Overexpression of PIK3CG in Cancer Cells Promotes Lung Cancer Cell Migration and Metastasis Through Enhanced MMPs Expression and Neutrophil Recruitment and Activation
Biochem Genet. 2024 Apr 11. doi: 10.1007/s10528-024-10788-4. Online ahead of print.ABSTRACTMetastasis is a major cause of death in lung cancer. The aim of this study is to analyze the role and mechanism of PI3K catalytic subunit gamma (PIK3CG, also known as p110γ) in lung cancer cell migration and metastasis. Knockdown (KD) and overexpression (OE) of PIK3CG expression in lung cancer cell lines A549 and H1299 in vitro cultured was achieved. Two PIK3CG-specific inhibitors, Eganelisib and CAY10505, were used to treat A549 and H1299 cells. An experimental lung metastasis mouse model was constructed using tail vein injection o...
Source: Biochemical Genetics - April 11, 2024 Category: Genetics & Stem Cells Authors: Jinpeng Sun Zhenshan Zhang Binghui Xia Tianyu Yao Fengyue Ge Fengmei Yan Source Type: research
Dysregulation of miR-25-3p in Diabetic Nephropathy and Its Role in Inflammatory Response
Biochem Genet. 2024 Apr 11. doi: 10.1007/s10528-024-10781-x. Online ahead of print.ABSTRACTTo investigate the expression level of miR-25-3p in patients with type 2 diabetes mellitus (T2DM) and diabetic nephropathy (DN), and its effect on proliferation, apoptosis and inflammatory response of mesangial cells cultured with high glucose. Blood samples of all clinical subjects were collected for RT-qPCR analysis to detect serum miR-25-3p levels. Human mesangial cells (HMCs) cultured with high glucose were used to construct DN model in vitro. MTT assay, flow cytometry and ELISA were used to evaluate the effects of miR-25-3p on t...
Source: Biochemical Genetics - April 11, 2024 Category: Genetics & Stem Cells Authors: Huanzhen Chen Tongguan Tian Dan Wang Source Type: research
Analysis of Molecular Genetic Variants of Lgals4 in Esophageal Cancer: A Preliminary Report
Biochem Genet. 2024 Apr 12. doi: 10.1007/s10528-024-10780-y. Online ahead of print.ABSTRACTEsophageal cancer is the eighth most common cancer worldwide and fourth most common in developing countries. Altered glycosylation pattern of cell membrane molecules along with inflammation is a characteristic attribute of oncogenesis. Galectin-4, a tandem repeat galectin, has shown effect on cancer progression/metastasis in digestive system cancers. This role of galectin-4 can be attributed to variations in LGALS4, gene encoding galectin-4. The present case-control study was designed to analyze four intronic SNPs in LGALS4 with susc...
Source: Biochemical Genetics - April 11, 2024 Category: Genetics & Stem Cells Authors: Surmeet Kaur Rajiv Devgan Jagdeep Singh Namarta Kalia Jatinder Singh Manpreet Kaur Source Type: research
Overexpression of PIK3CG in Cancer Cells Promotes Lung Cancer Cell Migration and Metastasis Through Enhanced MMPs Expression and Neutrophil Recruitment and Activation
Biochem Genet. 2024 Apr 11. doi: 10.1007/s10528-024-10788-4. Online ahead of print.ABSTRACTMetastasis is a major cause of death in lung cancer. The aim of this study is to analyze the role and mechanism of PI3K catalytic subunit gamma (PIK3CG, also known as p110γ) in lung cancer cell migration and metastasis. Knockdown (KD) and overexpression (OE) of PIK3CG expression in lung cancer cell lines A549 and H1299 in vitro cultured was achieved. Two PIK3CG-specific inhibitors, Eganelisib and CAY10505, were used to treat A549 and H1299 cells. An experimental lung metastasis mouse model was constructed using tail vein injection o...
Source: Biochemical Genetics - April 11, 2024 Category: Genetics & Stem Cells Authors: Jinpeng Sun Zhenshan Zhang Binghui Xia Tianyu Yao Fengyue Ge Fengmei Yan Source Type: research
Dysregulation of miR-25-3p in Diabetic Nephropathy and Its Role in Inflammatory Response
Biochem Genet. 2024 Apr 11. doi: 10.1007/s10528-024-10781-x. Online ahead of print.ABSTRACTTo investigate the expression level of miR-25-3p in patients with type 2 diabetes mellitus (T2DM) and diabetic nephropathy (DN), and its effect on proliferation, apoptosis and inflammatory response of mesangial cells cultured with high glucose. Blood samples of all clinical subjects were collected for RT-qPCR analysis to detect serum miR-25-3p levels. Human mesangial cells (HMCs) cultured with high glucose were used to construct DN model in vitro. MTT assay, flow cytometry and ELISA were used to evaluate the effects of miR-25-3p on t...
Source: Biochemical Genetics - April 11, 2024 Category: Genetics & Stem Cells Authors: Huanzhen Chen Tongguan Tian Dan Wang Source Type: research
C1q/Tumor Necrosis Factor-Related Protein-9 Enhances Macrophage Cholesterol Efflux and Improves Reverse Cholesterol Transport via AMPK Activation
Biochem Genet. 2024 Apr 10. doi: 10.1007/s10528-024-10761-1. Online ahead of print.ABSTRACTCholesterol efflux from foam cells in atherosclerotic plaques is crucial for reverse cholesterol transport (RCT), an important antiatherogenic event. ATP-binding cassette (ABC) transporters, ABCA1 and ABCG1, are key receptors in the cholesterol efflux pathway. C1q/tumor necrosis factor-related protein-9 (CTRP9) is a newly discovered adipokine and exhibits an atheroprotective activity. However, the role of CTRP9 in RCT still remains unknown. In this work, we investigated the effect of subcutaneous administration of CTRP9 protein on RC...
Source: Biochemical Genetics - April 10, 2024 Category: Genetics & Stem Cells Authors: Xiaosu Song Gaizhen Liu Yunfei Bin Rui Bai Bin Liang Huiyu Yang Source Type: research
The Expression Analysis of Long Non-coding RNAs Related to Wnt/ β-Catenin Signaling in Pancreatic Cancer Patients
In this study, we investigated and compared the expression of Wnt/β-catenin signaling-related ZFAS1 and HCG11 lncRNAs, and their targets, CTNNB1 and IGF2BP1 genes in the blood of patients with PC and healthy individuals. A total of 47 PC patients and 50 healthy individuals participated in this study. RNA was extracted from the peripheral blood samples of participants, and cDNA was synthesized. The expression level of the selected genes was quantified by real-time PCR. The expression of HCG11 lncRNA and CTNNB1 genes in patients with PC was significantly upregulated compared to healthy individuals, and the expression of the...
Source: Biochemical Genetics - April 9, 2024 Category: Genetics & Stem Cells Authors: Fatemeh Katoozian Zahra Abedi Kichi Roya Sharifi Zeinab Shirvani-Farsani Source Type: research
A Cuproptosis-Related gene Signature as a Prognostic Biomarker in Thyroid Cancer Based on Transcriptomics
Biochem Genet. 2024 Apr 9. doi: 10.1007/s10528-024-10767-9. Online ahead of print.ABSTRACTThyroid cancer (THCA) is the most prevalent endocrine tumor, and its incidence continues to increase every year. However, the processes underlying the aggressive progression of thyroid cancer are unknown. We concentrated on the prognostic and biological importance of thyroid cancer cuproptosis-related genes in this investigation. Genomic and clinical data were obtained from the UCSC XENA website, and cuproptosis-related genes were obtained from the FerrDb website. We performed differential expression analysis and Cox regression analys...
Source: Biochemical Genetics - April 9, 2024 Category: Genetics & Stem Cells Authors: Zirui Guan Hongyong Wang Mingyan Tian Source Type: research
A Cuproptosis-Related gene Signature as a Prognostic Biomarker in Thyroid Cancer Based on Transcriptomics
Biochem Genet. 2024 Apr 9. doi: 10.1007/s10528-024-10767-9. Online ahead of print.ABSTRACTThyroid cancer (THCA) is the most prevalent endocrine tumor, and its incidence continues to increase every year. However, the processes underlying the aggressive progression of thyroid cancer are unknown. We concentrated on the prognostic and biological importance of thyroid cancer cuproptosis-related genes in this investigation. Genomic and clinical data were obtained from the UCSC XENA website, and cuproptosis-related genes were obtained from the FerrDb website. We performed differential expression analysis and Cox regression analys...
Source: Biochemical Genetics - April 9, 2024 Category: Genetics & Stem Cells Authors: Zirui Guan Hongyong Wang Mingyan Tian Source Type: research
The Expression Analysis of Long Non-coding RNAs Related to Wnt/ β-Catenin Signaling in Pancreatic Cancer Patients
In this study, we investigated and compared the expression of Wnt/β-catenin signaling-related ZFAS1 and HCG11 lncRNAs, and their targets, CTNNB1 and IGF2BP1 genes in the blood of patients with PC and healthy individuals. A total of 47 PC patients and 50 healthy individuals participated in this study. RNA was extracted from the peripheral blood samples of participants, and cDNA was synthesized. The expression level of the selected genes was quantified by real-time PCR. The expression of HCG11 lncRNA and CTNNB1 genes in patients with PC was significantly upregulated compared to healthy individuals, and the expression of the...
Source: Biochemical Genetics - April 9, 2024 Category: Genetics & Stem Cells Authors: Fatemeh Katoozian Zahra Abedi Kichi Roya Sharifi Zeinab Shirvani-Farsani Source Type: research
LncRNA AC005165.1 Alleviates IL-1 β-Induced Osteoarthritis via miR-199a-3p/TXNIP Axis
This study was done to explore the role and mechanism of the lncRNA AC005165.1 in the cell model of interleukin-1β (IL)-1β-treated chondrocytes. This study recruited 20 surgically treated OA patients and 12 age- and gender-matched controls. Real-time reverse transcription quantitative polymerase chain reaction was used to examine the expression levels of AC005165.1, miR-199a-3p, and thioredoxin-interacting protein (TXNIP) in articular cartilage of patients and IL-1β-treated human chondrocytes. Cell viability and apoptosis were evaluated by cell counting kit-8 and flow cytometry assays, respectively. The protein levels o...
Source: Biochemical Genetics - April 8, 2024 Category: Genetics & Stem Cells Authors: Xi Gu Tian Xie Source Type: research
LncRNA AC005165.1 Alleviates IL-1 β-Induced Osteoarthritis via miR-199a-3p/TXNIP Axis
This study was done to explore the role and mechanism of the lncRNA AC005165.1 in the cell model of interleukin-1β (IL)-1β-treated chondrocytes. This study recruited 20 surgically treated OA patients and 12 age- and gender-matched controls. Real-time reverse transcription quantitative polymerase chain reaction was used to examine the expression levels of AC005165.1, miR-199a-3p, and thioredoxin-interacting protein (TXNIP) in articular cartilage of patients and IL-1β-treated human chondrocytes. Cell viability and apoptosis were evaluated by cell counting kit-8 and flow cytometry assays, respectively. The protein levels o...
Source: Biochemical Genetics - April 8, 2024 Category: Genetics & Stem Cells Authors: Xi Gu Tian Xie Source Type: research
Moringa oleifera and Its Biochemical Compounds: Potential Multi-targeted Therapeutic Agents Against COVID-19 and Associated Cancer Progression
Biochem Genet. 2024 Apr 7. doi: 10.1007/s10528-024-10758-w. Online ahead of print.ABSTRACTThe Coronavirus disease-2019 (COVID-19) pandemic is a global concern, with updated pharmacological therapeutic strategies needed. Cancer patients have been found to be more susceptible to severe COVID-19 and death, and COVID-19 can also lead to cancer progression. Traditional medicinal plants have long been used as anti-infection and anti-inflammatory agents, and Moringa oleifera (M. oleifera) is one such plant containing natural products such as kaempferol, quercetin, and hesperetin, which can reduce inflammatory responses and compli...
Source: Biochemical Genetics - April 7, 2024 Category: Genetics & Stem Cells Authors: Ali Yousefi Rad Ali Asghar Rastegari Kahin Shahanipour Ramesh Monajemi Source Type: research
Predictive Value and Immunological Role of the HSPA5 Gene in Cervical Cancer
Biochem Genet. 2024 Apr 8. doi: 10.1007/s10528-024-10782-w. Online ahead of print.ABSTRACTCervical cancer (CC) ranks fourth among women's malignancies worldwide and seriously affects women's health. HSPA5 is a heat shock protein, also known as glucose regulatory protein 78 (GRP78). Upregulation of HSPA5 has been reported to be closely associated with multiple types of tumors. However, the specific role of HSPA5 in cervical cancer has not been discovered. In our study, we explored the prognostic value of HSPA5 in CC. Here, we analyzed the (TCGA) and (UCSC) databases, the analysis of HSPA5 in many tumors types was conducted ...
Source: Biochemical Genetics - April 7, 2024 Category: Genetics & Stem Cells Authors: Yingying Bai Yandong Miao Jiangtao Wang Jian Gan Jiang Feng Source Type: research
Screening of Spinal Muscular Atrophy Carriers and Prenatal Diagnosis in Pregnant Women in Yancheng, China
Biochem Genet. 2024 Apr 6. doi: 10.1007/s10528-024-10775-9. Online ahead of print.ABSTRACTSpinal muscular atrophy (SMA) is a neuromuscular disorder with an autosomal recessive inheritance pattern. Patients with severe symptoms may suffer respiratory failure, leading to death. The homozygous deletion of exon 7 in the SMN1 gene accounts for nearly 95% of all cases. Population carrier screening for SMA and prenatal diagnosis by amniocentesis for high-risk couples can assist in identifying the risk of fetal disease. We provided the SMA carrier screening process to 55,447 pregnant women in Yancheng from October 2020 to December...
Source: Biochemical Genetics - April 6, 2024 Category: Genetics & Stem Cells Authors: Huilin Sun Jianli Zheng Qing'e Zhang Feifei Ying Yadong Fu Yongjuan Guan Jing Wu Yueyun Zhou Jingjing Dong Mengjun Xu Fangfang Yang Ning An Ning Shi Lu Zhang Shu Zhu Jianbing Liu Min Li Source Type: research
