevalent inflammatory disorder affecting about 1% of the global population. The ubiquitin-specific protease 2 (USP2) is known to have a substantial influence on the regulation of several cellular processes. Both in vivo (using rats with collagen-induced arthritis, CIA) and in vitro (using human fibroblast-like synoviocytes, HFLS-RA) models of RA were used to examine the role of USP2 in RA. The proliferation of HFLS-RA cells was assessed using the cell counting kit 8 test and EdU staining. The technique...
Source: Biochemical Genetics - March 14, 2024 Category: Genetics & Stem Cells Authors: Xiuchan Liu Geng Zhang Lei Liu Guangyi Xiong Jun Liu Wei Wei Source Type: research
USP2 Promotes the Proliferation and Inflammation of Fibroblast-Like Synovial Cells in Rheumatoid Arthritis Through Deubiquitination of TRAF2
Biochem Genet. 2024 Mar 13. doi: 10.1007/s10528-024-10737-1. Online ahead of print.ABSTRACTRheumatoid arthritis (RA) is a prevalent inflammatory disorder affecting about 1% of the global population. The ubiquitin-specific protease 2 (USP2) is known to have a substantial influence on the regulation of several cellular processes. Both in vivo (using rats with collagen-induced arthritis, CIA) and in vitro (using human fibroblast-like synoviocytes, HFLS-RA) models of RA were used to examine the role of USP2 in RA. The proliferation of HFLS-RA cells was assessed using the cell counting kit 8 test and EdU staining. The technique...
Source: Biochemical Genetics - March 14, 2024 Category: Genetics & Stem Cells Authors: Xiuchan Liu Geng Zhang Lei Liu Guangyi Xiong Jun Liu Wei Wei Source Type: research
USP2 Promotes the Proliferation and Inflammation of Fibroblast-Like Synovial Cells in Rheumatoid Arthritis Through Deubiquitination of TRAF2
Biochem Genet. 2024 Mar 13. doi: 10.1007/s10528-024-10737-1. Online ahead of print.ABSTRACTRheumatoid arthritis (RA) is a prevalent inflammatory disorder affecting about 1% of the global population. The ubiquitin-specific protease 2 (USP2) is known to have a substantial influence on the regulation of several cellular processes. Both in vivo (using rats with collagen-induced arthritis, CIA) and in vitro (using human fibroblast-like synoviocytes, HFLS-RA) models of RA were used to examine the role of USP2 in RA. The proliferation of HFLS-RA cells was assessed using the cell counting kit 8 test and EdU staining. The technique...
Source: Biochemical Genetics - March 14, 2024 Category: Genetics & Stem Cells Authors: Xiuchan Liu Geng Zhang Lei Liu Guangyi Xiong Jun Liu Wei Wei Source Type: research
Linker Histone H1.4 Inhibits the Growth, Migration and EMT Process of Non-Small Cell Lung Cancer by Regulating ERK1/2 Expression
In this study, we found that overexpression of H1.4 significantly inhibited the cell viability, migration, invasion and epithelial-mesenchymal transition (EMT) processes, whereas silencing H1.4 by shRNA knockdown promoted these processes in NSCLC cell lines A549 and H1299. We further showed that H1.4 overexpression reduced ERK1/2 expression or its phosphorylation levels, while H1.4 knockdown increased ERK1/2 expression or phosphorylation levels in NSCLC. Furthermore, we demonstrated that H1.4 bound to the promoter of ERK1/2, and acted as a transcriptional suppressor to inhibit ERK1/2 expression in A549 or H1299 cells. Impo...
Source: Biochemical Genetics - March 13, 2024 Category: Genetics & Stem Cells Authors: Qian Chen Mengqi Yang Xinyue Duan Jie Zhang Fan Shi Rong Chen Yong Li Source Type: research
DR6 Augments Colorectal Cancer Cell Growth, Invasion, and Stemness by Activating AKT/NF- κB Pathway
This study aims to elucidate the role and mechanisms of Death Receptor 6 (DR6), a member of the tumor necrosis factor receptor superfamily, in the malignant progression of colorectal cancer (CRC). The association of DR6 expression levels and CRC patient survival was examined using the CRC cohort data from GEPIA database. The functional role of DR6 in CRC cells was investigated by performing loss-of-function and gain-of-function experiments based on CCK-8 proliferation assay, transwell migration and invasion assay, and sphere-forming assays. Xenograft model of CRC cells in nude mouse was established to evaluate the impact o...
Source: Biochemical Genetics - March 13, 2024 Category: Genetics & Stem Cells Authors: Jing Jia Yisen Huang Qiwei Chen Jianbin Hou Yan Liu Lifeng Xie Xinyu Li Chunkang Yang Source Type: research
